LOCUS       NM_001008768            1624 bp    mRNA    linear   ROD 12-JAN-2022
DEFINITION  Rattus norvegicus DNA primase subunit 1 (Prim1), transcript variant
            2, mRNA.
ACCESSION   NM_001008768
VERSION     NM_001008768.3
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ghosh D, Lippert D, Krokhin O, Cortens JP and Wilkins JA.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Popanda O, Flohr C, Dai JC, Hunzicker A and Thielmann HW.
  TITLE     A mutation in subunit B of the DNA polymerase alpha-primase complex
            from Novikoff hepatoma cells concomitant with a conformational
            change and abnormal catalytic properties of the DNA polymerase
            alpha-primase complex
  JOURNAL   Mol Carcinog 31 (4), 171-183 (2001)
   PUBMED   11536367
REFERENCE   3  (bases 1 to 1624)
  AUTHORS   Copeland WC.
  TITLE     Expression, purification, and characterization of the two human
            primase subunits and truncated complexes from Escherichia coli
  JOURNAL   Protein Expr Purif 9 (1), 1-9 (1997)
   PUBMED   9116489
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000171.1.
            
            [WARNING] On Apr 3, 2024 this sequence was replaced by
            NM_001008768.4.
            
            On Feb 10, 2021 this sequence version replaced NM_001008768.2.
            
            Transcript Variant: This variant (2) uses an alternate splice site
            in the 5' coding region, which results in a frameshift, compared to
            variant 1. It encodes isoform 2, which has a shorter and distinct
            N-terminus, compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from genomic sequence
            data because no single transcript from the reference strain was
            available for the full length of the gene. The extent of this
            transcript is supported by transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC088272.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132262, SAMD00132263
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-151               JACYVU010000171.1  446373-446523
            152-309             JACYVU010000171.1  447127-447284
            310-412             JACYVU010000171.1  449427-449529
            413-486             JACYVU010000171.1  449598-449671
            487-623             JACYVU010000171.1  451470-451606
            624-682             JACYVU010000171.1  453050-453108
            683-792             JACYVU010000171.1  453655-453764
            793-884             JACYVU010000171.1  456665-456756
            885-1023            JACYVU010000171.1  456882-457020
            1024-1093           JACYVU010000171.1  458725-458794
            1094-1179           JACYVU010000171.1  459275-459360
            1180-1278           JACYVU010000171.1  461578-461676
            1279-1624           JACYVU010000171.1  462171-462516
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q11"
     gene            1..1624
                     /gene="Prim1"
                     /note="DNA primase subunit 1"
                     /db_xref="GeneID:246327"
                     /db_xref="RGD:621380"
     exon            1..151
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            152..309
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            310..412
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     CDS             408..1298
                     /gene="Prim1"
                     /EC_number="2.7.7.7"
                     /note="isoform 2 is encoded by transcript variant 2; DNA
                     primase small subunit, 49kDa; DNA primase, p49 subunit;
                     primase, DNA, polypeptide 1; primase (DNA) subunit 1"
                     /codon_start=1
                     /product="DNA primase small subunit isoform 2"
                     /protein_id="NP_001008768.2"
                     /db_xref="GeneID:246327"
                     /db_xref="RGD:621380"
                     /translation="MLSADICSKCWTLMTMAMRIIDRALKEDFGFKHRLWVYSGRRGV
                     HCWVCDESVRKLSSAVRSGIVEYLSLIKGGHDVKKKVHLNEKVHPFVRKSINIIKKYF
                     EEYALVDQDILENKENWDKILALVPETVHDELQKGFQRFHSSPQRWEYLRKVASAPQN
                     TKNDKCGPWLEWEIMLQYCFPRLDINVSKGVNHLLKSPFSVHPKTGRISVPIDFQKVD
                     QFDPFVVPTISAICRELDVVSTTEKEKEENETDSRHRVRDYKRTSLAPYVKVFEQFLE
                     NLDKSRKGALLKKSDLQKDF"
     exon            413..486
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            487..623
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            624..682
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            683..792
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            793..884
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            885..1023
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            1024..1093
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            1094..1179
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            1180..1278
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
     exon            1279..1624
                     /gene="Prim1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tcccgccaat tctcgtgtca gtcctgtctg ggtctagttc cttacttcat ggagctattt
       61 gatcccgcgg agctgccgga gctactcaag ctttattacc gaagactctt tccttacgcc
      121 cagtactatc gttggctcaa ctatggcgga gtgacaaaga attactttca acaccgtgaa
      181 ttctcattca cactgaaaga tgatatttac attcgctacc agtccttcaa caaccagagt
      241 gatctggaaa aggagatgca gaaaatgaat ccatataaga ttgatatagg tgcagtatat
      301 tctcacagac ccagccaaca caacacagtg aagctggggg ctttccaggc tcaggagaaa
      361 gaactggtct ttgacattga catgacagac tatgacgacg taaggagatg ctttccgcag
      421 acatatgctc caagtgctgg actctcatga caatggccat gcgcatcata gatcgggcat
      481 tgaaggagga ctttggattt aagcaccgcc tctgggtgta ttcaggaagg agaggtgtac
      541 attgttgggt ctgtgatgag tctgttcgaa agctgtcctc tgctgttcgc tccgggatcg
      601 tggagtactt gagtcttata aagggtggtc acgatgttaa aaagaaggtt cacctaaatg
      661 agaaagttca cccttttgtc agaaaatcta taaacataat taaaaagtac tttgaagaat
      721 atgccttagt tgaccaagat attcttgaaa ataaagaaaa ctgggataag attttagccc
      781 ttgttcctga gacagttcat gatgagctgc aaaaagggtt tcagagattt cacagttcac
      841 ctcagcgctg ggaatacctg aggaaagtgg ccagtgcacc acagaatacc aagaatgata
      901 agtgtggccc ctggctagaa tgggagatta tgctccagta ctgtttccca cgcctggata
      961 tcaacgtcag caaaggagtc aaccatttac tgaagagccc ttttagtgtt caccctaaga
     1021 caggtcgcat ttctgtgcct atcgattttc agaaagtgga tcagtttgat ccatttgttg
     1081 ttccaactat aagtgctatc tgccgggaat tggatgtggt ttccaccact gagaaggaga
     1141 aggaggaaaa tgaaactgac agcagacaca gagtcagaga ttataaaagg accagcctag
     1201 caccatacgt gaaagtattt gaacagtttc tcgaaaacct ggataaatct cggaaagggg
     1261 cacttctcaa gaagagcgat ttacaaaaag atttctgaag aaaacaaccc catcaaacca
     1321 atgtgaatgc cttctacatt caaccatgga gcatttagtc aatgcagtag aatcgtcagt
     1381 gtgactgaat ccttaaagca gagctttctt tcttttcttt cttttttctt tttttcggag
     1441 ctggggacga acccagggcc ttgagcttgt taggcaagcg ctctaccact gagctaaatc
     1501 cccaacccct cagagctttc ttttgagaaa tcatttatgt caaagagacc tggtttctga
     1561 gaccaaagag ggtgtcaagg gccctgaaag aaacaataaa tcaaggaata aatgcactgc
     1621 aaaa
//
