LOCUS       NM_001108721            3445 bp    mRNA    linear   ROD 11-DEC-2024
DEFINITION  Rattus norvegicus proline rich membrane anchor 1 (Prima1), mRNA.
ACCESSION   NM_001108721 XM_001066450 XM_001073629
VERSION     NM_001108721.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Xie,H.Q., Liang,D., Leung,K.W., Chen,V.P., Zhu,K.Y., Chan,W.K.,
            Choi,R.C., Massoulie,J. and Tsim,K.W.
  TITLE     Targeting acetylcholinesterase to membrane rafts: a function
            mediated by the proline-rich membrane anchor (PRiMA) in neurons
  JOURNAL   J Biol Chem 285 (15), 11537-11546 (2010)
   PUBMED   20147288
  REMARK    GeneRIF: PRiMA in neurons has a role in targeting
            acetylcholinesterase to membrane rafts
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Tsim,K.W., Leung,K.W., Mok,K.W., Chen,V.P., Zhu,K.Y., Zhu,J.T.,
            Guo,A.J., Bi,C.W., Zheng,K.Y., Lau,D.T., Xie,H.Q. and Choi,R.C.
  TITLE     Expression and Localization of PRiMA-linked globular form
            acetylcholinesterase in vertebrate neuromuscular junctions
  JOURNAL   J Mol Neurosci 40 (1-2), 40-46 (2010)
   PUBMED   19680821
  REMARK    GeneRIF: These findings suggest that the restricted localization of
            PRiMA-linked G4 AChE at the nmjs could be contributed by the
            pre-synaptic motor neuron and/or the post-synaptic muscle fiber.
REFERENCE   3  (bases 1 to 3445)
  AUTHORS   Leung,K.W., Xie,H.Q., Chen,V.P., Mok,M.K., Chu,G.K., Choi,R.C. and
            Tsim,K.W.
  TITLE     Restricted localization of proline-rich membrane anchor (PRiMA) of
            globular form acetylcholinesterase at the neuromuscular
            junctions--contribution and expression from motor neurons
  JOURNAL   FEBS J 276 (11), 3031-3042 (2009)
   PUBMED   19490106
  REMARK    GeneRIF: Data show that after denervation, the expression of PRiMA,
            AChE(T) and G(4) AChE decreased markedly in the spinal cord, and in
            fast- and slow-twitch muscles.
REFERENCE   4  (bases 1 to 3445)
  AUTHORS   Xie,H.Q., Choi,R.C., Leung,K.W., Chen,V.P., Chu,G.K. and Tsim,K.W.
  TITLE     Transcriptional regulation of proline-rich membrane anchor (PRiMA)
            of globular form acetylcholinesterase in neuron: an inductive
            effect of neuron differentiation
  JOURNAL   Brain Res 1265, 13-23 (2009)
   PUBMED   19368807
  REMARK    GeneRIF: These results suggested that a MAP kinase signaling
            pathway served as one of the transcriptional regulators in
            controlling PRiMA gene expression during the neuronal
            differentiation process.
REFERENCE   5  (bases 1 to 3445)
  AUTHORS   Noureddine,H., Carvalho,S., Schmitt,C., Massoulie,J. and Bon,S.
  TITLE     Acetylcholinesterase associates differently with its anchoring
            proteins ColQ and PRiMA
  JOURNAL   J Biol Chem 283 (30), 20722-20732 (2008)
   PUBMED   18511416
  REMARK    GeneRIF: Acetylcholinesterase associates differently with its
            anchoring proteins ColQ and PRiMA
REFERENCE   6  (bases 1 to 3445)
  AUTHORS   Florea,L., Di Francesco,V., Miller,J., Turner,R., Yao,A.,
            Harris,M., Walenz,B., Mobarry,C., Merkulov,G.V., Charlab,R.,
            Dew,I., Deng,Z., Istrail,S., Li,P. and Sutton,G.
  TITLE     Gene and alternative splicing annotation with AIR
  JOURNAL   Genome Res 15 (1), 54-66 (2005)
   PUBMED   15632090
REFERENCE   7  (bases 1 to 3445)
  AUTHORS   Perrier,A.L., Massoulie,J. and Krejci,E.
  TITLE     PRiMA: the membrane anchor of acetylcholinesterase in the brain
  JOURNAL   Neuron 33 (2), 275-285 (2002)
   PUBMED   11804574
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000006.1.
            
            On or before Sep 25, 2008 this sequence version replaced
            XM_001073629.1, XM_001066450.1, NM_001108721.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360194.2407620.1,
                                           SRR26360176.1188233.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760393, SAMEA5760433
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-72                JAXUCZ010000006.1  128154565-128154636 c
            73-195              JAXUCZ010000006.1  128153833-128153955 c
            196-331             JAXUCZ010000006.1  128148254-128148389 c
            332-461             JAXUCZ010000006.1  128111020-128111149 c
            462-3445            JAXUCZ010000006.1  128103175-128106158 c
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q32"
     gene            1..3445
                     /gene="Prima1"
                     /gene_synonym="PRiMA; Prima1l"
                     /note="proline rich membrane anchor 1"
                     /db_xref="GeneID:690195"
                     /db_xref="RGD:1583978"
     exon            1..72
                     /gene="Prima1"
                     /gene_synonym="PRiMA; Prima1l"
                     /inference="alignment:Splign:2.1.0"
     exon            73..195
                     /gene="Prima1"
                     /gene_synonym="PRiMA; Prima1l"
                     /inference="alignment:Splign:2.1.0"
     CDS             103..564
                     /gene="Prima1"
                     /gene_synonym="PRiMA; Prima1l"
                     /codon_start=1
                     /product="proline-rich membrane anchor 1 precursor"
                     /protein_id="NP_001102191.2"
                     /db_xref="GeneID:690195"
                     /db_xref="RGD:1583978"
                     /translation="MLLRDLVLRHGCCWPSLLLHCALHPLWGLVQVTHAEPQKSCSKV
                     TDSCQHICQCRPPPPLPPPPPPPPPPRLLSAPAPNSTSCPAEDSWWSGLVIIVAVVCA
                     SLVFLTVLVIICYKAIKRKPLRKDENGASVAEYPMSSSPSNKGVDVNAAVV"
     exon            196..331
                     /gene="Prima1"
                     /gene_synonym="PRiMA; Prima1l"
                     /inference="alignment:Splign:2.1.0"
     exon            332..461
                     /gene="Prima1"
                     /gene_synonym="PRiMA; Prima1l"
                     /inference="alignment:Splign:2.1.0"
     exon            462..3445
                     /gene="Prima1"
                     /gene_synonym="PRiMA; Prima1l"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agagctgccg tggggggaac cctgagctcc cttctccgtg tcgacgatcg gtgggggtcg
       61 ccaccaggca agttagctca ccagtgggcg ccgcgggccg agatgcttct ccgggacctg
      121 gtgctgcgcc atggctgctg ctggccctca cttctgctgc attgtgcgct gcacccactc
      181 tggggtttag ttcaggtgac gcacgctgag ccccagaagt cttgctctaa ggtgactgac
      241 agctgccaac acatctgcca gtgccggccc ccacctcctc tgcccccgcc acccccacct
      301 ccgccacctc ccagactact ctctgcccca gctcccaact ctacctcctg ccccgcggag
      361 gacagctggt ggtctggtct ggtaatcatc gtcgcggtgg tctgtgcctc tctggtgttt
      421 ctgactgtcc tggtcatcat ttgctacaaa gccataaaga ggaaaccact gagaaaagat
      481 gaaaatggcg ccagcgtagc tgagtacccc atgagctcct cgccgagtaa caaaggggtg
      541 gatgtgaacg ctgcggtggt gtgaacctgc aggccctggg acccactggc cagagaggca
      601 ccatggtgac attggtagac atggatgaca ggtggacatg agctgaccct gggcctgtcc
      661 aggactctgt gctttgtagg cctcgagcct cctgaaagag atagcatcct cttcccaggc
      721 tagccctggt cactcctgag gggtgcggca ccgacaccag gcctgagcca ggctcccctg
      781 catgtggccc ctgagcagca ccaccaccca gggctctcca tactcctcct tgtctcctgt
      841 tgggcacact gactgtccac cagttactac tgaaagcatg tattccagtg aaagcagagg
      901 gtctgtccat ctgcctgggg cagagaggta ccccacgtta gccagaagaa aggtgagcca
      961 gggacttctc ccattctgga agcttcctct cctgcaggaa acccacctgc tcctctgtgg
     1021 acattcttac aaaagccatc atcacacagc catgagacta atgtcccctt ccaaaggtgc
     1081 ttcccacaca tctgtccatc actgtatctg ccatggtctt ccgtctccag agcctgtgtc
     1141 accctccttc acccaaccac agcctagcct gtgactcctt actgcaagcc tgcctctgga
     1201 agccgtgagt tcagcctaca tagtccccca gggagtgaac tcagtgtctt ccagatgccc
     1261 aggcagaggg tgtctcaaga aagtcagaag ttccctttgc ccactctgac acccacccct
     1321 gcctcttcag gaagcatccg tgtgaaccaa accctgcctc accggacaga ggtcacaact
     1381 tgtgccaaaa ccttacaagc gctctgtgcc aggctgatgc tggtctagtg ctctgtgacc
     1441 cgcttactcc cagcagtccc ctgaagccta aatttggtat cagaaagttc ccaggagaag
     1501 gtggccttct gtgtgtccct gaggtggctt tctctgtggg ccagaactct taaggcacca
     1561 gggcttcctg aatgtgggta gaagagaaag tggagaagag tcgtctagcc ttgataaccc
     1621 tccacccata ggcttgtttc cctggaggcc acccacacag tgaggcccat cccttggcct
     1681 ttgtgatttt gtatcccttg cctcctctcc cccaactctc ctcaccagcc acatgtactt
     1741 gcccagaagg aagcagacct actggagagt tcaacgctcc cacagtgaag agatttagct
     1801 gggtacatta gtcccctcct cagccaccag agatctctgg tgggtgtttg tgctacatga
     1861 gaatttcaaa gccaggtaaa actgacagac atgaggatca gtcctttgta gctgggtgtc
     1921 ctgaagttgc cactgctctg ctcccctgtc aataggtgta ggttaagggg cgttccaggg
     1981 cacggcatag ctcctgtaaa tgacatttgc cttcctttgc ttgaggacat ggttttgggt
     2041 tagttttgaa ttttcctttt acagcctgcc ttgtgctccg gcacggggtt gccaagatcc
     2101 ttctctgggg ctgccccacc tcacccaggg gagcattggg ccatcctctc tggatgggat
     2161 cccaaatgat gacgaaacct cagaggaggt agatcaggga caggcacgac aagggtgaga
     2221 agcctctcag gctccagccc agtgccttgt ctctcacgcc atcacacctt gatctaaact
     2281 gagacaaggg ccaccagtct cctggtgccc gtgcccacag cagacactgg gcagatagga
     2341 ggcctggccc tagttgcaaa catacacacc ttggaacctc acaggggata gtaataccct
     2401 cctattccca cctccagtta tcaagtccca ggagtgagga aggaatcctc ctagctggag
     2461 aggagctcct tactaggggg cctgagggct gggtagagtc tgagatctcc atgtacctat
     2521 gtccagacct tggcttaaac ctaggtagca cacggtgttt gtgtatatgt gtgtgctatg
     2581 agccagcagg gactatgact gaggttgcac tgtgcttggc tagtgtacat gtaaattctc
     2641 tttagtgggc ttgcattacc ccaccccgca gttaggggga gaccaaggga agggcctagg
     2701 ttttaaaagg gttccccgga gaatggcctc tcaagctcaa gcttggccac ttagagggcg
     2761 tgtctcacat cccaatagaa ggcagtagct tttaagccca catggctgta acatacacaa
     2821 agtcaatgtc aaccaagagc ctgaacttag accttcactt caaagggccc tgagtcagct
     2881 ccacgaagac ctgttctcag cgcagcctga atgaatgcag aggtcagaag aaatgcccgt
     2941 gttcacgagt ttcctccgac gtttttcact gtggttaagt catgtcaaaa ctcaacagcg
     3001 tttaggctct cccagttacc aggtgtttct acaagccttc tagaagacat tttggatgaa
     3061 agagaagtag atgtaaaaaa aaatatatag gcgtattata tatagttata tatatatata
     3121 aagagatacc tatctataca aatatatata tatatataaa tattgtactc tgatctgtac
     3181 aaatatgacc taaacagtcc aggtagtatg tgacccgcag tgcatgaatg taaataccgt
     3241 gaactctgcc ttcttagcaa tgcgtgcgtg tgtgtgtgcg tgtgtgtgcg tgtgcgtgtg
     3301 tgtgtatgtg tgtgtgtggc gactgttgcc gctggcacat ggccttggtg ggcaggtaca
     3361 cgcgtcaaga ctcagcagcc tcttccttac gaaaaaggag gcatcgaggt ttttttttta
     3421 ctgtcaataa aaatgtactt ataaa
//
