LOCUS       NM_013013               2576 bp    mRNA    linear   ROD 29-JUL-2024
DEFINITION  Rattus norvegicus prosaposin (Psap), transcript variant 1, mRNA.
ACCESSION   NM_013013
VERSION     NM_013013.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Wu,K.J., Hung,T.W., Wang,Y.S., Chen,Y.H., Bae,E.K. and Yu,S.J.
  TITLE     Prosaposin PS18 reduces dopaminergic neurodegeneration in a
            6-hydroxydopamine rat model of Parkinson's disease
  JOURNAL   Sci Rep 13 (1), 8148 (2023)
   PUBMED   37208379
  REMARK    GeneRIF: Prosaposin PS18 reduces dopaminergic neurodegeneration in
            a 6-hydroxydopamine rat model of Parkinson's disease.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Carvelli,L., Aguilera,A.C., Zyla,L., Pereyra,L.L., Morales,C.R.,
            Hermo,L. and Sosa,M.A.
  TITLE     Castration causes an increase in lysosomal size and upregulation of
            cathepsin D expression in principal cells along with increased
            secretion of procathepsin D and prosaposin oligomers in adult rat
            epididymis
  JOURNAL   PLoS One 16 (4), e0250454 (2021)
   PUBMED   33914781
  REMARK    GeneRIF: Castration causes an increase in lysosomal size and
            upregulation of cathepsin D expression in principal cells along
            with increased secretion of procathepsin D and prosaposin oligomers
            in adult rat epididymis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2576)
  AUTHORS   Kunihiro,J., Nabeka,H., Wakisaka,H., Unuma,K., Khan,M.S.I.,
            Shimokawa,T., Islam,F., Doihara,T., Yamamiya,K., Saito,S.,
            Hamada,F. and Matsuda,S.
  TITLE     Prosaposin and its receptors GRP37 and GPR37L1 show increased
            immunoreactivity in the facial nucleus following facial nerve
            transection
  JOURNAL   PLoS One 15 (12), e0241315 (2020)
   PUBMED   33259479
  REMARK    GeneRIF: Prosaposin and its receptors GRP37 and GPR37L1 show
            increased immunoreactivity in the facial nucleus following facial
            nerve transection.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2576)
  AUTHORS   Islam,F., Khan,M.S.I., Nabeka,H., Shimokawa,T., Yamamiya,K. and
            Matsuda,S.
  TITLE     Age- and sex-associated changes in prosaposin and its receptors in
            the lacrimal glands of rats
  JOURNAL   Histol Histopathol 35 (1), 69-81 (2020)
   PUBMED   31215019
  REMARK    GeneRIF: Age- and sex-associated changes in prosaposin and its
            receptors in the lacrimal glands of rats.
REFERENCE   5  (bases 1 to 2576)
  AUTHORS   Islam,F., Khan,M.S.I., Nabeka,H., Saito,S., Li,X., Shimokawa,T.,
            Yamamiya,K., Kobayashi,N. and Matsuda,S.
  TITLE     Prosaposin and its receptors are differentially expressed in the
            salivary glands of male and female rats
  JOURNAL   Cell Tissue Res 373 (2), 439-457 (2018)
   PUBMED   29656342
  REMARK    GeneRIF: The present findings indicate that PSAP and its receptors,
            GPR37 and GPR37L1, are expressed in the major salivary glands of
            rats and their immunoreactivities differ considerably with age and
            sex.
REFERENCE   6  (bases 1 to 2576)
  AUTHORS   Oya,Y., Nakayasu,H., Fujita,N., Suzuki,K. and Suzuki,K.
  TITLE     Pathological study of mice with total deficiency of sphingolipid
            activator proteins (SAP knockout mice)
  JOURNAL   Acta Neuropathol 96 (1), 29-40 (1998)
   PUBMED   9678511
REFERENCE   7  (bases 1 to 2576)
  AUTHORS   Morales,C.R., El-Alfy,M., Zhao,Q. and Igdoura,S.A.
  TITLE     Expression and tissue distribution of rat sulfated glycoprotein-1
            (prosaposin)
  JOURNAL   J Histochem Cytochem 44 (4), 327-337 (1996)
   PUBMED   8601692
REFERENCE   8  (bases 1 to 2576)
  AUTHORS   Morales,C.R., el-Alfy,M., Zhao,Q. and Igdoura,S.
  TITLE     Molecular role of sulfated glycoprotein-1 (SGP-1/prosaposin) in
            Sertoli cells
  JOURNAL   Histol Histopathol 10 (4), 1023-1034 (1995)
   PUBMED   8573994
  REMARK    Review article
REFERENCE   9  (bases 1 to 2576)
  AUTHORS   Hiraiwa,M., Soeda,S., Kishimoto,Y. and O'Brien,J.S.
  TITLE     Binding and transport of gangliosides by prosaposin
  JOURNAL   Proc Natl Acad Sci U S A 89 (23), 11254-11258 (1992)
   PUBMED   1454804
REFERENCE   10 (bases 1 to 2576)
  AUTHORS   Collard,M.W., Sylvester,S.R., Tsuruta,J.K. and Griswold,M.D.
  TITLE     Biosynthesis and molecular cloning of sulfated glycoprotein 1
            secreted by rat Sertoli cells: sequence similarity with the
            70-kilodalton precursor to sulfatide/GM1 activator
  JOURNAL   Biochemistry 27 (12), 4557-4564 (1988)
   PUBMED   3048385
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CK365248.1, M19936.1, DY313840.1, BC061759.1 and CO558861.1.
            
            On Jun 13, 2010 this sequence version replaced NM_013013.1.
            
            Transcript Variant: This variant (1) lacks an exon in the coding
            region, compared to variant 2, resulting in a shorter protein
            (isoform A), compared to isoform B.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ217536.1, M19936.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-296               CK365248.1         1-296
            297-1174            M19936.1           247-1124
            1175-1855           DY313840.1         40-720
            1856-2331           BC061759.1         1787-2262
            2332-2576           CO558861.1         1-245               c
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="20"
                     /map="20q11"
     gene            1..2576
                     /gene="Psap"
                     /gene_synonym="SGP-1; SGP1A"
                     /note="prosaposin"
                     /db_xref="GeneID:25524"
                     /db_xref="RGD:3423"
     exon            1..117
                     /gene="Psap"
                     /gene_synonym="SGP-1; SGP1A"
                     /inference="alignment:Splign:2.1.0"
     CDS             78..1742
                     /gene="Psap"
                     /gene_synonym="SGP-1; SGP1A"
                     /note="isoform A preproprotein is encoded by transcript
                     variant 1; sulfated glycoprotein 1; prosaposin (sulfated
                     glycoprotein, sphingolipid hydrolase activator)"
                     /codon_start=1
                     /product="prosaposin isoform A preproprotein"
                     /protein_id="NP_037145.2"
                     /db_xref="GeneID:25524"
                     /db_xref="RGD:3423"
                     /translation="MYALALLASLLVTALTSPVQDPKICSGGSAVVCRDVKTAVDCRA
                     VKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATEEEILHYLEKTCAWIHDSSL
                     SASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQSLQEYLAEQNQRQLESNKIPE
                     VDLARVVAPFMSNIPLLLYPQDRPRSQPQPKANEDVCQDCMKLVTDIQTAVRTNSSFV
                     QGLVDHVKEDCDRLGPGVSDICKNYVDQYSEVAVQMMMHMQPKEICVMVGFCDEVKRV
                     PMRTLVPATEAIKNILPALELTDPYEQDVIQAQNVIFCQVCQLVMRKLSELIINNATE
                     ELLIKGLSKACSLLPAPASTKCQEVLVTFGPSLLDVLMHEVNPNFLCGVISLCSANPN
                     LVGTLEQPAAAIVSALPKEPAPPKQPEEPKQSALRAHVPPQKNGGFCEVCKKLVIYLE
                     HNLEKNSTKEEILAALEKGCSFLPDPYQKQCDEFVAEYEPLLLEILVEVMDPSFVCSK
                     IGVCPSAYKLLLGTEKCVWGPGYWCQNMETAARCNAVDHCKRHVWN"
     exon            118..251
                     /gene="Psap"
                     /gene_synonym="SGP-1; SGP1A"
                     /inference="alignment:Splign:2.1.0"
     exon            252..326
                     /gene="Psap"
                     /gene_synonym="SGP-1; SGP1A"
                     /inference="alignment:Splign:2.1.0"
     exon            327..452
                     /gene="Psap"
                     /gene_synonym="SGP-1; SGP1A"
                     /inference="alignment:Splign:2.1.0"
     exon            453..650
                     /gene="Psap"
                     /gene_synonym="SGP-1; SGP1A"
                     /inference="alignment:Splign:2.1.0"
     exon            651..794
                     /gene="Psap"
                     /gene_synonym="SGP-1; SGP1A"
                     /inference="alignment:Splign:2.1.0"
     exon            795..851
                     /gene="Psap"
                     /gene_synonym="SGP-1; SGP1A"
                     /inference="alignment:Splign:2.1.0"
     exon            852..983
                     /gene="Psap"
                     /gene_synonym="SGP-1; SGP1A"
                     /inference="alignment:Splign:2.1.0"
     exon            984..1079
                     /gene="Psap"
                     /gene_synonym="SGP-1; SGP1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1080..1359
                     /gene="Psap"
                     /gene_synonym="SGP-1; SGP1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1360..1517
                     /gene="Psap"
                     /gene_synonym="SGP-1; SGP1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1518..1598
                     /gene="Psap"
                     /gene_synonym="SGP-1; SGP1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1599..1706
                     /gene="Psap"
                     /gene_synonym="SGP-1; SGP1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1707..2560
                     /gene="Psap"
                     /gene_synonym="SGP-1; SGP1A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2540..2545
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Psap"
                     /gene_synonym="SGP-1; SGP1A"
     polyA_site      2560
                     /gene="Psap"
                     /gene_synonym="SGP-1; SGP1A"
ORIGIN      
        1 ggcggggttg gggctagatt tatatgcgcc tggtcagctg acgatctgca ttgcagcctg
       61 cggagtgaag cggcgccatg tatgctctcg ctctcctcgc cagccttctg gtcaccgctc
      121 tgaccagccc tgtccaggac ccgaagatct gctctggggg ctcagcagta gtgtgcagag
      181 atgtgaagac ggcggtggac tgtagggctg tgaagcactg ccagcagatg gtctggagca
      241 agcccacagc aaaatccctt ccttgtgaca tatgcaaaac ggttgtcacc gaagctggga
      301 acttgctgaa agataatgct actgaggagg agatcctcca ttacctggag aagacctgtg
      361 cgtggattca tgactccagc ctgtcagcct cttgcaagga ggtggttgac tcttacctgc
      421 ctgtcatcct ggacatgatt aagggggaga tgagcaaccc cggggaagtg tgctctgcgc
      481 tcaacctctg ccagtccctt caggagtact tggccgagca aaaccagaga cagctggagt
      541 ccaacaagat cccggaggtg gacctggccc gcgtggttgc ccccttcatg tccaacatcc
      601 ctctcctgct gtaccctcag gatcggcctc gcagccagcc gcagcccaag gctaacgagg
      661 acgtctgcca ggactgtatg aagttggtga ctgacatcca gactgctgtg aggaccaact
      721 ccagctttgt ccagggcttg gtggaccacg tcaaggagga ttgtgaccgc ctcgggccag
      781 gcgtgtctga catatgcaag aactatgttg accagtattc tgaggtcgcc gtccagatga
      841 tgatgcacat gcaacccaag gaaatctgtg tgatggttgg cttctgtgat gaggtcaaga
      901 gagtgccaat gaggactctg gtccccgcca ctgaggccat caagaatatc ctccctgccc
      961 tggagctgac ggacccctat gagcaggatg tgatccaggc ccaaaatgtg attttctgcc
     1021 aagtttgtca gcttgtgatg cgcaagttgt ctgaactgat tatcaacaat gccactgagg
     1081 aacttctaat taaaggtttg agtaaagcct gctccctgct ccctgctcct gcttcaacca
     1141 agtgccagga agtgctggta acatttggcc cctctctgtt ggacgtcctc atgcatgagg
     1201 tgaacccgaa ctttctgtgc ggtgtgatca gcctctgttc tgccaacccg aatttggtgg
     1261 ggacccttga acaacctgca gcagccattg tatctgcact gcccaaagag cctgcaccgc
     1321 caaaacagcc tgaagagccc aagcagtctg cattgcgcgc ccatgtgcct cctcagaaga
     1381 acggggggtt ctgtgaggtg tgcaagaagc tggtcatcta tttggaacat aacctggaga
     1441 aaaacagcac caaggaggag atcctggctg cacttgagaa gggctgcagc ttcctgccag
     1501 acccttacca gaagcagtgt gatgaatttg tggctgagta tgagccctta ctgctggaaa
     1561 tccttgtgga ggtgatggat ccttcctttg tgtgctcgaa aattggagtc tgcccttctg
     1621 cctataagct gctgctggga accgagaagt gtgtctgggg cccaggctac tggtgtcaga
     1681 acatggagac tgctgcccga tgcaatgctg tcgatcattg caaacgccat gtgtggaact
     1741 agctttccag cttgcagaag tcgcctactt gtgggtctag ggtaatgaac acatagatct
     1801 atttgactta ataagtagga gccccctttt gtcttccccc atctcctgtc ccttactata
     1861 gcatttctgt cacgtaagag gcgctgacag ccacttccgt gtcccatttc tgctccaagg
     1921 atgaggacac ttgggcttca gctccccggc tgcccttttc acccacctgc tggagggggg
     1981 tggtgagcca gagggcagga gcatgtgctg agccctctcg gtgtgtaggg atcatggcca
     2041 tctcctagcg tgagggaact gcgcaacttc ctgtgctacc aaggagttta atttggatgc
     2101 ttagaagcac agagtgatca ggcctttaga gtgtgggatg gccattgcca cagcacagag
     2161 acttgagaag cacctgctgc aggtggcttg cttgggacgt tgccgtccct ggtcagcctt
     2221 cattctgctt tcccgtcttc ccgtctgcct tgttggggat ctgtggggta gggtggggag
     2281 ggggaacttc tgaatgtaac ttgcctgtgc cgtgtgacag tcgtgggcct gatcttttgt
     2341 ttgtgagacc cggaaatggg gtctctgcct ggctgtttgg ggtcccatac gcctttcgta
     2401 cccgtagctc tgttgagctg cttgttcacc tcatgggtgt aagaatcgtg gatcgttctg
     2461 gtgaggagac agccagcagg atgggcatgt gcagttaaat ggacctagat tttgttttgc
     2521 actaaagttt ctgtgactta ataaagttct gttaaccaga aaaaaaaaaa aaaaaa
//
