LOCUS       NM_001100528            3546 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus recombination activating 2 (Rag2), mRNA.
ACCESSION   NM_001100528 XM_001079235 XM_230374
VERSION     NM_001100528.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Noto,F.K., Adjan-Steffey,V., Tong,M., Ravichandran,K., Zhang,W.,
            Arey,A., McClain,C.B., Ostertag,E., Mazhar,S., Sangodkar,J.,
            DiFeo,A., Crawford,J., Narla,G. and Jamling,T.Y.
  TITLE     Sprague Dawley Rag2-Null Rats Created from Engineered
            Spermatogonial Stem Cells Are Immunodeficient and Permissive to
            Human Xenografts
  JOURNAL   Mol Cancer Ther 17 (11), 2481-2489 (2018)
   PUBMED   30206106
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Patel,D.J. and Wang,Z.
  TITLE     Readout of epigenetic modifications
  JOURNAL   Annu Rev Biochem 82, 81-118 (2013)
   PUBMED   23642229
  REMARK    Review article
REFERENCE   3  (bases 1 to 3546)
  AUTHORS   Hewitt,S.L., Yin,B., Ji,Y., Chaumeil,J., Marszalek,K.,
            Tenthorey,J., Salvagiotto,G., Steinel,N., Ramsey,L.B., Ghysdael,J.,
            Farrar,M.A., Sleckman,B.P., Schatz,D.G., Busslinger,M.,
            Bassing,C.H. and Skok,J.A.
  TITLE     RAG-1 and ATM coordinate monoallelic recombination and nuclear
            positioning of immunoglobulin loci
  JOURNAL   Nat Immunol 10 (6), 655-664 (2009)
   PUBMED   19448632
  REMARK    Erratum:[Nat Immunol. 2009 Jun;10(6). doi: 10.1038/ni.1735]
REFERENCE   4  (bases 1 to 3546)
  AUTHORS   Matthews,A.G., Kuo,A.J., Ramon-Maiques,S., Han,S., Champagne,K.S.,
            Ivanov,D., Gallardo,M., Carney,D., Cheung,P., Ciccone,D.N.,
            Walter,K.L., Utz,P.J., Shi,Y., Kutateladze,T.G., Yang,W., Gozani,O.
            and Oettinger,M.A.
  TITLE     RAG2 PHD finger couples histone H3 lysine 4 trimethylation with
            V(D)J recombination
  JOURNAL   Nature 450 (7172), 1106-1110 (2007)
   PUBMED   18033247
REFERENCE   5  (bases 1 to 3546)
  AUTHORS   Ramon-Maiques,S., Kuo,A.J., Carney,D., Matthews,A.G.,
            Oettinger,M.A., Gozani,O. and Yang,W.
  TITLE     The plant homeodomain finger of RAG2 recognizes histone H3
            methylated at both lysine-4 and arginine-2
  JOURNAL   Proc Natl Acad Sci U S A 104 (48), 18993-18998 (2007)
   PUBMED   18025461
REFERENCE   6  (bases 1 to 3546)
  AUTHORS   Schwarz,K., Gauss,G.H., Ludwig,L., Pannicke,U., Li,Z., Lindner,D.,
            Friedrich,W., Seger,R.A., Hansen-Hagge,T.E., Desiderio,S.,
            Lieber,M.R. and Bartram,C.R.
  TITLE     RAG mutations in human B cell-negative SCID
  JOURNAL   Science 274 (5284), 97-99 (1996)
   PUBMED   8810255
REFERENCE   7  (bases 1 to 3546)
  AUTHORS   Li,T., Ramirez,K. and Palacios,R.
  TITLE     Distinct patterns of Fas cell surface expression during development
            of T- or B-lymphocyte lineages in normal, scid, and mutant mice
            lacking or overexpressing p53, bcl-2, or rag-2 genes
  JOURNAL   Cell Growth Differ 7 (1), 107-114 (1996)
   PUBMED   8788039
REFERENCE   8  (bases 1 to 3546)
  AUTHORS   Mombaerts,P., Iacomini,J., Johnson,R.S., Herrup,K., Tonegawa,S. and
            Papaioannou,V.E.
  TITLE     RAG-1-deficient mice have no mature B and T lymphocytes
  JOURNAL   Cell 68 (5), 869-877 (1992)
   PUBMED   1547488
REFERENCE   9  (bases 1 to 3546)
  AUTHORS   Shinkai,Y., Rathbun,G., Lam,K.P., Oltz,E.M., Stewart,V.,
            Mendelsohn,M., Charron,J., Datta,M., Young,F., Stall,A.M. et al.
  TITLE     RAG-2-deficient mice lack mature lymphocytes owing to inability to
            initiate V(D)J rearrangement
  JOURNAL   Cell 68 (5), 855-867 (1992)
   PUBMED   1547487
REFERENCE   10 (bases 1 to 3546)
  AUTHORS   Oettinger,M.A., Schatz,D.G., Gorka,C. and Baltimore,D.
  TITLE     RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J
            recombination
  JOURNAL   Science 248 (4962), 1517-1523 (1990)
   PUBMED   2360047
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            On Nov 27, 2020 this sequence version replaced NM_001100528.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643292.13453.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN06680412, SAMN06680413
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-131               JAXUCZ010000003.1  108357399-108357529
            132-190             JAXUCZ010000003.1  108360716-108360774
            191-3546            JAXUCZ010000003.1  108361898-108365253
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q31"
     gene            1..3546
                     /gene="Rag2"
                     /note="recombination activating 2"
                     /db_xref="GeneID:295953"
                     /db_xref="RGD:1305588"
     exon            1..131
                     /gene="Rag2"
                     /inference="alignment:Splign:2.1.0"
     exon            132..190
                     /gene="Rag2"
                     /inference="alignment:Splign:2.1.0"
     exon            191..3546
                     /gene="Rag2"
                     /inference="alignment:Splign:2.1.0"
     CDS             217..1800
                     /gene="Rag2"
                     /note="recombination activating protein 2; recombination
                     activating gene 2"
                     /codon_start=1
                     /product="V(D)J recombination-activating protein 2"
                     /protein_id="NP_001093998.1"
                     /db_xref="GeneID:295953"
                     /db_xref="RGD:1305588"
                     /translation="MSLQMVTVGHNIALIQPGFSLMNFDGQVFFFGQKGWPKRSCPTG
                     VFHFDIKQNHLKLKPAVFSKDSCYLPPLRYPATCSYKGSTDSDKHQYIIHGGKTPNNE
                     LSDKIYIMSVACKNNKKVTFRCTEKDLVGDVPEARYGHSIDVVYSRGKSVGVLFGGRS
                     YMPSTQRTTEKWNSVADCLPHVFLVDFEFGCATSYILPELQDGLSFHVSIARNDTIYI
                     LGGHSLASNIRPANLYRIRVDLPLGTPAVNCTVLPGGISVSSAIVTQTNNDEFVIVGG
                     YQLENQKRMVCSIVSLGDNRIEISEMETPDWTPDIKHSKIWFGSNMGNGTVFLGIPGD
                     NKQAMSEAFYFYMLRCSEDNSSEEQKIVSNSQTSTEDAGDSTPFEDSEEFCFSAEAIS
                     FDGDDEFDTYNEDDEDDESVTGYWITCSPTCDVDINTWVPFYSTELNKPAMIYCSHGD
                     GHWVHAQCMDLEERTLIHLSEGSNKYYCNEHVRIARALQTPKRNLPLQKPPMKSLHKK
                     GSGKVLTPAKKSFLRRLFD"
ORIGIN      
        1 cactctaccc ttcagccttc agcttgccac agtcaaacta aacagtcagt gaccctcccc
       61 aagtgccgag tttaattccc cgcttggcag caaggattca gagagggata agcagcccct
      121 ctggccttca gtgccaaaat aagagtactt cacatccaca agcaggaagt agacttcata
      181 cctctctaag ataaaagacc tactcacaat caaaaaatgt ccctgcagat ggttacagtg
      241 ggtcataaca tagccttaat tcaaccaggc ttctcactga tgaattttga tggtcaagtt
      301 tttttctttg gccaaaaagg ctggcctaag agatcctgcc ctactggagt ctttcatttt
      361 gatataaaac aaaatcatct caaactgaag cctgcagttt tctctaaaga ttcctgctac
      421 ctcccacctc ttcgttaccc agctacttgc tcgtacaaag gcagcacaga ctctgacaag
      481 catcaatata tcatccacgg agggaaaacg ccaaacaatg agctttccga taagatttat
      541 atcatgtctg tcgcttgcaa gaataacaaa aaagttactt tccgttgtac agagaaagac
      601 ttagtaggag atgtccctga agccagatat ggccattcca ttgacgtggt atatagccga
      661 ggaaaaagtg ttggtgttct ctttggagga cggtcataca tgccttctac ccaaagaacc
      721 acagaaaaat ggaatagtgt agctgattgc ctaccccatg ttttcttggt agattttgaa
      781 tttgggtgtg caacgtcata tattctccca gaacttcagg acgggctgtc ttttcatgtt
      841 tctattgcca gaaatgatac catttatatt ttgggaggac actcacttgc cagtaacata
      901 cgtcctgcta acttgtatag aataagagta gatcttcccc tgggtacccc agcagtgaat
      961 tgcactgtct tgccaggagg aatctctgtc tccagtgcaa ttgtcactca aacaaataat
     1021 gatgaatttg ttattgttgg tggttatcag ctggaaaatc aaaaaaggat ggtgtgtagc
     1081 attgtctctc taggggacaa caggattgaa attagtgaga tggagacccc agactggacc
     1141 ccagatatta agcatagcaa aatatggttt ggaagcaaca tgggaaacgg gactgttttc
     1201 cttggcatac caggagacaa caagcaggct atgtcagaag cattctattt ctatatgttg
     1261 agatgctctg aagataattc gagtgaggag cagaaaattg tctcaaacag tcagacatca
     1321 actgaagatg ccggggactc cactcccttt gaagactcgg aagaattttg tttcagtgct
     1381 gaagcaatca gctttgatgg tgatgatgaa tttgacactt acaatgaaga tgatgaagat
     1441 gatgaatctg taaccggcta ctggataacg tgttccccta cttgtgatgt ggacatcaat
     1501 acttgggttc cattctattc aactgagctc aataaacccg ccatgatcta ttgttctcat
     1561 ggggatggac actgggtgca tgcccagtgc atggatctgg aagaacgcac actcatccac
     1621 ttgtcagaag gaagcaacaa atattactgc aatgagcatg tgcggatagc aagagcatta
     1681 caaactccca aaagaaacct ccccttacaa aagcccccaa tgaaatccct ccacaaaaaa
     1741 ggctccggga aagtcttgac tcctgccaag aaatcctttc ttaggaggtt gtttgattag
     1801 ttcagcaaaa gcccctcaga ttcacgtgcg ttggtttcta aatctactct ttaaatcata
     1861 aacggaattt cgatttttgt ttactgaaat ctatatgcta cgttttggtt gtgtgaatta
     1921 agcgctgttt tgatgtgatg ttaagtacaa ttattctaac atgtgtggtt tttaacattt
     1981 tatctgtaat ttcttaaatg agaaatgtta cacagtcttt cattaaggat agcagtgata
     2041 aagtatagag ctcttgcatt attttgtaac aatctacatg ttgaatagta actaaatacc
     2101 aataaactaa tgcacaaaaa caattaagtt gttttgtgta ataaatagcc tatagttggt
     2161 ttaaacagtt aaaactatat tccacactac tgctgtttat aaattttaag gtggcctctt
     2221 ggattatacc catgaacaga cagatataga tatagatata gatatagata tagatataga
     2281 tatagatata gatatagata tagatataga tatagataga tagatataga tagatataga
     2341 tagacagaca gacagacaga cagatagata gagatagaga tagatatatt gtgtattcag
     2401 caccatgaag aaaaaaacta atctatatca agccaggtat agtgatggtg atacacgcct
     2461 gtaatcctgg caaatttgtg agtttgatgc tcgttgaggt atggtctctt gctatgtgtt
     2521 gtaatgctaa gtgcgggccc caagatctgc ttgctccaga gtcaagagag cccacaaata
     2581 gacccaagtg attctctgca accttgcccc caagttattt gtggttgagt gaataaagat
     2641 gccaacagcc aatagctgag cagaagatac ataggtgggg gttgatgatg ccctggcttg
     2701 gggtaggagg agaccatgag gaagggggag aaaaaggtga agtctcctcc ttcacctaaa
     2761 aaagcatagg ttaggagtca aaaaaacatg gccctgagtg ctggccactg ggagttaaga
     2821 gcagcccaga tgaaatgtcg caataacttg gggtataagt aggaaataga ttttaatagc
     2881 atataaggta gatatctacc cagccctaga gctaattaag gcttattgta aatataaagg
     2941 tcttacgtgt caggaaaaaa atggtcaaac aaggagtaga aactctgaat tgggatcaaa
     3001 attttctaca acagacaccg tctggggcta cattttatct caaatccaaa agctgataga
     3061 agacagagca ctggtggcat tgtcacgtcc catgctcaaa gtcctgagtc caatactgtg
     3121 gacattttct tgaattacct acatacgctt caggagtaaa aaatatattc caaacacacg
     3181 cctttgtccg aatcactcgc tttggggcct cacatttaat tccttcaaaa tcaatggttc
     3241 accccctttg aaaatgctca attgttgttc ctctttttcc ttaaagatgg ttgtcatctc
     3301 tggaaatagt catatgtcat tcaaggcccg atagcatgaa gccctgaagt gttgccttac
     3361 cactttaatt gaaaagtgaa atatgaccat aagcttagac agtggtttgg ggctattaat
     3421 ctcatggcag ggtttggggg tgcctaatac tgagtcctga actgttttct aagtacctgt
     3481 tttcaaacaa gtatggaaat gttatcaata aatatgctat caataaaaag aaaactttca
     3541 tattcc
//
