LOCUS       NM_001414180            2705 bp    mRNA    linear   ROD 18-FEB-2024
DEFINITION  Rattus norvegicus RAS like proto-oncogene A (Rala), transcript
            variant 2, mRNA.
ACCESSION   NM_001414180 XM_017600665
VERSION     NM_001414180.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Lemstrova,R., Brynychova,V., Hughes,D.J., Hlavac,V., Dvorak,P.,
            Doherty,J.E., Murray,H.A., Crockard,M., Oliverius,M., Hlavsa,J.,
            Honsova,E., Mazanec,J., Kala,Z., Lovecek,M., Havlik,R., Ehrmann,J.,
            Strouhal,O., Soucek,P., Melichar,B. and Mohelnikova-Duchonova,B.
  TITLE     Dysregulation of KRAS signaling in pancreatic cancer is not
            associated with KRAS mutations and outcome
  JOURNAL   Oncol Lett 14 (5), 5980-5988 (2017)
   PUBMED   29113235
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Nakamura,T., Yasuda,S., Nagai,H., Koinuma,S., Morishita,S.,
            Goto,A., Kinashi,T. and Wada,N.
  TITLE     Longest neurite-specific activation of Rap1B in hippocampal neurons
            contributes to polarity formation through RalA and Nore1A in
            addition to PI3-kinase
  JOURNAL   Genes Cells 18 (11), 1020-1031 (2013)
   PUBMED   24165023
  REMARK    GeneRIF: specific activation of Rap1B contributes to neuronal
            polarization via interaction with RalA and Nore1A in addition to
            PI3-kinase.
REFERENCE   3  (bases 1 to 2705)
  AUTHORS   Simicek,M., Lievens,S., Laga,M., Guzenko,D., Aushev,V.N., Kalev,P.,
            Baietti,M.F., Strelkov,S.V., Gevaert,K., Tavernier,J. and
            Sablina,A.A.
  TITLE     The deubiquitylase USP33 discriminates between RALB functions in
            autophagy and innate immune response
  JOURNAL   Nat Cell Biol 15 (10), 1220-1230 (2013)
   PUBMED   24056301
REFERENCE   4  (bases 1 to 2705)
  AUTHORS   Xie,L., Kang,Y., Liang,T., Dolai,S., Xie,H., Parsaud,L.,
            Lopez,J.A., He,Y., Chidambaram,S., Lam,P.P., James,D.E., Sugita,S.
            and Gaisano,H.Y.
  TITLE     RalA GTPase tethers insulin granules to L- and R-type calcium
            channels through binding alpha2 delta-1 subunit
  JOURNAL   Traffic 14 (4), 428-439 (2013)
   PUBMED   23346930
  REMARK    GeneRIF: RalA modulates insulin release by binding to auxiliary
            calcium channel subunits on the insulin granules.
REFERENCE   5  (bases 1 to 2705)
  AUTHORS   Peschard,P., McCarthy,A., Leblanc-Dominguez,V., Yeo,M.,
            Guichard,S., Stamp,G. and Marshall,C.J.
  TITLE     Genetic deletion of RALA and RALB small GTPases reveals redundant
            functions in development and tumorigenesis
  JOURNAL   Curr Biol 22 (21), 2063-2068 (2012)
   PUBMED   23063435
REFERENCE   6  (bases 1 to 2705)
  AUTHORS   Frankel,P., Aronheim,A., Kavanagh,E., Balda,M.S., Matter,K.,
            Bunney,T.D. and Marshall,C.J.
  TITLE     RalA interacts with ZONAB in a cell density-dependent manner and
            regulates its transcriptional activity
  JOURNAL   EMBO J 24 (1), 54-62 (2005)
   PUBMED   15592429
REFERENCE   7  (bases 1 to 2705)
  AUTHORS   Takaya,A., Ohba,Y., Kurokawa,K. and Matsuda,M.
  TITLE     RalA activation at nascent lamellipodia of epidermal growth
            factor-stimulated Cos7 cells and migrating Madin-Darby canine
            kidney cells
  JOURNAL   Mol Biol Cell 15 (6), 2549-2557 (2004)
   PUBMED   15034142
  REMARK    GeneRIF: RalA activation is required for the induction of
            lamellipodia, and conversely, lamellipodial protrusion seems to be
            required for the RalA activation, suggesting the presence of a
            positive feedback loop between RalA activation and lamellipodial
            protrusion
REFERENCE   8  (bases 1 to 2705)
  AUTHORS   Ohta,Y., Suzuki,N., Nakamura,S., Hartwig,J.H. and Stossel,T.P.
  TITLE     The small GTPase RalA targets filamin to induce filopodia
  JOURNAL   Proc Natl Acad Sci U S A 96 (5), 2122-2128 (1999)
   PUBMED   10051605
REFERENCE   9  (bases 1 to 2705)
  AUTHORS   Cantor,S.B., Urano,T. and Feig,L.A.
  TITLE     Identification and characterization of Ral-binding protein 1, a
            potential downstream target of Ral GTPases
  JOURNAL   Mol Cell Biol 15 (8), 4578-4584 (1995)
   PUBMED   7623849
REFERENCE   10 (bases 1 to 2705)
  AUTHORS   Wildey,G.M., Viggeswarapu,M., Rim,S. and Denker,J.K.
  TITLE     Isolation of cDNA clones and tissue expression of rat ral A and ral
            B GTP-binding proteins
  JOURNAL   Biochem Biophys Res Commun 194 (1), 552-559 (1993)
   PUBMED   7687439
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000293.1.
            
            [WARNING] On Apr 3, 2024 this sequence was replaced by
            NM_001414180.2.
            
            On Nov 16, 2022 this sequence version replaced XM_017600665.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR8487226.44444.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132283, SAMD00132289
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-193               JACYVU010000293.1  4093008-4093200
            194-260             JACYVU010000293.1  4109903-4109969
            261-407             JACYVU010000293.1  4131195-4131341
            408-616             JACYVU010000293.1  4134161-4134369
            617-791             JACYVU010000293.1  4138322-4138496
            792-2705            JACYVU010000293.1  4144124-4146037
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="17"
                     /map="17q11"
     gene            1..2705
                     /gene="Rala"
                     /note="RAS like proto-oncogene A"
                     /db_xref="GeneID:81757"
                     /db_xref="RGD:619851"
     exon            1..193
                     /gene="Rala"
                     /inference="alignment:Splign:2.1.0"
     exon            194..260
                     /gene="Rala"
                     /inference="alignment:Splign:2.1.0"
     exon            261..407
                     /gene="Rala"
                     /inference="alignment:Splign:2.1.0"
     CDS             294..914
                     /gene="Rala"
                     /EC_number="3.6.5.2"
                     /note="v-ral simian leukemia viral oncogene homolog A (ras
                     related); RALA Ras like proto-oncogene A"
                     /codon_start=1
                     /product="ras-related protein Ral-A"
                     /protein_id="NP_001401109.1"
                     /db_xref="GeneID:81757"
                     /db_xref="RGD:619851"
                     /translation="MAANKPKGQNSLALHKVIMVGSGGVGKSALTLQFMYDEFVEDYE
                     PTKADSYRKKVVLDGEEVQIDILDTAGQEDYAAIRDNYFRSGEGFLCVFSITEMESFA
                     ATADFREQILRVKEDENVPFLLVGNKSDLEDKRQVSVEEAKNRADQWNVNYVETSAKT
                     RANVDKVFFDLMREIRARKMEDSKEKNGKKKRKSLAKRIRERCCIL"
     exon            408..616
                     /gene="Rala"
                     /inference="alignment:Splign:2.1.0"
     exon            617..791
                     /gene="Rala"
                     /inference="alignment:Splign:2.1.0"
     exon            792..2705
                     /gene="Rala"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggctgctgca gagcggcccg gaggagggtg ggtctcccta gagcggagcg ccggagtcct
       61 ccccctccgc cgccgcccag gccgccgccg ccctcacgat tcctcctgcc gcgccccgcg
      121 cgggactccg agcgcggtcc gatcggcaca gccgaggctc aagagcccgc ggggcgagcg
      181 gctcgcggtg caggaaccat ctctgagcaa caagcttgtg gcaccttgat attaggctta
      241 ctgctacaaa tatacaaaat attcttttca gtcctctgaa gactgcacac aggatggctg
      301 caaacaagcc caagggtcag aattctttgg ccttacacaa agtcatcatg gtgggcagtg
      361 gtggtgtggg caagtctgct ctgactctgc agttcatgta tgatgagttt gtagaagact
      421 atgaacctac caaagcagac agctacagga agaaggtagt gctggatggg gaggaagtgc
      481 agatcgacat cttagataca gcagggcagg aagactacgc tgcaattaga gacaactact
      541 tccgaagtgg ggagggattc ctctgtgtct tctctatcac agagatggag tcctttgcag
      601 ctacagcgga cttcagggaa cagattttaa gagtaaaaga agatgagaat gtcccatttc
      661 tcctggttgg taacaaatca gatttagaag ataaaaggca ggtttctgta gaagaggcaa
      721 aaaacagagc tgaccagtgg aacgttaact atgtggagac gtctgctaaa acgcgcgcca
      781 acgttgacaa ggtatttttt gatttaatga gggaaatacg agccagaaag atggaagaca
      841 gcaaagaaaa aaatggaaaa aagaagagga aaagtttagc caagagaatc agagaaagat
      901 gctgcatttt ataatcaaag cccaaactcc tttcttatcc tgacctgacc atactaataa
      961 atataattta taagcattgc cattggaggc tcaattgact gaaattactt taacattttg
     1021 gaaattgtag tgtatcacta aaagcatgaa ttggaactgc actggaagtc aaattcactt
     1081 aaaaaagaaa ttgtgtggct tcaccaagaa gcagagttca acttatttca taatcgccta
     1141 cctttatccc agtcctgaat gtagcgtgta agcttggttg ggttttttgg ggaggtgggg
     1201 ggggggggca gtctttctca aactgttaat caagaggtga aatggggtgg gggggtggga
     1261 ggactggcca atttcctctg gtgtttatta taaagcttaa attttatatt attttaaaat
     1321 gtcttggtct tctactgcct tgaaaaatga caattgtgaa cacgatagtt aactgtacca
     1381 ctttttaaac tgttgttatg caaatcttga agaagttact ggcatggttg ttacatatag
     1441 taaaactgag agtaattcat ctgtgaatct gcatattaat tacctgctga ttgacttaga
     1501 aaaaagatgt gaacttatac atggacattt ttgaatatgc cttaatttag aaactgaaaa
     1561 acatccagtt acatcattgt gggtgtgttc tcactagcac tcgctgccca tgtgactaat
     1621 aacagaaggt tttatcttgc caggcagtgt agagctacaa gaaagattcc tgagaaaagt
     1681 ccaactgcta ggagtctgac caagttaaag atgtgtgcca tactctggtt ctgagagttc
     1741 cagagctcct tcattggttt taataaacta taaggtagtt aaagatgaaa ggccagcata
     1801 tatttataca atgagtttag attatgggtg tgagtaccaa ttaagtgaat gctacttttg
     1861 tgtagagtgc tggtctccag gagtcgcttt ctgcaagtga tgtgcattta agtgtttaat
     1921 ctcctgggag cagggcatag ggaagcctct caggagagct aagtggtctg tactaggaca
     1981 cgaggaaata atgcttgtca gttctaaatg tgtactcctg aggttacagt ttgggtgtta
     2041 ggagaagtct ttgcagtctc tttatgttta taatgaatac tgtatcaata tttcctaaca
     2101 tgaagcagtc aaacatgtat ggcacatact gaccagatgt cgggctgtga tgtttccgct
     2161 gcaagaattc aagcatgctg ttaggagcat aatttcaatg ggaagtaaac cactattaga
     2221 tttttctttt cctctgtggg gctaagacac ttgattaaac agtttttctt gttgcacagt
     2281 cctaatttcc tatggtcaag gagacactca ggcattccag gaaacaaatg tgttcatgac
     2341 gttaccggag acaggcattc aggaactctg ctgtctagtt actatattca gcttatcata
     2401 agtatttgtc cttgaagggt ttgatttaat attttcacag actaggccat gtgcaaaatt
     2461 agagttccct gtgtaacccc agccagcagg cccagcatgg ctgtgagcac tcattacagt
     2521 cttgttgacc aggttcctaa ccactaacat tgcaattagc tttgagacct tccctctcct
     2581 gggtactagg tgctatgaag ccagctgaca gagaagcatc acatgtcttg ggctgatgcc
     2641 gctacccggt tcatttactt gcaatttgag ccatttaaag accaataaac ttccttttct
     2701 aaaaa
//
