LOCUS       NM_001399183            2024 bp    mRNA    linear   ROD 29-MAR-2024
DEFINITION  Rattus norvegicus DNA primase subunit 2 (Prim2), transcript variant
            1, mRNA.
ACCESSION   NM_001399183 XM_006244686
VERSION     NM_001399183.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Popanda,O., Flohr,C., Dai,J.C., Hunzicker,A. and Thielmann,H.W.
  TITLE     A mutation in subunit B of the DNA polymerase alpha-primase complex
            from Novikoff hepatoma cells concomitant with a conformational
            change and abnormal catalytic properties of the DNA polymerase
            alpha-primase complex
  JOURNAL   Mol Carcinog 31 (4), 171-183 (2001)
   PUBMED   11536367
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000009.1.
            
            On Jan 4, 2022 this sequence version replaced XM_006244686.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643295.34830.1,
                                           SRR26643294.35862.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-146               JAXUCZ010000009.1  43391333-43391478   c
            147-309             JAXUCZ010000009.1  43390481-43390643   c
            310-413             JAXUCZ010000009.1  43388733-43388836   c
            414-493             JAXUCZ010000009.1  43385580-43385659   c
            494-614             JAXUCZ010000009.1  43384067-43384187   c
            615-710             JAXUCZ010000009.1  43353152-43353247   c
            711-848             JAXUCZ010000009.1  43351185-43351322   c
            849-916             JAXUCZ010000009.1  43273243-43273310   c
            917-989             JAXUCZ010000009.1  43247348-43247420   c
            990-1175            JAXUCZ010000009.1  43245284-43245469   c
            1176-1302           JAXUCZ010000009.1  43216029-43216155   c
            1303-1385           JAXUCZ010000009.1  43212013-43212095   c
            1386-1454           JAXUCZ010000009.1  43199242-43199310   c
            1455-2024           JAXUCZ010000009.1  43188350-43188919   c
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="9"
                     /map="9q21"
     gene            1..2024
                     /gene="Prim2"
                     /gene_synonym="Pola3"
                     /note="DNA primase subunit 2"
                     /db_xref="GeneID:301323"
                     /db_xref="RGD:631433"
     exon            1..146
                     /gene="Prim2"
                     /gene_synonym="Pola3"
                     /inference="alignment:Splign:2.1.0"
     exon            147..309
                     /gene="Prim2"
                     /gene_synonym="Pola3"
                     /inference="alignment:Splign:2.1.0"
     CDS             156..1679
                     /gene="Prim2"
                     /gene_synonym="Pola3"
                     /note="isoform 1 is encoded by transcript variant 1; DNA
                     primase large subunit; p58; DNA primase 58 kDa subunit;
                     DNA primase, p58 subunit; DNA polymerase alpha subunit III
                     (primase); primase, DNA, polypeptide 2; primase (DNA)
                     subunit 2"
                     /codon_start=1
                     /product="DNA primase large subunit isoform 1"
                     /protein_id="NP_001386112.1"
                     /db_xref="GeneID:301323"
                     /db_xref="RGD:631433"
                     /translation="MQFSGRTRKKLRLAGDQRNACYPHSLQFYLQPPTENISLTEFES
                     LAFDRVKLLKAIENLGVSYVKGTEQYQSKLEAEIRKLKFSYRENLEDEYEPRRRDHIS
                     HFILRLAYCQSEDLRRWFIQQEMDLLRFRFSILPKDKVQSFLKDTHLHFEAISDEEKT
                     LREQDIMASSPSLSGVRWESESVYKVPFADALDLFRGRKVYLEDGFAYVPLKDIVAII
                     LNEFRATLSKALALTARSLPAVQSDERLQPLLSHLSHSYTGQDYSTQKSTGKISLDQI
                     DSLSTKSFPPCMRQLHKALRENHHLRHGGRMQYGLFLKGIGLTLEQALQFWKQEFIKG
                     KMDPDKFDKGYSYNIRHSFGKEGKRTDYTPFSCMKIILTNPPSQGDFHGCPFRHSDAE
                     LLKQKMQTYKIPASGISQILDLVKGNHYQVACQKYFEMTHNVDDCGFSLNHPNQFFFE
                     SQRILTGGKDIKKEASHPETPQHKPSTQKTKDATSALASLDSSLEMDLEGLEDYFSK"
     exon            310..413
                     /gene="Prim2"
                     /gene_synonym="Pola3"
                     /inference="alignment:Splign:2.1.0"
     exon            414..493
                     /gene="Prim2"
                     /gene_synonym="Pola3"
                     /inference="alignment:Splign:2.1.0"
     exon            494..614
                     /gene="Prim2"
                     /gene_synonym="Pola3"
                     /inference="alignment:Splign:2.1.0"
     exon            615..710
                     /gene="Prim2"
                     /gene_synonym="Pola3"
                     /inference="alignment:Splign:2.1.0"
     exon            711..848
                     /gene="Prim2"
                     /gene_synonym="Pola3"
                     /inference="alignment:Splign:2.1.0"
     exon            849..916
                     /gene="Prim2"
                     /gene_synonym="Pola3"
                     /inference="alignment:Splign:2.1.0"
     exon            917..989
                     /gene="Prim2"
                     /gene_synonym="Pola3"
                     /inference="alignment:Splign:2.1.0"
     exon            990..1175
                     /gene="Prim2"
                     /gene_synonym="Pola3"
                     /inference="alignment:Splign:2.1.0"
     exon            1176..1302
                     /gene="Prim2"
                     /gene_synonym="Pola3"
                     /inference="alignment:Splign:2.1.0"
     exon            1303..1385
                     /gene="Prim2"
                     /gene_synonym="Pola3"
                     /inference="alignment:Splign:2.1.0"
     exon            1386..1454
                     /gene="Prim2"
                     /gene_synonym="Pola3"
                     /inference="alignment:Splign:2.1.0"
     exon            1455..2024
                     /gene="Prim2"
                     /gene_synonym="Pola3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccaaatgaac tctcccgcca cagggaaaga gcggctgtca gcgcgggtgt ttcgtccagt
       61 ttggttgctg gctggttaaa gaatatcctg cgggtttgtt tggagcggag agaaactgtg
      121 gggttggaag cttgcgagtt gacttggtgg tcaagatgca gttctcagga aggacccgga
      181 agaagctgag attggcaggt gaccagagaa acgcttgtta ccctcacagc cttcagttct
      241 atctgcagcc acctactgaa aacatatcat tgacagagtt tgaaagcttg gcttttgata
      301 gagtaaaatt gcttaaagca attgagaatc ttggtgtgag ctatgtgaaa ggaaccgaac
      361 agtaccagag taaactggag gctgagattc gaaagctcaa gttttcgtac agggagaacc
      421 tggaggatga gtacgagcct cggaggaggg accacatctc ccacttcatc ctgcgcctcg
      481 cttactgcca gtcggaagat cttagacggt ggtttattca acaggagatg gatctgcttc
      541 ggttccgatt cagtatttta cccaaggata aagtccagag tttcttgaag gatactcact
      601 tgcattttga ggctatcagt gatgaggaga agacccttcg ggaacaggat atcatggcgt
      661 cctctcccag cctaagtggg gtcaggtggg aatcggagtc agtgtataag gtcccctttg
      721 ctgacgctct ggacctgttc agaggaagga aagtctactt ggaagacggc tttgcttatg
      781 tgccacttaa ggacattgtg gccattatcc tgaacgagtt tagagccacg ctgtctaagg
      841 ccttggcact aacagccagg tccctgcctg ctgtgcagtc cgatgaacga cttcagcctc
      901 tgctcagcca cctcagtcat tcttacaccg gccaagatta tagtacccag aagagcaccg
      961 ggaagatttc cttagatcag attgattcgc tttcaacaaa atccttccca ccttgcatgc
     1021 gtcagctgca caaggcgctg agggaaaacc accatcttcg tcatggaggc cggatgcagt
     1081 atggcctgtt cctcaagggc attgggctaa cgttggagca agcattgcag ttctggaagc
     1141 aagagtttat caaaggaaag atggacccag acaagtttga taaaggttac tcttacaata
     1201 tccgacatag ctttggaaag gaaggcaaga ggacagacta tacgccattc agttgcatga
     1261 agattatcct gaccaaccca ccaagccagg gggatttcca tgggtgccca ttccgtcaca
     1321 gtgatgcaga gctgctgaag cagaagatgc agacctacaa gatccctgcc tcggggatca
     1381 gccagatttt ggatttggta aaggggaatc attaccaggt ggcctgtcag aagtacttcg
     1441 agatgacgca caatgtggac gattgtggct tttctttgaa tcatccgaat cagttctttt
     1501 ttgagagcca gcgaatccta actggtggca aagatatcaa gaaggaagca agccacccag
     1561 aaacgcctca gcacaaaccc agcacccaga agaccaagga tgccacgtct gctctggcct
     1621 ctctagattc ctccctggaa atggatctgg aggggctaga agactacttt agtaaatgac
     1681 gtggcccctg gagcaactgg agcaaataca ttcctgtttg aaaggtttgc ctttgtaaac
     1741 ctttctcttc ctacccacgc atgctcacac atgtactggg aaagcaggct tgcttccacc
     1801 ttacttttga cagaatgaaa aaggaagagt tcctatggaa acctttcatt gaatatttcg
     1861 ttttgaatcc tgttccagaa aatttaaaac agtactcagg gactaaaatc ctcaagcagc
     1921 aatacaacta aaactaactt ttaaagtttc acctagtttt ggccaagtct tcttttggat
     1981 cgattttgct aataaacaac atgtatatga ctatatccga gtaa
//
