LOCUS       NM_001106715            6091 bp    mRNA    linear   ROD 12-NOV-2024
DEFINITION  Rattus norvegicus pumilio RNA-binding family member 2 (Pum2),
            transcript variant 14, mRNA.
ACCESSION   NM_001106715 XM_001068003 XM_216661
VERSION     NM_001106715.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Yu,S., Wang,M., Xie,Y., Wang,B. and Xu,Y.
  TITLE     MiR-495-3p promotes cardiac hypertrophy by targeting Pum2
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 70 (8), 116-120 (2024)
   PUBMED   39262254
  REMARK    GeneRIF: MiR-495-3p promotes cardiac hypertrophy by targeting Pum2.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6091)
  AUTHORS   Yoon,D.S., Choi,Y., Lee,K.M., Ko,E.A., Kim,E.J., Park,K.H. and
            Lee,J.W.
  TITLE     Downregulation of the RNA-binding protein PUM2 facilitates
            MSC-driven bone regeneration and prevents OVX-induced bone loss
  JOURNAL   J Biomed Sci 30 (1), 26 (2023)
   PUBMED   37088847
  REMARK    GeneRIF: Downregulation of the RNA-binding protein PUM2 facilitates
            MSC-driven bone regeneration and prevents OVX-induced bone loss.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6091)
  AUTHORS   Cao,Y., Liu,C., Wang,Q., Wang,W., Tao,E. and Wan,L.
  TITLE     Pum2 mediates Sirt1 mRNA decay and exacerbates
            hypoxia/reoxygenation-induced cardiomyocyte apoptosis
  JOURNAL   Exp Cell Res 393 (1), 112058 (2020)
   PUBMED   32437714
  REMARK    GeneRIF: Pum2 mediates Sirt1 mRNA decay and exacerbates
            hypoxia/reoxygenation-induced cardiomyocyte apoptosis.
REFERENCE   4  (bases 1 to 6091)
  AUTHORS   Martinez,J.C., Randolph,L.K., Iascone,D.M., Pernice,H.F.,
            Polleux,F. and Hengst,U.
  TITLE     Pum2 Shapes the Transcriptome in Developing Axons through Retention
            of Target mRNAs in the Cell Body
  JOURNAL   Neuron 104 (5), 931-946 (2019)
   PUBMED   31606248
  REMARK    GeneRIF: These results reveal that Pum2 shapes axonal
            transcriptomes by preventing the transport of Pumilio-binding
            elements -containing mRNAs into axons
REFERENCE   5  (bases 1 to 6091)
  AUTHORS   Treiber,T., Treiber,N., Plessmann,U., Harlander,S., Daiss,J.L.,
            Eichner,N., Lehmann,G., Schall,K., Urlaub,H. and Meister,G.
  TITLE     A Compendium of RNA-Binding Proteins that Regulate MicroRNA
            Biogenesis
  JOURNAL   Mol Cell 66 (2), 270-284 (2017)
   PUBMED   28431233
REFERENCE   6  (bases 1 to 6091)
  AUTHORS   Fiore,R., Khudayberdiev,S., Christensen,M., Siegel,G.,
            Flavell,S.W., Kim,T.K., Greenberg,M.E. and Schratt,G.
  TITLE     Mef2-mediated transcription of the miR379-410 cluster regulates
            activity-dependent dendritogenesis by fine-tuning Pumilio2 protein
            levels
  JOURNAL   EMBO J 28 (6), 697-710 (2009)
   PUBMED   19197241
  REMARK    GeneRIF: Mef2-induced expression of at least three individual
            miRNAs of the miR379-410 cluster is required for activity-dependent
            dendritic outgrowth of hippocampal neurons.
REFERENCE   7  (bases 1 to 6091)
  AUTHORS   Ginter-Matuszewska,B., Spik,A., Rembiszewska,A., Koyias,C.,
            Kupryjanczyk,J. and Jaruzelska,J.
  TITLE     The SNARE-associated component SNAPIN binds PUMILIO2 and NANOS1
            proteins in human male germ cells
  JOURNAL   Mol Hum Reprod 15 (3), 173-179 (2009)
   PUBMED   19168546
REFERENCE   8  (bases 1 to 6091)
  AUTHORS   Galgano,A., Forrer,M., Jaskiewicz,L., Kanitz,A., Zavolan,M. and
            Gerber,A.P.
  TITLE     Comparative analysis of mRNA targets for human PUF-family proteins
            suggests extensive interaction with the miRNA regulatory system
  JOURNAL   PLoS One 3 (9), e3164 (2008)
   PUBMED   18776931
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 6091)
  AUTHORS   Vessey,J.P., Vaccani,A., Xie,Y., Dahm,R., Karra,D., Kiebler,M.A.
            and Macchi,P.
  TITLE     Dendritic localization of the translational repressor Pumilio 2 and
            its contribution to dendritic stress granules
  JOURNAL   J Neurosci 26 (24), 6496-6508 (2006)
   PUBMED   16775137
REFERENCE   10 (bases 1 to 6091)
  AUTHORS   White,E.K., Moore-Jarrett,T. and Ruley,H.E.
  TITLE     PUM2, a novel murine puf protein, and its consensus RNA-binding
            site
  JOURNAL   RNA 7 (12), 1855-1866 (2001)
   PUBMED   11780640
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH473947.1.
            
            On or before Oct 4, 2007 this sequence version replaced
            XM_216661.4, XM_001068003.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: partial sample support SAMD00132272, SAMD00132283
                              [ECO:0000350]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q14"
     gene            1..6091
                     /gene="Pum2"
                     /note="pumilio RNA-binding family member 2"
                     /db_xref="GeneID:298874"
                     /db_xref="RGD:1311024"
     exon            1..55
                     /gene="Pum2"
                     /inference="alignment:Splign:2.1.0"
     exon            56..124
                     /gene="Pum2"
                     /inference="alignment:Splign:2.1.0"
     CDS             74..3259
                     /gene="Pum2"
                     /note="isoform 14 is encoded by transcript variant 14;
                     pumilio 2"
                     /codon_start=1
                     /product="pumilio homolog 2 isoform 14"
                     /protein_id="NP_001100185.1"
                     /db_xref="GeneID:298874"
                     /db_xref="RGD:1311024"
                     /translation="MNHDFQALALESRGMGELLPTKKFWEPDDSTKDGQKGIFLGDDE
                     WRETAWGTSHHSMSQPIMVQRRSGQSFHGNSEVNAILSPRSESGGLGVSMVEYVLSSS
                     PADKLDSRFRKGTFGTRDAETDGPEKGDQKGKASPFEEDQNRDLKQDDEDSKINGRGL
                     PNGMDADCKDFNRTPGSRQASPTEVVERLGPSTNPPEGLGPLPNPTANKPLVEEFSNP
                     ETQNLDAMDQVGLDSLQFDYPGNQVPMDSSGATVGLFDYNSQQQLFQRTNALTVQQLT
                     AAQQQQYALAAAQQPHIAGVFSAGLAPAAFVPNPYIISAAPPGTDPYTAAGLAAAATL
                     AGPAVVPPQYYGVPWGVYPANLFQQQAAAAASNTANQQAASQAQPGQQQVLRPGAGQR
                     PITPSQGQQGQQAESLAAAAAANPTLAFGQSLAAGMPGYQVLAPTAYYDQTGALVVGP
                     GARTGLGAPVRLMAPTPVLISSAAAQAAAAAAAAGGTANSLTGSTNGLFRPIGTQPPQ
                     QQQQQQPSTNLQSNSFYGSSSLTNSSQSSSLFSHGPGQPGSASLGFGSGSSLGAAIGS
                     ALSGFGSSVGSSASSSATRRESLSTSSDLYKRSSSSLAPIGQPFYNSLGFSSSPSPIG
                     MPLPSQTPGHSLTPPPSLSSHGSSSRGLTNGSGRYISAAPGAEAKYRSASSTSSLFSS
                     SSQLFPPSRLRYNRSDIMPSGRSRLLEDFRNNRFPNLQLRDLMGHIVEFSQDQHGSRF
                     IQQKLERATPAERQMVFNEIVQAAYQLMTDVFGNYVIQKFFEFGSLDQKLALANRIRG
                     HVLPLALQMYGCRVIQKALESISSDQQSEMVKELDGHVLKCVKDQNGNHVVQKCIECV
                     QPQSLQFIIDAFKGQVFVLSTHPYGCRVIQRILEHCTAEQTLPILEELHQHTEQLVQD
                     QYGNYVIQHVLEHGRPEDKSKIVSEIRGKVLALSQHKFASNVVEKCVTHASRAERALL
                     IDEVCCQNDGPHSALYTMMKDQYANYVVQKMIDMAEPAQRKIIMHKIRPHITTLRKYT
                     YGKHILAKLEKYYLKNSPDLGPIGGPPNGML"
     exon            125..233
                     /gene="Pum2"
                     /inference="alignment:Splign:2.1.0"
     exon            234..421
                     /gene="Pum2"
                     /inference="alignment:Splign:2.1.0"
     exon            422..588
                     /gene="Pum2"
                     /inference="alignment:Splign:2.1.0"
     exon            589..859
                     /gene="Pum2"
                     /inference="alignment:Splign:2.1.0"
     exon            860..953
                     /gene="Pum2"
                     /inference="alignment:Splign:2.1.0"
     exon            954..1079
                     /gene="Pum2"
                     /inference="alignment:Splign:2.1.0"
     exon            1080..1222
                     /gene="Pum2"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..1361
                     /gene="Pum2"
                     /inference="alignment:Splign:2.1.0"
     exon            1362..1505
                     /gene="Pum2"
                     /inference="alignment:Splign:2.1.0"
     exon            1506..1796
                     /gene="Pum2"
                     /inference="alignment:Splign:2.1.0"
     exon            1797..2021
                     /gene="Pum2"
                     /inference="alignment:Splign:2.1.0"
     exon            2022..2289
                     /gene="Pum2"
                     /inference="alignment:Splign:2.1.0"
     exon            2290..2419
                     /gene="Pum2"
                     /inference="alignment:Splign:2.1.0"
     exon            2420..2548
                     /gene="Pum2"
                     /inference="alignment:Splign:2.1.0"
     exon            2549..2686
                     /gene="Pum2"
                     /inference="alignment:Splign:2.1.0"
     exon            2687..2812
                     /gene="Pum2"
                     /inference="alignment:Splign:2.1.0"
     exon            2813..2934
                     /gene="Pum2"
                     /inference="alignment:Splign:2.1.0"
     exon            2935..3127
                     /gene="Pum2"
                     /inference="alignment:Splign:2.1.0"
     exon            3128..6091
                     /gene="Pum2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggttcccgca gtctggaaga agctcggtgg acccagagaa acgtgcgagt tggaagtttg
       61 acctgtggat gaaatgaatc atgattttca agctcttgca ttagaatctc ggggaatggg
      121 agagcttttg cctaccaaaa aattttggga acctgatgat tcaacaaaag atggacaaaa
      181 aggcatattt cttggggatg atgaatggag agagactgca tggggaactt ctcatcattc
      241 aatgtcccag cctattatgg tacagagaag atctggacag agttttcatg gaaacagtga
      301 agtaaatgca attctttctc cgcgctcaga aagtggaggc cttggcgtga gcatggtaga
      361 atatgtatta agttcctctc ctgctgataa attggattct cgatttagga agggaacttt
      421 tggtactaga gatgctgaaa cagatggacc tgagaaagga gatcaaaaag gcaaggcttc
      481 tccatttgag gaggaccaaa acagagatct taaacaagat gatgaggact ctaaaataaa
      541 tggcagaggt ttgccaaatg gaatggatgc cgattgcaaa gattttaatc gtactccggg
      601 aagtcgtcaa gcctctccaa ctgaagtagt tgagcgcctt ggccctagta ctaatccccc
      661 agaaggattg ggtcctcttc ctaatccgac agcgaataaa ccacttgttg aagaattttc
      721 aaatcctgaa actcagaatc tggatgcaat ggaccaagtt ggtctggatt ccttacagtt
      781 tgactatcct ggtaatcagg tacccatgga ttcttcagga gctactgtag gcctttttga
      841 ctacaattcc caacagcagc tctttcagag gactaatgca ctaacagttc agcagttaac
      901 tgctgctcaa cagcagcagt atgcattagc agcagctcag cagccgcata tagctggtgt
      961 attctcagca ggccttgctc cagctgcatt tgtgccaaat ccatatatta ttagtgctgc
     1021 tcctccaggg accgacccgt atactgcagc aggattggct gcagcagcaa cattagcagg
     1081 tccagcagtg gttccacctc agtattatgg tgttccatgg ggggtgtatc cagccaattt
     1141 atttcagcaa caagctgcag ctgcggcaag caacacagcc aatcagcaag cagcatcaca
     1201 agctcagcct ggacagcagc aggttcttcg tcctggagca ggtcaacgtc ctattactcc
     1261 cagtcagggc caacaagggc agcaagcaga gtcacttgca gcagccgcag ctgcaaaccc
     1321 aactttggct tttggtcaga gtcttgctgc aggcatgcca ggctatcaag tactagctcc
     1381 aactgcctat tatgatcaga ctggtgcctt agtggttggc cctggagcaa gaactggcct
     1441 tggagctcca gtacgattaa tggctccaac acctgtctta attagttcag cagcagcaca
     1501 agctgcagca gcagcagcag cagctggagg aactgcaaat agtcttacag gcagcacaaa
     1561 cggtctgttt cggccgattg gcactcagcc accgcagcag cagcagcagc agcagccaag
     1621 cactaacctg cagtctaatt cgttttatgg gagcagctct ttgactaaca gctcccagag
     1681 cagctcttta ttttctcacg gacctggcca acctggaagt gcatctcttg gctttggaag
     1741 tggtagctct ttgggagccg ctataggctc agctctcagt ggatttggct catcagttgg
     1801 cagttctgca agtagtagtg ccacaaggag agagtctcta tctactagct ctgacttgta
     1861 caaaagatct agtagcagcc tagcacccat agggcaacca ttttacaata gtctgggatt
     1921 ttcctcctct ccaagtccaa taggcatgcc tctgccaagc caaactccag gacattcact
     1981 tacgccaccg ccatcacttt catcacatgg atcctcatcc agaggcctga caaatggtag
     2041 tggtcggtac atctctgcag cacctggagc ggaagcaaag taccggagcg cttccagcac
     2101 ttccagcctg ttcagctcca gcagccagct ctttcctccc tctcggctcc gctataacag
     2161 atctgatatc atgccctctg gccgcagcag gctattggaa gatttcagga acaaccgctt
     2221 ccccaacctc cagcttagag acttgatggg acatatagtc gagttttctc aagaccagca
     2281 tggctctaga ttcatacagc agaagctaga gagagctact ccagctgagc gacagatggt
     2341 atttaatgaa attgtacagg ctgcctatca attaatgaca gatgtttttg gaaactatgt
     2401 tatacagaag ttttttgagt ttggaagttt ggatcagaaa ttagccctgg ctaatcgtat
     2461 tcgtggtcat gttctaccat tagccttgca gatgtatggc tgccgtgtta ttcaaaaagc
     2521 attagaatct atttcttctg atcagcagag tgaaatggtc aaggaacttg atggtcatgt
     2581 acttaaatgt gtgaaagatc agaatggaaa ccatgttgta cagaagtgta ttgaatgtgt
     2641 tcaaccgcag tcactgcagt tcatcatcga tgctttcaaa ggacaagtat ttgtgctttc
     2701 aacccatcct tacggctgca gagtcattca gcggatctta gagcactgta ctgcagagca
     2761 gactttaccc atcttagaag agcttcacca acacacagag cagttggtac aggatcagta
     2821 tggcaattat gttattcagc atgtactgga acatggtcgg cctgaagaca agagcaaaat
     2881 tgtttctgaa atcagaggaa aggtcttagc cctgagtcaa cacaaatttg ccagcaatgt
     2941 agtagaaaag tgtgttactc atgcctcacg tgctgagaga gctttactga ttgatgaggt
     3001 ctgctgtcag aatgatggtc ctcacagtgc cttatacacc atgatgaagg atcaatatgc
     3061 caactatgtg gttcagaaga tgattgatat ggctgagcct gctcagagaa agataatcat
     3121 gcacaagatt cgacctcaca tcactactct tcgcaaatac acatatggga agcatatact
     3181 ggccaagttg gaaaaatact atttgaaaaa cagcccagat ctagggccta ttggaggacc
     3241 accaaatgga atgctgtaag gaaaaacgag aagatgattt aaccatgttc aaagaatggg
     3301 ttttctttgt gaattatcaa aacacaattc aactatgaat ctttgatttt ttttttaaag
     3361 caaaactatt tattgacttt cttcatccat ttgtaaattt ttaaggttct tgtgtatatt
     3421 tagggggtgg gggtggtgaa ttataaatta tattcaaccc tgagtggaga cctatcagat
     3481 tggatttctg gtgaagcaca gaatgcctgt atatgatgta actgtgtccc ccccacaaaa
     3541 aaaaaaaagg aaaaaaagtt gtcgcatatt ttgtaaattt ataccttgta aagtcacaaa
     3601 aaatagtttt taaagggaaa agtacaatat tttaataaac tggcttgcag tctggtaggt
     3661 ctacaacccc atagcacaac aggtttatag agatgtatat agaattatag ttcttatttt
     3721 tttcctttgt gtgaagcctt ttataacaga ataacaatca actgcataaa tattattaat
     3781 attttaaaaa gaaagttaag ttgtattttg gtaattcaca aactatcatg caaagaaaaa
     3841 ctcagaaaga cagaaaagaa taaaatgatt tgagataaca ttatttacag tggatgtggt
     3901 tttaataaaa ttactgccct ataatgtctc tgacaatgta cagaattgta tatacttata
     3961 attgtaatga gttagaaaca aaatcatggt ggcacttcca ttcagttaaa actgcactga
     4021 atgaaaagtt aaatttcaaa ctaacttttc agtttggttt gcaacaagaa tggatggaga
     4081 tttatatttt gttctaatta tagaattact agactgttga ggaagtgatg gtttatcctg
     4141 cgttttcata gtagatacat aaagtaggac ccgatagagg tgagagaggc cactgagaag
     4201 tgaactgata gctcagcatt tacgcatgac tgcatattca gagagctttt ttttcccttc
     4261 ctataaataa acacattgtg tgtgtagggg tagatgtaag tttacaattt gaaagcagat
     4321 cttgtttcag tgtttgttat gaaagccttg ctaatttcgt agtgactttc cttggttgta
     4381 caggtgtaca tttgtaaacc ttcatgctgt aaatggaatc tgttttctct ctttggaaaa
     4441 cacttgcatt ttagtgtaca tttccatccc tgccctcttt aacctggcaa tatagtgttg
     4501 tataatgtaa atttatttct ccaaattgag agtgattttt aaaaattttt tatctttata
     4561 tggtttcaga agtatgaacc agctttcttt ttattattgt gggaaacatt ttgttttata
     4621 acatagttgt tgactctgtt aataatggac atgctaggat ctggatcact ttcaattgaa
     4681 gtcagggtat tgtgcatagt gagtagaaag tgttggactg agattgatta ccacagaggc
     4741 caatgctttt ccatctttaa atgtgatgtg acttttttct ttgtacagaa gagtactgta
     4801 tttttgaata gcctactccc aagtaagagc agatctgtat gataacattt ttcctctgga
     4861 cataagacat tataacagta acatgatgta catttacaac gcggccttat gtacatttcc
     4921 caacaatctt tttaaggcaa aattgtgacc atatgtgtat aattaaaatc gtttttaatc
     4981 ctttgcctat gaaaatattt tggaaaaaat tgctttgtat attcagtttc tgaaagataa
     5041 agaaagtgct ttgtattttg ttgaagtcag tattttgtat aaactttatg ttgacccact
     5101 tacgtacatt cagtgctgaa aactgaaatg accatgcaag ttctggcagg tgaattgtag
     5161 aagggattct tacttgaggt ctgcagaaga aaaactatga gagcacaata ttgagtatga
     5221 agattccatc cttttgttac tcagatcatg aaccttaaaa tttggcatgg agtttggggt
     5281 gccaggagtc tttcacattg atattgagtt actgtagctt tctaacccaa cattccttgg
     5341 tgaggccatt aagtctggcc acgtgtcacc attctcggtc acactggttc atcagcatgt
     5401 ccctgtcaca gtagcagtat tctctctcat gtagaatgac tctctttccc ctttttatta
     5461 ctttgttttg ccagcttcag agtgtcttgg ccctgggttg aatttaactt cccttcatca
     5521 gcccatggtt tcgggttaaa gctgtcagtg ttagagattg gttacagctc ttcttgcaga
     5581 aatgcagccc attctggtgt gtcctcacct ttccctttgg atgtcccgac agctgtcact
     5641 cggctcagaa ggtggcaagt cctagagaaa gttatttgga actgaatcct cctctagcag
     5701 tcctgattca tacctcccag tgctgacaac ccagtcctcc tgatattttt aggcatattc
     5761 tagaaaaata cctgttcgtg tagaaaattt atgatctgcc aattttggtg cccaccccga
     5821 ttctaccttc tagtatgttg gcatggaact ttagaaaaat cggacatttc agaataaaga
     5881 catctgattg ggcagaatct gcttaggctt tacttttgag tgtctctttt tgatatagat
     5941 tgtttctgga ccagtttgtc taagtcctgg ctcttattgg ctcatatgaa atgtcatctt
     6001 cactttgtat attatgtata aattagaaaa tgaaacgtgt gaataacatt gtatgaaata
     6061 aacctggtct tgtgtttttc tctagataaa a
//
