LOCUS       NM_012636               1087 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus parathyroid hormone-like hormone (Pthlh), mRNA.
ACCESSION   NM_012636
VERSION     NM_012636.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Amaya,Y. and Nakai,T.
  TITLE     Localization and ER membrane insertion of parathyroid
            hormone-related protein analyzed without effects of reporter
            proteins
  JOURNAL   FEBS Lett 594 (2), 383-392 (2020)
   PUBMED   31471966
  REMARK    GeneRIF: Mutational analyses including insertion of a glycosylation
            site for the tagged PTHrP revealed that the evolutionarily
            conserved regions in the signal peptide and the pro-region
            facilitate the redirection of ppPTHrP from the secretory pathway to
            the nuclear targeting pathway.
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   He,S., Tang,J., Diao,N., Liao,Y., Shi,J., Xu,X., Xie,F. and Bai,L.
  TITLE     Parathyroid hormone-related protein activates HSCs via hedgehog
            signalling during liver fibrosis development
  JOURNAL   Artif Cells Nanomed Biotechnol 47 (1), 1984-1994 (2019)
   PUBMED   31311343
  REMARK    GeneRIF: PTHLH-mediated PTH1R-PKC theta; pathway activation is a
            key event in the profibrotic Hh-dependent activation of HSCs
REFERENCE   3  (bases 1 to 1087)
  AUTHORS   Liu,C., Shao,G., Lu,Y., Xue,M., Liang,F., Zhang,Z. and Bai,L.
  TITLE     Parathyroid Hormone-Related Protein (1-40) Enhances Calcium Uptake
            in Rat Enterocytes Through PTHR1 Receptor and Protein Kinase
            Calpha/beta Signaling
  JOURNAL   Cell Physiol Biochem 51 (4), 1695-1709 (2018)
   PUBMED   30504697
  REMARK    GeneRIF: PTHrP (1-40) stimulates calcium uptake via PTHR1 receptor
            and PKC alpha/beta signaling pathway in rat enterocytes, and
            calcium transporters TRPV6 and CaBP-D9k are necessary for this
            stimulatory effect.
REFERENCE   4  (bases 1 to 1087)
  AUTHORS   Sun,W., Wu,J., Huang,L., Liu,H., Wang,R., Karaplis,A., Goltzman,D.
            and Miao,D.
  TITLE     PTHrP Nuclear Localization and Carboxyl Terminus Sequences Modulate
            Dental and Mandibular Development in Part via the Action of p27
  JOURNAL   Endocrinology 157 (4), 1372-1384 (2016)
   PUBMED   26859332
REFERENCE   5  (bases 1 to 1087)
  AUTHORS   Amaya,Y., Nakai,T. and Miura,S.
  TITLE     Evolutionary well-conserved region in the signal peptide of
            parathyroid hormone-related protein is critical for its dual
            localization through the regulation of ER translocation
  JOURNAL   J Biochem 159 (4), 393-406 (2016)
   PUBMED   26538570
  REMARK    GeneRIF: These results indicate that the well-conserved region in
            the signal peptide plays an essential role in the dual localization
            of PTHrP through ER targeting and/or the membrane translocation.
REFERENCE   6  (bases 1 to 1087)
  AUTHORS   Karaplis,A.C., Luz,A., Glowacki,J., Bronson,R.T., Tybulewicz,V.L.,
            Kronenberg,H.M. and Mulligan,R.C.
  TITLE     Lethal skeletal dysplasia from targeted disruption of the
            parathyroid hormone-related peptide gene
  JOURNAL   Genes Dev 8 (3), 277-289 (1994)
   PUBMED   8314082
REFERENCE   7  (bases 1 to 1087)
  AUTHORS   Karaplis,A.C., Yasuda,T., Hendy,G.N., Goltzman,D. and Banville,D.
  TITLE     Gene-encoding parathyroid hormone-like peptide: nucleotide sequence
            of the rat gene and comparison with the human homologue
  JOURNAL   Mol Endocrinol 4 (3), 441-446 (1990)
   PUBMED   2342478
REFERENCE   8  (bases 1 to 1087)
  AUTHORS   Yasuda,T., Banville,D., Rabbani,S.A., Hendy,G.N. and Goltzman,D.
  TITLE     Rat parathyroid hormone-like peptide: comparison with the human
            homologue and expression in malignant and normal tissue
  JOURNAL   Mol Endocrinol 3 (3), 518-525 (1989)
   PUBMED   2747658
REFERENCE   9  (bases 1 to 1087)
  AUTHORS   Thiede,M.A. and Rodan,G.A.
  TITLE     Expression of a calcium-mobilizing parathyroid hormone-like peptide
            in lactating mammary tissue
  JOURNAL   Science 242 (4876), 278-280 (1988)
   PUBMED   3175653
REFERENCE   10 (bases 1 to 1087)
  AUTHORS   Chou,C.H., Fritz,R.B., Chou,F.C. and Kibler,R.F.
  TITLE     The immune response of Lewis rats to peptide 68-88 of guinea pig
            myelin basic protein. I. T cell determinants
  JOURNAL   J Immunol 123 (4), 1540-1543 (1979)
   PUBMED   90087
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            M31603.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M31603.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760384, SAMN06621351
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q44"
     gene            1..1087
                     /gene="Pthlh"
                     /gene_synonym="PLP"
                     /note="parathyroid hormone-like hormone"
                     /db_xref="GeneID:24695"
                     /db_xref="RGD:3441"
     exon            1..49
                     /gene="Pthlh"
                     /gene_synonym="PLP"
                     /inference="alignment:Splign:2.1.0"
     exon            50..175
                     /gene="Pthlh"
                     /gene_synonym="PLP"
                     /inference="alignment:Splign:2.1.0"
     CDS             75..608
                     /gene="Pthlh"
                     /gene_synonym="PLP"
                     /note="parathyroid hormone-related protein; PTHrP; PTH-rP;
                     parathyroid hormone-like protein; parathyroid-like
                     peptide; parathyroid hormone-like peptide"
                     /codon_start=1
                     /product="parathyroid hormone-related protein
                     preproprotein"
                     /protein_id="NP_036768.1"
                     /db_xref="GeneID:24695"
                     /db_xref="RGD:3441"
                     /translation="MLRRLVQQWSVLVFLLSYSVPSRGRSVEGLGRRLKRAVSEHQLL
                     HDKGKSIQDLRRRFFLHHLIAEIHTAEIRATSEVSPNSKPAPNTKNHPVRFGSDDEGR
                     YLTQETNKVETYKEQPLKTPGKKKKGKPGKRREQEKKKRRTRSAWPGTTGSGLLEDPQ
                     PHTSPTSTSLEPSSRTH"
     exon            176..598
                     /gene="Pthlh"
                     /gene_synonym="PLP"
                     /inference="alignment:Splign:2.1.0"
     exon            599..1087
                     /gene="Pthlh"
                     /gene_synonym="PLP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1057..1062
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pthlh"
                     /gene_synonym="PLP"
     polyA_site      1087
                     /gene="Pthlh"
                     /gene_synonym="PLP"
ORIGIN      
        1 cctgtgcggt ttgagagagg cacagttgga ggcgctgatt cctacaaaag tccccagagc
       61 cagcgagcgg cacgatgctg cggaggctgg ttcagcagtg gagcgtcctg gtgttcctgc
      121 tcagctactc cgtgccctcc cgcgggcgct cggtggaggg gcttggtcgc aggctaaaac
      181 gcgcggtgtc tgagcaccag ctactgcatg acaagggcaa gtccatccaa gacttgcgcc
      241 gccgtttctt cctccaccat ctgattgcgg agatccacac agctgaaatc agagctacct
      301 cggaggtgtc ccctaactcc aaacctgctc ccaacaccaa aaaccaccct gtgcggtttg
      361 ggtcagacga cgagggcaga tacctaactc aggaaaccaa caaggtggag acgtacaaag
      421 agcagccact caagacgccc gggaagaaga agaaaggcaa gccggggaaa cgcagagaac
      481 aggagaaaaa aaagcgaagg actcggtctg cctggccagg cacaactggg agtggcctgc
      541 ttgaggaccc ccagccccac acctccccga cctcgacctc cctggagccc agctcaagga
      601 cgcattgaaa ctttcaccga agatcttcca aggacacgtt acaggattct gtaatagtaa
      661 acatatggaa agtattagac atatttattg cctgtacata ctgtaaatgc attgggatca
      721 aactgtctcc ccaggaaact gcacatgggt catgtgaata tttttccttc tttttttttt
      781 tttgccaagg ctaatccaat tattactgtc actgttacca taatttattt tgtcaactga
      841 tgtatttatt tgtaaatgta tcttggtgct gctgactctg tttttttgta acataatgca
      901 ctttaggtat acatatcaag tatgtcgatg aatttaacac gaaaaagtgt ctctattttg
      961 tggttcattt tgatgagttc tgaaatataa ttatctagac tgatctccct ctgcgcatgt
     1021 aaaaatggca gtattttaaa tttgtaaaga atgtctaata aaatataatc taattacacc
     1081 gtgactt
//
