LOCUS       NM_022627               1758 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Rattus norvegicus protein kinase AMP-activated non-catalytic
            subunit beta 2 (Prkab2), mRNA.
ACCESSION   NM_022627
VERSION     NM_022627.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Katwan,O.J., Alghamdi,F., Almabrouk,T.A., Mancini,S.J., Kennedy,S.,
            Oakhill,J.S., Scott,J.W. and Salt,I.P.
  TITLE     AMP-activated protein kinase complexes containing the beta2
            regulatory subunit are up-regulated during and contribute to
            adipogenesis
  JOURNAL   Biochem J 476 (12), 1725-1740 (2019)
   PUBMED   31189568
  REMARK    GeneRIF: these data indicate that increased expression of AMPKbeta2
            is an important feature of efficient adipogenesis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Mottillo,E.P., Desjardins,E.M., Crane,J.D., Smith,B.K., Green,A.E.,
            Ducommun,S., Henriksen,T.I., Rebalka,I.A., Razi,A., Sakamoto,K.,
            Scheele,C., Kemp,B.E., Hawke,T.J., Ortega,J., Granneman,J.G. and
            Steinberg,G.R.
  TITLE     Lack of Adipocyte AMPK Exacerbates Insulin Resistance and Hepatic
            Steatosis through Brown and Beige Adipose Tissue Function
  JOURNAL   Cell Metab 24 (1), 118-129 (2016)
   PUBMED   27411013
REFERENCE   3  (bases 1 to 1758)
  AUTHORS   Xu,H., Frankenberg,N.T., Lamb,G.D., Gooley,P.R., Stapleton,D.I. and
            Murphy,R.M.
  TITLE     When phosphorylated at Thr148, the beta2-subunit of AMP-activated
            kinase does not associate with glycogen in skeletal muscle
  JOURNAL   Am J Physiol Cell Physiol 311 (1), C35-C42 (2016)
   PUBMED   27099349
  REMARK    GeneRIF: In rat skeletal muscle, beta2-AMPK is not associated with
            glycogen and that activation of AMPK by muscle contraction does not
            dephosphorylate beta2-AMPK.
REFERENCE   4  (bases 1 to 1758)
  AUTHORS   Klaus,A., Zorman,S., Berthier,A., Polge,C., Ramirez,S.,
            Michelland,S., Seve,M., Vertommen,D., Rider,M., Lentze,N.,
            Auerbach,D. and Schlattner,U.
  TITLE     Glutathione S-transferases interact with AMP-activated protein
            kinase: evidence for S-glutathionylation and activation in vitro
  JOURNAL   PLoS One 8 (5), e62497 (2013)
   PUBMED   23741294
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1758)
  AUTHORS   Wang,J., Huo,K., Ma,L., Tang,L., Li,D., Huang,X., Yuan,Y., Li,C.,
            Wang,W., Guan,W., Chen,H., Jin,C., Wei,J., Zhang,W., Yang,Y.,
            Liu,Q., Zhou,Y., Zhang,C., Wu,Z., Xu,W., Zhang,Y., Liu,T., Yu,D.,
            Zhang,Y., Chen,L., Zhu,D., Zhong,X., Kang,L., Gan,X., Yu,X., Ma,Q.,
            Yan,J., Zhou,L., Liu,Z., Zhu,Y., Zhou,T., He,F. and Yang,X.
  TITLE     Toward an understanding of the protein interaction network of the
            human liver
  JOURNAL   Mol Syst Biol 7, 536 (2011)
   PUBMED   21988832
  REMARK    Erratum:[Mol Syst Biol. 2017 Dec 18;13(12):965. doi:
            10.15252/msb.20178107.. PMID: 29254952]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1758)
  AUTHORS   Hardie,D.G.
  TITLE     The AMP-activated protein kinase pathway--new players upstream and
            downstream
  JOURNAL   J Cell Sci 117 (Pt 23), 5479-5487 (2004)
   PUBMED   15509864
  REMARK    Review article
REFERENCE   7  (bases 1 to 1758)
  AUTHORS   Woods,A., Johnstone,S.R., Dickerson,K., Leiper,F.C., Fryer,L.G.,
            Neumann,D., Schlattner,U., Wallimann,T., Carlson,M. and Carling,D.
  TITLE     LKB1 is the upstream kinase in the AMP-activated protein kinase
            cascade
  JOURNAL   Curr Biol 13 (22), 2004-2008 (2003)
   PUBMED   14614828
REFERENCE   8  (bases 1 to 1758)
  AUTHORS   Hardie,D.G., Scott,J.W., Pan,D.A. and Hudson,E.R.
  TITLE     Management of cellular energy by the AMP-activated protein kinase
            system
  JOURNAL   FEBS Lett 546 (1), 113-120 (2003)
   PUBMED   12829246
  REMARK    Review article
REFERENCE   9  (bases 1 to 1758)
  AUTHORS   Kawaguchi,T., Osatomi,K., Yamashita,H., Kabashima,T. and Uyeda,K.
  TITLE     Mechanism for fatty acid 'sparing' effect on glucose-induced
            transcription: regulation of carbohydrate-responsive
            element-binding protein by AMP-activated protein kinase
  JOURNAL   J Biol Chem 277 (6), 3829-3835 (2002)
   PUBMED   11724780
REFERENCE   10 (bases 1 to 1758)
  AUTHORS   Chen,Z., Heierhorst,J., Mann,R.J., Mitchelhill,K.I., Michell,B.J.,
            Witters,L.A., Lynch,G.S., Kemp,B.E. and Stapleton,D.
  TITLE     Expression of the AMP-activated protein kinase beta1 and beta2
            subunits in skeletal muscle
  JOURNAL   FEBS Lett 460 (2), 343-348 (1999)
   PUBMED   10544261
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AF182717.1.
            
            On Aug 30, 2012 this sequence version replaced NM_022627.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF182717.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1758              AF182717.1         3-1760
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q34"
     gene            1..1758
                     /gene="Prkab2"
                     /note="protein kinase AMP-activated non-catalytic subunit
                     beta 2"
                     /db_xref="GeneID:64562"
                     /db_xref="RGD:620905"
     exon            1..160
                     /gene="Prkab2"
                     /inference="alignment:Splign:2.1.0"
     CDS             8..823
                     /gene="Prkab2"
                     /function="involved in modulating metabolism in response
                     to energy demand and nutrient availability"
                     /note="AMPK beta-2 chain; AMPK subunit beta-2;
                     AMP-activated protein kinase beta-2 regulatory subunit;
                     protein kinase, AMP-activated, beta 2 non-catalytic
                     subunit"
                     /codon_start=1
                     /product="5'-AMP-activated protein kinase subunit beta-2"
                     /protein_id="NP_072149.1"
                     /db_xref="GeneID:64562"
                     /db_xref="RGD:620905"
                     /translation="MGNTTSERVSGERHGAKAARAEGGGHGPGKEHKIMVGSTDDPSV
                     FSLPDSKLPGDKEFVPWQQDLDDSVKPTQQARPTVIRWSEGGKEVFISGSFNNWSTKI
                     PLIKSHNDFVAILDLPEGEHQYKFFVDGQWVHDPSEPVVTSQLGTINNLIHVKKSDFE
                     VFDALKLDSMESSETSCRDLSSSPPGPYGQEMYVFRSEERFKSPPILPPHLLQVILNK
                     DTNISCDPALLPEPNHVMLNHLYALSTKDSVMVLSATHRYKKKYVTTLLYKPI"
     exon            161..327
                     /gene="Prkab2"
                     /inference="alignment:Splign:2.1.0"
     exon            328..421
                     /gene="Prkab2"
                     /inference="alignment:Splign:2.1.0"
     exon            422..542
                     /gene="Prkab2"
                     /inference="alignment:Splign:2.1.0"
     exon            543..676
                     /gene="Prkab2"
                     /inference="alignment:Splign:2.1.0"
     exon            677..745
                     /gene="Prkab2"
                     /inference="alignment:Splign:2.1.0"
     exon            746..1752
                     /gene="Prkab2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tgcagccatg ggaaacacca ccagtgagcg ggtgtccggg gagcgccacg gtgccaaggc
       61 tgctcgagct gagggtggcg gccatggccc cggcaaggag cacaagatca tggtgggaag
      121 caccgacgac cccagcgtct tcagcctgcc ggactccaag ctccctgggg acaaagagtt
      181 tgtaccctgg cagcaggatt tggatgattc tgtgaagccc acccagcagg cccggcctac
      241 tgttatccgc tggtctgaag ggggcaagga ggtcttcatc tctgggtctt tcaacaattg
      301 gagcaccaag atccctctga ttaagagtca taatgacttc gttgccatcc tggatcttcc
      361 cgagggagag caccagtaca agttctttgt ggatggacag tgggttcatg atccatcaga
      421 gcctgtggtt accagtcagc ttggcacaat taataatttg atccatgtca agaaatctga
      481 ttttgaagta tttgatgctt taaagttaga ttctatggaa agctctgaga catcatgtcg
      541 agacctgtcc agctcacccc cagggcctta tggtcaagaa atgtatgtgt ttcgatctga
      601 ggagagattc aaatccccac ccatcctgcc ccctcacctc ctccaagtta ttcttaacaa
      661 ggacactaat atttcatgtg acccagcctt acttcctgag cccaatcatg ttatgctgaa
      721 ccatctctat gcgctgtcca ctaaggacag tgtgatggtc cttagcgcaa cccatcgcta
      781 caagaagaag tacgtcacca cgctgctgta taagcccatc tgaaggagat atcactcctt
      841 gcctccaagg attctggaga agcatctccc ttgcctttga actgagctgg cctcctctga
      901 gcctggcagg ctgtttgttt tgaggctgat gtgtgtgtca gagcctctgg taggagctct
      961 gctttgcctt tgattgcaga cgagagcttt atgagttcat ggagtttatt ttaagaaaca
     1021 caaaaccata tatatgcata tgagaggaag gttaccagaa gcctcctggc ccagctgtcc
     1081 acactctctc tgcctgtggg tagttgccag ggcctgactg ggaagctgtg gctgcaggaa
     1141 tgggtgtacc ggaagcttct ttttctaaag tgagagaata gcaaactctt aggatccttg
     1201 ttgggcagag gtcctggacc actggtgtcc aagggtcaga gtgtgccagg ccactgccct
     1261 acttagagct gcctcgtgcg ggtggctttc cttgcatggg ttgattctgt atcccacact
     1321 gtgtgacaca atccaccgtg gtttatatgg cactagttgc tccacaagaa ccctcctcat
     1381 tttatgtacc ctgtggaatg acttccctca gatctcatac atgacccagt aatgcaaaac
     1441 tcactgggcg aggaaccctt ttgaagagtt tgtgggatga gcctcacctt agagcctgag
     1501 gtgccagggt ctccttttta gctcagtgaa ggcctaaccc tgtgtgttgc agtttacttt
     1561 aacatctaga gaaaagaaat gcacaccgtt gtgtctcggt tggtcctgtg tattggctgt
     1621 gtgggcctgt ctccggtttt ctttatcaga gcactcagct tgtaggcagg cttgcactta
     1681 gggaaacctt ccatatcttt catccctgaa agttttttta tgtgcctaca tttttcttta
     1741 attaaaaaaa aaaaaaaa
//
