LOCUS       NM_053504               2499 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Rattus norvegicus serine protease 12 (Prss12), mRNA.
ACCESSION   NM_053504
VERSION     NM_053504.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ozhogina,O.A., Grishaev,A., Bominaar,E.L., Patthy,L., Trexler,M.
            and Llinas,M.
  TITLE     NMR solution structure of the neurotrypsin Kringle domain
  JOURNAL   Biochemistry 47 (47), 12290-12298 (2008)
   PUBMED   18956887
  REMARK    GeneRIF: NMR structure of neurotrypsin kringle domain
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Stephan,A., Mateos,J.M., Kozlov,S.V., Cinelli,P., Kistler,A.D.,
            Hettwer,S., Rulicke,T., Streit,P., Kunz,B. and Sonderegger,P.
  TITLE     Neurotrypsin cleaves agrin locally at the synapse
  JOURNAL   FASEB J 22 (6), 1861-1873 (2008)
   PUBMED   18230682
REFERENCE   3  (bases 1 to 2499)
  AUTHORS   Frischknecht,R., Fejtova,A., Viesti,M., Stephan,A. and
            Sonderegger,P.
  TITLE     Activity-induced synaptic capture and exocytosis of the neuronal
            serine protease neurotrypsin
  JOURNAL   J Neurosci 28 (7), 1568-1579 (2008)
   PUBMED   18272678
REFERENCE   4  (bases 1 to 2499)
  AUTHORS   Reif,R., Sales,S., Hettwer,S., Dreier,B., Gisler,C., Wolfel,J.,
            Luscher,D., Zurlinden,A., Stephan,A., Ahmed,S., Baici,A.,
            Ledermann,B., Kunz,B. and Sonderegger,P.
  TITLE     Specific cleavage of agrin by neurotrypsin, a synaptic protease
            linked to mental retardation
  JOURNAL   FASEB J 21 (13), 3468-3478 (2007)
   PUBMED   17586728
REFERENCE   5  (bases 1 to 2499)
  AUTHORS   Mitsui,S., Yamaguchi,N., Osako,Y. and Yuri,K.
  TITLE     Enzymatic properties and localization of motopsin (PRSS12), a
            protease whose absence causes mental retardation
  JOURNAL   Brain Res 1136 (1), 1-12 (2007)
   PUBMED   17223089
REFERENCE   6  (bases 1 to 2499)
  AUTHORS   Molinari,F., Rio,M., Meskenaite,V., Encha-Razavi,F., Auge,J.,
            Bacq,D., Briault,S., Vekemans,M., Munnich,A., Attie-Bitach,T.,
            Sonderegger,P. and Colleaux,L.
  TITLE     Truncating neurotrypsin mutation in autosomal recessive
            nonsyndromic mental retardation
  JOURNAL   Science 298 (5599), 1779-1781 (2002)
   PUBMED   12459588
REFERENCE   7  (bases 1 to 2499)
  AUTHORS   Gschwend,T.P., Krueger,S.R., Kozlov,S.V., Wolfer,D.P. and
            Sonderegger,P.
  TITLE     Neurotrypsin, a novel multidomain serine protease expressed in the
            nervous system
  JOURNAL   Mol Cell Neurosci 9 (3), 207-219 (1997)
   PUBMED   9245503
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AJ311671.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ311671.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760393, SAMEA5760396
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q42"
     gene            1..2499
                     /gene="Prss12"
                     /gene_synonym="Nt"
                     /note="serine protease 12"
                     /db_xref="GeneID:85266"
                     /db_xref="RGD:69238"
     exon            1..660
                     /gene="Prss12"
                     /gene_synonym="Nt"
                     /inference="alignment:Splign:2.1.0"
     CDS             183..2468
                     /gene="Prss12"
                     /gene_synonym="Nt"
                     /note="neurotrypsin; peptidase, serine, 12 (neurotrypsin,
                     motopsin); protease, serine, 12 neurotrypsin (motopsin);
                     protease, serine 12"
                     /codon_start=1
                     /product="neurotrypsin precursor"
                     /protein_id="NP_445956.1"
                     /db_xref="GeneID:85266"
                     /db_xref="RGD:69238"
                     /translation="MALARCVLAVILGVLSEVARADPVLHSPLHRPHPSPPRSQHAHY
                     LPSSRRPPRTPRFPLPPRAPAAQRPQLLSTRHTPPTISRRCGAGEPWGNATNLGVPCL
                     HWDEVPPFLERSPPASWAELRGQPHNFCRSPDGAGRPWCFYRNAQGKVDWGYCDCGQG
                     PALPVIRLVGGKSGHEGRVELYHAGQWGTICDDQWDDADAEVICRQLGLSGIAKAWHQ
                     AHFGEGSGPILLDEVRCTGNELSIEQCPKSSWGEHNCGHKEDAGVSCAPLTDGVIRLS
                     GGKSVHEGRLEVYYRGQWGTVCDDGWTEMNTYVACRLLGFKYGKQSSVNHFEGSSRPI
                     WLDDVSCSGKEASFIQCSRRQWGRHDCSHREDVGLTCYPDSDGHRLSPGFPIRLMDGE
                     NKREGRVEVFVNGQWGTICDDGWTDKHAAVICRQLGYKGPARARTMAYFGEGKGPIHM
                     DNVKCTGNEKALADCVKQDIGRHNCRHSEDAGVICDYYEKKTSGHGNKETLSSGCGLR
                     LLHRRQTRIIGGNNSLRGAWPWQASLRLKSTHGDGRLLCGATLLSSCWVLTAAHCFTR
                     YGNNSRSYAVRVGDYHTLVPEGFEQDIGVQQIVIHRNYRPDSSDYDIALVRLQGSGEQ
                     CARLSTHVLPACLPLWRERPQKTASNCHITGWGDTGRAYSRTLQQAAVPLLPKRFCKE
                     RYKGLFTGRMLCAGNLQEDNRVDSCQGDSGGPLMCEKPDETWVVYGVTSWGYGCGIKD
                     TPGVYTRVPAFVPWIKSVTSL"
     exon            661..811
                     /gene="Prss12"
                     /gene_synonym="Nt"
                     /inference="alignment:Splign:2.1.0"
     exon            812..990
                     /gene="Prss12"
                     /gene_synonym="Nt"
                     /inference="alignment:Splign:2.1.0"
     exon            991..1132
                     /gene="Prss12"
                     /gene_synonym="Nt"
                     /inference="alignment:Splign:2.1.0"
     exon            1133..1329
                     /gene="Prss12"
                     /gene_synonym="Nt"
                     /inference="alignment:Splign:2.1.0"
     exon            1330..1471
                     /gene="Prss12"
                     /gene_synonym="Nt"
                     /inference="alignment:Splign:2.1.0"
     exon            1472..1677
                     /gene="Prss12"
                     /gene_synonym="Nt"
                     /inference="alignment:Splign:2.1.0"
     exon            1678..1756
                     /gene="Prss12"
                     /gene_synonym="Nt"
                     /inference="alignment:Splign:2.1.0"
     exon            1757..1879
                     /gene="Prss12"
                     /gene_synonym="Nt"
                     /inference="alignment:Splign:2.1.0"
     exon            1880..2160
                     /gene="Prss12"
                     /gene_synonym="Nt"
                     /inference="alignment:Splign:2.1.0"
     exon            2161..2499
                     /gene="Prss12"
                     /gene_synonym="Nt"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccagcctttg cttcgagggg ctgacccttc gggtcccggt gtgattctcc agccgcccta
       61 ggggcccgga cagcagggcg gcggcgcgag cgtgggaggg ggctctagga ctctgcccgc
      121 cccgccccgc cccctccgcg gggacccgga gcccagcatg gaccacactc ggcgccgcag
      181 ccatggcgct cgcccgctgc gtgctggctg tgattttagg ggtactgtcc gaagtggccc
      241 gcgctgatcc ggtcttgcac tcgcccctgc accgcccgca tccgtctcca ccgcgttccc
      301 aacacgcgca ttaccttccc agctcgcggc ggccacccag gaccccgcgc ttcccgctcc
      361 cgccgcgggc ccccgctgcc cagcgccctc agctcctcag cacccggcac acgcccccga
      421 cgatttcacg ccggtgcggg gctggagagc cctggggcaa tgccaccaac ctcggcgtcc
      481 cgtgtctaca ctgggacgag gtgccgccct tcctggagcg gtcgcccccg gccagttggg
      541 ctgagctaag agggcagccg cacaacttct gccggagtcc ggatggcgcg ggcagacctt
      601 ggtgcttcta tcgaaatgcc caaggcaaag tggactgggg ctactgcgat tgtggtcaag
      661 gccccgcgct gcctgtcatt cgccttgttg gtgggaagag tgggcacgaa ggtcgagtgg
      721 agctgtacca cgctggccaa tggggaacca tctgtgatga ccaatgggac gacgcagatg
      781 cagaagtcat ctgcaggcag ctgggtctca gtggcattgc caaagcatgg catcaggcac
      841 attttgggga aggatctggc ccaatattgt tggatgaagt acgctgcact gggaatgagc
      901 tgtcaattga gcagtgtcca aagagctcct ggggtgaaca taactgtggc cataaagaag
      961 atgccggagt gtcttgtgct cctctgacag atggtgtcat cagactgtca ggagggaaaa
     1021 gtgtccatga aggtcgcttg gaggtgtact ataggggaca gtggggaaca gtttgtgatg
     1081 atggctggac tgaaatgaat acatacgtgg cttgtcgact gctgggattt aaatatggca
     1141 aacagtcctc tgtgaaccac tttgaaggca gcagcaggcc catatggctg gatgacgtca
     1201 gctgctcagg aaaagaagcc agcttcattc agtgttccag gagacagtgg ggaaggcatg
     1261 actgcagcca tagggaagat gtgggcctca cctgctaccc tgacagtgat ggtcataggc
     1321 tttccccagg ttttcctatc agactaatgg acggagagaa taagagagaa ggacgagtgg
     1381 aggtttttgt caatggccaa tggggaacga tctgcgatga tggatggacc gacaagcatg
     1441 cagctgtgat ttgccggcag cttggctata agggtccggc cagggcgagg accatggctt
     1501 attttgggga aggaaaaggc cccatccaca tggacaatgt gaagtgcaca gggaatgaga
     1561 aggccctggc cgactgcgtc aaacaagaca tcggaaggca caactgccgc cacagtgagg
     1621 acgcaggagt catctgtgac tattacgaga aaaaaacatc aggtcatggg aataaagaga
     1681 ccctctcatc tgggtgtgga ttgagattac tacaccgccg acagacacgg atcattggtg
     1741 ggaacaattc tttaaggggt gcctggcctt ggcaggcttc cctcaggctg aagtcgaccc
     1801 acggagatgg caggctgctc tgtggagcca cccttctaag tagctgctgg gtcctgacag
     1861 ctgcgcactg tttcacaagg tacggtaaca actcgaggag ttatgccgtc cgagttggcg
     1921 attatcatac tttggttccc gaggggtttg aacaagacat aggggtccaa cagattgtga
     1981 ttcacaggaa ctatcggcca gacagcagcg actatgacat cgccctggtc agattacaag
     2041 gctcagggga gcaatgtgcc agactaagca cccacgtttt gccagcctgt ttacctctat
     2101 ggagagaaag gccacagaaa acagcctcca actgtcacat aacaggatgg ggagacacag
     2161 gtcgtgctta ctcaagaact ctacaacaag cagctgtgcc tttgttaccc aagaggtttt
     2221 gtaaggaacg ttacaaggga ctgtttactg ggagaatgct ctgtgctgga aacctccaag
     2281 aagacaaccg tgtggacagc tgccagggag acagcggagg accacttatg tgtgaaaagc
     2341 ccgatgaaac ttgggttgtg tatggggtga cttcctgggg gtacggatgc ggaatcaaag
     2401 acactccagg agtttatacc agagtccctg cctttgtacc ttggataaaa agtgttacga
     2461 gtctgtaatt tatgggaagc tcaagaaaaa gagtaaagg
//
