LOCUS       NM_017317               2704 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Rattus norvegicus RAB27A, member RAS oncogene family (Rab27a),
            mRNA.
ACCESSION   NM_017317
VERSION     NM_017317.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Zhang,J., Zhang,K., Qi,L., Hu,Q., Shen,Z., Liu,B., Deng,J.,
            Zhang,C. and Zhang,Y.
  TITLE     DENN domain-containing protein FAM45A regulates the homeostasis of
            late/multivesicular endosomes
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1866 (5), 916-929 (2019)
   PUBMED   30771381
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Booth,A.E., Tarafder,A.K., Hume,A.N., Recchi,C. and Seabra,M.C.
  TITLE     A role for Na+,K+-ATPase alpha1 in regulating Rab27a localisation
            on melanosomes
  JOURNAL   PLoS One 9 (7), e102851 (2014)
   PUBMED   25051489
  REMARK    GeneRIF: Data show that the intracellular M4M5 loop of human alpha1
            subunit of Na+,K+-ATPase (ATP1a1) interacts directly with rat rab
            GTP-binding protein Rab27a.
            Erratum:[PLoS One. 2016 Feb 03;11(2):e0148719. doi:
            10.1371/journal.pone.0148719.. PMID: 26840778]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2704)
  AUTHORS   Kawakami,T., He,J., Morita,H., Yokoyama,K., Kaji,H., Tanaka,C.,
            Suemori,S., Tohyama,K. and Tohyama,Y.
  TITLE     Rab27a is essential for the formation of neutrophil extracellular
            traps (NETs) in neutrophil-like differentiated HL60 cells
  JOURNAL   PLoS One 9 (1), e84704 (2014)
   PUBMED   24404184
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2704)
  AUTHORS   Imai,A., Ishida,M., Fukuda,M., Nashida,T. and Shimomura,H.
  TITLE     MADD/DENN/Rab3GEP functions as a guanine nucleotide exchange factor
            for Rab27 during granule exocytosis of rat parotid acinar cells
  JOURNAL   Arch Biochem Biophys 536 (1), 31-37 (2013)
   PUBMED   23702376
  REMARK    GeneRIF: These findings indicated that Madd functions as a guanine
            nucleotide exchange factor for Rab27 in parotid acinar cells and
            regulates isoproterenol-stimulated amylase release.
REFERENCE   5  (bases 1 to 2704)
  AUTHORS   Zheng,Y., Campbell,E.C., Lucocq,J., Riches,A. and Powis,S.J.
  TITLE     Monitoring the Rab27 associated exosome pathway using nanoparticle
            tracking analysis
  JOURNAL   Exp Cell Res 319 (12), 1706-1713 (2013)
   PUBMED   23092844
REFERENCE   6  (bases 1 to 2704)
  AUTHORS   Wilson,S.M., Yip,R., Swing,D.A., O'Sullivan,T.N., Zhang,Y.,
            Novak,E.K., Swank,R.T., Russell,L.B., Copeland,N.G. and
            Jenkins,N.A.
  TITLE     A mutation in Rab27a causes the vesicle transport defects observed
            in ashen mice
  JOURNAL   Proc Natl Acad Sci U S A 97 (14), 7933-7938 (2000)
   PUBMED   10859366
REFERENCE   7  (bases 1 to 2704)
  AUTHORS   Chen,D., Guo,J., Miki,T., Tachibana,M. and Gahl,W.A.
  TITLE     Molecular cloning and characterization of rab27a and rab27b, novel
            human rab proteins shared by melanocytes and platelets
  JOURNAL   Biochem Mol Med 60 (1), 27-37 (1997)
   PUBMED   9066979
REFERENCE   8  (bases 1 to 2704)
  AUTHORS   Nagata,K., Suzuki,T., Shibagaki,Y., Mizumoto,K., Okano,Y.,
            Kaziro,Y. and Nozawa,Y.
  TITLE     Characterization and site-directed mutagenesis of a low M(r)
            GTP-binding protein, ram p25, expressed in Escherichia coli
  JOURNAL   J Biol Chem 267 (27), 19600-19606 (1992)
   PUBMED   1527078
REFERENCE   9  (bases 1 to 2704)
  AUTHORS   Nagata,K., Satoh,T., Itoh,H., Kozasa,T., Okano,Y., Doi,T.,
            Kaziro,Y. and Nozawa,Y.
  TITLE     The ram: a novel low molecular weight GTP-binding protein cDNA from
            a rat megakaryocyte library
  JOURNAL   FEBS Lett 275 (1-2), 29-32 (1990)
   PUBMED   2124544
REFERENCE   10 (bases 1 to 2704)
  AUTHORS   Moore,K.J., Swing,D.A., Copeland,N.G. and Jenkins,N.A.
  TITLE     Interaction of the murine dilute suppressor gene (dsu) with
            fourteen coat color mutations
  JOURNAL   Genetics 125 (2), 421-430 (1990)
   PUBMED   2379821
  REMARK    Erratum:[Genetics 1990 Sep;126(1):285]
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC098667.1.
            
            On Jul 13, 2005 this sequence version replaced NM_017317.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC098667.1, EV762225.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q24"
     gene            1..2704
                     /gene="Rab27a"
                     /gene_synonym="ram"
                     /note="RAB27A, member RAS oncogene family"
                     /db_xref="GeneID:50645"
                     /db_xref="RGD:620918"
     exon            1..42
                     /gene="Rab27a"
                     /gene_synonym="ram"
                     /inference="alignment:Splign:2.1.0"
     exon            43..217
                     /gene="Rab27a"
                     /gene_synonym="ram"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..730
                     /gene="Rab27a"
                     /gene_synonym="ram"
                     /EC_number="3.6.5.2"
                     /note="rab-27; ram p25; GTP-binding protein Ram; low Mr
                     GTP-binding protein"
                     /codon_start=1
                     /product="ras-related protein Rab-27A"
                     /protein_id="NP_059013.1"
                     /db_xref="GeneID:50645"
                     /db_xref="RGD:620918"
                     /translation="MSDGDYDYLIKFLALGDSGVGKTSVLYQYTDGKFNSKFITTVGI
                     DFREKRVVYRANGPDGTVGRGQRIHLQLWDTAGQERFRSLTTAFFRDAMGFLLLFDLT
                     NEQSFLNVRNWISQLQMHAYCENPDIVLCGNKSDLEDQRAVKEEEARELAEKYGIPYF
                     ETSAANGTNISQAIEMLLDLIMKRMERCVDKSWIPEGVVRSNGHTSTDQLSEEKEKGL
                     CGC"
     exon            218..303
                     /gene="Rab27a"
                     /gene_synonym="ram"
                     /inference="alignment:Splign:2.1.0"
     exon            304..407
                     /gene="Rab27a"
                     /gene_synonym="ram"
                     /inference="alignment:Splign:2.1.0"
     exon            408..531
                     /gene="Rab27a"
                     /gene_synonym="ram"
                     /inference="alignment:Splign:2.1.0"
     exon            532..2677
                     /gene="Rab27a"
                     /gene_synonym="ram"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgcaacaccg cggggacagc acccgacaga gcaccaaacc gggtaaagca gagaggcctc
       61 catcatgtcg gatggagatt atgactacct catcaagttt ttggccttgg gagactctgg
      121 agtggggaag accagtgtac tgtaccagta cactgatggg aagttcaact ccaaattcat
      181 caccacagtg ggcattgatt tcagggaaaa gagagtggtg tacagagcga atggaccaga
      241 tggaactgta ggccgaggcc agagaattca cctgcagtta tgggacacgg cggggcagga
      301 gaggtttcgt agcctgacca cggcattctt cagggacgct atgggtttcc tgcttctgtt
      361 cgacctgaca aacgagcaaa gtttcctcaa tgtccgaaac tggataagcc agctacagat
      421 gcacgcgtac tgcgaaaacc cagatatagt gctctgcgga aataagagtg acctagaaga
      481 ccagagggct gtgaaagagg aggaagccag ggaactcgca gagaagtatg gaatccccta
      541 ttttgaaacc agcgctgcca atgggacaaa cataagccaa gcaattgaga tgctcctgga
      601 cctgatcatg aagcggatgg agcggtgtgt ggacaaatcc tggattccag agggggtggt
      661 gcggtccaat ggccatactt ctacagatca actcagtgag gagaaggaga aggggttatg
      721 cggctgttga gacaccgagt gagcatggtg gcggcagtgg cggcagcagc gcctggcccc
      781 tgcctgccaa atcctctgta gagaccaagc cagcatcacg tgccaagtgc tgctccttat
      841 ccccagtaga actgactcaa catccaattg taactcattg caacttcata tgatttagtc
      901 catgaattgg gggaactatt tcacagagcc aaaagtgtct tagagaaaaa aaaagcccat
      961 ggccgcgggt cattcaggga tccaagattt cagccatgga agattgcgtg tcatgtagaa
     1021 tgctgatggt cccgtcgcct gccttaacag tcaactgcct caacacagcc ctagagtctc
     1081 actgaagacg ttaagatgtt tgggcaatga aaagctagca tggtctagag aatccagagt
     1141 tcagaatcca taccgtagaa tatcactctc acgtgaatgc taataagtgt gtaccatgga
     1201 tcaatgtctt tcagcattac cttaaactta gccttccctt tgacatgaac gtaaacagat
     1261 ggcggccata tctataggct ctgagtagac cggattgact caccgtgagt ttgggggtgg
     1321 gtgaagtagg gatcaagttt agtcagattg tctggctttt agccgcgggg tcatggagac
     1381 cctcgagttt atccgtggtt cagcccacat ctgaacattg aagatgcatc tgctgtgtgt
     1441 gcatttgtac gtatgtctgg tctggcactt caggctgtgc tttcacttgt ctgtagactg
     1501 cgaatttgac ttttccatca agctttggtg gtgttggggt ttcccagttt gttttctccc
     1561 ccatttgtgt aactgaagga agctcttgtt aattatacag cttgtttatt tgctatctgg
     1621 agggagatca atgtctgtct ttaagcagtc acatttatat gctagtttag gagaggtgtt
     1681 ttttagcctg taactttcac tgagaatttg ggcacttatg ttttttgaca gtataaagga
     1741 aaccacgtgc tttgcatggt agttttcgag tggctttttt tgttgttgtt gtttttgttt
     1801 tgtttttttg gggggacagg gtcttattat gtagtttctg gccagcctgg aagtcccttt
     1861 gtagaccagg ctggcctcaa actcacagag atccacctgt ttcagccccc taagtgttgg
     1921 gataaaaggt gtggtcatca cacctggctc tcagaagatt tttaaaaatg gaacatcgat
     1981 catttattgc cattgtcctt tgaccccctc cccagacaaa atagccatgg ctactaagct
     2041 ttttctgtcc ctgaaataag ggactctttg aaacaccaaa gttgtcaccg aggcctatgc
     2101 atctgaatcc taaagaccgg aaacttgggg cccatcacag tcgtgccttc caatgttgct
     2161 ctaagacttc agtggactgt actgcacgtc agggcacgtt tcttaattaa tgtctccttt
     2221 gtaaatacaa acgtattact atgccaggtg ttggtggcgc actccttcaa tctcccgcag
     2281 ttgggaggca gaggcaggca gcgagtttga agccaggctg gtcaatagag tttcaggaca
     2341 gacagtgaca cagtgaaatt ctgtttcaag aatcaaagcc aatgtttgca cagtgcaggt
     2401 ttctagaagg agatccattg tcggattcgc cttctgttgg ccccgcacca ggcacggatg
     2461 ttaagatatg tgatagttgc ccagcgagag agcggccttg aattgttgaa atcaaacaaa
     2521 actgtattct ctttgaaaat gatttaaatt tgatataaaa taactgtact tattgtcact
     2581 gtatttttct atttacaaat ctgtatgaaa gcaaacgctc ttcagtgaac tttgtgccaa
     2641 ttaaaccata ataataaata ataaaagagg tccagtaaaa aaaaaaaaaa aaaaaaaaaa
     2701 aaaa
//
