LOCUS       NM_001107684            6311 bp    mRNA    linear   ROD 04-APR-2024
DEFINITION  Rattus norvegicus Rap guanine nucleotide exchange factor 2
            (Rapgef2), transcript variant 2, mRNA.
ACCESSION   NM_001107684 XM_001069856 XM_227301
VERSION     NM_001107684.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Garza Carbajal,A., Ebersberger,A., Thiel,A., Ferrari,L., Acuna,J.,
            Brosig,S., Isensee,J., Moeller,K., Siobal,M., Rose-John,S.,
            Levine,J., Schaible,H.G. and Hucho,T.
  TITLE     Oncostatin M induces hyperalgesic priming and amplifies signaling
            of cAMP to ERK by RapGEF2 and PKA
  JOURNAL   J Neurochem 157 (6), 1821-1837 (2021)
   PUBMED   32885411
  REMARK    GeneRIF: Oncostatin M induces hyperalgesic priming and amplifies
            signaling of cAMP to ERK by RapGEF2 and PKA.
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   Emery,A.C., Eiden,M.V. and Eiden,L.E.
  TITLE     Separate cyclic AMP sensors for neuritogenesis, growth arrest, and
            survival of neuroendocrine cells
  JOURNAL   J Biol Chem 289 (14), 10126-10139 (2014)
   PUBMED   24567337
  REMARK    GeneRIF: Following cAMP elevation, neuritogenic cyclic AMP
            sensor/Rapgef2 is activated for signaling to ERK to mediate
            neuritogenesis, whereas Epac2 is activated for signaling to the MAP
            kinase p38 to mediate growth arrest.
REFERENCE   3  (bases 1 to 6311)
  AUTHORS   Monteiro,A.C., Sumagin,R., Rankin,C.R., Leoni,G., Mina,M.J.,
            Reiter,D.M., Stehle,T., Dermody,T.S., Schaefer,S.A., Hall,R.A.,
            Nusrat,A. and Parkos,C.A.
  TITLE     JAM-A associates with ZO-2, afadin, and PDZ-GEF1 to activate Rap2c
            and regulate epithelial barrier function
  JOURNAL   Mol Biol Cell 24 (18), 2849-2860 (2013)
   PUBMED   23885123
REFERENCE   4  (bases 1 to 6311)
  AUTHORS   Emery,A.C., Eiden,M.V., Mustafa,T. and Eiden,L.E.
  TITLE     Rapgef2 connects GPCR-mediated cAMP signals to ERK activation in
            neuronal and endocrine cells
  JOURNAL   Sci Signal 6 (281), ra51 (2013)
   PUBMED   23800469
  REMARK    GeneRIF: Data indicate that knockdown of Rapgef2 ablates
            cAMP-dependent ERK activation and neuritogenesis in neuronal cell
            line Neuroscreen-1 (NS-1).
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6311)
  AUTHORS   Gloerich,M., ten Klooster,J.P., Vliem,M.J., Koorman,T.,
            Zwartkruis,F.J., Clevers,H. and Bos,J.L.
  TITLE     Rap2A links intestinal cell polarity to brush border formation
  JOURNAL   Nat Cell Biol 14 (8), 793-801 (2012)
   PUBMED   22797597
REFERENCE   6  (bases 1 to 6311)
  AUTHORS   Kawajiri,A., Itoh,N., Fukata,M., Nakagawa,M., Yamaga,M.,
            Iwamatsu,A. and Kaibuchi,K.
  TITLE     Identification of a novel beta-catenin-interacting protein
  JOURNAL   Biochem Biophys Res Commun 273 (2), 712-717 (2000)
   PUBMED   10873669
REFERENCE   7  (bases 1 to 6311)
  AUTHORS   Pham,N., Cheglakov,I., Koch,C.A., de Hoog,C.L., Moran,M.F. and
            Rotin,D.
  TITLE     The guanine nucleotide exchange factor CNrasGEF activates ras in
            response to cAMP and cGMP
  JOURNAL   Curr Biol 10 (9), 555-558 (2000)
   PUBMED   10801446
  REMARK    Erratum:[Curr Biol 2000 Jul 27-Aug 10;10(15):R577-8]
REFERENCE   8  (bases 1 to 6311)
  AUTHORS   de Rooij,J., Boenink,N.M., van Triest,M., Cool,R.H.,
            Wittinghofer,A. and Bos,J.L.
  TITLE     PDZ-GEF1, a guanine nucleotide exchange factor specific for Rap1
            and Rap2
  JOURNAL   J Biol Chem 274 (53), 38125-38130 (1999)
   PUBMED   10608883
REFERENCE   9  (bases 1 to 6311)
  AUTHORS   Liao,Y., Kariya,K., Hu,C.D., Shibatohge,M., Goshima,M., Okada,T.,
            Watari,Y., Gao,X., Jin,T.G., Yamawaki-Kataoka,Y. and Kataoka,T.
  TITLE     RA-GEF, a novel Rap1A guanine nucleotide exchange factor containing
            a Ras/Rap1A-associating domain, is conserved between nematode and
            humans
  JOURNAL   J Biol Chem 274 (53), 37815-37820 (1999)
   PUBMED   10608844
REFERENCE   10 (bases 1 to 6311)
  AUTHORS   Ohtsuka,T., Hata,Y., Ide,N., Yasuda,T., Inoue,E., Inoue,T.,
            Mizoguchi,A. and Takai,Y.
  TITLE     nRap GEP: a novel neural GDP/GTP exchange protein for rap1 small G
            protein that interacts with synaptic scaffolding molecule (S-SCAM)
  JOURNAL   Biochem Biophys Res Commun 265 (1), 38-44 (1999)
   PUBMED   10548487
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CH473976.2.
            
            On or before Oct 4, 2007 this sequence version replaced
            XM_227301.4, XM_001069856.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: partial sample support SAMD00132261, SAMD00132262
                              [ECO:0000350]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q33"
     gene            1..6311
                     /gene="Rapgef2"
                     /gene_synonym="nRap GEP; PDZ-GEF1; RA-GEF-1"
                     /note="Rap guanine nucleotide exchange factor 2"
                     /db_xref="GeneID:310533"
                     /db_xref="RGD:1306434"
     exon            1..168
                     /gene="Rapgef2"
                     /gene_synonym="nRap GEP; PDZ-GEF1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            169..346
                     /gene="Rapgef2"
                     /gene_synonym="nRap GEP; PDZ-GEF1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            347..505
                     /gene="Rapgef2"
                     /gene_synonym="nRap GEP; PDZ-GEF1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             422..4306
                     /gene="Rapgef2"
                     /gene_synonym="nRap GEP; PDZ-GEF1; RA-GEF-1"
                     /note="isoform 2 is encoded by transcript variant 2;
                     neural RAP guanine nucleotide exchange protein; Rap
                     guanine nucleotide exchange factor (GEF) 2; PDZ
                     domain-containing guanine nucleotide exchange factor 1;
                     CNrasGEF; cyclic nucleotide ras GEF; ras/Rap1-associating
                     GEF-1; rap guanine nucleotide exchange factor 6-like"
                     /codon_start=1
                     /product="rap guanine nucleotide exchange factor 2 isoform
                     2"
                     /protein_id="NP_001101154.1"
                     /db_xref="GeneID:310533"
                     /db_xref="RGD:1306434"
                     /translation="MGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQV
                     EKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIE
                     DFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFL
                     EEFENNLEREKMGGHLRLLNIACAAKAKRRSMTLTKPSREAPLPFILLGGSEKGFGIF
                     VDSVDSSSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVF
                     KELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVG
                     GRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPAALPVSGTLSSSN
                     PDLLQSHHRILDFSTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVT
                     ATPEQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDA
                     QELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKKF
                     EEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAP
                     VARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLSGQNLQPPVIPLFPVIKKD
                     LTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRSLSQGSANAT
                     VLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQC
                     EPATSTLPKNPADRKPVKSETSPVAPRAGPQQKAQPQQPLAPPQPSHKVSQGLQVPAV
                     SLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRKQAEDTISNASSQLSSP
                     PTSPQSSPRKGYALALSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRH
                     SVSIVESNLGVGRMERRTLMEPDQYSLGSYAPVSESRGLYAAATVISSPSTEELSHDQ
                     GDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDATGIWAS
                     GGHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGG
                     KDVSTEAESSSMVPVTTEEAKPVPMPAHIAVTPSTTKGLIARKEGRYREPPPTPPGYV
                     GIPIADFPEGPCHPARKPPDYNVALQRSRMVARPTEAPAPGQTPPAATASRPGSKPQW
                     HKPSDADPRLAPFQPQGFAGAEEDEDEQVSAV"
     exon            506..658
                     /gene="Rapgef2"
                     /gene_synonym="nRap GEP; PDZ-GEF1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            659..823
                     /gene="Rapgef2"
                     /gene_synonym="nRap GEP; PDZ-GEF1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            824..931
                     /gene="Rapgef2"
                     /gene_synonym="nRap GEP; PDZ-GEF1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            932..1135
                     /gene="Rapgef2"
                     /gene_synonym="nRap GEP; PDZ-GEF1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1244
                     /gene="Rapgef2"
                     /gene_synonym="nRap GEP; PDZ-GEF1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..1491
                     /gene="Rapgef2"
                     /gene_synonym="nRap GEP; PDZ-GEF1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1492..1649
                     /gene="Rapgef2"
                     /gene_synonym="nRap GEP; PDZ-GEF1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1650..1890
                     /gene="Rapgef2"
                     /gene_synonym="nRap GEP; PDZ-GEF1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1891..2274
                     /gene="Rapgef2"
                     /gene_synonym="nRap GEP; PDZ-GEF1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2275..2486
                     /gene="Rapgef2"
                     /gene_synonym="nRap GEP; PDZ-GEF1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2487..2610
                     /gene="Rapgef2"
                     /gene_synonym="nRap GEP; PDZ-GEF1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2611..2834
                     /gene="Rapgef2"
                     /gene_synonym="nRap GEP; PDZ-GEF1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2835..3041
                     /gene="Rapgef2"
                     /gene_synonym="nRap GEP; PDZ-GEF1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3042..3194
                     /gene="Rapgef2"
                     /gene_synonym="nRap GEP; PDZ-GEF1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3195..3420
                     /gene="Rapgef2"
                     /gene_synonym="nRap GEP; PDZ-GEF1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3421..3980
                     /gene="Rapgef2"
                     /gene_synonym="nRap GEP; PDZ-GEF1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3981..4280
                     /gene="Rapgef2"
                     /gene_synonym="nRap GEP; PDZ-GEF1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4281..6311
                     /gene="Rapgef2"
                     /gene_synonym="nRap GEP; PDZ-GEF1; RA-GEF-1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtggactata tggatgaaaa tgaagaatat tttcagcgcc aagcctccca cagacagtct
       61 cgaagaagat ttagaaaaat caaccagaaa ggtgaaagac agacaattat cgacacagtg
      121 gacccttacc ctgtgggcaa gccacctcta cctagaggct atcacacggc cacggagagc
      181 gaggctggtg acatggacct aagtggactg ccagagacgg cagttgactc tgaggatgat
      241 gacgatgagg aggacatcga gagagcatca gaccctctga tgagcaggga catcgtgagg
      301 gactgcctgg agaaggaccc gatagacagg actgacgatg acatagctgg attcctggtc
      361 agtgattctc aatggttcgg tggaggtgac gtatccagat gggaaagcag aaatactgtg
      421 catgggaaac agctttggtg tttcgcccac catggacaaa gagtacatga aaggagtcat
      481 gaggacaaag gtggatgact gccagtttgt ctgcattgcc cagcaagatt actgccgtat
      541 tttgaaccaa gtagaaaaga acatgcaaaa agttgaggaa gaaggagaga ttgtaatggt
      601 gaaggagcac cgagaacttg atcggactgg aacaaggaag ggccacatcg tcatcaaggg
      661 cacctcagag aggctaacca tgcatttggt ggaagagcat tctgtagtgg acccaacatt
      721 catagaagac ttcctgttga cataccggac tttcctttcc agcccaatgg aagtgggcaa
      781 gaagttattg gagtggttca atgacccgag cctcagggat aaggtgacac gggtagtatt
      841 actgtgggtg aataatcact tcaatgactt tgaaggagat cctgcaatga ctcgatttct
      901 agaagaattt gaaaataatc tggaaagaga gaaaatgggt ggacatctaa ggctgctaaa
      961 cattgcatgt gcagcgaaag ccaaacgaag gtcgatgacg ttaacaaagc cgtcccggga
     1021 ggcgcctctg cccttcatcc tgctgggagg ctctgaaaag ggcttcggca tctttgtgga
     1081 cagtgttgac tcatcgagca aagccactga agcaggcttg aagcgtgggg atcagatatt
     1141 agaagtgaat ggtcaaaatt ttgaaaatat ccaactttca aaggcaatgg aaattcttag
     1201 aaataacaca cacctgtcca tcactgtgaa aaccaactta tttgtattta aagaacttct
     1261 gacgagattg tctgaagaga aaagaaatgg tgccccccac cttcctaaaa ttggagacat
     1321 caagaaggcc agtcgctact ccataccgga tcttgctgta gatgtagaac aggtgatagg
     1381 cctcgagaag gtcaacaaga aaagcaaagc caacactgtg gggggaagaa acaagctaaa
     1441 gaaaatactc gacaagactc ggatcagtat cttacctcag aaaccataca atgatattgg
     1501 gattggtcag tctcaagatg atagcatcgt aggactgagg cagaccaagc acattccagc
     1561 tgccctgcct gtcagtggaa cgctgtcttc cagtaaccct gacttgctgc agtcacacca
     1621 ccggattctg gacttcagca ctactcctga cttgccagat caggtactga gagttttcaa
     1681 ggctgaccag caaagcagat acatcatgat cagtaaggac accacagcaa aggaagtggt
     1741 catccaggcc atcagagagt ttgctgttac tgccacccca gagcagtatt ccctctgtga
     1801 ggtctcggtc acgccagagg gagttatcaa acaacgaagg ctcccagatc aactttccaa
     1861 gcttgctgac agaatacagc tgagcgggag gtattatctg aaaaacaaca tggaaacaga
     1921 aacgctttgc tcagatgaag atgcccagga gctgttgcga gagagccaaa tttcacttct
     1981 tcagctcagc actgtggagg tcgccacaca gctctccatg aggaactttg aactcttccg
     2041 caacattgag cctactgaat acatagatga tttatttaaa ctcaaatcaa aaaccagctg
     2101 tgccaaccta aagaaatttg aggaagtcat taaccaggaa acattttggg tggcttctga
     2161 aattctccga gagacaaacc agctgaagag aatgaagatc attaagcatt tcatcaagat
     2221 tgccttgcac tgtagggaat gcaagaattt taactccatg tttgcaatca tcagtggcct
     2281 aaacctggca ccagtggcaa gactacggac cacctgggaa aaacttccca ataaatatga
     2341 aaagctcttc caggacctac aagacctgtt tgatccttcc agaaacatgg ctaagtatcg
     2401 caatgtcctg agtggccaga acttacagcc tcctgtcatt cccctattcc ccgtcatcaa
     2461 gaaggacctc acgtttctcc atgagggaaa tgactcaaaa gtagatgggc tggtcaactt
     2521 tgagaagctg aggatgattg caaaggaaat ccgtcatgtt ggccgaatgg cctcagtcaa
     2581 catggaccct gcccttatgt tcagaactcg gtcgctgagt cagggcagtg cgaatgccac
     2641 agtgctggat gttgctcaga caggcgggca taaaaagcgg gtacgccgta gttccttcct
     2701 caatgccaaa aagctgtatg aagatgccca aatggctcgg aaagtgaagc aatacctgtc
     2761 caacctggag ctggagatgg acgaggagag tcttcagaca ctgtcacttc agtgtgaacc
     2821 agcaaccagc acattgccca agaaccctgc agacaggaag cctgtcaagt ccgagacctc
     2881 cccagtggct ccacgtgcag ggccccagca gaaagcgcag cctcagcagc cgttggcacc
     2941 ccctcaacca tcgcataaag tcagccaggg gctgcaggtg cctgctgtgt ccctctaccc
     3001 ctcacgaaag aaggtgccag tgaaggatct gccgcccttt ggcataaact ctccacaagc
     3061 tttaaagaaa atcctgtcct tgtctgaaga agggagtttg gagcgtcata ggaagcaagc
     3121 agaagacaca atatcaaacg catcctcaca gctctcatca ccccccacct cacctcagag
     3181 ctctccaagg aaagggtatg cattggctct cagtgggact gtggataatt tctcagattc
     3241 tggtcacagt gagatttctt cacgatccag tattgtcagc aattcttctt ttgactcggt
     3301 gccagtctct ctgcatgatg aacggcgcca gaggcattct gtcagcattg tggagagcaa
     3361 cctaggggtg ggcaggatgg agcggcggac cctgatggag cctgatcaat acagcctagg
     3421 atcatatgcc ccagtgtctg aaagtcgagg cctgtatgcc gcagcgacag tcatctcttc
     3481 tcctagcaca gaggagcttt cccacgacca aggggaccgt gcttctctcg atgctgctga
     3541 cagcggtcgg gggagctgga catcatgctc aagtggttct catgacaaca tacagaccat
     3601 ccagcaccag agaagctggg aaacactgcc atttgggcac actcactttg actattcagg
     3661 ggatgcgaca ggtatatggg cctccggcgg ccatatggac caaatcatgt tttctgatca
     3721 tagcacaaaa tataatagac agaatcagag tagagagagt cttgaacaag cccaatcccg
     3781 ggcaagctgg gcgtcttcca cgggctactg gggagaagac tcagaaggcg acacgggcac
     3841 tatcaagcga aggggtggga aggatgtctc cactgaggca gagagcagca gcatggtgcc
     3901 tgtgactaca gaggaagcta agcctgtccc tatgcctgcc cacatagccg tgacgcccag
     3961 cactaccaag ggactcatcg cacgaaagga aggcaggtac cgggagccgc ctcccacacc
     4021 tccaggctac gtgggcattc ccattgccga tttcccagaa gggccttgcc accccgccag
     4081 gaagcccccg gattacaacg tggccctgca gcggtcccgc atggtggcac ggcccactga
     4141 ggcccccgca ccaggccaga cgccgccggc agccacagcc agccggccgg gcagcaaacc
     4201 gcagtggcac aagcccagcg acgcagaccc acgcctcgcg cccttccagc cgcagggctt
     4261 cgccggagcg gaggaggacg aagatgaaca agtgtctgct gtttgaggca caggctcctt
     4321 gagtgggcta cccaaaggag agcacaagaa gacgtcccaa gcattggagc cttggcacgc
     4381 acatctgagg atggtggacc agtttgcctc cttccttccc tgccttaaag cagcatgggg
     4441 cttcttctcc ccttcttcct ttcccctttg catgtgaagt actgtgaaga aactgccctg
     4501 gcactttgca gacttgttgc ttgaaatgca cagcccagca gtctctgagc tgctgcctgc
     4561 cacgtcacgc agtatcattc caaattccaa gatcgtcaca aggcgactcc ctctggcggc
     4621 acttctccat gcctggaagg agtttccagt cttcctctta gatttcagtc cagtcctaga
     4681 cctcgatctc attcggaaga tgagaaaagc tagcctttga actacttggg gtctcgaacc
     4741 caccaaggaa gacaaagaca gataagaaat ccttagagta cagtgcttgt ccacttgttt
     4801 acaatatcct ttaaaaaaat aagtttaaag attgtgttca gagagtaaat gttatatcca
     4861 tttaatgatt acagtattat tttgaacctt aagtaggatt gccagcctga gtttctgaaa
     4921 aaccaaatat gccggacagg gtttggccgc accaagggaa gacctgggca gtgaccctgt
     4981 cttcccatgt ccttctggcc ttgcccgtga agtgccctat cccagaagca tcaaacgtta
     5041 gccaattact taaataccaa gactcctcac ctgctccttc cccagtgggc gaggctctaa
     5101 tgtaaaactg tttgcacatg gccaggtcgg gaactaggac tcgtgtggcc tgtctgagcc
     5161 ttatgaggca ggatggcgtt atttgagagt gtgtcctgct caattacaat ggatggcaac
     5221 ccctgttttt aagttatggg tttttttttt tttgtttttg tttttgtttt ttgttttttt
     5281 tttcaagttt tgttaattta gggttttaga gctttttgtt tttatttttt aaaagaaaca
     5341 tttataactg gatagcattg cagtgaagca gcttggggtg ttgggaaaaa tgccagctgt
     5401 ttgtactgct ctttcaagac agcctcccct tgttggattg gcaccagagt gtctttaaac
     5461 atggttaaag gtcacaaaca tggttaagta caccagcctt tacaaagtgg gtcagtcacg
     5521 aagactgacc cgtgagtggc tttgtggacg ctgcctgtgg aggaggcgaa agtgtacacc
     5581 agtctgctca tagctgctat tagccctaga atgactgaaa acgaccccgc ctttctattt
     5641 ttgtgttgtt tttgcacaga ctccggaaaa gtgaaggctg ccaatccgag tagtactcag
     5701 atgtgagggg ctgctgatct tggatttttt tccattaaat tcagctgatc atattgatca
     5761 gtagataaac gtaaatagct tcaaatttta aaggtcgaat tgcagtgttt tttcactgtg
     5821 tcaaacaatg tcagtgcttt atttaataat tctcttctat gtcatggcgt tcgtctactt
     5881 atgtatcaca ttgtcacttc cgcatttgta attttacatg taatatgcat tattgccagt
     5941 tttactctat aggctatgga cctcatgtgc atatagaaag acaaaactct agctctacca
     6001 caaattgcac agatgttatc taaacattaa gtaattgtag aacataggac tgctaatctc
     6061 agttcgctct gtgatgtcaa gtgcagaatg taaaattaac tggtgatttc ctcatacttt
     6121 tgataccact tgtacctgta tgtcttttag aaagacattg gtggagtctg tatccctttt
     6181 gtatttttaa tacaataatt gtacatattg gttctatttt tgttgaagat ggtagaaatg
     6241 tactatgttt atgcttctac atccagtttg tacaagctgg aaaataaata aatataagat
     6301 aaagccttgt c
//
