LOCUS       NP_620191                342 aa            linear   ROD 24-JUN-2024
DEFINITION  prostasin precursor [Rattus norvegicus].
ACCESSION   NP_620191
VERSION     NP_620191.1
DBSOURCE    REFSEQ: accession NM_138836.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 342)
  AUTHORS   Frateschi,S., Keppner,A., Malsure,S., Iwaszkiewicz,J., Sergi,C.,
            Merillat,A.M., Fowler-Jaeger,N., Randrianarison,N., Planes,C. and
            Hummler,E.
  TITLE     Mutations of the serine protease CAP1/Prss8 lead to reduced
            embryonic viability, skin defects, and decreased ENaC activity
  JOURNAL   Am J Pathol 181 (2), 605-615 (2012)
   PUBMED   22705055
  REMARK    GeneRIF: Mutations of the serine protease CAP1/Prss8 lead to
            reduced embryonic viability, skin defects, and decreased ENaC
            activity.
REFERENCE   2  (residues 1 to 342)
  AUTHORS   Steensgaard,M., Svenningsen,P., Tinning,A.R., Nielsen,T.D.,
            Jorgensen,F., Kjaersgaard,G., Madsen,K. and Jensen,B.L.
  TITLE     Apical serine protease activity is necessary for assembly of a
            high-resistance renal collecting duct epithelium
  JOURNAL   Acta Physiol (Oxf) 200 (4), 347-359 (2010)
   PUBMED   20645929
  REMARK    GeneRIF: Data show that apical, GPI-anchored, lipid raft-associated
            serine protease activity, compatible with prostasin, is necessary
            for the development of a high-resistance collecting duct
            epithelium.
REFERENCE   3  (residues 1 to 342)
  AUTHORS   Spacek,D.V., Perez,A.F., Ferranti,K.M., Wu,L.K., Moy,D.M.,
            Magnan,D.R. and King,T.R.
  TITLE     The mouse frizzy (fr) and rat 'hairless' (frCR) mutations are
            natural variants of protease serine S1 family member 8 (Prss8)
  JOURNAL   Exp Dermatol 19 (6), 527-532 (2010)
   PUBMED   20201958
  REMARK    GeneRIF: A 12-bp deletion in the third exon of the Prss8 gene is
            responsible for the mutant 'hairless', frizzy-Charles River (frCR)
            phenotype. No alterations to Prss8 coding sequences were detected
            in the allelic 'fuzzy' rat variant.
REFERENCE   4  (residues 1 to 342)
  AUTHORS   Chen,M., Chen,L.M., Lin,C.Y. and Chai,K.X.
  TITLE     Hepsin activates prostasin and cleaves the extracellular domain of
            the epidermal growth factor receptor
  JOURNAL   Mol Cell Biochem 337 (1-2), 259-266 (2010)
   PUBMED   19911255
REFERENCE   5  (residues 1 to 342)
  AUTHORS   Gonzalez-Begne,M., Lu,B., Han,X., Hagen,F.K., Hand,A.R.,
            Melvin,J.E. and Yates,J.R.
  TITLE     Proteomic analysis of human parotid gland exosomes by
            multidimensional protein identification technology (MudPIT)
  JOURNAL   J Proteome Res 8 (3), 1304-1314 (2009)
   PUBMED   19199708
REFERENCE   6  (residues 1 to 342)
  AUTHORS   Narikiyo,T., Kitamura,K., Adachi,M., Miyoshi,T., Iwashita,K.,
            Shiraishi,N., Nonoguchi,H., Chen,L.M., Chai,K.X., Chao,J. and
            Tomita,K.
  TITLE     Regulation of prostasin by aldosterone in the kidney
  JOURNAL   J Clin Invest 109 (3), 401-408 (2002)
   PUBMED   11828000
REFERENCE   7  (residues 1 to 342)
  AUTHORS   Chen,L.M. and Chai,K.X.
  TITLE     Prostasin serine protease inhibits breast cancer invasiveness and
            is transcriptionally regulated by promoter DNA methylation
  JOURNAL   Int J Cancer 97 (3), 323-329 (2002)
   PUBMED   11774283
REFERENCE   8  (residues 1 to 342)
  AUTHORS   Mok,S.C., Chao,J., Skates,S., Wong,K., Yiu,G.K., Muto,M.G.,
            Berkowitz,R.S. and Cramer,D.W.
  TITLE     Prostasin, a potential serum marker for ovarian cancer:
            identification through microarray technology
  JOURNAL   J Natl Cancer Inst 93 (19), 1458-1464 (2001)
   PUBMED   11584061
REFERENCE   9  (residues 1 to 342)
  AUTHORS   Adachi,M., Kitamura,K., Miyoshi,T., Narikiyo,T., Iwashita,K.,
            Shiraishi,N., Nonoguchi,H. and Tomita,K.
  TITLE     Activation of epithelial sodium channels by prostasin in Xenopus
            oocytes
  JOURNAL   J Am Soc Nephrol 12 (6), 1114-1121 (2001)
   PUBMED   11373334
REFERENCE   10 (residues 1 to 342)
  AUTHORS   Laribi,A., Berteau,P., Gala,J., Eschwege,P., Benoit,G., Tombal,B.,
            Schmitt,F. and Loric,S.
  TITLE     Blood-borne RT-PCR assay for prostasin- specific transcripts to
            identify circulating prostate cells in cancer patients
  JOURNAL   Eur Urol 39 (1), 65-71 (2001)
   PUBMED   11173941
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AB017638.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB017638.1, BC061800.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132262,
                                           SAMD00132266 [ECO:0006172]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Rattus norvegicus"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q37"
     Protein         1..342
                     /product="prostasin precursor"
                     /function="serine protease"
                     /note="prostasin; channel-activating protease 1; protease,
                     serine, 8"
                     /calculated_mol_wt=33782
     sig_peptide     1..29
                     /note="/evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9ES87.3)"
                     /calculated_mol_wt=3079
     mat_peptide     33..322
                     /product="Prostasin. /id=PRO_0000240513"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9ES87.3)"
                     /calculated_mol_wt=31247
     Region          45..284
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cd00190"
                     /db_xref="CDD:238113"
     Site            45
                     /site_type="active"
                     /note="cleavage site [active]"
                     /db_xref="CDD:238113"
     Site            order(85,134,238)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            159
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9ES87.3)"
     Site            order(232,257,259)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     Site            320..340
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9ES87.3)"
     CDS             1..342
                     /gene="Prss8"
                     /gene_synonym="CAP1"
                     /coded_by="NM_138836.1:224..1252"
                     /db_xref="GeneID:192107"
                     /db_xref="RGD:619973"
ORIGIN      
        1 malrvglglg qlealfilll igllqsriga dgteascgav iqpritgggs akpgqwpwqv
       61 sityngvhvc ggslvsnqwv vsaahcfpre hskeeyevkl gahqldsfsn divvhtvaqi
      121 ishssyreeg sqgdialirl sspvtfsryi rpiclpaana sfpnglhctv tgwghvapsv
      181 slqtprplqq levplisret csclyninav peephtiqqd mlcagyvkgg kdacqgdsgg
      241 plscpidglw ylagivswgd acgapnrpgv ytltstyasw ihhhvaelqp ravpqtqesq
      301 pdghlcnhhp vfnlaaaqkl srpilflpls ltlglfslwl eh
//
