LOCUS       NM_138836               2208 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus serine protease 8 (Prss8), mRNA.
ACCESSION   NM_138836
VERSION     NM_138836.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Frateschi,S., Keppner,A., Malsure,S., Iwaszkiewicz,J., Sergi,C.,
            Merillat,A.M., Fowler-Jaeger,N., Randrianarison,N., Planes,C. and
            Hummler,E.
  TITLE     Mutations of the serine protease CAP1/Prss8 lead to reduced
            embryonic viability, skin defects, and decreased ENaC activity
  JOURNAL   Am J Pathol 181 (2), 605-615 (2012)
   PUBMED   22705055
  REMARK    GeneRIF: Mutations of the serine protease CAP1/Prss8 lead to
            reduced embryonic viability, skin defects, and decreased ENaC
            activity.
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Steensgaard,M., Svenningsen,P., Tinning,A.R., Nielsen,T.D.,
            Jorgensen,F., Kjaersgaard,G., Madsen,K. and Jensen,B.L.
  TITLE     Apical serine protease activity is necessary for assembly of a
            high-resistance renal collecting duct epithelium
  JOURNAL   Acta Physiol (Oxf) 200 (4), 347-359 (2010)
   PUBMED   20645929
  REMARK    GeneRIF: Data show that apical, GPI-anchored, lipid raft-associated
            serine protease activity, compatible with prostasin, is necessary
            for the development of a high-resistance collecting duct
            epithelium.
REFERENCE   3  (bases 1 to 2208)
  AUTHORS   Spacek,D.V., Perez,A.F., Ferranti,K.M., Wu,L.K., Moy,D.M.,
            Magnan,D.R. and King,T.R.
  TITLE     The mouse frizzy (fr) and rat 'hairless' (frCR) mutations are
            natural variants of protease serine S1 family member 8 (Prss8)
  JOURNAL   Exp Dermatol 19 (6), 527-532 (2010)
   PUBMED   20201958
  REMARK    GeneRIF: A 12-bp deletion in the third exon of the Prss8 gene is
            responsible for the mutant 'hairless', frizzy-Charles River (frCR)
            phenotype. No alterations to Prss8 coding sequences were detected
            in the allelic 'fuzzy' rat variant.
REFERENCE   4  (bases 1 to 2208)
  AUTHORS   Chen,M., Chen,L.M., Lin,C.Y. and Chai,K.X.
  TITLE     Hepsin activates prostasin and cleaves the extracellular domain of
            the epidermal growth factor receptor
  JOURNAL   Mol Cell Biochem 337 (1-2), 259-266 (2010)
   PUBMED   19911255
REFERENCE   5  (bases 1 to 2208)
  AUTHORS   Gonzalez-Begne,M., Lu,B., Han,X., Hagen,F.K., Hand,A.R.,
            Melvin,J.E. and Yates,J.R.
  TITLE     Proteomic analysis of human parotid gland exosomes by
            multidimensional protein identification technology (MudPIT)
  JOURNAL   J Proteome Res 8 (3), 1304-1314 (2009)
   PUBMED   19199708
REFERENCE   6  (bases 1 to 2208)
  AUTHORS   Narikiyo,T., Kitamura,K., Adachi,M., Miyoshi,T., Iwashita,K.,
            Shiraishi,N., Nonoguchi,H., Chen,L.M., Chai,K.X., Chao,J. and
            Tomita,K.
  TITLE     Regulation of prostasin by aldosterone in the kidney
  JOURNAL   J Clin Invest 109 (3), 401-408 (2002)
   PUBMED   11828000
REFERENCE   7  (bases 1 to 2208)
  AUTHORS   Chen,L.M. and Chai,K.X.
  TITLE     Prostasin serine protease inhibits breast cancer invasiveness and
            is transcriptionally regulated by promoter DNA methylation
  JOURNAL   Int J Cancer 97 (3), 323-329 (2002)
   PUBMED   11774283
REFERENCE   8  (bases 1 to 2208)
  AUTHORS   Mok,S.C., Chao,J., Skates,S., Wong,K., Yiu,G.K., Muto,M.G.,
            Berkowitz,R.S. and Cramer,D.W.
  TITLE     Prostasin, a potential serum marker for ovarian cancer:
            identification through microarray technology
  JOURNAL   J Natl Cancer Inst 93 (19), 1458-1464 (2001)
   PUBMED   11584061
REFERENCE   9  (bases 1 to 2208)
  AUTHORS   Adachi,M., Kitamura,K., Miyoshi,T., Narikiyo,T., Iwashita,K.,
            Shiraishi,N., Nonoguchi,H. and Tomita,K.
  TITLE     Activation of epithelial sodium channels by prostasin in Xenopus
            oocytes
  JOURNAL   J Am Soc Nephrol 12 (6), 1114-1121 (2001)
   PUBMED   11373334
REFERENCE   10 (bases 1 to 2208)
  AUTHORS   Laribi,A., Berteau,P., Gala,J., Eschwege,P., Benoit,G., Tombal,B.,
            Schmitt,F. and Loric,S.
  TITLE     Blood-borne RT-PCR assay for prostasin- specific transcripts to
            identify circulating prostate cells in cancer patients
  JOURNAL   Eur Urol 39 (1), 65-71 (2001)
   PUBMED   11173941
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AB017638.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB017638.1, BC061800.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132262,
                                           SAMD00132266 [ECO:0006172]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q37"
     gene            1..2208
                     /gene="Prss8"
                     /gene_synonym="CAP1"
                     /note="serine protease 8"
                     /db_xref="GeneID:192107"
                     /db_xref="RGD:619973"
     exon            1..308
                     /gene="Prss8"
                     /gene_synonym="CAP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             224..1252
                     /gene="Prss8"
                     /gene_synonym="CAP1"
                     /function="serine protease"
                     /note="prostasin; channel-activating protease 1; protease,
                     serine, 8"
                     /codon_start=1
                     /product="prostasin precursor"
                     /protein_id="NP_620191.1"
                     /db_xref="GeneID:192107"
                     /db_xref="RGD:619973"
                     /translation="MALRVGLGLGQLEALFILLLIGLLQSRIGADGTEASCGAVIQPR
                     ITGGGSAKPGQWPWQVSITYNGVHVCGGSLVSNQWVVSAAHCFPREHSKEEYEVKLGA
                     HQLDSFSNDIVVHTVAQIISHSSYREEGSQGDIALIRLSSPVTFSRYIRPICLPAANA
                     SFPNGLHCTVTGWGHVAPSVSLQTPRPLQQLEVPLISRETCSCLYNINAVPEEPHTIQ
                     QDMLCAGYVKGGKDACQGDSGGPLSCPIDGLWYLAGIVSWGDACGAPNRPGVYTLTST
                     YASWIHHHVAELQPRAVPQTQESQPDGHLCNHHPVFNLAAAQKLSRPILFLPLSLTLG
                     LFSLWLEH"
     exon            309..326
                     /gene="Prss8"
                     /gene_synonym="CAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            327..489
                     /gene="Prss8"
                     /gene_synonym="CAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            490..761
                     /gene="Prss8"
                     /gene_synonym="CAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            762..928
                     /gene="Prss8"
                     /gene_synonym="CAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            929..2208
                     /gene="Prss8"
                     /gene_synonym="CAP1"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2208
                     /gene="Prss8"
                     /gene_synonym="CAP1"
                     /note="14 a nucleotides"
ORIGIN      
        1 tgtgctgaac agggccacac tggtcactac agagaccaaa ggcgtgtccc gtcttctata
       61 ccctgtgtcc atttcctgcc gagaagcctg cttcaacctt cccagcctag atcctgggat
      121 tcttcacttc tgagcccaca cctagatcct gacttccaaa ccagccttca attccatccc
      181 tttctaccta gagtctcctt caggggggcc cttgtccggg gccatggccc taagggtggg
      241 cctgggactt gggcagctgg aagccctgtt tattctgctc cttattggat tgctccagtc
      301 aagaatcgga gctgatggga ctgaagcttc ctgtggtgca gtcatccagc cacgcatcac
      361 aggtggtggc agtgcgaagc ctggtcaatg gccctggcag gtcagcatca cctacaatgg
      421 cgtccacgtt tgtggtgggt ctctcgtgtc aaatcaatgg gtggtgtcag ctgctcactg
      481 cttccccaga gaacacagca aggaagagta tgaggtaaag ctgggggccc accagctgga
      541 ctccttcagc aatgacatcg tggtccacac agtagctcag atcatctccc actcgagcta
      601 ccgtgaggag ggctcccagg gtgacatcgc gctcatccgc ctcagcagcc ctgtcacctt
      661 ctcccgctac atcagaccca tctgcctccc tgcagccaat gcctcctttc ccaatggcct
      721 ccactgtact gtcacaggat ggggtcatgt ggctccttca gtgagcctcc agacccctag
      781 gcctctgcag cagcttgagg taccactcat cagccgtgaa acctgtagct gcctgtacaa
      841 cattaatgca gtgcctgaag aaccccacac tatccagcag gacatgctgt gcgctggcta
      901 tgttaaggga ggcaaggatg cttgccaggg tgactctgga ggcccactct cttgtcccat
      961 agacggcctc tggtatttgg caggcattgt gagttggggt gatgcgtgtg gcgcccccaa
     1021 ccggcctgga gtatatactc tgacttctac ctacgcttcc tggatccacc accacgtggc
     1081 agagctccag cctcgcgcgg ttccccagac tcaggagtcc cagccggacg gccacctctg
     1141 caaccatcat cctgtcttca acttagccgc agcccagaaa ttgtcaaggc ccatactttt
     1201 cctgccactg agtctgaccc tgggcctctt ctccctttgg cttgaacatt aagctactta
     1261 atcctcaaag ttgaccatgc ttccacgacc acagcacgcc caaggacatg tgcctgttca
     1321 ggaggtcagc acgccgactt gatctttgag cccactcttc tgcatttgct ccttgggatt
     1381 ataatttaat ctagggccaa gaagtttaca gcctgtggaa tggaccagga ctctagccac
     1441 ctttctctat tatctgcagt ttattggtta ggctcctatg gagctcccag aagcctcact
     1501 tcaacctgta tttagcacac agcagctgcc tagctgactg ctgcagaagg ctggcttcag
     1561 ggccgaccca ccctgcctga tgagcccttc acttcgtcct cttcctgcct ccagggcttt
     1621 gcctgtcttt ctgacaggga agacagcagc cttaatctcc tggactccgg aggactgagc
     1681 ccccaccgga gctggctggg gctggggtca gggtgaggag taaaaggagg aaggaggaaa
     1741 tgtttttaac acaactggct ggatgtgaaa tgaaatgaag aatatcaccc ttcagcactt
     1801 cttcctgcct ccgaagtcca gcacaccagc agaatttgct ttattaataa atgacacaaa
     1861 acaggtctgt acacatattt acaggttaga ggccaggaaa cacagatcca aagaagggca
     1921 caggccattc acttcttgga gagcttgact tgtttgtgct tcgggggtgc ccacttgagg
     1981 cagacagagt ccactgtggg aggaaagagg aatgagaggt cagcaggacc tctgggccaa
     2041 gtctgcttac acctggctgc ccctctgggc ccaggccagg tttcaccttc acgggatgag
     2101 aacaaaaggg agctttggac ctggggggag gggatcccag gaatgagtaa ccacctctag
     2161 gctgctgtcc tagaaactgg ttcaactggt ttggatcttg aggagccc
//
