LOCUS       NP_001020734             423 aa            linear   ROD 14-FEB-2025
DEFINITION  pseudouridylate synthase 1 homolog [Rattus norvegicus].
ACCESSION   NP_001020734 XP_222267
VERSION     NP_001020734.1
DBSOURCE    REFSEQ: accession NM_001025563.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 423)
  AUTHORS   Huet,T., Miannay,F.A., Patton,J.R. and Thore,S.
  TITLE     Steroid receptor RNA activator (SRA) modification by the human
            pseudouridine synthase 1 (hPus1p): RNA binding, activity, and
            atomic model
  JOURNAL   PLoS One 9 (4), e94610 (2014)
   PUBMED   24722331
  REMARK    Erratum:[PLoS One. 2015 Mar 25;10(3):e0122147. doi:
            10.1371/journal.pone.0122147.. PMID: 25806947]
            Publication Status: Online-Only
REFERENCE   2  (residues 1 to 423)
  AUTHORS   Czudnochowski,N., Wang,A.L., Finer-Moore,J. and Stroud,R.M.
  TITLE     In human pseudouridine synthase 1 (hPus1), a C-terminal helical
            insert blocks tRNA from binding in the same orientation as in the
            Pus1 bacterial homologue TruA, consistent with their different
            target selectivities
  JOURNAL   J Mol Biol 425 (20), 3875-3887 (2013)
   PUBMED   23707380
REFERENCE   3  (residues 1 to 423)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   4  (residues 1 to 423)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   5  (residues 1 to 423)
  AUTHORS   Sibert,B.S. and Patton,J.R.
  TITLE     Pseudouridine synthase 1: a site-specific synthase without strict
            sequence recognition requirements
  JOURNAL   Nucleic Acids Res 40 (5), 2107-2118 (2012)
   PUBMED   22102571
REFERENCE   6  (residues 1 to 423)
  AUTHORS   Patton,J.R., Bykhovskaya,Y., Mengesha,E., Bertolotto,C. and
            Fischel-Ghodsian,N.
  TITLE     Mitochondrial myopathy and sideroblastic anemia (MLASA): missense
            mutation in the pseudouridine synthase 1 (PUS1) gene is associated
            with the loss of tRNA pseudouridylation
  JOURNAL   J Biol Chem 280 (20), 19823-19828 (2005)
   PUBMED   15772074
REFERENCE   7  (residues 1 to 423)
  AUTHORS   Zhao,X., Patton,J.R., Davis,S.L., Florence,B., Ames,S.J. and
            Spanjaard,R.A.
  TITLE     Regulation of nuclear receptor activity by a pseudouridine synthase
            through posttranscriptional modification of steroid receptor RNA
            activator
  JOURNAL   Mol Cell 15 (4), 549-558 (2004)
   PUBMED   15327771
REFERENCE   8  (residues 1 to 423)
  AUTHORS   Chen,J. and Patton,J.R.
  TITLE     Mouse pseudouridine synthase 1: gene structure and alternative
            splicing of pre-mRNA
  JOURNAL   Biochem J 352 Pt 2 (Pt 2), 465-473 (2000)
   PUBMED   11085940
REFERENCE   9  (residues 1 to 423)
  AUTHORS   Chen,J. and Patton,J.R.
  TITLE     Pseudouridine synthase 3 from mouse modifies the anticodon loop of
            tRNA
  JOURNAL   Biochemistry 39 (41), 12723-12730 (2000)
   PUBMED   11027153
REFERENCE   10 (residues 1 to 423)
  AUTHORS   Chen,J. and Patton,J.R.
  TITLE     Cloning and characterization of a mammalian pseudouridine synthase
  JOURNAL   RNA 5 (3), 409-419 (1999)
   PUBMED   10094309
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000012.1.
            
            On Jul 16, 2005 this sequence version replaced XP_222267.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC161821.1, SRR26643284.16021.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Rattus norvegicus"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="12"
                     /map="12q16"
     Protein         1..423
                     /product="pseudouridylate synthase 1 homolog"
                     /EC_number="5.4.99.12"
                     /note="tRNA pseudouridine synthase A, mitochondrial;
                     tRNA-uridine isomerase I; tRNA pseudouridylate synthase I;
                     tRNA pseudouridine(38-40) synthase; pseudouridylate
                     synthase 1; tRNA pseudouridine synthase 1"
                     /calculated_mol_wt=47384
     Region          32..75
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q4KM92.2)"
     Region          84..336
                     /region_name="PseudoU_synth_PUS1_PUS2"
                     /note="Pseudouridine synthase, PUS1/ PUS2 like; cd02568"
                     /db_xref="CDD:211335"
     Site            order(139..142,291)
                     /site_type="active"
                     /note="probable active site [active]"
                     /db_xref="CDD:211335"
     Region          401..423
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q4KM92.2)"
     Site            411
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9Y606; propagated from
                     UniProtKB/Swiss-Prot (Q4KM92.2)"
     Site            416
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q4KM92.2)"
     Site            422
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q4KM92.2)"
     CDS             1..423
                     /gene="Pus1"
                     /coded_by="NM_001025563.2:265..1536"
                     /db_xref="GeneID:304567"
                     /db_xref="RGD:1311871"
ORIGIN      
        1 mgfprlwaal lrawgrwtar pgprvpglpp magnkvppal pshqpdrkgr ggwvweeteh
       61 pakrvkgged eepprklpkr kivllmaysg kgyhgmqrnl gssqfrtied dlvsalvqag
      121 cipenhgtdm rkmsfqrcar tdkgvsaagq vvslkvwlid dildkinshl pshirilglk
      181 rvtggfnskn kcdartycym lptfafahkd rdvqdesyrl saetlqqvnr llgcykgthn
      241 fhnftsqkgp repsarryil emyceepfvr eglefavikv kgqsfmmhqi rkmvglvvai
      301 vkgyapesvl erswgeekvd vpkapglglv lervhfekyn qrfgsdglhe pldwaqeegk
      361 vtafkeqyiy ptivsterde rsmaqwlntl pihnfsgtal gaddtgakvp sslegsegdg
      421 dtd
//
