LOCUS       NM_001191590            7246 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus pre-mRNA processing factor 8 (Prpf8), mRNA.
ACCESSION   NM_001191590 XM_213385
VERSION     NM_001191590.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Zhan,X., Yan,C., Zhang,X., Lei,J. and Shi,Y.
  TITLE     Structures of the human pre-catalytic spliceosome and its precursor
            spliceosome
  JOURNAL   Cell Res 28 (12), 1129-1140 (2018)
   PUBMED   30315277
REFERENCE   2  (bases 1 to 7246)
  AUTHORS   Zhan,X., Yan,C., Zhang,X., Lei,J. and Shi,Y.
  TITLE     Structure of a human catalytic step I spliceosome
  JOURNAL   Science 359 (6375), 537-545 (2018)
   PUBMED   29301961
REFERENCE   3  (bases 1 to 7246)
  AUTHORS   Haselbach,D., Komarov,I., Agafonov,D.E., Hartmuth,K., Graf,B.,
            Dybkov,O., Urlaub,H., Kastner,B., Luhrmann,R. and Stark,H.
  TITLE     Structure and Conformational Dynamics of the Human Spliceosomal
            Bact Complex
  JOURNAL   Cell 172 (3), 454-464 (2018)
   PUBMED   29361316
REFERENCE   4  (bases 1 to 7246)
  AUTHORS   Bertram,K., Agafonov,D.E., Dybkov,O., Haselbach,D., Leelaram,M.N.,
            Will,C.L., Urlaub,H., Kastner,B., Luhrmann,R. and Stark,H.
  TITLE     Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for
            Activation
  JOURNAL   Cell 170 (4), 701-713 (2017)
   PUBMED   28781166
REFERENCE   5  (bases 1 to 7246)
  AUTHORS   Bertram,K., Agafonov,D.E., Liu,W.T., Dybkov,O., Will,C.L.,
            Hartmuth,K., Urlaub,H., Kastner,B., Stark,H. and Luhrmann,R.
  TITLE     Cryo-EM structure of a human spliceosome activated for step 2 of
            splicing
  JOURNAL   Nature 542 (7641), 318-323 (2017)
   PUBMED   28076346
REFERENCE   6  (bases 1 to 7246)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   7  (bases 1 to 7246)
  AUTHORS   Jurica,M.S., Licklider,L.J., Gygi,S.R., Grigorieff,N. and
            Moore,M.J.
  TITLE     Purification and characterization of native spliceosomes suitable
            for three-dimensional structural analysis
  JOURNAL   RNA 8 (4), 426-439 (2002)
   PUBMED   11991638
REFERENCE   8  (bases 1 to 7246)
  AUTHORS   McKie,A.B., McHale,J.C., Keen,T.J., Tarttelin,E.E., Goliath,R., van
            Lith-Verhoeven,J.J., Greenberg,J., Ramesar,R.S., Hoyng,C.B.,
            Cremers,F.P., Mackey,D.A., Bhattacharya,S.S., Bird,A.C.,
            Markham,A.F. and Inglehearn,C.F.
  TITLE     Mutations in the pre-mRNA splicing factor gene PRPC8 in autosomal
            dominant retinitis pigmentosa (RP13)
  JOURNAL   Hum Mol Genet 10 (15), 1555-1562 (2001)
   PUBMED   11468273
REFERENCE   9  (bases 1 to 7246)
  AUTHORS   Haas,D.W., Shepherd,V.L. and Hagedorn,C.H.
  TITLE     Lipopolysaccharide stimulates phosphorylation of eukaryotic
            initiation factor-4F in macrophages and tumor necrosis factor
            participates in this event
  JOURNAL   Second Messengers Phosphoproteins 14 (3), 163-171 (1992)
   PUBMED   1345341
REFERENCE   10 (bases 1 to 7246)
  AUTHORS   Guialis,A., Moraitou,M., Patrinou-Georgoula,M. and Dangli,A.
  TITLE     A novel 40S multi-snRNP complex isolated from rat liver nuclei
  JOURNAL   Nucleic Acids Res 19 (2), 287-296 (1991)
   PUBMED   1707521
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000010.1.
            
            On Jul 17, 2010 this sequence version replaced XM_213385.5.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643292.41752.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760400, SAMN12840111
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-70                JAXUCZ010000010.1  60829778-60829847
            71-181              JAXUCZ010000010.1  60830039-60830149
            182-350             JAXUCZ010000010.1  60830628-60830796
            351-515             JAXUCZ010000010.1  60831991-60832155
            516-734             JAXUCZ010000010.1  60832260-60832478
            735-947             JAXUCZ010000010.1  60832591-60832803
            948-1073            JAXUCZ010000010.1  60832918-60833043
            1074-1179           JAXUCZ010000010.1  60833123-60833228
            1180-1370           JAXUCZ010000010.1  60833781-60833971
            1371-1490           JAXUCZ010000010.1  60834320-60834439
            1491-1680           JAXUCZ010000010.1  60834524-60834713
            1681-1800           JAXUCZ010000010.1  60834822-60834941
            1801-1935           JAXUCZ010000010.1  60835050-60835184
            1936-2065           JAXUCZ010000010.1  60835513-60835642
            2066-2262           JAXUCZ010000010.1  60835895-60836091
            2263-2469           JAXUCZ010000010.1  60836353-60836559
            2470-2633           JAXUCZ010000010.1  60836678-60836841
            2634-2760           JAXUCZ010000010.1  60836984-60837110
            2761-2953           JAXUCZ010000010.1  60837221-60837413
            2954-3141           JAXUCZ010000010.1  60837893-60838080
            3142-3380           JAXUCZ010000010.1  60838255-60838493
            3381-3527           JAXUCZ010000010.1  60838695-60838841
            3528-3738           JAXUCZ010000010.1  60839021-60839231
            3739-3855           JAXUCZ010000010.1  60839832-60839948
            3856-4103           JAXUCZ010000010.1  60843903-60844150
            4104-4283           JAXUCZ010000010.1  60844259-60844438
            4284-4419           JAXUCZ010000010.1  60844622-60844757
            4420-4589           JAXUCZ010000010.1  60844872-60845041
            4590-4719           JAXUCZ010000010.1  60845198-60845327
            4720-4866           JAXUCZ010000010.1  60845427-60845573
            4867-5027           JAXUCZ010000010.1  60845804-60845964
            5028-5219           JAXUCZ010000010.1  60846618-60846809
            5220-5457           JAXUCZ010000010.1  60847207-60847444
            5458-5586           JAXUCZ010000010.1  60847575-60847703
            5587-5700           JAXUCZ010000010.1  60848996-60849109
            5701-5874           JAXUCZ010000010.1  60849183-60849356
            5875-6068           JAXUCZ010000010.1  60849601-60849794
            6069-6308           JAXUCZ010000010.1  60850858-60851097
            6309-6450           JAXUCZ010000010.1  60851196-60851337
            6451-6591           JAXUCZ010000010.1  60851709-60851849
            6592-6731           JAXUCZ010000010.1  60851960-60852099
            6732-6934           JAXUCZ010000010.1  60852209-60852411
            6935-7246           JAXUCZ010000010.1  60852576-60852887
FEATURES             Location/Qualifiers
     source          1..7246
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q24"
     gene            1..7246
                     /gene="Prpf8"
                     /note="pre-mRNA processing factor 8"
                     /db_xref="GeneID:287530"
                     /db_xref="RGD:1305467"
     exon            1..70
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            71..181
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..7089
                     /gene="Prpf8"
                     /note="PRP8 pre-mRNA processing factor 8 homolog"
                     /codon_start=1
                     /product="pre-mRNA-processing-splicing factor 8"
                     /protein_id="NP_001178519.1"
                     /db_xref="GeneID:287530"
                     /db_xref="RGD:1305467"
                     /translation="MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKR
                     YAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKL
                     LENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRH
                     FKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRK
                     YVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGG
                     PKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHT
                     PNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTP
                     LYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKL
                     LKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLI
                     HRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRL
                     GNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKG
                     PGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFD
                     LELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMIL
                     RYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDG
                     PYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRL
                     NQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPL
                     EKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGE
                     CNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGI
                     IRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLF
                     CRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHD
                     VNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSY
                     EEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNK
                     WNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPK
                     ELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESE
                     FIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGW
                     RVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTY
                     FPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQ
                     VQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQ
                     KETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRW
                     GDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLI
                     QQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTN
                     VYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTA
                     EEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVE
                     KFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDK
                     TTVTEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEI
                     SAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWR
                     VRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVS
                     PPDNPQVKEIRCIVMVPQWGTHQTVHLPSQLPQHEYLKEMEPLGWIHTQPNESPQLSP
                     QDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNP
                     KGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYH
                     EVHRPSHFLNFALLQEGEVYSADREDLYA"
     exon            182..350
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            351..515
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            516..734
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            735..947
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            948..1073
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            1074..1179
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            1180..1370
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            1371..1490
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            1491..1680
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            1681..1800
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            1801..1935
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            1936..2065
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            2066..2262
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            2263..2469
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            2470..2633
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            2634..2760
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            2761..2953
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            2954..3141
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            3142..3380
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            3381..3527
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            3528..3738
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            3739..3855
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            3856..4103
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            4104..4283
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            4284..4419
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            4420..4589
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            4590..4719
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            4720..4866
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            4867..5027
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            5028..5219
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            5220..5457
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            5458..5586
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            5587..5700
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            5701..5874
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            5875..6068
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            6069..6308
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            6309..6450
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            6451..6591
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            6592..6731
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            6732..6934
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
     exon            6935..7246
                     /gene="Prpf8"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 acgcggagcg gacgtcgtct ttccgccatt gctctgcgag ccctgccgct acctaagtga
       61 gggacgacag attctcccgg catggccgga gtgtttcctt acagagggcc gggtaacccg
      121 gtgcccggcc ctctagcccc gctgccggat tacatgtcag aggagaagct gcaggagaaa
      181 gctcgaaaat ggcagcagtt gcaggccaag cgctatgcag agaagcggaa attcgggttt
      241 gtggatgctc agaaggaaga catgccccca gaacatgtca ggaaaatcat tcgagaccat
      301 ggagacatga ccaacaggaa gttccgtcat gacaaaaggg tctatttggg agctctcaag
      361 tacatgcctc acgcagttct caagctccta gagaacatgc ccatgccttg ggagcagatc
      421 cgggatgtgc ctgtgctgta ccacatcact ggagccattt ccttcgttaa cgagattccc
      481 tgggttattg agccagttta catctcccag tgggggtcaa tgtggatcat gatgcgtaga
      541 gaaaaaagag acaggaggca tttcaagagg atgcgttttc ccccttttga tgatgaggag
      601 ccgcccctgg attatgctga caacatctta gatgtcgagc cactggaagc cattcagcta
      661 gagctggacc ctgaggagga tgcccctgtg ttggactggt tctatgacca ccagccattg
      721 agagatagta ggaagtatgt taatggttcc acctaccaac gctggcagtt cacattgcct
      781 atgatgtcca ctctctaccg actggccaac cagcttctga ctgacttggt agatgacaac
      841 tacttttatc tctttgatct aaaggccttc tttacctcta aggcactcaa catggccatt
      901 cctgggggac ccaaatttga acctcttgtt cgagacatca acctacagga tgaagactgg
      961 aatgaattca atgatattaa caagatcatc atccgtcagc ctattcgtac tgagtacaag
     1021 attgcttttc cgtacctcta taacaacctc ccacaccatg tccacctgac ctggtaccac
     1081 actcctaacg ttgtgttcat caagactgag gatcctgatc tgccagcttt ctactttgat
     1141 cctctgatta acccaatctc ccatagacac tcagtcaaga gccaagagcc actacctgat
     1201 gatgatgagg agtttgagct cccagagttt gtggagccct tcctgaagga tacacctctc
     1261 tatacagaca atacagccaa tggcatcgca ctgctctggg caccacggcc cttcaaccta
     1321 cgctctggtc gtacccgccg ggccctcgac attccccttg tcaagaactg gtatcgggag
     1381 cactgtcctg ctgggcaacc tgtgaaagtt cgagtctcct atcagaagct gcttaagtat
     1441 tatgtgctga atgccctcaa acatcggcct cccaaagcgc agaagaagag gtatctgttt
     1501 cgctctttca aagctaccaa attctttcag tctaccaagc tggactgggt ggaggtcggg
     1561 ctgcaggttt gccgccaggg ctacaacatg cttaaccttc tcattcaccg taagaacctc
     1621 aactatctgc atttggacta taacttcaac ctgaagcctg tcaagacgct caccaccaag
     1681 gaaagaaaaa aatctcgctt tgggaatgct ttccatctgt gtcgggaagt actgcgtttg
     1741 actaagctgg ttgtggatag tcatgtgcag tatcgcttgg gcaatgtaga tgccttccag
     1801 ctggcagatg gattgcagta tatatttgcc cacgtgggac agttgacagg catgtatcga
     1861 tacaaatata agctgatgcg gcagatccgc atgtgcaagg acttgaaaca tctcatctat
     1921 taccgcttca acacgggtcc tgtggggaaa ggccctggct gtggtttctg ggctgctggc
     1981 tggcgggtct ggctattttt tatgcgtgga attacccctt tgctagaacg atggctaggc
     2041 aaccttctgg cccgacagtt tgaaggccgt cactcaaagg gggtggcaaa gacagtaaca
     2101 aagcaacgag tagagtccca ttttgacctt gagcttaggg cagctgtgat gcatgatatt
     2161 ctggacatga tgcctgaagg catcaaacag aacaaggccc gaacaattct gcagcacctc
     2221 agtgaggcct ggcgctgctg gaaggccaac attccatgga aggtcccagg actgccaacg
     2281 cctatagaga atatgattct tcggtacgtt aaggccaagg ctgattggtg gaccaatact
     2341 gcccactaca accgtgaacg gattcgccgt ggggctactg tagacaagac tgtttgcaaa
     2401 aagaacctgg gccgcctcac ccgcctgtat ctgaaggcag aacaggagcg gcagcacaat
     2461 tacctgaagg acgggcctta catcacagca gaggaagcag tggcagtgta tactaccact
     2521 gtgcactggc tggaaagccg taggttctct cctatcccgt tccccccact ctcctacaag
     2581 catgacacca agttgcttat cttggctctg gagcgactca aggaagctta tagtgtgaag
     2641 tctaggttaa accagtctca gagggaggag ctgggtctga ttgaacaagc ctatgacaac
     2701 ccccatgagg ctttgtcccg catcaagcgt cacctcctca cgcagagagc ctttaaggaa
     2761 gtgggcattg agtttatgga tctctatagc caccttgtgc cagtgtatga tgtggaacca
     2821 ctggagaaaa taactgatgc ttacctggac cagtacctgt ggtatgaagc agataaacgc
     2881 cgtcttttcc cgccctggat caagcctgct gacacagaac cccctccact gcttgtttac
     2941 aagtggtgcc aaggcatcaa taacctgcag gatgtgtggg agacgagtga gggtgagtgc
     3001 aatgtcatgc tggagtctcg gtttgagaag atgtatgaga agattgacct gaccctgctc
     3061 aacaggctgc tgcgactcat tgtggaccac aacatagctg actacatgac agccaagaac
     3121 aatgtagtca tcaactataa ggacatgaac catacaaatt catatggaat catcagaggc
     3181 ctgcagtttg cctcattcat tgtacagtac tatggtttgg tgatggattt gcttgtattg
     3241 ggattgcacc gggccagtga gatggctggg cctccgcaga tgccaaatga ctttctcagt
     3301 tttcaggaca tagccaccga ggctgcacac ccaatccgac tcttctgtag atacattgat
     3361 cgcatccata ttttcttcag gttcacagca gatgaggctc gggatctgat ccagcgttac
     3421 ctgacagaac atccagaccc caataatgaa aacattgttg gctacaataa taagaaatgc
     3481 tggccccgag atgcccgtat gcgtctcatg aagcatgatg tcaacttggg tcgggcagtg
     3541 ttctgggaca tcaaaaaccg gctgccacga tcggtgacta cagtacagtg ggagaatagc
     3601 tttgtgtctg tgtacagtaa agacaacccc aacctgctgt tcaacatgtg tggctttgag
     3661 tgccgcatcc tacctaagtg ccgtaccagc tatgaagagt tcacccacaa ggacggggtc
     3721 tggaatctac agaatgaggt tactaaggag cggacggctc agtgtttcct gcgtgtggac
     3781 gatgagtcaa tgcagcgctt ccataaccgc gtgcggcaga ttctgatggc ctctggctct
     3841 accactttca ccaagattgt gaataagtgg aatacagctc tcattggcct tatgacatac
     3901 tttcgagagg ctgtggtgaa tacacaggaa ctcctagact tactggtgaa gtgtgaaaac
     3961 aagatccaga cacgtatcaa gattggattg aactccaaga tgccaagtcg gttcccccct
     4021 gttgtattct acacccctaa ggagctgggt gggcttggca tgctctcgat gggccatgtc
     4081 cttatccccc agtctgacct caggtggtcc aagcaaacag atgtgggcat cacacacttt
     4141 cgttcaggaa tgagtcatga agaagaccaa ctgattccca acttgtaccg ctacatacag
     4201 ccatgggaga gtgaattcat tgactcccag cgggtctggg ctgagtatgc actcaagcga
     4261 caggaggcta ttgctcagaa cagacgcttg actttagaag atttagaaga ctcgtgggat
     4321 cggggcattc cccggatcaa taccctcttc cagaaagatc ggcacacatt ggcttatgat
     4381 aaaggctggc gtgtccgaac tgactttaag cagtaccagg ttttgaagca gaaccccttt
     4441 tggtggacac atcaacggca tgatggcaag ctttggaacc tcaacaacta ccgcacagac
     4501 atgatccagg cactgggcgg tgtggagggc attctggaac acactctctt caagggcact
     4561 tacttcccaa cctgggaagg tctgttctgg gagaaagcca gcgggtttga ggaatccatg
     4621 aagtggaaga agctgaccaa tgctcagcga tcaggattaa accagattcc taatcgtaga
     4681 tttactctct ggtggtcccc aacaatcaat cgagccaatg tatacgtagg cttccaggtg
     4741 cagctggacc tgacaggaat ctttatgcat ggcaagatcc ccacgctgaa gatctctctc
     4801 atccagatct tccgagctca cttgtggcaa aagattcatg agagcatcgt gatggacttg
     4861 tgtcaggtct tcgaccagga attagatgcc ctggaaattg agacagtaca aaaggagaca
     4921 atccatcccc gaaaatccta caagatgaac tcttcctgtg cagatatact gctctttgct
     4981 tcctataagt ggaatgtctc ccggccctcg ctgctggcag actctaagga tgtgatggac
     5041 agcaccacca cacagaagta ttggattgac atccagttac gctgggggga ctatgattcc
     5101 catgatattg agcgctatgc ccgggccaag ttcctggatt acaccacaga caacatgagt
     5161 atctaccctt cacccacagg tgtgctcatt gccattgatc tggcctataa tctacacagt
     5221 gcctatggaa actggttccc ggggagcaag cctctcatac agcaagccat ggccaagatc
     5281 atgaaggcaa accctgcctt gtatgtgtta cgtgaacgga tacgcaaagg attgcagctg
     5341 tattcatctg agcccactga gccttacctg tcctctcaga actatggtga actcttctcg
     5401 aaccagatta tctggtttgt ggatgacacc aatgtctata gagtaactat ccacaagacc
     5461 tttgagggga acttgacaac caaacccatc aatggggcca tcttcatctt caatccacgc
     5521 acaggacagc tgttcctcaa gataatccac acatccgtat gggctggaca gaagcgtttg
     5581 ggtcagttgg ccaagtggaa gactgctgaa gaagtggctg ccctgatccg atctctgcct
     5641 gtggaggaac agcctaaaca gattattgtc actcggaaag ggatgctgga tcccctggag
     5701 gtacacttac tggacttccc caatattgtg atcaaaggat cagagctcca gctgcccttc
     5761 caggcttgtc tcaaggtgga gaagtttggg gatctcatcc ttaaagccac agaaccacag
     5821 atggttctct tcaacctcta tgatgactgg ctcaagacta tctcatccta cacggctttc
     5881 tcccgtctca tcctgatcct gcgtgctttg catgtgaaca acgatagggc aaaagtgatc
     5941 ctgaagccag acaagacaac cgttacggaa ccccaccaca tctggcccac gctcactgac
     6001 gaggagtgga tcaaggtgga ggtgcagctc aaggatctga tcttggctga ctatggcaag
     6061 aaaaacaatg tgaatgtggc atcgctgaca caatcagaaa ttcgagacat catcctggga
     6121 atggagatct ctgcaccatc gcagcagcgg cagcagatag ctgagattga gaagcagacc
     6181 aaggaacagt cccagctcac tgctacacag actcgaactg tcaacaagca tggcgatgag
     6241 atcatcacct ccaccactag caactatgag acccagacct tctcgtcgaa gaccgaatgg
     6301 agagtcaggg ccatctctgc tgccaacctg catctacgga ccaatcacat ttatgtttca
     6361 tctgatgaca tcaaggagac tggttatacg tacattcttc ccaaaaatgt gctgaagaag
     6421 ttcatctgca tatctgatct gcgcgcccaa attgcaggat acctatatgg ggtgagccct
     6481 ccagataacc ctcaggtgaa ggagatccgc tgtattgtga tggtgccaca gtggggcact
     6541 caccagactg tgcacctgcc tagtcagctg cctcaacatg agtacctcaa ggagatggaa
     6601 cccttaggtt ggatccacac tcaacccaat gaatcccccc agctatcgcc ccaagatgta
     6661 actactcatg ccaaaatcat ggctgacaac ccatcttggg atggggagaa gactattatc
     6721 atcacctgca gcttcacacc aggttcctgc acactgacag cctacaagct gacccctagc
     6781 gggtatgagt ggggtcggca gaacacagac aaaggcaaca accccaaagg ctacctacct
     6841 tcgcactatg agagggttca gatgctcctg tctgaccgat tcctcggctt ctttatggtc
     6901 cctgcccagt cctcctggaa ctacaacttt atgggtgttc gacatgaccc caacatgaaa
     6961 tacgagctgc agctagcaaa ccccaaggag ttctaccatg aggtccacag gccctcccac
     7021 ttcctcaact ttgccctcct gcaggagggt gaggtctatt ccgcagacag agaggacctc
     7081 tatgcctagt tacttgcttc tgcttcagat tctccaaggc tggagcctct caaatgccct
     7141 cccacagaca agctgctgac attcagcagc ctgccccttt gtgttttgtg cttgtgttgt
     7201 tgatacattg taatcctgaa caaattaata aattttataa atagga
//
