LOCUS       NM_001030020            3182 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus protein kinase cAMP-dependent type II regulatory
            subunit beta (Prkar2b), mRNA.
ACCESSION   NM_001030020 XM_001073451 XM_001073478 XM_001073507 XM_001073542
            XM_001073576 XM_343046
VERSION     NM_001030020.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Zhang,P., Knape,M.J., Ahuja,L.G., Keshwani,M.M., King,C.C.,
            Sastri,M., Herberg,F.W. and Taylor,S.S.
  TITLE     Single Turnover Autophosphorylation Cycle of the PKA RIIbeta
            Holoenzyme
  JOURNAL   PLoS Biol 13 (7), e1002192 (2015)
   PUBMED   26158466
  REMARK    GeneRIF: Release of pRIIbeta in the presence of cAMP is reduced by
            calcium, whereas autophosphorylation at the phosphorylation site
            inhibits holoenzyme reassociation with the catalytic subunit.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Blumenthal,D.K., Copps,J., Smith-Nguyen,E.V., Zhang,P., Heller,W.T.
            and Taylor,S.S.
  TITLE     The roles of the RIIbeta linker and N-terminal cyclic
            nucleotide-binding domain in determining the unique structures of
            the type IIbeta protein kinase A: a small angle x-ray and neutron
            scattering study
  JOURNAL   J Biol Chem 289 (41), 28505-28512 (2014)
   PUBMED   25112875
  REMARK    GeneRIF: The unique structural properties of RiIbeta do not require
            the C-terminal cAMP-binding domain.
REFERENCE   3  (bases 1 to 3182)
  AUTHORS   Parisiadou,L., Yu,J., Sgobio,C., Xie,C., Liu,G., Sun,L., Gu,X.L.,
            Lin,X., Crowley,N.A., Lovinger,D.M. and Cai,H.
  TITLE     LRRK2 regulates synaptogenesis and dopamine receptor activation
            through modulation of PKA activity
  JOURNAL   Nat Neurosci 17 (3), 367-376 (2014)
   PUBMED   24464040
  REMARK    Erratum:[Nat Neurosci. 2014 Sep;17(9):1287]
REFERENCE   4  (bases 1 to 3182)
  AUTHORS   Zhang,P., Smith-Nguyen,E.V., Keshwani,M.M., Deal,M.S., Kornev,A.P.
            and Taylor,S.S.
  TITLE     Structure and allostery of the PKA RIIbeta tetrameric holoenzyme
  JOURNAL   Science 335 (6069), 712-716 (2012)
   PUBMED   22323819
  REMARK    GeneRIF: study describes the 2.3 angstrom structure of full-length
            tetrameric RIIbeta(2):C(2) holoenzyme
REFERENCE   5  (bases 1 to 3182)
  AUTHORS   Tuson,M., He,M. and Anderson,K.V.
  TITLE     Protein kinase A acts at the basal body of the primary cilium to
            prevent Gli2 activation and ventralization of the mouse neural tube
  JOURNAL   Development 138 (22), 4921-4930 (2011)
   PUBMED   22007132
REFERENCE   6  (bases 1 to 3182)
  AUTHORS   Cummings,D.E., Brandon,E.P., Planas,J.V., Motamed,K., Idzerda,R.L.
            and McKnight,G.S.
  TITLE     Genetically lean mice result from targeted disruption of the RII
            beta subunit of protein kinase A
  JOURNAL   Nature 382 (6592), 622-626 (1996)
   PUBMED   8757131
REFERENCE   7  (bases 1 to 3182)
  AUTHORS   Greene,R.M., Lloyd,M.R., Uberti,M., Nugent,P. and Pisano,M.M.
  TITLE     Patterns of cyclic AMP-dependent protein kinase gene expression
            during ontogeny of the murine palate
  JOURNAL   J Cell Physiol 163 (3), 431-440 (1995)
   PUBMED   7775586
REFERENCE   8  (bases 1 to 3182)
  AUTHORS   Kurten,R.C., Levy,L.O., Shey,J., Durica,J.M. and Richards,J.S.
  TITLE     Identification and characterization of the GC-rich and cyclic
            adenosine 3',5'-monophosphate (cAMP)-inducible promoter of the type
            II beta cAMP-dependent protein kinase regulatory subunit gene
  JOURNAL   Mol Endocrinol 6 (4), 536-550 (1992)
   PUBMED   1316546
REFERENCE   9  (bases 1 to 3182)
  AUTHORS   Sandberg,M., Levy,F.O., Oyen,O., Hansson,V. and Jahnsen,T.
  TITLE     Molecular cloning, cDNA structure and deduced amino acid sequence
            for the hormone-induced regulatory subunit (RII beta) of
            cAMP-dependent protein kinase from rat ovarian granulosa cells
  JOURNAL   Biochem Biophys Res Commun 154 (2), 705-711 (1988)
   PUBMED   3401231
REFERENCE   10 (bases 1 to 3182)
  AUTHORS   Jahnsen,T., Hedin,L., Kidd,V.J., Beattie,W.G., Lohmann,S.M.,
            Walter,U., Durica,J., Schulz,T.Z., Schiltz,E., Browner,M. et al.
  TITLE     Molecular cloning, cDNA structure, and regulation of the regulatory
            subunit of type II cAMP-dependent protein kinase from rat ovarian
            granulosa cells
  JOURNAL   J Biol Chem 261 (26), 12352-12361 (1986)
   PUBMED   2427518
  REMARK    Erratum:[J Biol Chem 1988 Mar 15;263(8):4041]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CB814252.1, M12492.1, CX569141.1 and BI298837.1.
            
            On or before Oct 11, 2007 this sequence version replaced
            XM_343046.3, XM_001073478.1, XM_001073542.1, XM_001073576.1,
            XM_001073507.1, XM_001073451.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN12840110
                              [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 CB814252.1         232-237
            7-1649              M12492.1           1-1643
            1650-2725           M12492.1           1645-2720
            2726-3086           CX569141.1         385-745
            3087-3182           BI298837.1         1-96                c
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q16"
     gene            1..3182
                     /gene="Prkar2b"
                     /gene_synonym="RATDNA"
                     /note="protein kinase cAMP-dependent type II regulatory
                     subunit beta"
                     /db_xref="GeneID:24679"
                     /db_xref="RGD:3394"
     exon            1..306
                     /gene="Prkar2b"
                     /gene_synonym="RATDNA"
                     /inference="alignment:Splign:2.1.0"
     CDS             6..1256
                     /gene="Prkar2b"
                     /gene_synonym="RATDNA"
                     /EC_number="2.7.11.1"
                     /note="Type II beta regulatory subunit of cAMP-dependent
                     protein kinase; protein kinase, cAMP dependent regulatory,
                     type II beta; protein kinase, cAMP-dependent, regulatory
                     subunit type II beta; protein kinase cAMP-dependent type 2
                     regulatory subunit beta"
                     /codon_start=1
                     /product="cAMP-dependent protein kinase type II-beta
                     regulatory subunit"
                     /protein_id="NP_001025191.1"
                     /db_xref="GeneID:24679"
                     /db_xref="RGD:3394"
                     /translation="MSIEIPAGLTELLQGFTVEVLRHQPADLLEFALQHFTRLQQENE
                     RKGAARFGHEGRTWGDAGAAAGGGTPSKGVNFAEEPMRSDSENGEEEEAAEAGAFNAP
                     VINRFTRRASVCAEAYNPDEEEDDAESRIIHPKTDDQRNRLQEACKDILLFKNLDPEQ
                     MSQVLDAMFEKLVKEGEHVIDQGDDGDNFYVIDRGTFDIYVKCDGVGRCVGNYDNRGS
                     FGELALMYNTPRAATITATSPGALWGLDRVTFRRIIVKNNAKKRKMYESFIESLPFLK
                     SLEVSERLKVVDVIGTKVYNDGEQIIAQGDSADSFFIVESGEVRITMKRKGKSDIEEN
                     GAVEIARCLRGQYFGELALVTNKPRAASAHAIGTVKCLAMDVQAFERLLGPCMEIMKR
                     NIATYEEQLVALFGTNMDIVEPTA"
     exon            307..342
                     /gene="Prkar2b"
                     /gene_synonym="RATDNA"
                     /inference="alignment:Splign:2.1.0"
     exon            343..395
                     /gene="Prkar2b"
                     /gene_synonym="RATDNA"
                     /inference="alignment:Splign:2.1.0"
     exon            396..479
                     /gene="Prkar2b"
                     /gene_synonym="RATDNA"
                     /inference="alignment:Splign:2.1.0"
     exon            480..586
                     /gene="Prkar2b"
                     /gene_synonym="RATDNA"
                     /inference="alignment:Splign:2.1.0"
     exon            587..740
                     /gene="Prkar2b"
                     /gene_synonym="RATDNA"
                     /inference="alignment:Splign:2.1.0"
     exon            741..842
                     /gene="Prkar2b"
                     /gene_synonym="RATDNA"
                     /inference="alignment:Splign:2.1.0"
     exon            843..917
                     /gene="Prkar2b"
                     /gene_synonym="RATDNA"
                     /inference="alignment:Splign:2.1.0"
     exon            918..983
                     /gene="Prkar2b"
                     /gene_synonym="RATDNA"
                     /inference="alignment:Splign:2.1.0"
     exon            984..1122
                     /gene="Prkar2b"
                     /gene_synonym="RATDNA"
                     /inference="alignment:Splign:2.1.0"
     exon            1123..3170
                     /gene="Prkar2b"
                     /gene_synonym="RATDNA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1561..1566
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prkar2b"
                     /gene_synonym="RATDNA"
     regulatory      1813..1818
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Prkar2b"
                     /gene_synonym="RATDNA"
ORIGIN      
        1 gcaggatgag catcgagatc cccgcggggc tcacggagct gctgcagggc ttcacggtgg
       61 aggtgctgag gcaccagccc gccgacctgc tggagttcgc gctgcagcac ttcacgcggc
      121 tgcagcagga gaacgagcgc aagggcgccg cgcgcttcgg ccatgagggc aggacctggg
      181 gggacgcggg cgcagccgcc gggggcggaa cccccagtaa gggtgtcaac ttcgcggagg
      241 agcccatgcg ctccgattcc gagaacggcg aagaggagga ggccgcggaa gcaggggcgt
      301 tcaacgctcc agttataaac cggttcacaa ggcgtgcctc ggtatgtgca gaagcttata
      361 atcctgatga agaagaagat gatgcagagt ccaggataat acatcccaaa actgacgatc
      421 aaagaaacag attgcaagaa gcctgcaaag acatcctgct gtttaagaac ctggatccag
      481 aacagatgtc tcaagtatta gatgccatgt ttgaaaaatt ggtcaaagaa ggggaacacg
      541 taatcgatca aggtgatgat ggtgacaact tttacgtcat cgacagagga acatttgata
      601 tttatgtaaa atgtgatggc gttggaagat gcgttggtaa ctatgacaat cgtgggagtt
      661 ttggagaact ggccttaatg tacaatacac ccagagcagc tacaatcact gctacctctc
      721 ctggtgctct gtggggtttg gacagggtga ccttcaggag aataatagta aaaaacaatg
      781 ccaaaaagag gaagatgtac gagagcttta ttgagtcact gccattcctc aagtctctgg
      841 aggtttctga acgcctgaag gtggtagatg tgattggcac caaagtttac aacgatggag
      901 aacagatcat tgctcaggga gactcggcgg attcgttctt cattgtagaa tctggagaag
      961 tgagaattac tatgaagaga aagggtaaat cagacatcga agagaacggt gctgtggaaa
     1021 tcgctcggtg tctccgggga cagtattttg gagagcttgc cctggtcact aacaagccaa
     1081 gagcagcatc tgcacacgcc attgggactg tcaaatgctt agccatggat gtgcaagcat
     1141 ttgagaggct tctgggacct tgcatggaaa ttatgaagag gaatatcgcc acctatgagg
     1201 agcaattagt tgcactgttt gggacaaaca tggatattgt tgagcccact gcatgaagca
     1261 aaagtgtggg gaagaaagcg cgttagtgaa gcagttacat agcagtggtt agtccaccga
     1321 ggatgtgttc gtgtagatca agcattttct gtgacttcag gttttccttt tttaaacaga
     1381 aaccatttac aatatatcag tgaacagtag tgatttaata gtaggcttta acatcacttt
     1441 ctaatgggta gttcataaat taacataatc ataaaaacga ctttctattt cataaaactg
     1501 actgaaaggt ttattcaaat ggttataata tattgagagc atacatgttt aagaagataa
     1561 ttaaaagatg tactcatagg ccgacctgtg tgtttggatt attcaagtat cgtgttagtg
     1621 actctgcccc tttacgaggg aagctggcag tggcttgtgc actcacattc tttttataac
     1681 tcgtcagaca gtaagattgc caatcatttc attttctacc ttttctggct tagtaagtta
     1741 tgtgttagcc ttggcctatc ggtaacatgg tgtggaaatc atatgcaatt ttaaaatttt
     1801 ttatattttt gcaataaact acatttcaac ttctttgaca actgtcagcc agcagcctgg
     1861 gtagttgagt ggcttttggg gcctggtaat ttagctgttg tgatgattgg gcagagagcc
     1921 ttttaggatg aaagctcata actgcgtctt tcattatcct ttctccatca tcaccgcttc
     1981 ccgctttgta tttagcaacc tcctgggttc cttacagatc gcattttgac gtttaatgtc
     2041 acaagggttg tattggttct caaggcaatt tcatcttaat ctgacaattt gttttgtcct
     2101 ctaaaagtcc cgaatcttct attcagaaaa atgaaagctt ccctatcaaa agacatttgg
     2161 gattcttagc ccacgcccac agaacatggc tttgctctgt aggtcccagt gctgcgaccc
     2221 tgtgtgtgtg tctgtggtcc cctgcccttc cctgtcctga gcaccagcca gcgagcacat
     2281 tggtcagcag ccaatcaagt gcgtttgccc tttccaacct tcaaaaactt caacaacggt
     2341 ggtggaaaga agtctgtgta taagcaaagc catacatgag tattaatggg atttttctct
     2401 atcctacacc ttattaggcc ttgttcttag ctttaaagtc agcccacagt taaaagtaca
     2461 caaagtacaa aatttttttt ggtcaacaag atacttactt ttatgccaaa tacgatgaat
     2521 tgttaaaacc aaagcaatat acttttatgt gattttcagg ccccttgacc aaatgtctaa
     2581 aggcattaat tctcaccagt ttaattcatg ttgaaatggg taattttact ttggggaaca
     2641 tgtttctaag gaacagaggt atcatgtatg ttaaattcat ctgtgactgt ctcgatctta
     2701 caagagaatg tttacagtcc tacgtgtacg tgaaaacgtg gctagtgtta aatgcatcat
     2761 gtgtaaacaa gaagttaaag tacgctatac tgttacctac acctttagcc gtgcttttga
     2821 aatgtcagtc ttgcaaaatc tttacgtagg aaaaaaagct ctctacatga aggtaacatg
     2881 ttttctattt cagagttcag aagccatgtg ttcaaacaag aaacaaatcc caaaaacaga
     2941 aaaacaagaa caaaagccac ccaaattcct ggctcatcct tctgtgttct tttctctctt
     3001 ttccaagttc tctttggttg cattgcatag gctgagtgtt gacgttcacg gaaaccaatg
     3061 cctgtggaat aaagagcccc aaccaaggct acagaggcca gctgtgttat tttcagtatt
     3121 gttggttata tttaaatttt ccttataata aagcacactt ttataataaa aaaaaaaaaa
     3181 aa
//
