LOCUS       NM_001401396            6880 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus Ral GEF with PH domain and SH3 binding motif 2
            (Ralgps2), transcript variant 1, mRNA.
ACCESSION   NM_001401396 XM_039090751
VERSION     NM_001401396.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6880)
  AUTHORS   Ceriani,M., Amigoni,L., Scandiuzzi,C., Berruti,G. and Martegani,E.
  TITLE     The PH-PxxP domain of RalGPS2 promotes PC12 cells differentiation
            acting as a dominant negative for RalA GTPase activation
  JOURNAL   Neurosci Res 66 (3), 290-298 (2010)
   PUBMED   20025911
  REMARK    GeneRIF: Demonstrated that the PH-PxxP domain of RalGPS2 inhibits
            RalA activation inducing PC12 differentiation redirecting NGF
            signal to MAPK pathway, and that this domain is involved in the
            actin remodelling which is necessary for neurites elongation.
REFERENCE   2  (bases 1 to 6880)
  AUTHORS   Rebhun,J.F., Chen,H. and Quilliam,L.A.
  TITLE     Identification and characterization of a new family of guanine
            nucleotide exchange factors for the ras-related GTPase Ral
  JOURNAL   J Biol Chem 275 (18), 13406-13410 (2000)
   PUBMED   10747847
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000013.1.
            
            On Feb 10, 2022 this sequence version replaced XM_039090751.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643297.41595.1,
                                           SRR26643292.49607.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-119               JAXUCZ010000013.1  71606621-71606739   c
            120-259             JAXUCZ010000013.1  71575434-71575573   c
            260-364             JAXUCZ010000013.1  71568302-71568406   c
            365-415             JAXUCZ010000013.1  71567288-71567338   c
            416-499             JAXUCZ010000013.1  71549134-71549217   c
            500-589             JAXUCZ010000013.1  71548500-71548589   c
            590-682             JAXUCZ010000013.1  71547225-71547317   c
            683-809             JAXUCZ010000013.1  71542019-71542145   c
            810-947             JAXUCZ010000013.1  71507560-71507697   c
            948-1038            JAXUCZ010000013.1  71507353-71507443   c
            1039-1106           JAXUCZ010000013.1  71504712-71504779   c
            1107-1242           JAXUCZ010000013.1  71503227-71503362   c
            1243-1394           JAXUCZ010000013.1  71502512-71502663   c
            1395-1449           JAXUCZ010000013.1  71496929-71496983   c
            1450-1527           JAXUCZ010000013.1  71494346-71494423   c
            1528-1633           JAXUCZ010000013.1  71492870-71492975   c
            1634-1726           JAXUCZ010000013.1  71490601-71490693   c
            1727-1832           JAXUCZ010000013.1  71485038-71485143   c
            1833-1924           JAXUCZ010000013.1  71482905-71482996   c
            1925-6880           JAXUCZ010000013.1  71474835-71479790   c
FEATURES             Location/Qualifiers
     source          1..6880
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13q22"
     gene            1..6880
                     /gene="Ralgps2"
                     /note="Ral GEF with PH domain and SH3 binding motif 2"
                     /db_xref="GeneID:304887"
                     /db_xref="RGD:1311537"
     exon            1..119
                     /gene="Ralgps2"
                     /inference="alignment:Splign:2.1.0"
     exon            120..259
                     /gene="Ralgps2"
                     /inference="alignment:Splign:2.1.0"
     CDS             203..1954
                     /gene="Ralgps2"
                     /note="isoform 1 is encoded by transcript variant 1; Ral-A
                     exchange factor RalGPS2; ras-specific guanine
                     nucleotide-releasing factor RalGPS2"
                     /codon_start=1
                     /product="ras-specific guanine nucleotide-releasing factor
                     RalGPS2 isoform 1"
                     /protein_id="NP_001388325.1"
                     /db_xref="GeneID:304887"
                     /db_xref="RGD:1311537"
                     /translation="MDLMNGQASSVNIGATVSEKSSSSESLSEKGSELKKSFDAVVFD
                     VLKVTPEEYAGQITLMDVPVFKAIQPDELSSCGWNKKEKYSSAPNAVAFTRRFNHVSF
                     WVVREILHAQTLKIRAEVLSHYIKTAKKLYELNNLHALMAVVSGLQSAPIFRLTKTWA
                     LLSRKDKTTFEKLEYVMSKEDNYKRLRDYISSLKMTPCIPYLGIYLSDLTYIDSAYPS
                     TGSILENEQRSNLMNNILRIISDLQQSCEYDIPMLPHVQKYLNSVQYIEELQKFVEDD
                     NYKLSLKIEPGASTPRSAASREDLAGPDVGASPQGGRKSSAAEGALLPQTPPSPRHLI
                     PHGHRKCHSLGYNFIHKMNTAEFKSATFPNAGPRHLLDDSVMEPHAPSRGQAESSTLS
                     SGISIGSSDGSELSEETSWPAFERNRLYHSLGPVTRMPRNGYRSHMKASSSAESEDLA
                     VHLYPGAVTIQGVLRRKTLLKEGKKPTVASWTKYWAALCGTQLFYYPAKSLKATERKH
                     FKSTSNKNVSVVGWMVMMADDPEHPDLFLLTDSEKGNSYKFQAGSRMNAMLWFKHLSA
                     ACQSNRQQVPTNLMTFE"
     exon            260..364
                     /gene="Ralgps2"
                     /inference="alignment:Splign:2.1.0"
     exon            365..415
                     /gene="Ralgps2"
                     /inference="alignment:Splign:2.1.0"
     exon            416..499
                     /gene="Ralgps2"
                     /inference="alignment:Splign:2.1.0"
     exon            500..589
                     /gene="Ralgps2"
                     /inference="alignment:Splign:2.1.0"
     exon            590..682
                     /gene="Ralgps2"
                     /inference="alignment:Splign:2.1.0"
     exon            683..809
                     /gene="Ralgps2"
                     /inference="alignment:Splign:2.1.0"
     exon            810..947
                     /gene="Ralgps2"
                     /inference="alignment:Splign:2.1.0"
     exon            948..1038
                     /gene="Ralgps2"
                     /inference="alignment:Splign:2.1.0"
     exon            1039..1106
                     /gene="Ralgps2"
                     /inference="alignment:Splign:2.1.0"
     exon            1107..1242
                     /gene="Ralgps2"
                     /inference="alignment:Splign:2.1.0"
     exon            1243..1394
                     /gene="Ralgps2"
                     /inference="alignment:Splign:2.1.0"
     exon            1395..1449
                     /gene="Ralgps2"
                     /inference="alignment:Splign:2.1.0"
     exon            1450..1527
                     /gene="Ralgps2"
                     /inference="alignment:Splign:2.1.0"
     exon            1528..1633
                     /gene="Ralgps2"
                     /inference="alignment:Splign:2.1.0"
     exon            1634..1726
                     /gene="Ralgps2"
                     /inference="alignment:Splign:2.1.0"
     exon            1727..1832
                     /gene="Ralgps2"
                     /inference="alignment:Splign:2.1.0"
     exon            1833..1924
                     /gene="Ralgps2"
                     /inference="alignment:Splign:2.1.0"
     exon            1925..6880
                     /gene="Ralgps2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcactcccct cccctgagcg ccggcggcgg gcgctgaatg agagacgccg actgctctgc
       61 tgggcgagcg ctgcctcgga gctgcgggcg aagtggaggc gacagccgcg gctgcacagg
      121 aatgatgtat ttgtggcctt ggacatgaag taatcagacc tctgttgctg ttaacatagg
      181 ctcagggact gatgaggaaa gcatggacct aatgaacggg caggcaagca gtgttaatat
      241 cggggctaca gtttctgaga aaagtagcag ctctgagtca ttaagtgaga agggctctga
      301 attgaagaaa agctttgatg ctgtggtatt tgacgttctt aaggttacgc cagaagaata
      361 cgcgggtcag ataacactaa tggatgttcc agtgtttaaa gctattcagc cagatgaact
      421 ttcaagttgt ggatggaata aaaaagaaaa atacagttct gcaccaaatg cagttgcttt
      481 cacaagaaga tttaatcacg taagcttttg ggttgtaaga gagattctcc atgctcagac
      541 cctgaaaata agagcagaag ttttgagcca ctatatcaaa accgctaaga aactatatga
      601 actcaacaac cttcatgctc tcatggctgt ggtttctggc ttacagagtg cgccaatttt
      661 caggttgact aagacgtggg cgttattaag tcgaaaagac aaaactacct ttgaaaagtt
      721 agaatatgta atgagtaaag aagacaacta caaaagactc cgagactata taagtagctt
      781 aaagatgact ccttgcatcc cctacttagg tatctatttg tctgacttga cctacattga
      841 ctcagcgtac ccgtccacag gcagcattct agaaaatgag caaagatcaa atttgatgaa
      901 taacattctt cgaataattt ctgacttaca gcagtcctgt gaatatgata ttcccatgtt
      961 gcctcatgtc caaaaatacc tgaactctgt tcagtatata gaagaactac aaaagtttgt
     1021 ggaggacgat aattacaagc tctccttaaa gatagaacca ggagcgagta caccgcgctc
     1081 agctgcctcc agggaagatc tggcaggtcc tgacgtagga gcctcgccac aaggcgggag
     1141 gaagagcagt gctgccgagg gagccttact gccacagacg cccccgtccc ctcggcacct
     1201 cattccacat ggacacagga agtgccatag cttgggttac aatttcattc ataagatgaa
     1261 cacagcagaa tttaagagtg caacgttccc aaatgcaggg ccacggcacc tgctggatga
     1321 tagcgtcatg gagccacacg caccatcacg aggccaagcc gagagctcta cactttccag
     1381 tgggatatcc atagggagca gtgatggttc tgaactaagc gaagaaacct catggccagc
     1441 ttttgaaagg aacagattat accattctct cggcccggtg acaagaatgc cacgaaatgg
     1501 ctatcgaagc cacatgaagg ccagcagttc tgcagagtcg gaagacttgg cggtgcacct
     1561 gtacccaggg gctgttacta ttcaaggcgt tctcaggagg aaaactttgt taaaagaagg
     1621 caaaaaaccc acagtagcat cttggacaaa gtattgggca gccttgtgtg gaacacagct
     1681 tttttactat cctgccaaat ctctgaaggc tacagaaaga aagcacttca aatcaacgtc
     1741 aaataagaat gtgtctgtgg tgggctggat ggtgatgatg gccgatgatc cagagcatcc
     1801 agacctcttt ctgctgactg actccgagaa aggaaattca tacaagtttc aagctggcag
     1861 caggatgaat gcaatgctgt ggtttaaaca cttgagcgcg gcctgccaga gtaaccggca
     1921 acaggttcct acaaacttga tgacttttga gtaaaagcct gagaaagaag aggagcgctg
     1981 ttcccgtgta gagccaggac ctgccgagag ccccagccgc accatcccgt gccaggaaga
     2041 gcccccaggc tccagcccag cctccggagc tcagaaccaa aagcactaat ttcagggaat
     2101 gaagtgagat gttcccagag aaaaacaatg gagttgcaaa acaaactgcc atgaactacg
     2161 ctcacctccg aactgacctg tggaaaccac tgccttaaca gagtgcgagg agaaccgacg
     2221 ccaaatagtg tgtgtgcgcg tcttctggcg gtgctgtgct tggaataaat cgtagctaat
     2281 ttgcatttct tttaactttg tactaattct ggaggggaaa ccatcttttt tagatacact
     2341 ttaaaacgga aaaacatttc ttacggattc catcaggagc tgtttaggtg cagtgagaag
     2401 tccttgtgag gaacgggccc acagggtctg ctcagcctct gctagtggct ccctcactcc
     2461 tacagcagga cccaggagtg acacggtgcg ggagctgtgg gtgctgtctg ggctagggca
     2521 cactttactc tggatgaggg atgcgtttaa acactacagt ttggaattca gtgctagaag
     2581 gagaatatga ttcctgcagg aggtacaatg gccgagtgtt cacatgccag caggcacagt
     2641 aaacacggat gtttctaacc acggttaaag tttaactttc tgtatgaatt cattgttggg
     2701 cccaaatcca cctaggagca tggtgatccc gtgctgcggt ttctgtagcc ttttctcgag
     2761 tcaagtggat tctccagcca caggcatcat cgccacgggg aagaccctgg tgctccccgg
     2821 gtattgccct ttgcagtgtt taatgtgcag tatgccagac ttatttctta ttgaatttgt
     2881 ttttgttcag tatgaggttc aaaagcattc gtgtttaaaa gactcttcat gtatatgtat
     2941 gtattttatt gtaagacaaa acagttttaa gaagaataaa ggcaaagttt gcagttctag
     3001 gaattaaaaa tataaaacct tttctcgtat gacttcacac aatgtaccag aacatttttc
     3061 agaaagttat tctcgctccc caatctgaaa accatgtttt gttagtaact gaccaagttt
     3121 gagagagctg tcccaagcaa ttacacattt aacttttaaa aggaatgctg gatttgacat
     3181 agcttagaat tttaaccaga agtagcagtt gactgctgtg gggttggttc ccactttcat
     3241 agtggcccgt gtggaaaggc cctgactgat ggacacctcc tagcaaactg ctcactctgt
     3301 cagtctcatg tagtctcaca tttgtgtcct tcaagaactg aatacttaac cggaagtatt
     3361 ttatcaccac atccccggag cttcattaga aaatactgtc tgccgtaatt agctaaaaaa
     3421 gaagttagtc caagagaaag ggaagactga ggatttctga tttgaataat tcaaatatgt
     3481 ttgtcatgtg acagaaatac catatttggg gtttcattga caagcagagg aaggcaaatg
     3541 ttgccgattt ttccgataat ggggtctaag ttaacccagt tgggtatcca cagacagagt
     3601 ctgagtggcc ggctttcttt tctttttcta atcccttagt ttattttgat ccagagtaat
     3661 ttttaaataa tatgcacgcc gtctttgagg tactgtgaat gagaaccctg tggctgttgt
     3721 attactactt cgtcatgaaa aagctaaagc cgtcacattg taggtgatca cagtggtgaa
     3781 gataggtagc cagcagagtc cagcctcaca gagaaagggc gaaatggaag ctgtgttcag
     3841 tacatccagc tctggacact tatggatggg tagagaaaga accgaattca gatttgatta
     3901 gccaggctcc tggctgaaac taaaactttt tctaccttca gatttgctct aaggatcaac
     3961 cattgatttt taggatggtt ttagaacccg tgaggaggag atagaatagg tgaggtttat
     4021 ctgtagttca ccaaatagta ttagattata attgaagcaa agaaaacatt gccaaagtat
     4081 agtgaatatt tgagtatttt atttattgga gaatgaatcc tatcacgctc accctcggtt
     4141 gtaaattaat gattaactgg gtcatttccc atgaacatta aagcccgttt gcttctctca
     4201 tagccaagga gaacttttag agaaattcaa ttaaaatcta aactttataa acagactgaa
     4261 tggaatattg tatggcctgg tgcaatagtg tgtagcactg gccatatctc atctgacaat
     4321 gaattcaaac acagaggctt atttttctgt ctttaaagtg cacctgtgac agtgactgca
     4381 tgatgtcccc aggcactgct ttgggcttca tgtgcttgct tttcagaata ttgcaatatg
     4441 ctgtctaaaa ctgtaaggat cagaagactt gatggaatac atctttcagt ttggtttcat
     4501 tcagtgtgta cttgtgttaa cagttcaaaa agaatccaac ttgggactgt atttttctgt
     4561 cttcgtagac atgtttcagt ggcaattatt ttttctgtgt catcagtacc atctgccatt
     4621 gtgccccctt ctgcagtctc ttgctcttga catcgagtca gttacaatgt tcataactgc
     4681 agacttgttt tagtcttgga gatttataag aagcccaaca agcaacattg taatccagga
     4741 ggaatgtagc ctgcttagac gcctccctgc atggttgaag catgggtgag cacagcctca
     4801 tcagtctgtg gggctgcatt tacccatcca gatgacctca acataaagtc tttcttcatg
     4861 aagttaaacc tgtaaagttt ctgtgtttaa aactgaggca ttatattgat tggaaatcag
     4921 tgctaattag acttactaac tcattggctt tttagaataa cgtacccttc ccttttattg
     4981 atggacgttt ctttccagct gggtttaagc catcctatta ccttccacag acgagacttg
     5041 ctggtattgc ttcatggaag attggcttta atcaaaagaa atcctatgcc tccggcgctt
     5101 gtaaaagaat tggatccttt tgaaatggat gctgaagtgc actgtgctga ccaacagcgg
     5161 tgaacaccgc cactgctcag agggtgacaa agcaagcagt ggttggagcg tgtctgactt
     5221 gggcctcctt ttagggcttg tgttcatttt cttttatatc atttcttaga aatatagtac
     5281 catcctagtt ttgtagccag gagggtattg cttcttcaag cctctgaggt acaagaattg
     5341 ctattaaaca gtactgatga taaattccgt gcgaaccttt ccaaaatgat ttaagtacct
     5401 tttggatttg cttccctaaa aatggcttgt gtctaaattg acttttcaat ttgagaatct
     5461 actgaagaga tgtatagaca ccagttcctc agcccagtga aagcattgct ttttacctat
     5521 ctccagccgg agtgtgggga aaattgggtc agtatcttag caatttgaag gccagtttta
     5581 gggtattgag agtgactaaa accacctgcc ctcttaaccc tctgatgtga ggggcgtgct
     5641 gtccctctct ccacccacca aatctaaaca gtcgtaaaga agactctaga catctagata
     5701 ctcacagtcc tctgctctta gatcttaatt ctttttcaaa taagcttgcc tccctgttgc
     5761 tgtcctgtcc aggaatgtga gaaatagatc cactcagatc aaccttctga tgagctctca
     5821 atgttggctt tgaagagcct ccgtctagct gagtgtacct ttattttgtg tggttttgag
     5881 cttaggttta atgataagac ctgttgggga aaccctaaag gaataaaata cacacagaag
     5941 gagttgttga gattttctaa aataccatga tggccgtctg ttgagccaga aatgaaactt
     6001 atttcagttt cctacttaag acagaatgaa caagcagtca cttctcctgc ttgttactac
     6061 taacttgaac atatgcggga acggtgccac actaataata aatcaggggg agactcagcc
     6121 agggcagttt gaaaacattg tgcttaagac attgaaagtc caggagatac cgtcatctga
     6181 gtaggaatgc tgttttcagg gtccttcgtg aacagacata tacatacaca cagtctctgg
     6241 acacaaggaa gggctagctg ctccttgggg gctgttagca aaacttaaag tctgacagcc
     6301 agttggtctg caaaatagag ttaagggagt ccatggagag cttggggata ggaagacaga
     6361 aaacaccaaa tattagcaga atatttcaaa tgttcatgcc aagtaaaaaa atgtcaggtc
     6421 cctgaagatg tcacttgaaa gaactactca aaataggatt ctaagaagta ctaacttaac
     6481 aggtgtgaca aagggagata atgggacacg gaagtctcta gtgtttctgt accttgactg
     6541 ctgactctct tcattctcca acagcctact gaaggagctg gaaggaggaa ctcagacatc
     6601 ttagggataa ttacccaaaa ctaataatac aaaaaaaaaa aatcacaagc cattttattc
     6661 tctgttccag ttaacttgag gtacttaatg atgaagtatt tgcttgcact atgacctcct
     6721 cctcgggtga cgtgcaactt ggttcctctc tggttttgtt tctttttaat atgcagaagt
     6781 gagtgtgtga tcttcagtgt gtttgcataa gctaacttaa attgaattta agaacagttt
     6841 tctgaactat ttctattgaa ataaatgact taaaagtgta
//
