LOCUS       NM_001024263            5769 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus protein kinase D3 (Prkd3), mRNA.
ACCESSION   NM_001024263 XM_233808
VERSION     NM_001024263.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Wang,J., Sinnett-Smith,J., Stevens,J.V., Young,S.H. and
            Rozengurt,E.
  TITLE     Biphasic Regulation of Yes-associated Protein (YAP) Cellular
            Localization, Phosphorylation, and Activity by G Protein-coupled
            Receptor Agonists in Intestinal Epithelial Cells: A NOVEL ROLE FOR
            PROTEIN KINASE D (PKD)
  JOURNAL   J Biol Chem 291 (34), 17988-18005 (2016)
   PUBMED   27369082
  REMARK    GeneRIF: siRNA-mediated knockdown of PKD1, PKD2, and PKD3 markedly
            attenuated YAP nuclear-cytoplasmic shuttling, phosphorylation at
            Ser(127), and induction of Ctgf and Areg expression in response to
            GPCR activation.
REFERENCE   2  (bases 1 to 5769)
  AUTHORS   Pusapati,G.V., Eiseler,T., Rykx,A., Vandoninck,S., Derua,R.,
            Waelkens,E., Van Lint,J., von Wichert,G. and Seufferlein,T.
  TITLE     Protein kinase D regulates RhoA activity via rhotekin
            phosphorylation
  JOURNAL   J Biol Chem 287 (12), 9473-9483 (2012)
   PUBMED   22228765
REFERENCE   3  (bases 1 to 5769)
  AUTHORS   Li,C., Li,J., Cai,X., Sun,H., Jiao,J., Bai,T., Zhou,X.W., Chen,X.,
            Gill,D.L. and Tang,X.D.
  TITLE     Protein kinase D3 is a pivotal activator of pathological cardiac
            hypertrophy by selectively increasing the expression of
            hypertrophic transcription factors
  JOURNAL   J Biol Chem 286 (47), 40782-40791 (2011)
   PUBMED   21971046
  REMARK    GeneRIF: PKD3 is a pivotal mediator of the
            CaN-NFATc1/c3-PKD3-NFATc4 hypertrophic signaling cascade and a
            potential new drug target for the pathological cardiac hypertrophy
REFERENCE   4  (bases 1 to 5769)
  AUTHORS   Lu,G., Chen,J., Espinoza,L.A., Garfield,S., Toshiyuki,S., Akiko,H.,
            Huppler,A. and Wang,Q.J.
  TITLE     Protein kinase D 3 is localized in vesicular structures and
            interacts with vesicle-associated membrane protein 2
  JOURNAL   Cell Signal 19 (4), 867-879 (2007)
   PUBMED   17196367
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000006.1.
            
            On May 30, 2008 this sequence version replaced NM_001024263.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643292.25006.1,
                                           SRR26643290.21915.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-889               JAXUCZ010000006.1  21792052-21792940
            890-1028            JAXUCZ010000006.1  21823389-21823527
            1029-1160           JAXUCZ010000006.1  21825133-21825264
            1161-1318           JAXUCZ010000006.1  21826856-21827013
            1319-1511           JAXUCZ010000006.1  21832019-21832211
            1512-1589           JAXUCZ010000006.1  21832736-21832813
            1590-1773           JAXUCZ010000006.1  21834995-21835178
            1774-1897           JAXUCZ010000006.1  21836103-21836226
            1898-1975           JAXUCZ010000006.1  21839653-21839730
            1976-2252           JAXUCZ010000006.1  21841038-21841314
            2253-2305           JAXUCZ010000006.1  21844967-21845019
            2306-2378           JAXUCZ010000006.1  21846438-21846510
            2379-2485           JAXUCZ010000006.1  21848462-21848568
            2486-2647           JAXUCZ010000006.1  21851983-21852144
            2648-2746           JAXUCZ010000006.1  21852901-21852999
            2747-3014           JAXUCZ010000006.1  21854773-21855040
            3015-3100           JAXUCZ010000006.1  21857254-21857339
            3101-4051           JAXUCZ010000006.1  21857932-21858882
            4052-4054           JAXUCZ010000006.1  21858883-21858885
            4055-5769           JAXUCZ010000006.1  21858886-21860600
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q12"
     gene            1..5769
                     /gene="Prkd3"
                     /gene_synonym="Prkcn"
                     /note="protein kinase D3"
                     /db_xref="GeneID:313834"
                     /db_xref="RGD:1310236"
     exon            1..889
                     /gene="Prkd3"
                     /gene_synonym="Prkcn"
                     /inference="alignment:Splign:2.1.0"
     CDS             602..3274
                     /gene="Prkd3"
                     /gene_synonym="Prkcn"
                     /EC_number="2.7.11.13"
                     /note="protein kinase C, nu"
                     /codon_start=1
                     /product="serine/threonine-protein kinase D3"
                     /protein_id="NP_001019434.2"
                     /db_xref="GeneID:313834"
                     /db_xref="RGD:1310236"
                     /translation="MSANNSPPSAQKSVFPATVSAVLPTPSPCSSPKTGLSARLSNGS
                     FSAASLTNSRGSVHTVSFLLQIGLTRESVTIEAQELSLSAVKDLVCSIVYQKFPECGF
                     FGMYDKILLFRHNMNSENILQLITSADEIHEGDLVEVVLSALATVEDFQIRPHALYVH
                     SYKAPTFCDYCGEMLWGLVRQGLKCEGCGLNYHKRCAFKIPNNCSGVRKRRLSNVSLP
                     GPGLSVPRPLQPECVPLLSEESHTHQEPSKRIPSWSGRPIWMEKMVMCRVKVPHTFAV
                     HSYGRPTICQYCKRLLKGLFRQGLQCKDCKFNCHKRCASKVPRDCLGEVTFNGEPSSV
                     GTDTDMPMDIDSNDVNSDGSRGLDDSEEPSPPEDKMFFLDAADLDVERDEEPVKTISP
                     STSNNIPLMRVVQSIKHTKRKSSTVVKEGWMVHYTSRDNLRKRHYWRLDSKCLTLFQN
                     ESGSKYYKEIPLSEILRISSPQDFTSISQGSNPHCFEIITDTVVYFVGENNGNSTHNP
                     VLAATGVGLDVAQSWEKAIRQALMPVTPQASVCTSPGQGKDHKDLATSISVSNCQIQE
                     NVDISSVYQIFADEVLGSGQFGIVYGGKHRKTGRDVAIKVIDKMRFPTKQESQLRNEV
                     AILQNLHHPGIVNLECMFETPERVFVVMEKLHGDMLEMILSSEKSRLPERITKFMVTQ
                     ILVALRNLHFKNIVHCDLKPENVLLASAEPFPQVKLCDFGFARIIGEKSFRRSVVGTP
                     AYLAPEVLRSKGYNRSLDMWSVGVIVYVSLSGTFPFNEDEDINDQIQNAAFMYPPNPW
                     REISSEAIDLINNLLQVKMRKRYSVDKSLSHPWLQDYQTWLDLREFETRIGERYITHE
                     SDDARWEIHAYTHNLEYPKHFIMAPNPDDMEEGP"
     exon            890..1028
                     /gene="Prkd3"
                     /gene_synonym="Prkcn"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1160
                     /gene="Prkd3"
                     /gene_synonym="Prkcn"
                     /inference="alignment:Splign:2.1.0"
     exon            1161..1318
                     /gene="Prkd3"
                     /gene_synonym="Prkcn"
                     /inference="alignment:Splign:2.1.0"
     exon            1319..1511
                     /gene="Prkd3"
                     /gene_synonym="Prkcn"
                     /inference="alignment:Splign:2.1.0"
     exon            1512..1589
                     /gene="Prkd3"
                     /gene_synonym="Prkcn"
                     /inference="alignment:Splign:2.1.0"
     exon            1590..1773
                     /gene="Prkd3"
                     /gene_synonym="Prkcn"
                     /inference="alignment:Splign:2.1.0"
     exon            1774..1897
                     /gene="Prkd3"
                     /gene_synonym="Prkcn"
                     /inference="alignment:Splign:2.1.0"
     exon            1898..1975
                     /gene="Prkd3"
                     /gene_synonym="Prkcn"
                     /inference="alignment:Splign:2.1.0"
     exon            1976..2252
                     /gene="Prkd3"
                     /gene_synonym="Prkcn"
                     /inference="alignment:Splign:2.1.0"
     exon            2253..2305
                     /gene="Prkd3"
                     /gene_synonym="Prkcn"
                     /inference="alignment:Splign:2.1.0"
     exon            2306..2378
                     /gene="Prkd3"
                     /gene_synonym="Prkcn"
                     /inference="alignment:Splign:2.1.0"
     exon            2379..2485
                     /gene="Prkd3"
                     /gene_synonym="Prkcn"
                     /inference="alignment:Splign:2.1.0"
     exon            2486..2647
                     /gene="Prkd3"
                     /gene_synonym="Prkcn"
                     /inference="alignment:Splign:2.1.0"
     exon            2648..2746
                     /gene="Prkd3"
                     /gene_synonym="Prkcn"
                     /inference="alignment:Splign:2.1.0"
     exon            2747..3014
                     /gene="Prkd3"
                     /gene_synonym="Prkcn"
                     /inference="alignment:Splign:2.1.0"
     exon            3015..3100
                     /gene="Prkd3"
                     /gene_synonym="Prkcn"
                     /inference="alignment:Splign:2.1.0"
     exon            3101..5769
                     /gene="Prkd3"
                     /gene_synonym="Prkcn"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ttttctctct gggtccattc atccaaagaa atatgtttgt gctatttgac aagctttttg
       61 ttttcacctc atcacaggga agttcagcca caagatggaa atgagtacag atgagagggt
      121 gaattaaggt gtgatcattt gtggtgtccc gtcaatccct aaaggaatag attacctgga
      181 actactttta atcaggaaga tttcatatct tggcacagat tgactattca atttgtattt
      241 tctgaaatgc aaagcaaaag ataagaacag atttaaaaga aatgaagatg tcgcataaat
      301 aatcagtatc aactctgttc ctttgatgat aatggtgatg tgaagtgggt tacagatctg
      361 ggtgtggtcc taaagacaag attcttgatg ggactgacat tgacagcagt aggacctttt
      421 gtgcattcaa ctcggagcaa cagaatttgt catcaacagc atgagtggct cagccaggga
      481 aagaaagcag ctttcatcta aatccaaacc atagccattt ttttttactg ctattttgaa
      541 aacttttcca gaaccattta aaaagcattg acaactattt ttatagatta aagaaaggca
      601 gatgtctgca aataattccc ctccatcagc ccagaaatct gtgttccctg caactgtttc
      661 tgctgtgctt ccaacccctt ctccgtgttc aagtcccaag acaggactct cagctcggct
      721 ctctaatggc agcttcagtg ccgcgtcact cacaaactct agaggctcgg tgcacaccgt
      781 ttcatttcta cttcagattg gcctcacacg agagagtgtt accatcgaag cccaggaact
      841 gtctctctcc gctgtcaaag acctcgtgtg ctccattgtt tatcagaagt ttccagagtg
      901 tggattcttt ggcatgtatg ataaaattct tctctttcgc cacaacatga actcagaaaa
      961 cattttgcag ctgattacct cagcagatga aatacatgaa ggggatcttg tagaagttgt
     1021 cctgtcagct ttggccacag tagaagactt ccagatccgc cctcatgctc tctatgtcca
     1081 ttcttacaaa gcacctactt tctgtgatta ctgtggtgaa atgctctggg gattggtacg
     1141 tcaaggatta aaatgtgaag gctgtggatt aaattatcat aaacgatgtg ccttcaagat
     1201 tccaaataac tgcagtggag taagaaagag acgtctgtca aatgtgtctc tgccaggacc
     1261 tggcctctca gttccgagac ccctgcagcc tgaatgtgtg cccctgctca gtgaagagtc
     1321 acatactcac caagagccaa gtaagagaat cccgtcctgg agtggtcgtc cgatctggat
     1381 ggaaaagatg gtaatgtgca gggtcaaagt tccacacacg tttgctgtcc actcttatgg
     1441 ccgacccaca atatgtcagt actgcaagcg gctgctcaaa ggcctctttc gccaaggact
     1501 gcagtgtaaa gattgcaaat tcaactgcca caaacgctgt gcatccaaag taccacgaga
     1561 ctgcctcggc gaagttactt tcaatggaga gccttccagt gtgggaactg atacagacat
     1621 gccgatggat attgacagta atgatgtgaa cagtgatggc agtaggggct tggatgactc
     1681 agaggagccc tcccctcccg aagacaaaat gttcttcctc gacgcggccg atctggatgt
     1741 ggagcgagat gaggagccgg ttaagacaat cagtccatcc acaagcaata atatcccact
     1801 gatgagggtg gtccagtcca tcaagcacac aaagaggaag agcagcacag tggtgaagga
     1861 agggtggatg gtccactaca ccagcaggga caacctgaga aagagacatt attggagact
     1921 tgacagcaaa tgtctaacat tgtttcagaa tgaatctgga tcaaaatatt ataaggaaat
     1981 tccactttca gaaattctcc gcatatcttc gccacaagat ttcaccagca tttcacaagg
     2041 cagtaaccca cactgttttg aaatcatcac tgatactgtg gtatactttg ttggtgagaa
     2101 caatgggaac agcactcata accctgttct cgctgccact ggagtgggac tcgatgtagc
     2161 acaaagctgg gaaaaagcga tccgccaggc cctcatgccc gttactcctc aagcaagtgt
     2221 ttgcacttct ccaggccagg ggaaggacca caaagatctg gctaccagta tctccgtgtc
     2281 taactgtcag attcaggaga atgtagacat cagcagtgtt tatcagatct ttgccgatga
     2341 ggtgcttggt tctggccagt ttggcattgt ttatggagga aagcacagaa agacaggaag
     2401 ggatgtggct attaaagtaa tcgataagat gcgattcccc acaaagcaag agagtcagct
     2461 ccggaatgaa gtggctattt tacagaatct gcaccatcct gggattgtaa atctggaatg
     2521 tatgtttgaa accccagaac gagtcttcgt agtgatggaa aagcttcatg gagatatgtt
     2581 ggaaatgatt ctgtccagtg aaaaaagtcg acttccagaa cggattacta aattcatggt
     2641 cacacagata cttgttgccc tgaggaatct acattttaag aatatcgtgc actgtgattt
     2701 aaagcccgaa aatgtgctgc ttgcatctgc agaacctttt cctcaggtga agctatgtga
     2761 ctttgggttt gcccgcatca ttggtgagaa gtcgttccgg aggtcagtgg tgggaactcc
     2821 agcctactta gcccccgagg ttctcaggag caaaggctac aaccgttccc tagacatgtg
     2881 gtcggtggga gtcatagtct atgtgagcct cagtggcacc ttcccgttca atgaagatga
     2941 agatataaac gaccaaatcc aaaatgctgc atttatgtac ccaccaaatc catggcgaga
     3001 aatctccagc gaagcaattg atttgataaa caacttgctt caagtgaaga tgagaaaacg
     3061 ttacagcgtt gacaagtccc tcagtcatcc ctggctacag gactatcaga cttggcttga
     3121 cctcagagaa ttcgaaactc gcattggaga acgttacatc acacatgaaa gtgatgatgc
     3181 tcgctgggaa atacatgcat acacgcacaa tctcgaatac ccaaaacatt tcattatggc
     3241 ccccaacccg gatgacatgg aggaaggtcc ttagttgtga gtgggctaat cctataagga
     3301 agggtttcat ttcaagacgg atactttgct gcatgactgt tgctctctgc agactgtcat
     3361 tgcaaggcgg cagcatcatg aggacataga aggacgtgct ggcatcaatt ctgtagctca
     3421 taacgagtag gtacatgaag ggaaactgag taagaaattc aaaatggaac ttgttataaa
     3481 tttttatagc ataagcaata actggttctg taatttccta atccttcaca ttagcacaat
     3541 gtgaactagc acctaattta tctctccttt gctgttatta gatcatttgt tttaaaggcc
     3601 tatgactgtc tagctattgt ttataaggtt gattactcaa caggtttgca gttaagcagt
     3661 aaccacgagg ctaacaacat atggtttgct gtaaggactt ttagtatttg tccactgttg
     3721 gaggcattga ttcttcctgg acccctacaa acagaacact gcactgctcc ccttgtggtg
     3781 gatgcctctt tacatacggt ctgtgtgaca taagtcgtga ctctttccat gtcgtagtag
     3841 tcactagaac tgaacagttg gtgttacaaa tgagtagaac tagctggtga gcactcggct
     3901 gtgccactga gcacgtatat tctcaattga caccatctta atatggtgtg ccaacagtgt
     3961 gtgggcacaa ctctgcattt aactaattta tccttacctg gagctgaata aattatagac
     4021 aaaattacat actaaagagg aaagctagta cggggtcagt aaatgtcact acacaacaag
     4081 cagtatcatt tccttcttcc cttagccttt gggtgggatt tgcaaatatc tcgtttttta
     4141 aattagcatt gtattttttt ttttttttgg ttcttttttt tcggagctgg ggaccgaacc
     4201 cagggccttg cgcttcctag gcaagtgctc taccactgag ctaaatcccc aacccctagc
     4261 attgtatttt tagacggttt ggaggagtgg ttgaagtcag acaggaaagt ccagcttatc
     4321 gggagtttac cagtacagcg gatgaaggaa gagtaagaac atcctacact tcagctccag
     4381 agccagccgt tttgtagtag ttattaatac aggttcagta tccctatttc cgctgctcta
     4441 caccgggagg gcttcagttt ccagatactg aaatgtttgc agactctact agttgagcat
     4501 cctgacttac aaatctgaaa ccccccaaat tcaaaaacct ttgagttctg tgcccttcag
     4561 atttctacat tagcgatagt cccatctgtg ctttacccaa agaaaaggat ccaggactat
     4621 ctgcttgtga gaaatatagt agctgtttgt ttcttcattc atcactagtc accaagaaca
     4681 gtttttctta tttctccctt tctgtgctaa aagcaaaaat ttggaatcag aaaacctgag
     4741 tttaagtttt agtgtctgta ctctgtagat ctgtttggct gagcaggtga cagtttgtct
     4801 gagcacttag tttctcagtc tggagagtgg acaccataaa ttcctactgc ctctttcaat
     4861 gtggctgtga agattgggct gtgtgaccgc ttctgacaat ctggaacgcc agtgtcgtca
     4921 cttcccactg gtcccgagat agcaaaggaa aaagttggtg tgtgaacaga ctaaaactaa
     4981 gtgccctggc aggtgagaga gcagctgcag gtatctttct gacatgtaaa atgtccttga
     5041 agaaacacag acattagggg tgcctctgtt gtaaatgttt atgctttaaa ctgtcctcac
     5101 attaggctaa gcacacttaa agaagttgag gcacacggtt agctgttgaa ggtcactgag
     5161 taaatatgga tttaaaccga ggccttcatg tcccttcaac cgtcccagcc attggcaatg
     5221 tactgtatat ctgtaagcat ttccagaaga ctttccaaac tgtctctgtg gttgttttca
     5281 tacagcagtt gtgaagagta attttaaaaa acttgtaaga atggattttt catttctgct
     5341 gagtttgctt gaccagtatg tacaagatgg agctagtgtg tgtcactagc ccactgtggc
     5401 tttactgtgt gtgtggaaac taactggagt gactgctggg ttgctgtacc tctgtaatca
     5461 gactttacaa gccatgacat taattgcact ctgttttcgt tgaagcccta tgctgtttaa
     5521 agagagactg taaaggcact gtcgtagttg ggagttgagt aatactccac tgtgtaacct
     5581 gcataacttt tgtcattgtc ttaaagccaa gctgtgtttg cataaaaagt aattgtttct
     5641 tagaaacatg tgacaatttt caaagtagtg tataatgaca ctgtttagta cattttatat
     5701 tttttgttac atcccacttt tgtaaatatt cttaaaactt gataataaaa tgtatttctc
     5761 tatcaccat
//
