LOCUS       NM_001401164            3191 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus pseudouridine synthase 10 (Pus10), transcript
            variant 1, mRNA.
ACCESSION   NM_001401164 XM_008770429
VERSION     NM_001401164.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Song,J., Zhuang,Y., Zhu,C., Meng,H., Lu,B., Xie,B., Peng,J., Li,M.
            and Yi,C.
  TITLE     Differential roles of human PUS10 in miRNA processing and tRNA
            pseudouridylation
  JOURNAL   Nat Chem Biol 16 (2), 160-169 (2020)
   PUBMED   31819270
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Deogharia,M., Mukhopadhyay,S., Joardar,A. and Gupta,R.
  TITLE     The human ortholog of archaeal Pus10 produces pseudouridine 54 in
            select tRNAs where its recognition sequence contains a modified
            residue
  JOURNAL   RNA 25 (3), 336-351 (2019)
   PUBMED   30530625
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000014.1.
            
            On Feb 4, 2022 this sequence version replaced XM_008770429.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643296.33701.1,
                                           SRR26643292.53044.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-398               JAXUCZ010000014.1  101822525-101822922
            399-539             JAXUCZ010000014.1  101825440-101825580
            540-791             JAXUCZ010000014.1  101829865-101830116
            792-878             JAXUCZ010000014.1  101830588-101830674
            879-913             JAXUCZ010000014.1  101850816-101850850
            914-1025            JAXUCZ010000014.1  101853025-101853136
            1026-1087           JAXUCZ010000014.1  101855813-101855874
            1088-1133           JAXUCZ010000014.1  101856406-101856451
            1134-1198           JAXUCZ010000014.1  101857965-101858029
            1199-1284           JAXUCZ010000014.1  101860092-101860177
            1285-1410           JAXUCZ010000014.1  101860998-101861123
            1411-1467           JAXUCZ010000014.1  101866234-101866290
            1468-1544           JAXUCZ010000014.1  101867293-101867369
            1545-1600           JAXUCZ010000014.1  101867459-101867514
            1601-1718           JAXUCZ010000014.1  101873341-101873458
            1719-1861           JAXUCZ010000014.1  101878643-101878785
            1862-1961           JAXUCZ010000014.1  101881912-101882011
            1962-3191           JAXUCZ010000014.1  101883958-101885187
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="14"
                     /map="14q22"
     gene            1..3191
                     /gene="Pus10"
                     /gene_synonym="RGD1306402"
                     /note="pseudouridine synthase 10"
                     /db_xref="GeneID:305583"
                     /db_xref="RGD:1306402"
     exon            1..398
                     /gene="Pus10"
                     /gene_synonym="RGD1306402"
                     /inference="alignment:Splign:2.1.0"
     exon            399..539
                     /gene="Pus10"
                     /gene_synonym="RGD1306402"
                     /inference="alignment:Splign:2.1.0"
     CDS             414..2000
                     /gene="Pus10"
                     /gene_synonym="RGD1306402"
                     /EC_number="5.4.99.25"
                     /note="isoform 1 is encoded by transcript variant 1;
                     putative tRNA pseudouridine synthase Pus10; putative tRNA
                     pseudouridine synthase Pus10-like; pseudouridylate
                     synthase 10"
                     /codon_start=1
                     /product="tRNA pseudouridine synthase Pus10 isoform 1"
                     /protein_id="NP_001388093.1"
                     /db_xref="GeneID:305583"
                     /db_xref="RGD:1306402"
                     /translation="MFPLKEENRRVAQLLLRSGTCPRCIFRFCGVDLPAPYKRPYKEL
                     LDDLQTFLESENRELILEGPDPPLKKIRLHEDGVDTLSANGEEGVSVTEDENMAATKP
                     SNWRVCNVCLGILQGFCEKDFITEVCQKVEASGFEFTSVVLSVSFPPQLSVREHAAWL
                     LVKQEMGKQSLSLGRNDVVQLKEAYKWITHPLFSEELGVPTDGKSLFEVSVVFAHPET
                     LQDCHFLGEVCPDCFKPAKNKQSVFTRMAVLKALSRIKEEDFLTQFPCPPDSPKTICT
                     VLEVECTHGAVFVAGRYNKYSRNLPQTPWIIDGERKMESSVEELISDHLLAVFKAESF
                     NFSSSGREDVDVRTLGNGRPFAVELLNPHRVHFTSQEIKELQQKINKSSDKIQVRDLQ
                     LVTREAIGHMKEGEEEKTKTYSALIWTNRAIQKKDIGFLDDLKDLKIDQKTPLRVLHR
                     RPLAVRTRAIHSMKTHYLDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTA
                     DIVELDVESVDVDWPPALDD"
     exon            540..791
                     /gene="Pus10"
                     /gene_synonym="RGD1306402"
                     /inference="alignment:Splign:2.1.0"
     exon            792..878
                     /gene="Pus10"
                     /gene_synonym="RGD1306402"
                     /inference="alignment:Splign:2.1.0"
     exon            879..913
                     /gene="Pus10"
                     /gene_synonym="RGD1306402"
                     /inference="alignment:Splign:2.1.0"
     exon            914..1025
                     /gene="Pus10"
                     /gene_synonym="RGD1306402"
                     /inference="alignment:Splign:2.1.0"
     exon            1026..1087
                     /gene="Pus10"
                     /gene_synonym="RGD1306402"
                     /inference="alignment:Splign:2.1.0"
     exon            1088..1133
                     /gene="Pus10"
                     /gene_synonym="RGD1306402"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..1198
                     /gene="Pus10"
                     /gene_synonym="RGD1306402"
                     /inference="alignment:Splign:2.1.0"
     exon            1199..1284
                     /gene="Pus10"
                     /gene_synonym="RGD1306402"
                     /inference="alignment:Splign:2.1.0"
     exon            1285..1410
                     /gene="Pus10"
                     /gene_synonym="RGD1306402"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1467
                     /gene="Pus10"
                     /gene_synonym="RGD1306402"
                     /inference="alignment:Splign:2.1.0"
     exon            1468..1544
                     /gene="Pus10"
                     /gene_synonym="RGD1306402"
                     /inference="alignment:Splign:2.1.0"
     exon            1545..1600
                     /gene="Pus10"
                     /gene_synonym="RGD1306402"
                     /inference="alignment:Splign:2.1.0"
     exon            1601..1718
                     /gene="Pus10"
                     /gene_synonym="RGD1306402"
                     /inference="alignment:Splign:2.1.0"
     exon            1719..1861
                     /gene="Pus10"
                     /gene_synonym="RGD1306402"
                     /inference="alignment:Splign:2.1.0"
     exon            1862..1961
                     /gene="Pus10"
                     /gene_synonym="RGD1306402"
                     /inference="alignment:Splign:2.1.0"
     exon            1962..3191
                     /gene="Pus10"
                     /gene_synonym="RGD1306402"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 caggatgttc ctctttcttt tccctagttt gggtttcaaa tttgagtagg ggctcatcga
       61 cgtttctggc agcagctact acgacctagg tgcctagtct atcggaggct cctttgcggg
      121 tcgacccttt aaggctagca gtaaagcccc tcgcagtcca gcgttcgcgg agctacctgg
      181 agtaccaggc tgccagccgg acaggcttac agatcaccct gtatcgaaag ggggttcatg
      241 cattcctctc gtgagggggc ctggtacttt cgttgtaaag cccttgtttc cttggggaac
      301 agaacggctg gacgcaggag gtgcgctgtc tttagtcttc cccgttccca cccaggactg
      361 aaaagggtta agtcttggct gcagcatctg gagagaagtt ggagctgttc agtatgttcc
      421 cactgaagga ggagaacaga cgtgtggccc agctgctgct caggtctggc acctgcccaa
      481 gatgcatctt cagattctgc ggcgtggatc ttccggcccc ttacaaacgc ccatacaagg
      541 agttgctcga tgatctacaa acatttctgg aatctgagaa ccgtgagtta attctggaag
      601 gtccagaccc accgttgaag aagattcgtc tacacgaaga tggggttgat actttgagtg
      661 caaatggaga ggagggcgtc tctgttactg aagatgaaaa catggcggcc acgaagcctt
      721 caaattggag agtgtgcaat gtttgcttag ggattcttca gggattctgc gagaaggact
      781 ttatcacaga ggtgtgccaa aaggttgaag cctctgggtt tgaattcacc agcgtggttt
      841 tatctgtgtc cttcccaccg cagctgtctg tgagagagca tgctgcatgg ttgctggtaa
      901 aacaggaaat gggaaagcag agcctgtccc tgggaagaaa tgatgtcgtt cagctgaagg
      961 aagcctacaa gtggataact caccccctgt tttcagagga gctgggagtc cccactgatg
     1021 ggaagagctt gttcgaagtg agtgtggtct ttgctcaccc agaaacactt caagactgcc
     1081 atttcctagg tgaagtttgc ccagactgtt tcaaaccagc caaaaacaaa cagtcagtat
     1141 tcaccagaat ggccgttttg aaagcgttga gtaggataaa agaagaagac ttcctcacgc
     1201 aatttccttg tcctccagac tcccccaaaa ctatatgcac tgtccttgaa gttgaatgca
     1261 cgcatggtgc tgttttcgtt gctggcagat acaataaata ctccaggaat ctgccacaaa
     1321 ctccttggat aattgatggg gaaaggaaaa tggaatcttc ggtggaagaa ttaatttcag
     1381 atcatctgct ggcagtattc aaagcagaga gttttaattt ctcatcctct ggacgagaag
     1441 atgtagatgt gagaacttta ggaaatggaa ggccctttgc ggttgagctg ctgaatcctc
     1501 atcgagtgca cttcacttca caagaaatta aggagctgca gcagaaaatt aataaatcat
     1561 ctgacaaaat ccaagtccgt gacttgcagc ttgtcaccag agaggcaatt gggcatatga
     1621 aggaaggcga agaagaaaag actaagacct acagtgcctt gatatggacg aatagagcca
     1681 tacagaagaa ggacattggg ttcctggatg acttaaagga cttgaagatt gaccagaaaa
     1741 cacctctgcg ggtccttcac cgcaggcctc tggctgtgag aacacgggcc attcactcta
     1801 tgaagacaca ctacctggat gagcaccatt ttcgtctgca tctgaagacc caggcaggga
     1861 cttacattaa agaatttgta cacggagact tcgggagaac caagccaaac attggctctc
     1921 tgatgaacgt gactgcagac attgttgagc tagatgtgga gtctgtagat gttgactggc
     1981 cgccagctct ggatgactag ctgtcgcatt tgactacaga ggggttcctg gcatgatgtg
     2041 gaatagtctg gaaaatggct gtttacccac catagcggtg tcttcaaaac cacttggatc
     2101 atgttgatct gttcctaaag ttcactgtaa catctcagga tctacgtgta cgcatatttt
     2161 gtgttgtgtt gttctaagaa tgtcttatgt tttctcattc cctgtctcat gaccacaaaa
     2221 ccaaaataag aggaatcaaa ccattttcct tctaaatcgt gttccttcga gaggcatgca
     2281 gtgggaggca tgcagtggga ggcatgcagt gggaggcatg cagtgggagg cactgtcaat
     2341 caggagagct tcgatctcct ccagcacagt ctcctccagg aggcggttcc tttaaagcaa
     2401 tttgctaagg ctatgagatc atctgtgtaa atgttacaac tagactcatg tggaagagtt
     2461 tcctaggata ttccaacatg tcaaatgtaa atctgtatta atatttccca attaccctcc
     2521 agcaggcttc tattattaat agaaatactt taaggaatga tttgtcatag tccttatgac
     2581 ctcatgttac tattttgaca atcgaaaatt gtcagtttgt gtctcctgta aaggcaagtg
     2641 acagccttac cactggaggt gtactctaga cactgcctat gtcttttgtc tttaagtaaa
     2701 cagaaacaag tgccttcagt aacttttgtc ccagggttac aaatttgaaa atttgattat
     2761 gctaaaccaa tacaagctta tttaagtgtt tcagttattt tctaatttca agcacaaaca
     2821 aggaaacaaa agttaaatga cacacttagt tactcttgta accagctctg gtactgacca
     2881 ttgtatctcc aggatccttt ctgtaaaccc ttgtcaggag cacctgccac actgtggatg
     2941 acagtgccct caagaatgcc agttaggtta caaagccagg aggatggctt ttggaatttg
     3001 gatatcaagt accgccccca agaagtggct aatgtttgtc ttatatgatt tcatataatt
     3061 gtcacttgta atggcatgat ggagagaaat cttaaatata tattgaacca gaagaagaag
     3121 agttaatcat taaaaatgcc agaagggaag ctcaataaaa tttagattta aataaaactt
     3181 tcatcagtta a
//
