LOCUS       NM_001304365            1628 bp    mRNA    linear   ROD 23-MAR-2023
DEFINITION  Rattus norvegicus DNA primase subunit 1 (Prim1), transcript variant
            1, mRNA.
ACCESSION   NM_001304365
VERSION     NM_001304365.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ghosh D, Lippert D, Krokhin O, Cortens JP and Wilkins JA.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Popanda O, Flohr C, Dai JC, Hunzicker A and Thielmann HW.
  TITLE     A mutation in subunit B of the DNA polymerase alpha-primase complex
            from Novikoff hepatoma cells concomitant with a conformational
            change and abnormal catalytic properties of the DNA polymerase
            alpha-primase complex
  JOURNAL   Mol Carcinog 31 (4), 171-183 (2001)
   PUBMED   11536367
REFERENCE   3  (bases 1 to 1628)
  AUTHORS   Copeland WC.
  TITLE     Expression, purification, and characterization of the two human
            primase subunits and truncated complexes from Escherichia coli
  JOURNAL   Protein Expr Purif 9 (1), 1-9 (1997)
   PUBMED   9116489
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000171.1.
            
            [WARNING] On Apr 3, 2024 this sequence was replaced by
            NM_001304365.3.
            
            On Mar 5, 2021 this sequence version replaced NM_001304365.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from genomic sequence
            data because no single transcript from the reference strain was
            available for the full length of the gene. The extent of this
            transcript is supported by transcript alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMD00132261,
                              SAMD00132262 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-151               JACYVU010000171.1  446373-446523
            152-309             JACYVU010000171.1  447127-447284
            310-416             JACYVU010000171.1  449427-449533
            417-490             JACYVU010000171.1  449598-449671
            491-627             JACYVU010000171.1  451470-451606
            628-686             JACYVU010000171.1  453050-453108
            687-796             JACYVU010000171.1  453655-453764
            797-888             JACYVU010000171.1  456665-456756
            889-1027            JACYVU010000171.1  456882-457020
            1028-1097           JACYVU010000171.1  458725-458794
            1098-1183           JACYVU010000171.1  459275-459360
            1184-1282           JACYVU010000171.1  461578-461676
            1283-1628           JACYVU010000171.1  462171-462516
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q11"
     gene            1..1628
                     /gene="Prim1"
                     /note="DNA primase subunit 1"
                     /db_xref="GeneID:246327"
                     /db_xref="RGD:621380"
     exon            1..151
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     CDS             49..1302
                     /gene="Prim1"
                     /EC_number="2.7.7.7"
                     /note="isoform 1 is encoded by transcript variant 1; DNA
                     primase small subunit, 49kDa; DNA primase, p49 subunit;
                     primase, DNA, polypeptide 1; primase (DNA) subunit 1"
                     /codon_start=1
                     /product="DNA primase small subunit isoform 1"
                     /protein_id="NP_001291294.2"
                     /db_xref="GeneID:246327"
                     /db_xref="RGD:621380"
                     /translation="MELFDPAELPELLKLYYRRLFPYAQYYRWLNYGGVTKNYFQHRE
                     FSFTLKDDIYIRYQSFNNQSDLEKEMQKMNPYKIDIGAVYSHRPSQHNTVKLGAFQAQ
                     EKELVFDIDMTDYDDVRRCCSSADICSKCWTLMTMAMRIIDRALKEDFGFKHRLWVYS
                     GRRGVHCWVCDESVRKLSSAVRSGIVEYLSLIKGGHDVKKKVHLNEKVHPFVRKSINI
                     IKKYFEEYALVDQDILENKENWDKILALVPETVHDELQKGFQRFHSSPQRWEYLRKVA
                     SAPQNTKNDKCGPWLEWEIMLQYCFPRLDINVSKGVNHLLKSPFSVHPKTGRISVPID
                     FQKVDQFDPFVVPTISAICRELDVVSTTEKEKEENETDSRHRVRDYKRTSLAPYVKVF
                     EQFLENLDKSRKGALLKKSDLQKDF"
     exon            152..309
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            310..416
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            417..490
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            491..627
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            628..686
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            687..796
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            797..888
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            889..1027
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            1028..1097
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            1098..1183
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            1184..1282
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            1283..1628
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tcccgccaat tctcgtgtca gtcctgtctg ggtctagttc cttacttcat ggagctattt
       61 gatcccgcgg agctgccgga gctactcaag ctttattacc gaagactctt tccttacgcc
      121 cagtactatc gttggctcaa ctatggcgga gtgacaaaga attactttca acaccgtgaa
      181 ttctcattca cactgaaaga tgatatttac attcgctacc agtccttcaa caaccagagt
      241 gatctggaaa aggagatgca gaaaatgaat ccatataaga ttgatatagg tgcagtatat
      301 tctcacagac ccagccaaca caacacagtg aagctggggg ctttccaggc tcaggagaaa
      361 gaactggtct ttgacattga catgacagac tatgacgacg taaggagatg ctgcagttcc
      421 gcagacatat gctccaagtg ctggactctc atgacaatgg ccatgcgcat catagatcgg
      481 gcattgaagg aggactttgg atttaagcac cgcctctggg tgtattcagg aaggagaggt
      541 gtacattgtt gggtctgtga tgagtctgtt cgaaagctgt cctctgctgt tcgctccggg
      601 atcgtggagt acttgagtct tataaagggt ggtcacgatg ttaaaaagaa ggttcaccta
      661 aatgagaaag ttcacccttt tgtcagaaaa tctataaaca taattaaaaa gtactttgaa
      721 gaatatgcct tagttgacca agatattctt gaaaataaag aaaactggga taagatttta
      781 gcccttgttc ctgagacagt tcatgatgag ctgcaaaaag ggtttcagag atttcacagt
      841 tcacctcagc gctgggaata cctgaggaaa gtggccagtg caccacagaa taccaagaat
      901 gataagtgtg gcccctggct agaatgggag attatgctcc agtactgttt cccacgcctg
      961 gatatcaacg tcagcaaagg agtcaaccat ttactgaaga gcccttttag tgttcaccct
     1021 aagacaggtc gcatttctgt gcctatcgat tttcagaaag tggatcagtt tgatccattt
     1081 gttgttccaa ctataagtgc tatctgccgg gaattggatg tggtttccac cactgagaag
     1141 gagaaggagg aaaatgaaac tgacagcaga cacagagtca gagattataa aaggaccagc
     1201 ctagcaccat acgtgaaagt atttgaacag tttctcgaaa acctggataa atctcggaaa
     1261 ggggcacttc tcaagaagag cgatttacaa aaagatttct gaagaaaaca accccatcaa
     1321 accaatgtga atgccttcta cattcaacca tggagcattt agtcaatgca gtagaatcgt
     1381 cagtgtgact gaatccttaa agcagagctt tctttctttt ctttcttttt tctttttttc
     1441 ggagctgggg acgaacccag ggccttgagc ttgttaggca agcgctctac cactgagcta
     1501 aatccccaac ccctcagagc tttcttttga gaaatcattt atgtcaaaga gacctggttt
     1561 ctgagaccaa agagggtgtc aagggccctg aaagaaacaa taaatcaagg aataaatgca
     1621 ctgcaaaa
//
