LOCUS       NM_001271261            6563 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus peroxidasin (Pxdn), mRNA.
ACCESSION   NM_001271261 XM_001053103 XM_002726729
VERSION     NM_001271261.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Li,T., Liu,B., Li,N.S., Luo,X.J., Peng,J.W. and Peng,J.
  TITLE     Vascular peroxidase 1 promotes phenotypic transformation of
            pulmonary artery smooth muscle cells via ERK pathway in
            hypoxia-induced pulmonary hypertensive rats
  JOURNAL   Life Sci 307, 120910 (2022)
   PUBMED   36029851
  REMARK    GeneRIF: Vascular peroxidase 1 promotes phenotypic transformation
            of pulmonary artery smooth muscle cells via ERK pathway in
            hypoxia-induced pulmonary hypertensive rats.
REFERENCE   2  (bases 1 to 6563)
  AUTHORS   Wang,E.L., Jia,M.M., Luo,F.M., Li,T., Peng,J.J., Luo,X.J.,
            Song,F.L., Yang,J.F., Peng,J. and Liu,B.
  TITLE     Coordination between NADPH oxidase and vascular peroxidase 1
            promotes dysfunctions of endothelial progenitor cells in
            hypoxia-induced pulmonary hypertensive rats
  JOURNAL   Eur J Pharmacol 857, 172459 (2019)
   PUBMED   31216444
  REMARK    GeneRIF: NOX/VPO1 pathway-derived reactive oxygen species promote
            the oxidative injury and dysfunctions of EPCs in PH, which may
            contribute to endothelial dysfunctions in PH
REFERENCE   3  (bases 1 to 6563)
  AUTHORS   You,B., Liu,Y., Chen,J., Huang,X., Peng,H., Liu,Z., Tang,Y.,
            Zhang,K., Xu,Q., Li,X., Cheng,G., Shi,R. and Zhang,G.
  TITLE     Vascular peroxidase 1 mediates hypoxia-induced pulmonary artery
            smooth muscle cell proliferation, apoptosis resistance and
            migration
  JOURNAL   Cardiovasc Res 114 (1), 188-199 (2018)
   PUBMED   29186367
  REMARK    GeneRIF: VPO1 promotes hypoxia-induced proliferation, apoptosis
            resistance, and migration in pulmonary artery smooth muscle cells
            via the NOX4/VPO1/HOCl/NF-kappaB signalling pathway.
REFERENCE   4  (bases 1 to 6563)
  AUTHORS   Yang,W., Liu,Z., Xu,Q., Peng,H., Chen,L., Huang,X., Yang,T., Yu,Z.,
            Cheng,G., Zhang,G. and Shi,R.
  TITLE     Involvement of vascular peroxidase 1 in angiotensin II-induced
            hypertrophy of H9c2 cells
  JOURNAL   J Am Soc Hypertens 11 (8), 519-529 (2017)
   PUBMED   27651140
  REMARK    GeneRIF: VPO1 promotes cardiac remodeling by accelerating the Ang
            II-induced hypertrophy of H9c2 cell via the NOX2/VPO1/HOCl/ERK1/2
            signaling pathways.
REFERENCE   5  (bases 1 to 6563)
  AUTHORS   Zanivan,S., Maione,F., Hein,M.Y., Hernandez-Fernaud,J.R.,
            Ostasiewicz,P., Giraudo,E. and Mann,M.
  TITLE     SILAC-based proteomics of human primary endothelial cell
            morphogenesis unveils tumor angiogenic markers
  JOURNAL   Mol Cell Proteomics 12 (12), 3599-3611 (2013)
   PUBMED   23979707
REFERENCE   6  (bases 1 to 6563)
  AUTHORS   Yang,L., Bai,Y., Li,N., Hu,C., Peng,J., Cheng,G., Zhang,G. and
            Shi,R.
  TITLE     Vascular VPO1 expression is related to the endothelial dysfunction
            in spontaneously hypertensive rats
  JOURNAL   Biochem Biophys Res Commun 439 (4), 511-516 (2013)
   PUBMED   24021280
  REMARK    GeneRIF: The over-expression of VPO1 during the development of
            hypertension, accompanied by the endothelial dysfunction, the
            decreased NO levels, the elevated NOX and ROS activities, indicates
            a clear connection between VPO1 gene and hypertension.(VPO1)
REFERENCE   7  (bases 1 to 6563)
  AUTHORS   Bhave,G., Cummings,C.F., Vanacore,R.M., Kumagai-Cresse,C.,
            Ero-Tolliver,I.A., Rafi,M., Kang,J.S., Pedchenko,V., Fessler,L.I.,
            Fessler,J.H. and Hudson,B.G.
  TITLE     Peroxidasin forms sulfilimine chemical bonds using hypohalous acids
            in tissue genesis
  JOURNAL   Nat Chem Biol 8 (9), 784-790 (2012)
   PUBMED   22842973
REFERENCE   8  (bases 1 to 6563)
  AUTHORS   Shi,R., Hu,C., Yuan,Q., Yang,T., Peng,J., Li,Y., Bai,Y., Cao,Z.,
            Cheng,G. and Zhang,G.
  TITLE     Involvement of vascular peroxidase 1 in angiotensin II-induced
            vascular smooth muscle cell proliferation
  JOURNAL   Cardiovasc Res 91 (1), 27-36 (2011)
   PUBMED   21292788
  REMARK    GeneRIF: Vascular peroxidase 1/peroxidasin is a novel regulator of
            angiotensin II-induced vascular smooth muscle cell proliferation
            via NADPH oxidase-hydrogen peroxide pathways.
REFERENCE   9  (bases 1 to 6563)
  AUTHORS   Peterfi,Z., Donko,A., Orient,A., Sum,A., Prokai,A., Molnar,B.,
            Vereb,Z., Rajnavolgyi,E., Kovacs,K.J., Muller,V., Szabo,A.J. and
            Geiszt,M.
  TITLE     Peroxidasin is secreted and incorporated into the extracellular
            matrix of myofibroblasts and fibrotic kidney
  JOURNAL   Am J Pathol 175 (2), 725-735 (2009)
   PUBMED   19590037
REFERENCE   10 (bases 1 to 6563)
  AUTHORS   Cheng,G., Salerno,J.C., Cao,Z., Pagano,P.J. and Lambeth,J.D.
  TITLE     Identification and characterization of VPO1, a new animal
            heme-containing peroxidase
  JOURNAL   Free Radic Biol Med 45 (12), 1682-1694 (2008)
   PUBMED   18929642
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000006.1 and FQ140997.1.
            
            On or before Sep 12, 2012 this sequence version replaced
            XM_001053103.3, XM_002726729.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript from the same
            strain was available for the full length of the gene. The extent of
            this transcript is supported by transcript alignments and
            orthologous data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643292.25719.1,
                                           SRR26643285.24346.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-301               JAXUCZ010000006.1  52308374-52308674
            302-373             JAXUCZ010000006.1  52341263-52341334
            374-445             JAXUCZ010000006.1  52342658-52342729
            446-517             JAXUCZ010000006.1  52346534-52346605
            518-589             JAXUCZ010000006.1  52349559-52349630
            590-661             JAXUCZ010000006.1  52349974-52350045
            662-831             JAXUCZ010000006.1  52351653-52351822
            832-949             JAXUCZ010000006.1  52352073-52352190
            950-1119            JAXUCZ010000006.1  52354347-52354516
            1120-1392           JAXUCZ010000006.1  52358756-52359028
            1393-1509           JAXUCZ010000006.1  52361910-52362026
            1510-1668           JAXUCZ010000006.1  52362892-52363050
            1669-1781           JAXUCZ010000006.1  52363746-52363858
            1782-1938           JAXUCZ010000006.1  52364746-52364902
            1939-2047           JAXUCZ010000006.1  52367302-52367410
            2048-2205           JAXUCZ010000006.1  52368008-52368165
            2206-3500           JAXUCZ010000006.1  52370104-52371398
            3501-4316           FQ140997.1         1-816
            4317-4418           JAXUCZ010000006.1  52382937-52383038
            4419-6563           JAXUCZ010000006.1  52383799-52385943
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q16"
     gene            1..6563
                     /gene="Pxdn"
                     /gene_synonym="Perc64; Vpo1"
                     /note="peroxidasin"
                     /db_xref="GeneID:554172"
                     /db_xref="RGD:1560694"
     exon            1..301
                     /gene="Pxdn"
                     /gene_synonym="Perc64; Vpo1"
                     /inference="alignment:Splign:2.1.0"
     CDS             111..4538
                     /gene="Pxdn"
                     /gene_synonym="Perc64; Vpo1"
                     /EC_number="1.11.1.7"
                     /note="PE responsive protein c64; melanoma-associated
                     antigen MG50; peroxidasin homolog"
                     /codon_start=1
                     /product="peroxidasin homolog precursor"
                     /protein_id="NP_001258190.1"
                     /db_xref="GeneID:554172"
                     /db_xref="RGD:1560694"
                     /translation="MAVRPTRRCLLALVLCFAWWAMAVVASKQGAGCPSRCLCFRTTV
                     RCMHLLLEAVPAVAPQTSILDLRFNRIREIQPGAFRRLRNLNTLLLNNNQIKKIPSGA
                     FEDLENLKYLYLYKNEIQSIDRQAFKGLASLEQLYLHFNQIETLDPESFQHLPKLERL
                     FLHNNRITHLVPGTFSQLESMKRLRLDSNALHCDCEILWLADLLKTYAQSGNAQAAAT
                     CEYPRRIQGRSVATITPEELNCERPRITSEPQDADVTSGNTVYFTCRAEGNPKPEIIW
                     LRNNNELSMKTDSRLNLLDDGTLMIQNTQEADEGVYQCMAKNVAGEAKTHEVTLRYLG
                     SPARPTFVIQPQNTEVLVGESVTLECSATGHPLPQITWTRGDRTPLPIDPRVNITPSG
                     GLYIQNVAQSDSGEYTCFASNSVDSIHATAFIIVQALPQFTVTPQSRVVIEGQTVDFQ
                     CEAKGYPQPVIAWTKGGSQLSVDRRHLVLSSGTLRISGVALHDQGQYECQAVNIIGSQ
                     KVVAHLTVQPRVTPVFASIPSDMTVEVGTNVQLPCSSQGEPEPAITWNKDGVQVTESG
                     KFHISPEGFLTINDVGTADAGRYECVARNTIGYASVSMVLSVNVPDVSRNGDPYVATS
                     IVEAIATVDRAINSTRTHLFDSRPRSPNDLLALFRYPRDPYTVGQARAGEIFERTLQL
                     IQEHVQHGLMVDLNGTSYHYNDLVSPQYLSLIANLSGCTAHRRVNNCSDMCFHQKYRT
                     HDGTCNNLQHPMWGASLTAFERLLKAVYENGFNTPRGINSQRQYNGHVLPMPRLVSTT
                     LIGTEVITPDEQFTHMLMQWGQFLDHDLDSTVVALSQARFSDGQHCSSVCSNDPPCFS
                     VMIPPNDPRVRSGARCMFFVRSSPVCGSGMTSLLMNSVYPREQINQLTSYIDASNVYG
                     STDHEARSIRDLASHRGLLRQGIVQRSGKPLLPFATGPPTECMRDENESPIPCFLAGD
                     HRANEQLGLTSMHTLWFREHNRIAAELLKLNPHWDGDTVYHETRKIVGAEIQHITYRH
                     WLPKILGEVGMKMLGEYRGYDPSVNAGIFNAFATAAFRFGHTLINPLLYRLDENFEPI
                     PQGHVPLHKAFFSPFRIVNEGGIDPLLRGLFGVAGKMRIPSQLLNTELTERLFSMAHT
                     VALDLAAINIQRGRDHGIPPYHDYRVYCNLSAAYTFEDLKNEIKSPVIREKLQRLYGS
                     TLNIDLFPALMVEDLVPGSRLGPTLMCLLSTQFRRLRDGDRLWYENPGVFSPAQLTQL
                     KQTSLARILCDNSDNITRVQHDVFRVAEFPHGYSSCEDIPRMDLRVWQDCCEDCRTRG
                     QFNAFSYHFRGRRSLEFSYEEDKPTKRARWRKALSVKHGKHHSNATSVTHEHLEGPAT
                     NDLKEFVLEMQKIITDLRKQINSLESRLSTTECVDDSGESHGSNTKWEKDTCTVCECK
                     NGQVTCFVEACQPAACPQPVKVEGACCPVCLRNTEEEKP"
     exon            302..373
                     /gene="Pxdn"
                     /gene_synonym="Perc64; Vpo1"
                     /inference="alignment:Splign:2.1.0"
     exon            374..445
                     /gene="Pxdn"
                     /gene_synonym="Perc64; Vpo1"
                     /inference="alignment:Splign:2.1.0"
     exon            446..517
                     /gene="Pxdn"
                     /gene_synonym="Perc64; Vpo1"
                     /inference="alignment:Splign:2.1.0"
     exon            518..589
                     /gene="Pxdn"
                     /gene_synonym="Perc64; Vpo1"
                     /inference="alignment:Splign:2.1.0"
     exon            590..661
                     /gene="Pxdn"
                     /gene_synonym="Perc64; Vpo1"
                     /inference="alignment:Splign:2.1.0"
     exon            662..831
                     /gene="Pxdn"
                     /gene_synonym="Perc64; Vpo1"
                     /inference="alignment:Splign:2.1.0"
     exon            832..949
                     /gene="Pxdn"
                     /gene_synonym="Perc64; Vpo1"
                     /inference="alignment:Splign:2.1.0"
     exon            950..1119
                     /gene="Pxdn"
                     /gene_synonym="Perc64; Vpo1"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1392
                     /gene="Pxdn"
                     /gene_synonym="Perc64; Vpo1"
                     /inference="alignment:Splign:2.1.0"
     exon            1393..1509
                     /gene="Pxdn"
                     /gene_synonym="Perc64; Vpo1"
                     /inference="alignment:Splign:2.1.0"
     exon            1510..1668
                     /gene="Pxdn"
                     /gene_synonym="Perc64; Vpo1"
                     /inference="alignment:Splign:2.1.0"
     exon            1669..1781
                     /gene="Pxdn"
                     /gene_synonym="Perc64; Vpo1"
                     /inference="alignment:Splign:2.1.0"
     exon            1782..1938
                     /gene="Pxdn"
                     /gene_synonym="Perc64; Vpo1"
                     /inference="alignment:Splign:2.1.0"
     exon            1939..2047
                     /gene="Pxdn"
                     /gene_synonym="Perc64; Vpo1"
                     /inference="alignment:Splign:2.1.0"
     exon            2048..2205
                     /gene="Pxdn"
                     /gene_synonym="Perc64; Vpo1"
                     /inference="alignment:Splign:2.1.0"
     exon            2206..3709
                     /gene="Pxdn"
                     /gene_synonym="Perc64; Vpo1"
                     /inference="alignment:Splign:2.1.0"
     exon            3710..3844
                     /gene="Pxdn"
                     /gene_synonym="Perc64; Vpo1"
                     /inference="alignment:Splign:2.1.0"
     exon            3845..4053
                     /gene="Pxdn"
                     /gene_synonym="Perc64; Vpo1"
                     /inference="alignment:Splign:2.1.0"
     exon            4054..4174
                     /gene="Pxdn"
                     /gene_synonym="Perc64; Vpo1"
                     /inference="alignment:Splign:2.1.0"
     exon            4175..4304
                     /gene="Pxdn"
                     /gene_synonym="Perc64; Vpo1"
                     /inference="alignment:Splign:2.1.0"
     exon            4305..4418
                     /gene="Pxdn"
                     /gene_synonym="Perc64; Vpo1"
                     /inference="alignment:Splign:2.1.0"
     exon            4419..6563
                     /gene="Pxdn"
                     /gene_synonym="Perc64; Vpo1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gacgtgggcc ggagacctga gggctcagtt gggagccggc ggtggacgcg cccgccgagg
       61 cctcctccgc tgctgtccac gcgtgccagc gcctgtccgc gccgtctgcc atggccgtgc
      121 gccccacgcg ccgctgcctg ctggcactcg tgctgtgctt cgcctggtgg gccatggcgg
      181 tggtcgcctc gaagcaaggg gcaggctgtc caagccgctg cctgtgcttt cgtaccaccg
      241 tgcgctgcat gcatctgttg ttggaggccg tgcccgcggt ggcgccgcag acctccatcc
      301 tagatcttcg gttcaacaga atcagagaga tccaacccgg ggcattcagg aggctgagga
      361 acttgaacac actgcttctt aacaacaacc agatcaagaa gattcccagt ggtgcctttg
      421 aggacctgga gaacttaaaa tacctctatt tgtacaagaa tgagatccag tcaattgaca
      481 ggcaagcatt taagggactt gcctctctag agcaactgta cctgcacttt aatcagatag
      541 agacgctgga cccagaatcc ttccagcacc tgccgaagct ggagagactg tttttacata
      601 ataatcgcat cacacactta gttcctggga cgttcagtca gttggagtcc atgaaacggc
      661 tgcgattaga ctcaaatgca cttcactgtg actgtgaaat attatggcta gcggatctgc
      721 tgaagaccta tgcccaatct ggaaatgcac aagcagcagc cacctgcgag tatcccagac
      781 gcatccaagg acgctctgtg gctaccatca ccccagaaga gctgaactgt gaaagacccc
      841 ggatcacctc agagccacag gatgcagatg tcacctcagg gaacacagtg tacttcacct
      901 gcagagctga gggcaacccc aaacctgaga tcatctggct tcgaaacaat aacgagctga
      961 gcatgaagac agattctcgc ttaaacttgc tggatgacgg cacactgatg atccagaaca
     1021 cacaggaggc cgacgagggt gtctaccagt gcatggcaaa aaacgtggct ggagaggcga
     1081 aaacgcatga ggtgaccctc aggtacttgg gatccccagc ccgacccact tttgtaatcc
     1141 agccgcagaa cacagaggta ctggtgggtg agagtgtcac tctggagtgc agtgccacag
     1201 gtcaccctct gcctcagatc acgtggactc gaggtgaccg gacacccttg ccgattgacc
     1261 ctcgagtgaa tatcactccc tctggaggac tgtatataca gaacgtcgcc cagagcgata
     1321 gtggcgagta cacgtgcttt gcatccaaca gcgtggacag catccatgcc acggccttca
     1381 tcattgtaca ggcccttcct cagttcactg taacccctca gagccgagtg gtcattgaag
     1441 gacagactgt ggacttccag tgtgaggcta agggctaccc tcagccagtc atcgcctgga
     1501 ccaagggagg gagccagctc tcagtggaca ggcggcacct ggtgctgtcc tcaggaacac
     1561 tcaggatttc tggggtggcc ctgcatgacc agggccagta tgagtgccag gccgtcaaca
     1621 tcattggctc ccagaaggtg gtggcccacc tgactgtaca gcccagagtc accccagtat
     1681 ttgccagcat tcccagtgac atgactgtag aggtgggcac caacgtgcag ctgccttgta
     1741 gctcccaggg ggaaccagag ccagccatca cctggaacaa ggatggtgtt caggtaacag
     1801 aaagtggaaa atttcacatc agcccggaag gattcttgac catcaatgat gttggcactg
     1861 ctgatgcagg tcgctatgag tgtgtagctc ggaacacaat tggatatgcc tctgtgagca
     1921 tggtactcag cgtgaacgtt cctgatgtaa gtcgaaatgg ggatccatac gttgctacct
     1981 ctattgttga agccattgca actgttgaca gagccatcaa ctctacaagg acacacttgt
     2041 ttgacagccg acctcgttct ccaaatgacc tacttgctct gttccggtac ccacgggatc
     2101 cgtacaccgt gggacaagcc agggcaggag agatattcga gcggaccctg cagctgatcc
     2161 aggaacatgt tcagcatggc ttgatggtgg acttgaatgg aacaagttac cactacaatg
     2221 atctggtgtc cccgcagtac ctgagcctca tcgccaacct gtcaggctgc actgcacacc
     2281 gtcgtgtgaa caactgctca gacatgtgct tccaccagaa gtataggacg cacgatggca
     2341 cgtgcaacaa tctgcagcac ccgatgtggg gagcctcact gaccgccttc gagcgcctgc
     2401 tgaaggctgt gtatgagaat ggattcaaca cgccccgtgg cattaattcc cagcgtcagt
     2461 acaatgggca tgtactaccc atgccccgcc tggtgtccac cacactgatc gggacagagg
     2521 tgatcacccc agatgagcag tttacacaca tgctgatgca atggggccag ttccttgacc
     2581 atgacctaga ctctacagtg gtggccctga gccaggcccg cttctctgat gggcagcatt
     2641 gcagctcagt gtgcagcaat gaccctccct gtttctcggt catgatcccc cccaacgatc
     2701 ccagagtgcg gagtggggcc cgatgcatgt tcttcgtgcg gtcaagccct gtgtgcggca
     2761 gtggcatgac atccctgctc atgaactctg tgtaccctcg agagcagatc aaccagctca
     2821 cttcctacat tgacgcatcc aatgtgtatg gcagcacaga ccacgaagcc cggagcatcc
     2881 gggacctggc cagtcatcgt ggcctgctac gtcagggtat cgtgcagagg tctggcaagc
     2941 ccctgcttcc ctttgccacg gggccaccca ctgagtgtat gcgtgatgag aatgagagtc
     3001 caataccgtg ctttctggcc ggtgaccatc gtgccaacga gcagctgggc ctgaccagca
     3061 tgcatacgct gtggttccgg gagcacaacc gcatcgcagc agagctgttg aagctgaacc
     3121 cgcactggga tggggacact gtctaccatg agacccgcaa gatagtgggg gcagagatcc
     3181 agcacattac ctaccggcac tggctaccca agatcctggg ggaggtgggc atgaagatgc
     3241 taggtgagta ccggggctac gaccccagcg tcaatgctgg catctttaat gcctttgcca
     3301 ccgcagcctt caggttcggt cacactctga tcaaccctct gctctaccgg ctggatgaga
     3361 actttgagcc catcccacag ggccacgtgc ccctccacaa agccttcttc tcacccttcc
     3421 ggattgtcaa tgaggggggc atcgacccac ttctccgagg gctgtttgga gtggcaggga
     3481 agatgcgcat tccctctcaa ctgctgaaca ctgagctcac agagaggctt ttctccatgg
     3541 cacacacagt ggcccttgac ctggctgcca tcaatatcca gcgaggccgg gaccatggca
     3601 tcccacccta ccatgactac agggtctact gcaacctgtc agccgcttat acctttgagg
     3661 acctgaaaaa tgagatcaag agtcccgtga tccgggagaa actgcagagg ctatatggct
     3721 cgacactcaa catcgatctg ttcccggccc tcatggtgga agacctggtc cccggcagcc
     3781 gcttggggcc cacactcatg tgcctgctca gcacacagtt ccgacgcctg cgggatggag
     3841 acaggttgtg gtatgagaac ccaggcgtgt tctcccctgc ccagctgact cagctcaagc
     3901 agacatccct ggcgaggatc ctttgtgaca actcagacaa catcacccgc gtgcagcacg
     3961 atgtgttcag ggtggcagag ttcccacacg gttacagcag ctgcgaggac atccccagaa
     4021 tggacctgcg agtgtggcag gactgttgtg aagactgtag gaccagggga caattcaatg
     4081 ccttctccta ccatttccgg ggaagaaggt ctctagaatt cagctatgag gaggataagc
     4141 ccacaaagag agccaggtgg cggaaagcac taagtgtaaa acatgggaaa catcatagca
     4201 atgccacatc agtcacccat gagcacttgg aagggccagc aactaatgat ctcaaggagt
     4261 ttgttctaga aatgcaaaag atcatcacag acctcagaaa acagataaac agcctggagt
     4321 ctcggctcag caccacagag tgtgtggatg acagtggtga atcccacggc agcaacacga
     4381 agtgggaaaa agacacatgc acagtttgcg agtgcaaaaa tggccaggtc acctgctttg
     4441 tggaagcttg ccagcctgca gcctgcccgc agcctgtgaa agtcgaaggc gcttgctgtc
     4501 ctgtctgctt aaggaacact gaagaggaaa agccttagtg ttcccgagct gagctcctca
     4561 aagctggtcc acagaatcct tgtgagccta gatgacaacc tgcggaactt cagaccacag
     4621 gacaagttgg gatcccagac atttcaggac ctccgctgtg ccatcgcaga atcacccggg
     4681 gctgcttcag accccgtgtt cgtagaagga aatcgagcag gagggagtgg gtgcaggccc
     4741 tggccctcac ttcatgttag acttctcagg tttatactta agtgttttta aatggaaaat
     4801 tggtgctact attaaatcac acagttaaga catgggtttc gaaattgctt ctgcctgatg
     4861 atgccacctc tgtttaaatg aggcgggagg tctttgactg gcttcattta aacagaagca
     4921 tttggcaaat ggcacaggat cttataaaat gacatcctag gtcacaatcc ctacagggat
     4981 cctcgggctt tctcactact ccttcctgtc tgctatcttc accttcaggg cccttcagca
     5041 tctaacaata ccaacacaat cctgcacctc aggaccatgt ctgcccttct acacacgact
     5101 gtcacattat taagccgcat ttagaaataa tcttgtttct acttagaacg tgtgtaaata
     5161 ctgccttgct gtaaactgaa tgtgaaatcc tttggtccca tgccatgttg agtgacctga
     5221 acagtccctt gcattgttgg taggatggtc tgcagccatc ttctgccttg gttcctggcc
     5281 atagtgtagg gagggtgatt cactctctgg gtcttttgag taggtgacag ataggcagag
     5341 cctgaatgtc aggaaatcac attgtgtgaa tgctttaaag gaaaaaaaaa gaatccctat
     5401 acctcatatt ttctattgca tgatgtttta taaaaatttg cccattttag atgttagaaa
     5461 aattacttcc actatgcaaa ttttttaatt cagtgaaaag caactattat acctcatagt
     5521 ctcttgtttt taattgaccg aaatattcca ttcacaaggt tctgaggact ctcctgaaca
     5581 agcaagtctc accctataca cactgatgtg cccagcactg tatgtcagcc attgtgggta
     5641 catcaagagg gtcacctgcc caaggactgg ggaggaagac ctcaaggaca cagatgagtc
     5701 aatgtcagtt ggaaaaagac aaaaatggat gcccttatag ggagaccaca gtcagatagg
     5761 gagatgttca gttccttgca gcctctggct tacgttgggc agccaggcca ttgtggtgtg
     5821 tgcgtgtgta tgatccctgt gctgatatgg ttaccttcct agagatagac acaaagccac
     5881 atgtgtgagg ctgctgagca aagacagcat cctagcatgg gcttgttcac gagggattca
     5941 ccacactgca tatgtaaaag tggtcacctt gtcttcccct catgatattt ggaaactggt
     6001 ggtccagcag gggtgggcat ttgtgaaata ttaagttgaa cttagtgata agatttcaga
     6061 acagacttct gtgaagtagg agatgtaacc atgcatttga aatctgatgg ccatgtaatc
     6121 actcaggcat agaatggtgg gagagcctgc cctggatatc tggtatttca caagagcacc
     6181 aggggtatgt cttgtgctaa ggcacgcagt gtgtacatgg agcttagaca ggtgccaagg
     6241 gtttttgtcc tctgctcctg tgtgagaagc tggctgtgat ttacattaac ttctgtattt
     6301 caaaccaaga tgtctgcaga tctccatttt gatgttacag cctcattgca agctgtgggc
     6361 agtacccaga caccctttct gactagccac tgcactgggc ttctgtgatt caaagtagtg
     6421 tacatattta tttacttttc tgactgttgc caacagccaa atgccatttt atgtttcttg
     6481 tatccagccc attaccaaag aggtgtttgc actttgtcat gacgcctttc agttcaaata
     6541 aaaggaccac gtcgtcaagt gga
//
