LOCUS       NM_023991               2652 bp    mRNA    linear   ROD 07-APR-2024
DEFINITION  Rattus norvegicus protein kinase AMP-activated catalytic subunit
            alpha 2 (Prkaa2), mRNA.
ACCESSION   NM_023991
VERSION     NM_023991.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Yamagishi,H., Kirai,N., Morita,A., Kashihara,T. and Nakahara,T.
  TITLE     Role of monocarboxylate transporters in AMPK-mediated protection
            against excitotoxic injury in the rat retina
  JOURNAL   Eur J Pharmacol 970, 176510 (2024)
   PUBMED   38493917
  REMARK    GeneRIF: Role of monocarboxylate transporters in AMPK-mediated
            protection against excitotoxic injury in the rat retina.
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Zhu,Y., He,Y.J., Yu,Y., Xu,D., Yuan,S.Y. and Yan,H.
  TITLE     Aldehyde Dehydrogenase 2 Preserves Mitochondrial Function in the
            Ischemic Heart: A Redox-dependent Mechanism for AMPK Activation by
            Thioredoxin-1
  JOURNAL   J Cardiovasc Pharmacol 83 (1), 93-104 (2024)
   PUBMED   37816196
  REMARK    GeneRIF: Aldehyde Dehydrogenase 2 Preserves Mitochondrial Function
            in the Ischemic Heart: A Redox-dependent Mechanism for AMPK
            Activation by Thioredoxin-1.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2652)
  AUTHORS   Wang,B., Zhao,C., Wang,Y., Tian,X., Lin,J., Zhu,B., Zhou,Y.,
            Zhang,X., Li,N., Sun,Y., Xu,H. and Zhao,R.
  TITLE     Exercise ameliorating myocardial injury in type 2 diabetic rats by
            inhibiting excessive mitochondrial fission involving increased
            irisin expression and AMP-activated protein kinase phosphorylation
  JOURNAL   J Diabetes 16 (1), e13475 (2024)
   PUBMED   37721125
  REMARK    GeneRIF: Exercise ameliorating myocardial injury in type 2 diabetic
            rats by inhibiting excessive mitochondrial fission involving
            increased irisin expression and AMP-activated protein kinase
            phosphorylation.
REFERENCE   4  (bases 1 to 2652)
  AUTHORS   Zhao,A.R., Li,J., Wang,S.Q., Bian,L.H., Li,W.J. and Guo,J.Y.
  TITLE     Stress can affect mitochondrial energy metabolism and AMPK/SIRT1
            signaling pathway in rats
  JOURNAL   Brain Res Bull 203, 110770 (2023)
   PUBMED   37774988
  REMARK    GeneRIF: Stress can affect mitochondrial energy metabolism and
            AMPK/SIRT1 signaling pathway in rats.
REFERENCE   5  (bases 1 to 2652)
  AUTHORS   Zhan,H., Zhang,Q., Zhang,C., Cheng,J., Yang,Y., Liu,C., Li,S.,
            Wang,C., Yang,J., Ge,H., Zhou,D., Li,B., Wei,H. and Hu,C.
  TITLE     Targeted Activation of HNF4alpha by AMPK Inhibits Apoptosis and
            Ameliorates Neurological Injury Caused by Cardiac Arrest in Rats
  JOURNAL   Neurochem Res 48 (10), 3129-3145 (2023)
   PUBMED   37338793
  REMARK    GeneRIF: Targeted Activation of HNF4alpha by AMPK Inhibits
            Apoptosis and Ameliorates Neurological Injury Caused by Cardiac
            Arrest in Rats.
REFERENCE   6  (bases 1 to 2652)
  AUTHORS   Cheung,P.C., Salt,I.P., Davies,S.P., Hardie,D.G. and Carling,D.
  TITLE     Characterization of AMP-activated protein kinase gamma-subunit
            isoforms and their role in AMP binding
  JOURNAL   Biochem J 346 Pt 3 (Pt 3), 659-669 (2000)
   PUBMED   10698692
REFERENCE   7  (bases 1 to 2652)
  AUTHORS   Turnley,A.M., Stapleton,D., Mann,R.J., Witters,L.A., Kemp,B.E. and
            Bartlett,P.F.
  TITLE     Cellular distribution and developmental expression of AMP-activated
            protein kinase isoforms in mouse central nervous system
  JOURNAL   J Neurochem 72 (4), 1707-1716 (1999)
   PUBMED   10098881
REFERENCE   8  (bases 1 to 2652)
  AUTHORS   Woods,A., Salt,I., Scott,J., Hardie,D.G. and Carling,D.
  TITLE     The alpha1 and alpha2 isoforms of the AMP-activated protein kinase
            have similar activities in rat liver but exhibit differences in
            substrate specificity in vitro
  JOURNAL   FEBS Lett 397 (2-3), 347-351 (1996)
   PUBMED   8955377
REFERENCE   9  (bases 1 to 2652)
  AUTHORS   Gao,G., Widmer,J., Stapleton,D., Teh,T., Cox,T., Kemp,B.E. and
            Witters,L.A.
  TITLE     Catalytic subunits of the porcine and rat 5'-AMP-activated protein
            kinase are members of the SNF1 protein kinase family
  JOURNAL   Biochim Biophys Acta 1266 (1), 73-82 (1995)
   PUBMED   7718624
REFERENCE   10 (bases 1 to 2652)
  AUTHORS   Carling,D., Aguan,K., Woods,A., Verhoeven,A.J., Beri,R.K.,
            Brennan,C.H., Sidebottom,C., Davison,M.D. and Scott,J.
  TITLE     Mammalian AMP-activated protein kinase is homologous to yeast and
            plant protein kinases involved in the regulation of carbon
            metabolism
  JOURNAL   J Biol Chem 269 (15), 11442-11448 (1994)
   PUBMED   7908907
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from Z29486.1.
            
            [WARNING] On Apr 9, 2024 this sequence was replaced by NM_023991.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: Z29486.1, U12149.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132261,
                                           SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q34"
     gene            1..2652
                     /gene="Prkaa2"
                     /gene_synonym="Ampk; Ampka2"
                     /note="protein kinase AMP-activated catalytic subunit
                     alpha 2"
                     /db_xref="GeneID:78975"
                     /db_xref="RGD:620893"
     exon            1..101
                     /gene="Prkaa2"
                     /gene_synonym="Ampk; Ampka2"
                     /inference="alignment:Splign:2.1.0"
     CDS             8..1666
                     /gene="Prkaa2"
                     /gene_synonym="Ampk; Ampka2"
                     /EC_number="2.7.11.1"
                     /EC_number="2.7.11.31"
                     /note="AMPK alpha-2 chain; AMPK subunit alpha-2;
                     AMP-activated protein kinase; ACACA kinase; HMGCR kinase;
                     acetyl-CoA carboxylase kinase; protein kinase,
                     AMP-activated, alpha 2 catalytic subunit;
                     hydroxymethylglutaryl-CoA reductase kinase"
                     /codon_start=1
                     /product="5'-AMP-activated protein kinase catalytic
                     subunit alpha-2"
                     /protein_id="NP_076481.1"
                     /db_xref="GeneID:78975"
                     /db_xref="RGD:620893"
                     /translation="MAEKQKHDGRVKIGHYVLGDTLGVGTFGKVKIGEHQLTGHKVAV
                     KILNRQKIRSLDVVGKIKREIQNLKLFRHPHIIKLYQVISTPTDFFMVMEYVSGGELF
                     DYICKHGRVEEVEARRLFQQILSAVDYCHRHMVVHRDLKPENVLLDAQMNAKIADFGL
                     SNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSCGVILYALLCGTLPFDDEH
                     VPTLFKKIRGGVFYIPEYLNRSIATLLMHMLQVDPLKRATIKDIREHEWFKQDLPSYL
                     FPEDPSYDANVIDDEAVKEVCEKFECTESEVMNSLYSGDPQDQLAVAYHLIIDNRRIM
                     NQASEFYLASSPPTGSFMDDMAMHIPPGLKPHPERMPPLIADSPKARCPLDALNTTKP
                     KSLAVKKAKWHLGIRSQSKPYDIMAEVYRAMKQLDFEWKVVNAYHLRVRRKNPVTGNY
                     VKMSLQLYLVDNRSYLLDFKSIDDEVVEQRSGSSTPQRSCSAAGLHRPRSSVDSSTAE
                     NHSLSGSLTGSLTGSTLSSASPRLGSHTMDFFEMCASLITALAR"
     exon            102..243
                     /gene="Prkaa2"
                     /gene_synonym="Ampk; Ampka2"
                     /inference="alignment:Splign:2.1.0"
     exon            244..337
                     /gene="Prkaa2"
                     /gene_synonym="Ampk; Ampka2"
                     /inference="alignment:Splign:2.1.0"
     exon            338..482
                     /gene="Prkaa2"
                     /gene_synonym="Ampk; Ampka2"
                     /inference="alignment:Splign:2.1.0"
     exon            483..570
                     /gene="Prkaa2"
                     /gene_synonym="Ampk; Ampka2"
                     /inference="alignment:Splign:2.1.0"
     exon            571..795
                     /gene="Prkaa2"
                     /gene_synonym="Ampk; Ampka2"
                     /inference="alignment:Splign:2.1.0"
     exon            796..1300
                     /gene="Prkaa2"
                     /gene_synonym="Ampk; Ampka2"
                     /inference="alignment:Splign:2.1.0"
     exon            1301..1427
                     /gene="Prkaa2"
                     /gene_synonym="Ampk; Ampka2"
                     /inference="alignment:Splign:2.1.0"
     exon            1428..2652
                     /gene="Prkaa2"
                     /gene_synonym="Ampk; Ampka2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gccgaacatg gctgagaagc agaagcacga cgggcgtgtg aagatcggac actacgtgct
       61 gggggacacc ctgggcgtcg gcaccttcgg caaagtgaag attggagaac atcaattgac
      121 aggccataaa gtggcagtta agatcttaaa tagacagaag attcgcagtt tagatgttgt
      181 tggaaaaata aaacgagaaa ttcaaaatct taaactcttt cgtcatcctc atattatcaa
      241 actctaccaa gtgatcagca ctccaacaga cttttttatg gtaatggaat atgtgtctgg
      301 aggtgaattg ttcgactaca tctgtaaaca cgggagggtt gaagaggtgg aagctcgccg
      361 gctcttccag cagattctgt ctgccgtgga ctactgtcac aggcacatgg ttgtccacag
      421 ggacctgaag ccagagaacg tgttgctgga cgcccagatg aatgctaaga tagctgactt
      481 cggactctct aatatgatgt cagatggtga atttctacga actagctgtg gatcgccaaa
      541 ttatgcagca ccggaggtca tctcaggaag gctgtatgcg ggtcctgagg ttgatatctg
      601 gagctgtggt gttatcctgt atgcccttct ctgtggcacc ctcccgttcg acgatgagca
      661 cgtgcctacg ctctttaaga agatccgagg gggtgtgttc tacatcccgg agtatctcaa
      721 ccgttctatt gccactctgc tgatgcacat gctgcaggtg gaccccttga agcgagcaac
      781 tatcaaagac atacgagagc atgaatggtt taaacaggat ttgcccagtt acctctttcc
      841 tgaagacccc tcctatgatg ctaacgtcat tgatgatgag gctgtgaaag aagtatgtga
      901 aaaatttgag tgtacagaat cagaagtgat gaacagttta tacagtggtg accctcaaga
      961 ccagctcgca gtggcttatc atctcatcat tgacaatcgg agaataatga accaagccag
     1021 tgagttctac ctcgcctcca gtcctccaac gggttccttc atggacgata tggccatgca
     1081 cattcccccc ggcctgaaac cacatcctga aaggatgcca cctctcatag cagacagccc
     1141 caaagcacgc tgtccactgg atgcactcaa cacaactaag cccaaatctt tagctgtgaa
     1201 aaaagccaag tggcaccttg ggatccgaag ccagagcaaa ccatacgaca ttatggcgga
     1261 ggtgtaccga gctatgaagc agctggactt tgaatggaag gtagtgaatg cataccatct
     1321 tcgagtaaga agaaaaaacc cagtgactgg caattacgtg aaaatgagct tacagcttta
     1381 cctggttgac aatcggagct atcttctaga ctttaaaagc atcgatgatg aggtggtgga
     1441 gcagaggtct ggttcttcaa cacctcagcg ctcctgttct gctgccggcc tccacagacc
     1501 tcggtcaagt gtcgattcca gcacagccga gaaccattca ctgtctggct ctctcactgg
     1561 ttctttgact ggcagcactt tgtcctccgc ttccccgcgc ctgggcagtc ataccatgga
     1621 tttttttgaa atgtgcgcca gtcttatcac tgctttagcc cgttgataac ccaccaccgg
     1681 tctctgtctt tctgttaccg cactgtgaaa tcacatacac tcttcaaatt attaccgcac
     1741 tctcgggtac cacaggctct gcaatagaag ttatgtgaac attcccaggt gacatgcagt
     1801 gctgctggaa acacagaaat ctggccttct gtttactttt agaactctgt aactctgctg
     1861 tgcctatgat aggtatcaat agctaggaac ggctgagtgc tggtgaagct tgttaactta
     1921 cacccgtgaa ttcactacac atggtgagca cacctcactg atgaacccgc tgatctcggg
     1981 gtggttcggt gggaccgcct tccttcacgt ttagttcatg taaatcctgt ttgcctccta
     2041 aatttccata ggtgtcaggc tgtctaggca ctcttggaca agaagattca gaaatagagt
     2101 aactgtcagt gaaatattat ttaaatgtag aaatccgaaa atcctgtccc cttaaatatc
     2161 agaaaccaaa agtctttttt aatactttct gcaaatactg cctagtatta gccatacaga
     2221 ctgtgtttct gataatagga gcccagtctc ctagcttcct gacatttgtg caaggcccct
     2281 agttaaatcc cactaccaca aagcaatata aaaccatgca aagttagtgc atagttaaag
     2341 gaaacaggca gataccaaca gtctcttaaa aggaaatcta ttctttgatc tcatttgtgt
     2401 ttatgagact gggtagctgg gggcttgggg gcgggctggg agtcacagcc cttgggcatc
     2461 tttgctaatc cacttgaact ttgttattga tgccgagtcc tctctcttcc tcagtagcca
     2521 ctgtctttgc tcattgcttt tccctttttt aactccttta gatcaaaacc ttgctttggc
     2581 catagggtct ttaaatactt tcaaagcttg atctgctgtg accttcactg ttgaacctga
     2641 ttggacaggg aa
//
