LOCUS       NM_019142               4675 bp    mRNA    linear   ROD 17-FEB-2024
DEFINITION  Rattus norvegicus protein kinase AMP-activated catalytic subunit
            alpha 1 (Prkaa1), mRNA.
ACCESSION   NM_019142
VERSION     NM_019142.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Belo do Nascimento,I., Ates,G., Desmet,N., Beckers,P., Massie,A.
            and Hermans,E.
  TITLE     AMPKalpha1 Deficiency in Astrocytes from a Rat Model of ALS Is
            Associated with an Altered Metabolic Resilience
  JOURNAL   Biomolecules 13 (8), 1183 (2023)
   PUBMED   37627248
  REMARK    GeneRIF: AMPKalpha1 Deficiency in Astrocytes from a Rat Model of
            ALS Is Associated with an Altered Metabolic Resilience.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Zhang,Z., Chen,L., Chen,X., Qin,Y., Tian,C., Dai,X., Meng,R.,
            Zhong,Y., Liang,W., Shen,C., Zhang,J., Zhang,B. and Wei,X.
  TITLE     Exosomes derived from human umbilical cord mesenchymal stem cells
            (HUCMSC-EXO) regulate autophagy through AMPK-ULK1 signaling pathway
            to ameliorate diabetic cardiomyopathy
  JOURNAL   Biochem Biophys Res Commun 632, 195-203 (2022)
   PUBMED   36240643
  REMARK    GeneRIF: Exosomes derived from human umbilical cord mesenchymal
            stem cells (HUCMSC-EXO) regulate autophagy through AMPK-ULK1
            signaling pathway to ameliorate diabetic cardiomyopathy.
REFERENCE   3  (bases 1 to 4675)
  AUTHORS   Hermann,R., Mestre Cordero,V.E., Fernandez Pazos,M.L.M.,
            Cordoba,M.F., Reznik,F.J., Velez,D.E., Fellet,A.L. and Marina
            Prendes,M.G.
  TITLE     Role of AMPK in the protective effects exerted by triiodothyronine
            in ischemic-reperfused myocardium
  JOURNAL   J Mol Endocrinol 66 (3), 207-221 (2021)
   PUBMED   33640872
  REMARK    GeneRIF: Role of AMPK in the protective effects exerted by
            triiodothyronine in ischemic-reperfused myocardium.
REFERENCE   4  (bases 1 to 4675)
  AUTHORS   Qi,X. and Wang,J.
  TITLE     Melatonin improves mitochondrial biogenesis through the
            AMPK/PGC1alpha pathway to attenuate ischemia/reperfusion-induced
            myocardial damage
  JOURNAL   Aging (Albany NY) 12 (8), 7299-7312 (2020)
   PUBMED   32305957
  REMARK    GeneRIF: Melatonin improves mitochondrial biogenesis through the
            AMPK/PGC1alpha pathway to attenuate ischemia/reperfusion-induced
            myocardial damage.
REFERENCE   5  (bases 1 to 4675)
  AUTHORS   Woods,A., Salt,I., Scott,J., Hardie,D.G. and Carling,D.
  TITLE     The alpha1 and alpha2 isoforms of the AMP-activated protein kinase
            have similar activities in rat liver but exhibit differences in
            substrate specificity in vitro
  JOURNAL   FEBS Lett 397 (2-3), 347-351 (1996)
   PUBMED   8955377
REFERENCE   6  (bases 1 to 4675)
  AUTHORS   Stapleton,D., Mitchelhill,K.I., Gao,G., Widmer,J., Michell,B.J.,
            Teh,T., House,C.M., Fernandez,C.S., Cox,T., Witters,L.A. and
            Kemp,B.E.
  TITLE     Mammalian AMP-activated protein kinase subfamily
  JOURNAL   J Biol Chem 271 (2), 611-614 (1996)
   PUBMED   8557660
REFERENCE   7  (bases 1 to 4675)
  AUTHORS   Gao,G., Widmer,J., Stapleton,D., Teh,T., Cox,T., Kemp,B.E. and
            Witters,L.A.
  TITLE     Catalytic subunits of the porcine and rat 5'-AMP-activated protein
            kinase are members of the SNF1 protein kinase family
  JOURNAL   Biochim Biophys Acta 1266 (1), 73-82 (1995)
   PUBMED   7718624
REFERENCE   8  (bases 1 to 4675)
  AUTHORS   Stapleton,D., Gao,G., Michell,B.J., Widmer,J., Mitchelhill,K.,
            Teh,T., House,C.M., Witters,L.A. and Kemp,B.E.
  TITLE     Mammalian 5'-AMP-activated protein kinase non-catalytic subunits
            are homologs of proteins that interact with yeast Snf1 protein
            kinase
  JOURNAL   J Biol Chem 269 (47), 29343-29346 (1994)
   PUBMED   7961907
REFERENCE   9  (bases 1 to 4675)
  AUTHORS   Carling,D., Aguan,K., Woods,A., Verhoeven,A.J., Beri,R.K.,
            Brennan,C.H., Sidebottom,C., Davison,M.D. and Scott,J.
  TITLE     Mammalian AMP-activated protein kinase is homologous to yeast and
            plant protein kinases involved in the regulation of carbon
            metabolism
  JOURNAL   J Biol Chem 269 (15), 11442-11448 (1994)
   PUBMED   7908907
REFERENCE   10 (bases 1 to 4675)
  AUTHORS   Mitchelhill,K.I., Stapleton,D., Gao,G., House,C., Michell,B.,
            Katsis,F., Witters,L.A. and Kemp,B.E.
  TITLE     Mammalian AMP-activated protein kinase shares structural and
            functional homology with the catalytic domain of yeast Snf1 protein
            kinase
  JOURNAL   J Biol Chem 269 (4), 2361-2364 (1994)
   PUBMED   7905477
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000066.1.
            
            [WARNING] On Apr 3, 2024 this sequence was replaced by NM_019142.4.
            
            On Feb 10, 2021 this sequence version replaced NM_019142.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U40819.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-127               JACYVU010000066.1  1879654-1879780
            128-269             JACYVU010000066.1  1895482-1895623
            270-363             JACYVU010000066.1  1897467-1897560
            364-508             JACYVU010000066.1  1901441-1901585
            509-596             JACYVU010000066.1  1902626-1902713
            597-821             JACYVU010000066.1  1906580-1906804
            822-1308            JACYVU010000066.1  1910070-1910556
            1309-1435           JACYVU010000066.1  1910660-1910786
            1436-4675           JACYVU010000066.1  1912095-1915334
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q16"
     gene            1..4675
                     /gene="Prkaa1"
                     /gene_synonym="AMPKalpha1"
                     /note="protein kinase AMP-activated catalytic subunit
                     alpha 1"
                     /db_xref="GeneID:65248"
                     /db_xref="RGD:3387"
     CDS             1..1680
                     /gene="Prkaa1"
                     /gene_synonym="AMPKalpha1"
                     /EC_number="2.7.11.1"
                     /EC_number="2.7.11.26"
                     /EC_number="2.7.11.31"
                     /note="AMPK alpha-1 chain; ACACA kinase; HMGCR kinase;
                     acetyl-CoA carboxylase kinase; tau-protein kinase PRKAA1;
                     hydroxymethylglutaryl-CoA reductase kinase; protein
                     kinase, AMP-activated, alpha 1 catalytic subunit"
                     /codon_start=1
                     /product="5'-AMP-activated protein kinase catalytic
                     subunit alpha-1"
                     /protein_id="NP_062015.2"
                     /db_xref="GeneID:65248"
                     /db_xref="RGD:3387"
                     /translation="MRRLSSWRKMATAEKQKHDGRVKIGHYILGDTLGVGTFGKVKVG
                     KHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFM
                     VMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDA
                     HMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYAL
                     LCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREH
                     EWFKQDLPKYLFPEDPSYSSTMIDDEALKEVCEKFECSEEEVLSCLYNRNHQDPLAVA
                     YHLIIDNRRIMNEAKDFYLATSPPDSFLDDHHLTRPHPERVPFLVAETPRARHTLDEL
                     NPQKSKHQGVRKAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEWKVVNPYYLRVRRKNP
                     VTSTFSKMSLQLYQVDSRTYLLDFRSIDDEITEAKSGTATPQRSGSISNYRSCQRSDS
                     DAEAQGKPSEVSLTSSVTSLDSSPVDVAPRPGSHTIEFFEMCANLIKILAQ"
     exon            1..127
                     /gene="Prkaa1"
                     /gene_synonym="AMPKalpha1"
                     /inference="alignment:Splign:2.1.0"
     exon            128..269
                     /gene="Prkaa1"
                     /gene_synonym="AMPKalpha1"
                     /inference="alignment:Splign:2.1.0"
     exon            270..363
                     /gene="Prkaa1"
                     /gene_synonym="AMPKalpha1"
                     /inference="alignment:Splign:2.1.0"
     exon            364..508
                     /gene="Prkaa1"
                     /gene_synonym="AMPKalpha1"
                     /inference="alignment:Splign:2.1.0"
     exon            509..596
                     /gene="Prkaa1"
                     /gene_synonym="AMPKalpha1"
                     /inference="alignment:Splign:2.1.0"
     exon            597..821
                     /gene="Prkaa1"
                     /gene_synonym="AMPKalpha1"
                     /inference="alignment:Splign:2.1.0"
     exon            822..1308
                     /gene="Prkaa1"
                     /gene_synonym="AMPKalpha1"
                     /inference="alignment:Splign:2.1.0"
     exon            1309..1435
                     /gene="Prkaa1"
                     /gene_synonym="AMPKalpha1"
                     /inference="alignment:Splign:2.1.0"
     exon            1436..4675
                     /gene="Prkaa1"
                     /gene_synonym="AMPKalpha1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atgcgcagac tcagttcctg gagaaagatg gcgacggccg agaagcagaa gcacgacggg
       61 cgggtgaaga tcggccacta catcctgggg gacacgctgg gcgtcggcac cttcgggaaa
      121 gtgaaggtgg gcaagcacga gttgactgga cataaagttg ctgtgaagat actcaaccgg
      181 cagaagattc gaagcctgga cgtggtcggg aaaatccgca gagagatcca gaacctgaag
      241 cttttcaggc accctcatat aatcaaactg taccaggtca tcagtacacc gtctgatatt
      301 ttcatggtca tggaatatgt ctcaggagga gagctatttg attatatctg taaaaatgga
      361 aggttggacg aaaaggagag tcgacgtctg ttccagcaga tcctttctgg tgtggactat
      421 tgtcacaggc atatggtggt ccacagagat ttgaaacctg aaaacgtcct gcttgatgca
      481 cacatgaatg caaagatagc cgacttcggt ctttcaaaca tgatgtcaga tggtgaattt
      541 ttaagaacga gctgtggctc gcccaattat gctgcaccag aagtaatttc aggaagattg
      601 tacgcaggcc ctgaagtaga catctggagc agcggggtca ttctctatgc tttgctgtgt
      661 ggaactctcc cttttgatga tgaccacgtg ccaactcttt ttaagaagat atgtgacggg
      721 atattttata cccctcagta tttgaatccc tctgtaataa gccttttgaa gcatatgctg
      781 caggtagatc ctatgaagag ggccacaata aaagatatca gggaacatga atggtttaag
      841 caggaccttc caaaatatct ctttcctgaa gacccgtctt atagttcaac catgattgat
      901 gatgaagcct taaaagaagt gtgtgagaag ttcgagtgct cagaggagga ggtcctcagc
      961 tgcctgtaca acagaaacca ccaggaccca ctggcagttg cctaccacct cataatagac
     1021 aacaggagaa taatgaacga agccaaagat ttctacttgg caacaagccc acccgattct
     1081 ttcctcgatg atcaccattt aactcggcct caccctgaga gagtaccatt cttggttgcc
     1141 gaaacaccaa gggcccgaca caccctagat gaattaaacc cacagaaatc caaacaccaa
     1201 ggcgtacgga aggcaaagtg gcatttgggg attcgaagtc aaagccgacc caatgacatc
     1261 atggcagaag tgtgtagagc aatcaagcag ttggactatg aatggaaggt tgtaaacccc
     1321 tattatttgc gtgtgcgaag gaagaaccct gtgacaagca cattttccaa aatgagtcta
     1381 cagctatacc aagtggatag taggacttac ttattggatt tccgaagtat tgatgatgag
     1441 attacagaag ccaaatcagg gactgctact ccacagagat cgggatccat cagcaactat
     1501 cgatcttgcc aaaggagcga ctccgacgcc gaggctcaag gaaagccctc agaagtctct
     1561 cttacctcat ccgtgacctc cctcgactcc tctcctgttg acgtagctcc aagaccagga
     1621 agtcacacga tagaattttt tgaaatgtgt gcaaatctaa ttaaaattct tgcacagtaa
     1681 acctaaaact ttgcttattt ctttgatgca ataagcatgc ttactaaata atagccaaat
     1741 acttccactt accgagttat atgtgtgtgt atatataact aaggactggc cttctgcaat
     1801 gcagcttgca cagggcctgg aaaatgatcg attgtttaag cctaatgtgc agaaaggaat
     1861 caggatcatt ctgttagccc ttattcagta ggcacacatc gcatagtaac acacagtgga
     1921 ctataggcct caggctcact cgatttttgg ccatttgaat ttgctgtaac aggtctctgt
     1981 tgaccataaa tttaccataa agcctttaca tatcagtaca tgttgatgtg ttttatatta
     2041 gcttcatgaa tttattgagt gagtttgcct agaatcccca cagaccttcc tagatgatac
     2101 aagacctagg cgatgctgtt ttctgggctc cttaactttc gaaatagtta ctgtttccca
     2161 ttttgggtaa cattatgaac tatttccata ttcctcttaa aatactattt ttatgtagca
     2221 agtcatttca gataacagtc taatttaaaa tttttctttg gcttgtaagc ccccaaccca
     2281 ccagaattta tatgttcaga ccctccgagc cctgaacttg cttttacagt ttccccctta
     2341 aacgttttgt gtatgaagtg ctgctttgga tccaagagcc gagttgctca cactgtaccg
     2401 aatcatgttg cttctgtcag tgcacagaca tgctatcatg tcagccagta ttgaatgtca
     2461 tttgattgtg tattttttta agtcaatcca gcgcatagaa cggacattgt ttaaaagctc
     2521 tgaatgctgg gattgggttt ttaacccctg attttattct gaacaatttc taaagaaaga
     2581 aaagttgaaa gaatacagtg actgcatgta tatctactac taggctccta caaccactct
     2641 ttggccatat ttgctctcta gagccacacg tgtatgaaat acagatatgc acatatatat
     2701 gagtgtatgt ttgattttat tattaacctt tgagatgtta agatacatat accctaaata
     2761 cttcaacatc ccctaaaaat agtgataatg tccttttaaa aaaaatctcc tgaatccatt
     2821 ataaaatcaa taatttccag atagtgcctg gtctccagat ttaactgtaa ctgcccagaa
     2881 tcccattaaa gttacagcct gatttcattt gtagttcttt gatcaggtta ataacactct
     2941 tttgaaaaga tgtagaagaa atcctttctt caaaatggtc aaacttattc caggttttaa
     3001 ttaagaaaag tgagtggcta aagaagtttg atctttcttc aatctctgac ttttatgcct
     3061 cctctttcag tgatagcata gcgtaatagg tgtaagtgga gatttaaagt cacaaaagtc
     3121 agctggtatt gtatgagcta ataattcatg tcatattgac tgtatttcgt ttcttttaag
     3181 tttaagaaat tggtcagagg ctccagctca ttggaaaggc tgagtgaaag gcattgtgaa
     3241 aataggtttc ctttaaacat taatcactga cacccccccc ccccccgcca tgtaagagag
     3301 acccctcccc cactgcattc attttaaaat acgttgcccc aatctcaaat ctgtttagca
     3361 cttatgtaaa cagatgggaa caaatttgga ggacatgata gctgcataaa aggttacgct
     3421 acagtttatg cactgatttc tggacaaaaa aaatggcgct tctttttgtt tggagcttgt
     3481 tttattctga ttcgaatgct gtataaagtg atggagactc cgtttacatg agttcccaac
     3541 tgtattcata cctgtgtatg tgccctctgt gtggggctca cagatgtcac ttatgctgtc
     3601 tgagtgcagt gctgtgttcc caatgtgtag aacagaagac tgcattttta aagttacaga
     3661 tttgtaaata gaactaagta atatctctat aaactgtttg gggggacggg gcacaaaagg
     3721 aggagccaat taaataggac cttctaaaat ttgttaattt tgtaaacttt gcttctttta
     3781 taagttattc tgtgattcct tttagttact gagttttatt ttgaagatat ttaaatattg
     3841 cacttgtata aaaagtacga taaatgcaga ctgttgcagt caaaattttt tttaaagcta
     3901 ggatatctga aatgacaact ttcagttcag tatgtcaagc caggacagaa ttttcacaaa
     3961 tgtattgtag tttgcaaatt cttatactga aaatgtaagg ggagtcaatg caaatgattt
     4021 ttaatctttt tttttttatc ttttcagcaa tttatatttt ttttgtgatt ttatgcaatc
     4081 atatttttac ttcgtcttga caactgaaag ctgtatgatg tttttgccag aaaagtactg
     4141 tatacatagt tttaaatata acagattttg ctgatatgta aaaattttgc cattaaagtt
     4201 gatttctcat gaaaggaact tttctatcaa gttgggagac acgagagctt aatatatcta
     4261 atttaaaata atttcaccga aataaacacc attgctttta ctttgattat attttttctt
     4321 aagatgcttt tgtagtgttt tggagtttta gataatttta tataaattct ctccctgcct
     4381 tgcaccatcc tttggatatt tgataccatt cacctatgaa ctaaagtggc tgggtgtgta
     4441 agatctgtat tccaagtggc atcttgggaa agaaaaccat gttgtctaaa ttcttcttaa
     4501 ctcctcccct ccaccgttca ttgtgggctc tgacatgaat gaaattccac acacagccta
     4561 actgtaagtc ggttgttggg aggagtcaat cattgtggtc tatgtatgtt tgcccagagt
     4621 aacctgattt agaaagatgg cataataaag aaaacattca ttttttcatc tgtta
//
