LOCUS       NM_001105857            1976 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus pyrroline-5-carboxylate reductase 1 (Pycr1),
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001105857 XM_001081808 XM_039085691 XM_221200
VERSION     NM_001105857.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Yasuda,T., Kaji,Y., Agatsuma,T., Niki,T., Arisawa,M., Shuto,S.,
            Ariga,H. and Iguchi-Ariga,S.M.
  TITLE     DJ-1 cooperates with PYCR1 in cell protection against oxidative
            stress
  JOURNAL   Biochem Biophys Res Commun 436 (2), 289-294 (2013)
   PUBMED   23743200
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   De Ingeniis,J., Ratnikov,B., Richardson,A.D., Scott,D.A.,
            Aza-Blanc,P., De,S.K., Kazanov,M., Pellecchia,M., Ronai,Z.,
            Osterman,A.L. and Smith,J.W.
  TITLE     Functional specialization in proline biosynthesis of melanoma
  JOURNAL   PLoS One 7 (9), e45190 (2012)
   PUBMED   23024808
REFERENCE   3  (bases 1 to 1976)
  AUTHORS   Reversade,B., Escande-Beillard,N., Dimopoulou,A., Fischer,B.,
            Chng,S.C., Li,Y., Shboul,M., Tham,P.Y., Kayserili,H., Al-Gazali,L.,
            Shahwan,M., Brancati,F., Lee,H., O'Connor,B.D., Schmidt-von
            Kegler,M., Merriman,B., Nelson,S.F., Masri,A., Alkazaleh,F.,
            Guerra,D., Ferrari,P., Nanda,A., Rajab,A., Markie,D., Gray,M.,
            Nelson,J., Grix,A., Sommer,A., Savarirayan,R., Janecke,A.R.,
            Steichen,E., Sillence,D., Hausser,I., Budde,B., Nurnberg,G.,
            Nurnberg,P., Seemann,P., Kunkel,D., Zambruno,G., Dallapiccola,B.,
            Schuelke,M., Robertson,S., Hamamy,H., Wollnik,B., Van Maldergem,L.,
            Mundlos,S. and Kornak,U.
  TITLE     Mutations in PYCR1 cause cutis laxa with progeroid features
  JOURNAL   Nat Genet 41 (9), 1016-1021 (2009)
   PUBMED   19648921
  REMARK    Erratum:[Nat Genet. 2022 Feb;54(2):213. PMID: 35064218]
REFERENCE   4  (bases 1 to 1976)
  AUTHORS   Meng,Z., Lou,Z., Liu,Z., Li,M., Zhao,X., Bartlam,M. and Rao,Z.
  TITLE     Crystal structure of human pyrroline-5-carboxylate reductase
  JOURNAL   J Mol Biol 359 (5), 1364-1377 (2006)
   PUBMED   16730026
REFERENCE   5  (bases 1 to 1976)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   6  (bases 1 to 1976)
  AUTHORS   Merrill,M.J., Yeh,G.C. and Phang,J.M.
  TITLE     Purified human erythrocyte pyrroline-5-carboxylate reductase.
            Preferential oxidation of NADPH
  JOURNAL   J Biol Chem 264 (16), 9352-9358 (1989)
   PUBMED   2722838
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000010.1.
            
            On or before Oct 27, 2022 this sequence version replaced
            XM_039085691.1, NM_001105857.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643286.32939.1,
                                           SRR26643285.46133.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760433,
                                           SAMN12840105 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-35                JAXUCZ010000010.1  106420778-106420812 c
            36-165              JAXUCZ010000010.1  106420246-106420375 c
            166-236             JAXUCZ010000010.1  106419966-106420036 c
            237-416             JAXUCZ010000010.1  106419174-106419353 c
            417-638             JAXUCZ010000010.1  106418748-106418969 c
            639-731             JAXUCZ010000010.1  106418547-106418639 c
            732-895             JAXUCZ010000010.1  106418227-106418390 c
            896-1976            JAXUCZ010000010.1  106416056-106417136 c
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q32.3"
     gene            1..1976
                     /gene="Pycr1"
                     /note="pyrroline-5-carboxylate reductase 1"
                     /db_xref="GeneID:287877"
                     /db_xref="RGD:1307863"
     exon            1..35
                     /gene="Pycr1"
                     /inference="alignment:Splign:2.1.0"
     exon            36..165
                     /gene="Pycr1"
                     /inference="alignment:Splign:2.1.0"
     CDS             99..1028
                     /gene="Pycr1"
                     /EC_number="1.5.1.2"
                     /codon_start=1
                     /product="pyrroline-5-carboxylate reductase 1,
                     mitochondrial"
                     /protein_id="NP_001099327.1"
                     /db_xref="GeneID:287877"
                     /db_xref="RGD:1307863"
                     /translation="MSVGFIGAGQLAFALAKGFTAAGVLAAHKIMASSPDMDQATVSA
                     LRKIGVNLTPHNKETVCHSDVLFLAVKPHIIPFILDEIGANIEDRHIVVSCAAGVTIN
                     SIEKKLTAFQPAPKVIRCMTNTPVVVREGVTVYATGTHAQVEDGKLVEQLMGSVGFCT
                     EVEEDLIDAVTGLSGSGPAYAFTALDALADGGVKMGLPRRLAVRLGAQALLGAAKMLL
                     DSEQHPGQLKDNVCSPGGATIHALHVLESGGFRSLLINAVEASCIRTRELQTMADQET
                     VSPAAIKKTVLDKVKLDSSAGVSLSSGHVKPMS"
     exon            166..236
                     /gene="Pycr1"
                     /inference="alignment:Splign:2.1.0"
     exon            237..416
                     /gene="Pycr1"
                     /inference="alignment:Splign:2.1.0"
     exon            417..638
                     /gene="Pycr1"
                     /inference="alignment:Splign:2.1.0"
     exon            639..731
                     /gene="Pycr1"
                     /inference="alignment:Splign:2.1.0"
     exon            732..895
                     /gene="Pycr1"
                     /inference="alignment:Splign:2.1.0"
     exon            896..1976
                     /gene="Pycr1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggactgtcgt aggtgtgcgc cagagtagcc acgctttctc tgctgcccta cagagccgga
       61 cgtcagctgt gaaggttgtg ggtcctggaa ctccagacat gagcgtaggc ttcatcgggg
      121 cagggcagct agcctttgcc ctagccaagg gcttcacagc tgcaggtgtt ctggctgctc
      181 acaagataat ggccagctcc ccagacatgg accaagccac agtctctgcc ctccggaaga
      241 taggggtgaa cctgacaccc cacaacaagg agacggtgtg ccacagcgat gtgctcttcc
      301 tggctgtgaa gccccacatc atccccttca tcctggatga aattggtgct aacattgagg
      361 acaggcacat tgtagtgtcc tgtgcggcag gtgtcaccat caactccatt gaaaagaaac
      421 tgacagcgtt ccaacctgct cccaaagtca tccgatgcat gactaacacc ccggtcgtgg
      481 tacgggaggg ggttactgtg tacgccacgg gtactcacgc tcaggtggaa gatggcaagc
      541 ttgtggagca gctcatgggc agcgtgggct tctgcacaga ggtggaagaa gatctgatcg
      601 atgccgtcac ggggctcagt ggcagcggcc ctgcctatgc tttcacagcc ctggatgctc
      661 tggctgatgg tggtgtgaaa atggggcttc caagacgtct ggcagtccgc cttggggccc
      721 aggccctcct gggggcagcc aagatgctac tagattcaga acaacatccg ggccagctca
      781 aggacaatgt ctgctctcct ggtggggcca ccatccatgc cttgcatgtg ctggagagtg
      841 ggggctttcg ttccctgctt atcaatgctg tggaagcctc ctgtatccgc acacgggagc
      901 tgcagaccat ggctgatcag gaaaccgtct cccctgctgc cataaagaag actgtactgg
      961 acaaggtgaa gctagactcc tctgctgggg tctctctgtc ttctggccat gttaagccga
     1021 tgtcctgaaa tctggtccca gctgacaagg aatgacttgt ctgccaacct tcctcctgtt
     1081 ccctacttcc tttcctcccc ccaccctttc cctccccctc cccctccccc tccccttcct
     1141 ttcctttcct tttctttccc tcccccttcc ctcctctccc cctccctctc ccccctccct
     1201 ccccttctcc ttcctcttcc ccttcctttc ccttcctttc ccttccttaa aagcagggtt
     1261 ttgcagtgta gtccaggtta gctctgaact cttcaccccc ccactctagt ctcccaagtg
     1321 ctgggattat ggttgagggt tagcatgcat gccaggcctt ttttcttcta agatagtggc
     1381 ttgatgctgc ccagacacga ctcgaactta caggctcagg gcatactctg atttcaactc
     1441 ctgtaagtgc acaacacctg agtgattctg ttcttctctt tcttctcttc ttgcttgaag
     1501 cttctgtcct ggtgctctca gacaacacag aatatcccca cagcaggccc ttgcccacag
     1561 ccaggtcagc tgggaaggga acatcactta caactaggga cctccattga tactcaagtg
     1621 ccagctggaa acagggtggg tgctcagtcc gccacctctt cagcttcccc tggagctcag
     1681 gcctgtgggg gtctttatcc tccctagttt tggggtcagc tagctggctt agccacggtc
     1741 ccttcctgtg atccactcac agggttcctt tctgtgacag ccagctagtc ccagacaatc
     1801 tgtacctccc tccttctagg gacttaatgc tttaccaata atcccactag gcatttggga
     1861 aaagaacttc tcaggtagcc gacgaaactc ctgcatgttc ctcgactcaa caaatgctgt
     1921 tacttctctt cctgtacttt cagttgaata attaaaaaaa aaaaaaatcc cccaaa
//
