LOCUS       NM_001393795           11021 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus
            phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange
            factor 2 (Prex2), mRNA.
ACCESSION   NM_001393795 XM_006237753
VERSION     NM_001393795.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 11021)
  AUTHORS   Feng,F., Wu,X., Shi,X., Gao,Q., Wu,Y., Yu,Y., Cheng,Q., Li,B.,
            Yi,B., Liu,C., Hao,Q., Zhang,L., Gao,C. and Jiang,X.
  TITLE     Comprehensive analysis of genomic alterations of Chinese hilar
            cholangiocarcinoma patients
  JOURNAL   Int J Clin Oncol 26 (4), 717-727 (2021)
   PUBMED   33387086
REFERENCE   2  (bases 1 to 11021)
  AUTHORS   Liu,L., Zeng,P., Yang,S. and Yuan,Z.
  TITLE     Leveraging methylation to identify the potential causal genes
            associated with survival in lung adenocarcinoma and lung squamous
            cell carcinoma
  JOURNAL   Oncol Lett 20 (1), 193-200 (2020)
   PUBMED   32537022
REFERENCE   3  (bases 1 to 11021)
  AUTHORS   Liao,Z., Zheng,Q., Wei,T., Zhang,Y., Ma,J., Zhao,Z., Sun,H. and
            Nan,K.
  TITLE     MicroRNA-561 Affects Proliferation and Cell Cycle Transition
            Through PTEN/AKT Signaling Pathway by Targeting P-REX2a in NSCLC
  JOURNAL   Oncol Res 28 (2), 147-159 (2020)
   PUBMED   31711559
REFERENCE   4  (bases 1 to 11021)
  AUTHORS   Yu,J., Ou,Z., Lei,Y., Chen,L., Su,Q. and Zhang,K.
  TITLE     LncRNA MYCNOS facilitates proliferation and invasion in
            hepatocellular carcinoma by regulating miR-340
  JOURNAL   Hum Cell 33 (1), 148-158 (2020)
   PUBMED   31776854
REFERENCE   5  (bases 1 to 11021)
  AUTHORS   Li,C.H., Yen,C.H., Chen,Y.F., Lee,K.J., Fang,C.C., Zhang,X.,
            Lai,C.C., Huang,S.F., Lin,H.K. and Arthur Chen,Y.M.
  TITLE     Characterization of the GNMT-HectH9-PREX2 tripartite relationship
            in the pathogenesis of hepatocellular carcinoma
  JOURNAL   Int J Cancer 140 (10), 2284-2297 (2017)
   PUBMED   28205209
REFERENCE   6  (bases 1 to 11021)
  AUTHORS   Liao,Z., Wang,X., Zeng,Y. and Zou,Q.
  TITLE     Identification of DEP domain-containing proteins by a machine
            learning method and experimental analysis of their expression in
            human HCC tissues
  JOURNAL   Sci Rep 6, 39655 (2016)
   PUBMED   28000796
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 11021)
  AUTHORS   Lan,X., Xiao,F., Ding,Q., Liu,J., Liu,J., Li,J., Zhang,J. and
            Tian,D.A.
  TITLE     The effect of CXCL9 on the invasion ability of hepatocellular
            carcinoma through up-regulation of PREX2
  JOURNAL   J Mol Histol 45 (6), 689-696 (2014)
   PUBMED   25151370
REFERENCE   8  (bases 1 to 11021)
  AUTHORS   Hodakoski,C., Hopkins,B.D., Barrows,D., Mense,S.M., Keniry,M.,
            Anderson,K.E., Kern,P.A., Hawkins,P.T., Stephens,L.R. and
            Parsons,R.
  TITLE     Regulation of PTEN inhibition by the pleckstrin homology domain of
            P-REX2 during insulin signaling and glucose homeostasis
  JOURNAL   Proc Natl Acad Sci U S A 111 (1), 155-160 (2014)
   PUBMED   24367090
REFERENCE   9  (bases 1 to 11021)
  AUTHORS   Donald,S., Humby,T., Fyfe,I., Segonds-Pichon,A., Walker,S.A.,
            Andrews,S.R., Coadwell,W.J., Emson,P., Wilkinson,L.S. and
            Welch,H.C.
  TITLE     P-Rex2 regulates Purkinje cell dendrite morphology and motor
            coordination
  JOURNAL   Proc Natl Acad Sci U S A 105 (11), 4483-4488 (2008)
   PUBMED   18334636
REFERENCE   10 (bases 1 to 11021)
  AUTHORS   Donald,S., Hill,K., Lecureuil,C., Barnouin,R., Krugmann,S., John
            Coadwell,W., Andrews,S.R., Walker,S.A., Hawkins,P.T., Stephens,L.R.
            and Welch,H.C.
  TITLE     P-Rex2, a new guanine-nucleotide exchange factor for Rac
  JOURNAL   FEBS Lett 572 (1-3), 172-176 (2004)
   PUBMED   15304343
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000005.1.
            
            On Mar 11, 2021 this sequence version replaced XM_006237753.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643298.26122.1,
                                           SRR26643289.14634.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760386,
                                           SAMEA5760393 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-573               JAXUCZ010000005.1  13035505-13036077   c
            574-645             JAXUCZ010000005.1  12971239-12971310   c
            646-768             JAXUCZ010000005.1  12969651-12969773   c
            769-873             JAXUCZ010000005.1  12967443-12967547   c
            874-975             JAXUCZ010000005.1  12960313-12960414   c
            976-1137            JAXUCZ010000005.1  12949837-12949998   c
            1138-1271           JAXUCZ010000005.1  12940515-12940648   c
            1272-1375           JAXUCZ010000005.1  12935203-12935306   c
            1376-1525           JAXUCZ010000005.1  12928331-12928480   c
            1526-1670           JAXUCZ010000005.1  12928000-12928144   c
            1671-1771           JAXUCZ010000005.1  12925546-12925646   c
            1772-1875           JAXUCZ010000005.1  12911956-12912059   c
            1876-1925           JAXUCZ010000005.1  12911308-12911357   c
            1926-2001           JAXUCZ010000005.1  12908520-12908595   c
            2002-2074           JAXUCZ010000005.1  12905134-12905206   c
            2075-2217           JAXUCZ010000005.1  12901887-12902029   c
            2218-2310           JAXUCZ010000005.1  12901656-12901748   c
            2311-2459           JAXUCZ010000005.1  12899252-12899400   c
            2460-2545           JAXUCZ010000005.1  12892640-12892725   c
            2546-2682           JAXUCZ010000005.1  12888790-12888926   c
            2683-2800           JAXUCZ010000005.1  12886208-12886325   c
            2801-2985           JAXUCZ010000005.1  12875513-12875697   c
            2986-3147           JAXUCZ010000005.1  12872228-12872389   c
            3148-3370           JAXUCZ010000005.1  12859087-12859309   c
            3371-3578           JAXUCZ010000005.1  12858388-12858595   c
            3579-3758           JAXUCZ010000005.1  12846070-12846249   c
            3759-3853           JAXUCZ010000005.1  12843926-12844020   c
            3854-3936           JAXUCZ010000005.1  12843234-12843316   c
            3937-4027           JAXUCZ010000005.1  12842500-12842590   c
            4028-4156           JAXUCZ010000005.1  12841557-12841685   c
            4157-4198           JAXUCZ010000005.1  12837350-12837391   c
            4199-4416           JAXUCZ010000005.1  12830098-12830315   c
            4417-4519           JAXUCZ010000005.1  12826457-12826559   c
            4520-4663           JAXUCZ010000005.1  12820210-12820353   c
            4664-4778           JAXUCZ010000005.1  12800063-12800177   c
            4779-4845           JAXUCZ010000005.1  12758648-12758714   c
            4846-5036           JAXUCZ010000005.1  12758272-12758462   c
            5037-5139           JAXUCZ010000005.1  12739316-12739418   c
            5140-5207           JAXUCZ010000005.1  12733806-12733873   c
            5208-11021          JAXUCZ010000005.1  12720683-12726496   c
FEATURES             Location/Qualifiers
     source          1..11021
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q11"
     gene            1..11021
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /note="phosphatidylinositol-3,4,5-trisphosphate-dependent
                     Rac exchange factor 2"
                     /db_xref="GeneID:312912"
                     /db_xref="RGD:1307865"
     exon            1..573
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     CDS             457..5253
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /note="DEP domain containing 2"
                     /codon_start=1
                     /product="phosphatidylinositol
                     3,4,5-trisphosphate-dependent Rac exchanger 2 protein"
                     /protein_id="NP_001380724.1"
                     /db_xref="GeneID:312912"
                     /db_xref="RGD:1307865"
                     /translation="MSDESARDADKQLRLRVCVLSELQKTERDYVGTLEFLVSAFLHR
                     MNQCAAAKLDKNVTEETVKMLFSNIEEILAVHKEFLKVVEECLHPEPNAQQEVGTCFL
                     NFKDKFRIYDEYCSNHEKAQKLLLELNKIRTIRTFLLNCMLLGGRKNTDVPLEGYLVT
                     PIQRICKYPLLLKELLKRTPRKHSDYTAVMEALQAMKAVCSNINEAKRQMEKLEVLEE
                     WQAHIEGWEGSNITDTCTEMLMCGVLMKISSGNIQERVFFLFDNLLVYCKRKHRRLKN
                     SKASTDGHRYVFRGRINTEVMEVENVDDGTADFHSSGHIVVNGWKIHNTAKNKWFVCM
                     AKSPEEKHEWFEAILKERERRKGLKLGMEQDTWVMISEQGEKLYKMMCKQGNLIKDRK
                     RKLTTFPKCFLGSEFVSWLLEIGEIHRPEEGVHLGQALLENGIIHHVTDKHQFKPEQM
                     LYRFRYDDGTFYPRSEMQDVISKGVRLYCRLHSLFTPVVRDKDYHLRTYKSVVMANKL
                     IDWLIAQGDCRTREEAMIFAVGLCDNGFMHHVLEKSEFKDEPLLFRFFADEEMEGSNM
                     KHRLMKHDLKVVENVIAKSLLIKSNEGSYGFGLEDKNKVPIIKLVEKGSNAEMAGMEV
                     GKKIFAINGDLVFLRPFPEVDCFLKSCLNSRKPLRVLVSTKPRETVKIPDSADGLGFQ
                     IRGFGPSVVHAVGRGTVAAAAGLHPGQCIIKVNGINVSKETHASVIAHVTACRKYKRP
                     MKQDSIQWVYDSLESAQEDIQKSHSKPPGDEAGDVFDCKVEDVMDKFNTLAIIDGKKE
                     HVSLTVDNVHLEYGVVYEYDSTAGTKCNVVEKMVEPKGFFSLTAKILEALAKSDEHFV
                     QNCTSLNSLNEVIATDLQSKFTSMCSERVEHVCHRISSYGRFSRVLKNRAWPTFKQAK
                     AKMSPLHSSDFCPTNCHVNVMEVSYPKASASLGSAFGVQLDSRKHNSHDKENRSADPG
                     KLSPMVYIQHTITTMAAPSGLSLGHKDGHGLRYLLKEEDLETQDIYHKLLGKLQTALK
                     EVEMCVCQIDDLLSSITYSPKLERKTTEYVIPTDSDNEKGERNSKRVCFNVAGDEQED
                     SGHDTVSNRDSYSDCNSNRNSIASFTSICSSQCSSYFHSDEMDSGDELPTSIRISHDK
                     QDKIHTCLEQLFIQLDSITNLLKGRAVVRAFEQTKYLTPGRGLQEFQQEMEAKLSCPR
                     RLRLHLKQDPWNLPSGVRALAQSFRKYVEEVKCRILLALLEYSDSETQLRRDMVFCQS
                     LVATVCAFSEQLMAALNQMFDNSKENEMETWEASRRWLDQIANAGVLFHFQSLLSPNL
                     KDEQAMLEDTLVALFDLEKVSFFFKPSEEEPLVANVPLTYQVEGSRQALKVVFYMDSY
                     HFEQLPQRLKNGGGFKIHPVLFSQALESMEGYCYRDNISVEEFQAQINTASLEKVKQY
                     NQKLRAFYLDKSNSPPNATSKAAYIEKLMRPLNALDELYRLVTSFIRSKRIAACVNTP
                     CSASGVGLLSVSSELCDRLGACHIIMCSSGVHRCTLSVTLEQAITLARSHGLPPRYIM
                     QATDVMRKQGARVQNTAKNLGVRDRTPQSAPRLYKLCEPPPPVGDE"
     exon            574..645
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            646..768
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            769..873
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            874..975
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1137
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..1271
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1272..1375
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1376..1525
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1526..1670
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1671..1771
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1772..1875
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1876..1925
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1926..2001
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            2002..2074
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            2075..2217
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            2218..2310
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            2311..2459
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            2460..2545
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            2546..2682
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            2683..2800
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            2801..2985
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            2986..3147
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            3148..3370
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            3371..3578
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            3579..3758
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            3759..3853
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            3854..3936
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            3937..4027
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            4028..4156
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            4157..4198
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            4199..4416
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            4417..4519
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            4520..4663
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            4664..4778
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            4779..4845
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            4846..5036
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            5037..5139
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            5140..5207
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
     exon            5208..11021
                     /gene="Prex2"
                     /gene_synonym="Depdc2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gagtgagtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt ttagttagct
       61 ggcacgatac acttccccag ggttgtgttt cctttttcct gagatttggc taagggtaaa
      121 gggtcggagt cacatggatt gaaagcattt gattgatgtc caagtcaccg ctgtggctgg
      181 agccgcctcc cctcaggctg gggccgcgtg aggccggagc agagggccgg gccgggctcc
      241 cgctctctgg ggactgtcct gctctgagtc ccgcgcgtcc cgcgcgcgcg ccacagccgg
      301 agcacagtcc tctgcccacg acccagcctc gggtcagcat gtgaagctgc gtgttccggc
      361 tcggactccg caaaggcggc aactttgcgg gcggcagtgg tggcagcagg cgcggggcag
      421 cgcacttgcc cttagccccg cgccccgccg gccaccatga gcgacgagag cgccagggac
      481 gcggacaagc agcttcgcct gcgcgtgtgc gtgctcagcg agctacagaa gaccgagcgc
      541 gactacgtgg gcacgctgga gttcctggtg tcggccttct tgcatcgaat gaaccagtgt
      601 gcagcagcta agctcgataa aaatgtgacg gaggaaacag tgaagatgtt attctccaat
      661 atcgaggaga tcctcgccgt acataaggag ttcttgaaag ttgtagaaga atgcttacat
      721 ccggaaccca acgcgcagca ggaagtggga acctgcttcc ttaactttaa agacaagttt
      781 cgtatctatg atgagtattg cagcaaccac gaaaaggcac aaaaattgct tcttgagctg
      841 aacaaaataa gaacaatccg gacgtttctt ttgaactgca tgctgcttgg aggacggaag
      901 aatacagatg tgcccctgga aggatattta gtgacaccga tacaaaggat ttgcaagtac
      961 cctctccttt tgaaggaatt actgaagcgg accccacgga aacacagtga ctacacagca
     1021 gtgatggaag ccctccaagc catgaaagct gtttgttcca acattaatga ggccaagaga
     1081 cagatggaga aactggaagt tttagaagag tggcaggcac acattgaagg ctgggagggt
     1141 tccaacatta ccgacacctg tactgagatg ctcatgtgtg gagtcctaat gaaaatttct
     1201 tccggaaata ttcaagaaag ggtgtttttt ctttttgata atcttttggt atactgcaaa
     1261 agaaaacaca gacggctgaa gaatagcaag gcatccacgg atgggcatcg gtatgtcttc
     1321 cgagggcgga tcaataccga ggtgatggaa gtggagaatg tggatgacgg tacagctgat
     1381 tttcacagca gtgggcacat agttgtgaat gggtggaaga tacacaacac agcgaaaaat
     1441 aaatggttcg tatgcatggc taaaagccct gaagaaaagc atgaatggtt tgaagctatc
     1501 ttgaaagaaa gagaacgtcg gaaaggttta aaattaggaa tggagcaaga tacctgggta
     1561 atgatctccg agcagggtga gaaattgtat aaaatgatgt gcaagcaggg aaatctgatc
     1621 aaagacagaa aaagaaaact gaccacattc cccaagtgct tcctgggaag tgaatttgtc
     1681 tcatggctgc tggaaattgg agaaatccac aggcctgaag agggtgttca cttgggacaa
     1741 gcattgctag agaacgggat catccaccat gttaccgata agcaccagtt caagccagag
     1801 caaatgctat accgattccg atatgatgat ggtacctttt accccaggag tgagatgcag
     1861 gatgtgattt ctaagggtgt aagactctac tgtcgtctcc atagtctgtt tactccggtg
     1921 gtcagagata aagattatca tttaagaact tacaaatctg tcgtcatggc caacaaattg
     1981 atagactggt taattgcaca gggtgattgc cgtaccaggg aagaggcaat gatatttgct
     2041 gttgggcttt gtgacaatgg attcatgcat cacgtcctag aaaaaagtga attcaaagac
     2101 gagcctctac ttttccgctt ttttgccgat gaggaaatgg aaggatcaaa tatgaaacac
     2161 aggctgatga aacatgactt gaaggtggtg gagaatgtca tagctaagtc actgttgatt
     2221 aaatccaatg aaggcagcta tggatttggg ctggaagaca aaaataaagt tccaataata
     2281 aagttagtgg aaaaagggtc gaatgctgag atggctggca tggaagttgg gaagaagata
     2341 tttgctatta atggtgacct ggtttttctg agaccttttc ctgaagttga ttgcttcctg
     2401 aaatcgtgct tgaacagcag aaagccactg agagtccttg tgagcacaaa gccaagggag
     2461 accgttaaga tcccagattc tgcagatggg cttggtttcc agatcagagg ctttggtcct
     2521 tcagttgtac atgctgtggg aagaggtact gtggctgcag cagctggcct tcatccagga
     2581 cagtgtatta tcaaagtaaa tggaatcaac gtcagcaagg agacacatgc cagtgtcatt
     2641 gcacatgtca ccgcctgccg caaatacaag cggcccatga agcaagattc catacagtgg
     2701 gtctatgata gtcttgaaag tgctcaggaa gacattcaga agtcccactc aaagccccct
     2761 ggagacgagg ctggggatgt ttttgactgc aaagtagaag atgtcatgga caagttcaac
     2821 accttggcca ttatcgacgg caagaaggag catgtgagtc tgacggtgga caatgtccac
     2881 ctcgagtacg gggttgtgta cgagtatgac agcacggctg gcacaaagtg caatgtggtg
     2941 gagaagatgg tggagcccaa agggttcttc agcctaactg ccaagattct ggaagcccta
     3001 gcaaaaagtg atgaacattt tgtccaaaac tgcaccagcc tcaattcttt gaatgaagtg
     3061 atcgccactg accttcagag taaattcacc agcatgtgca gtgagagagt cgagcatgtg
     3121 tgccacagaa tatccagcta tgggaggttc tctcgggtgc tgaagaacag agcctggccc
     3181 acctttaagc aggccaaagc taagatgtcc ccgctgcaca gcagtgactt ctgccctacc
     3241 aactgccatg tgaatgtgat ggaagtttct tatccgaaag catcggcttc cctgggcagc
     3301 gccttcggtg tgcagctcga tagtaggaaa cacaattccc atgataagga aaataggtct
     3361 gcagacccag ggaaactgag ccccatggta tacattcaac acacaatcac aaccatggca
     3421 gccccttcag gcctgtcgct gggacacaag gatggccatg gcctccggta tttgctgaaa
     3481 gaagaagact tagaaactca agacatctat cacaaactcc tgggcaagct gcagactgca
     3541 ctgaaggagg tggagatgtg cgtttgtcaa atcgatgacc ttctgtcttc aataacatat
     3601 tctcctaaat tggagcgtaa gacaacagag tatgtgatac caacggacag cgacaatgaa
     3661 aagggagaga gaaatagcaa acgtgtctgt tttaatgtag caggagatga acaagaggat
     3721 tcaggtcatg atacagtcag caacagagac tcttacagtg actgcaacag caacaggaat
     3781 tccattgcct ccttcacgag tatctgcagc agccagtgca gttcctactt tcacagtgat
     3841 gagatggact caggtgatga gcttccaacc agcatccgga tatctcatga taaacaggac
     3901 aagatccaca cttgcctgga gcagctcttc atccagttgg attcaataac caacctcttg
     3961 aaaggacgag ctgttgtaag ggcctttgag caaaccaagt atctcacacc aggtcgagga
     4021 ttacaagaat tccaacagga aatggaggca aagctgagtt gccccaggag gttgcggctc
     4081 cacctcaagc aggatccttg gaatcttccc agcggtgtcc gggctcttgc acagagcttc
     4141 agaaaatatg ttgaagaggt gaagtgccgc atacttctgg ctctccttga atactcagat
     4201 agtgaaaccc agctccggag agacatggtt ttctgccaaa gtcttgtggc cactgtctgt
     4261 gccttctctg agcagctcat ggctgcctta aaccagatgt ttgataatag caaggaaaat
     4321 gaaatggaga cttgggaagc cagcaggagg tggctggacc agattgcaaa tgcgggtgtg
     4381 ctttttcact tccagtcact tctgtcacca aatttgaaag atgagcaagc catgcttgag
     4441 gatacactgg ttgccctgtt tgatttggaa aaagtttcct ttttcttcaa accatcagaa
     4501 gaggaaccac ttgttgcaaa tgtgcctctt acataccaag tggaaggaag ccggcaagct
     4561 ctgaaagttg tcttctacat ggatagttac cattttgagc agctgcccca acggctgaag
     4621 aatggaggcg ggtttaaaat tcaccctgtt ctcttttcac aagcactgga gagcatggaa
     4681 ggatattgct atagagacaa catctccgtg gaagagtttc aagcgcagat aaacacagcc
     4741 tccctggaaa aggtcaaaca gtacaatcag aagctcaggg cattctactt ggacaagtca
     4801 aattccccac caaatgccac atctaaagct gcctacatag agaagctcat gaggcctctc
     4861 aatgctttag atgagcttta ccggctggtc acctcgttta tcagatccaa gcgcatcgct
     4921 gcctgtgtga acacaccttg cagcgcctct ggagtcggac tgctgtcagt ttcttcggag
     4981 ctgtgcgaca ggctcggtgc ctgtcacatc atcatgtgca gcagtggtgt gcaccggtgt
     5041 accctgagtg tgacactgga gcaagccatc actctagcaa gaagccacgg gctccctcct
     5101 aggtacatta tgcaggccac agatgtgatg agaaagcagg gtgcaagagt tcagaacaca
     5161 gctaagaact tgggggttag agaccggact cctcagtcag ctccgaggtt gtataagctg
     5221 tgtgagccac cacctccagt gggagacgaa taagcattcc caggagccac gagacagatg
     5281 cttccaaatg ctggccaagt caacattctc ccctgaagac aaagaattgc atatttaaga
     5341 agcaaagttt tcccatggtg aaaataactg atgtttggtt tggatattta tactggaact
     5401 agaagttcct taagacagtt cagcctcaca ttctagatat attaaaatta cagattcata
     5461 ttagcataaa attcactgct caaatttgta gaaaaatata cagagataaa agatttaact
     5521 attttgattc ctttctcatt cgttaattat attgtatttg tttttagctt ccccagtggg
     5581 cagatataat attggtagtt tctcgtgaag aagtctgtca ctaaaaatga ttgccacact
     5641 ctggaaacag tcatacaaaa atgttaagaa cagtgtagat ggggagttac aggtgtcccg
     5701 tctcccactg gtgccaccta gaagtgcaca tgtgggaggt aggaacatac acacctccct
     5761 ttcttctaag gcttcctggt aatggagaaa gctttgaggt cctggtaaga cttttcttac
     5821 tcttgtccta tgtactccct ggccatgaaa acactccagc aggcaggtgc ttttcttgat
     5881 ctcttcagtg agccacgaat cctgggtgat gtaccattga tggctgtgta tagggagttg
     5941 ccaggttgga gagatgagag gagccaatga aaatagcaag cctgtttgac aactcaaccc
     6001 ttaaaagaga aagataaact cgcaattgta tgaagtcgtc ctatgagatg tcagaccctt
     6061 aaaataatac accttcatga gtatttttct atcaagttat atatgtaaga tgtaaaaata
     6121 aatttatttg ttgtatttca aagggccatt agaaaaaaac cttgataatt tatgtattca
     6181 ttctaaagta tatgcaatat ttatcacttc tgtgttgatg tactaacatt gttttgagca
     6241 cataaatata ttaatattgg ttttaaatgt gtaactttaa aaataattaa aacggcatta
     6301 ctgtgattat gatagagttg tgctttgctt aatgatagga atacattgtg cctttagacc
     6361 atattattat tgtacaaatg ttgttgggtg cacttaccca gactgagatg gctgtggcct
     6421 cattagacgg tatagcctac gggaccatct cctacaggtt gtcaatcact gacccaaagg
     6481 ccactgtgta gcacagtact gtgtatagat atgggctaag cacatgaaat ccttacttta
     6541 actacagttt tggcaaaccg ttattccatt ttactttata aatcttataa tgaatttcag
     6601 acaatcatta aagctgtctt tactgccagt gatatattga atttaaaaat gtgtttcaaa
     6661 ggtatgggat atatttatcc catgaattaa gatgaaaatt attcagcaca gagaacttga
     6721 gattttttta attcagcaaa actgtatgtg actaatagag aaaccaatat gtttctaaag
     6781 aaaagcctta gcattttgaa tttaataaat gatatgacct gcatttgcat atatacaaca
     6841 tgcacccata actttcaggg aattaatatt ttgctgccat cagcacacaa ccctgaagct
     6901 ctcatagttc aggctgtcct gtctgaaagt cttgagagta aggagagctg actgactgac
     6961 tgactaggca gacccttcac actgctttcc tgttctgtgc tcaccagcct cagtaagttt
     7021 ccatagaggc aaagaaaggg ctacatgagg gctacaaagc atgcttatat gtcctgggta
     7081 tattgtctac ttttgcatta tttataaatt agtgtatata acagattgtg gttaatgtta
     7141 tcttttgagt taaatatatt tgtgtgcaca gcacttacca actttaaaag tcggctggtg
     7201 gtttatggta atgtccatca ctgtcaattt catggtagtg aagtcatagt tgacatgatt
     7261 tagtgtgtac taaaaggctc cctttattgc ctactccacg tttcgctatt ccttcttcac
     7321 atggtggtgc agcagggatg aagcctggcc ttgtcttatt acgtgcactt cacagttcag
     7381 ctacagcaga gactaacgga gtgactcgcc caagttcacc tgatgaacat ggtggggtca
     7441 gatgatgaac atggcggagt cagaagttaa tgtaggaaat ccctcttctt aatgattaca
     7501 ctaatttcat ctgttaatat gagaaccatt ataatattag caattatttt ctgactaaaa
     7561 agttggagtt ttatgacatg tgttgtcatt caattctaca gactatgttg caaaaatcaa
     7621 gtgggaatat gcagaacacc tttaatgtat aagaactata ttcttggcac ttcccattca
     7681 taattgaaga ggccccacat gtattgcaag ccaagagtaa gacattatat aaaatttgac
     7741 tataatttta agattgctaa cacttaaaag tctacgatag atgaataatc aatttttaat
     7801 tccataatat ataccttctt ttgacattgg aattttagca ggccttagac cttttgaaca
     7861 gccttgcttt aatgtcggac tagcctctgc aactgggggt tactgagaag ctaagggaaa
     7921 agccctgctt actgttctta caacttctgt ttgtgaacat aggctcctgt ctacacactg
     7981 agagatttca gatcgcatca tgggaactga atcgtattta ctatcttaaa gatttttctc
     8041 atcgatttct aacattacaa aatttaattt tgtaaaaagt atgaagattt aagggctgaa
     8101 agtatgtcca ttccttattc tagagagaag gacagacagg ggatgatagt ttctcttctg
     8161 gttgacttgg caggatatgt aataatgagt gtatgctctc ccctgtaaac cagcatcgtc
     8221 atcagcaggc ccatgctact ctctttcatt taacagaagt cttctactct tcaatcccct
     8281 gtgtattcat tgtcactctt cttgcagcca gatgttcact taaaaaggaa ggtagggctg
     8341 gtaatgtggc acagtggttg agcacttgcc gagcacacgt gaggccatat attaaaccgt
     8401 gctgactctt cttcaggcca gtggtggccc acacctctaa tcccagtact tggagactca
     8461 gtaggattgc cagcagttca agggcacttt agctacatat gagtgtgagg ccagtctgaa
     8521 ctacagtgtg agacatggcc tatgaaggaa agaaagatac tacagtacat gttacttggg
     8581 gagggtgcta tttctggctt tattgatttt aactttgaat agttcaattc agaaggaaat
     8641 gaagttgatc cctaacttta acgatgaata taatcaataa gaaacaaatt caagaggaac
     8701 tattacccaa cttaagcaaa aaatgtcttg tagcgagcac atagatttta taggcaaggt
     8761 tatttaaagc aaatatgttt ctatctcaaa ctatcaagta tccttacaaa agtctaaaca
     8821 tctgtagtcc tgtgcattgt gagtcaccac atctaagcag gtgggttttt ttaggcactt
     8881 ggggttttat aaaatcataa tattaaaatt tcaaagtaat aaccttgctt tttaagtcat
     8941 ttgccctttt aaaaaatttt gaaagtaatt ctggaaatct acccagtggg attacagtca
     9001 aaccactaaa gctatgtttt tgactgaatt gcttctaaac tttaggaatt ttaattttga
     9061 aagtttaatt ttgtttttat gtgaatgttc ctgcaaaact gaggtccaaa gactcttcct
     9121 taaacaatta caatttcatt agcatacata agtttcaact aagtaagacg aactatactt
     9181 ttgtatattt gacgtttcag gggagagcct caggaaaatc tttactgtga agaacaggtg
     9241 ggggaaatct aagaggcagc tttaccaaga atggcctcat tatagtagca gttttaaaaa
     9301 atagtcacca ttttactagg tgcggcaatt ccatgacagt cataatggtt aatagttggt
     9361 tctagaatca tttgatactt taatgatttg ccattgtcac tattgttagc attgctgttg
     9421 ccaagcacca catggcatca gtgtggaggt ctattaaagt acagttagtc tgtgtgactc
     9481 tcagtggcca cttagtagga gtctgcctca ctcagaacag tttattctct ccctggctag
     9541 gtttctaaag tctaatgagt ttgataatga tatacagatg tgtgtgtgtg tgtgtgtgtg
     9601 tgtgtgtgtg tgtgtgtatt ctcacataac ataaaccatt tttttagttt tatgaacatt
     9661 aactataaac attatgataa ttattaaatt ttggtgtttt cttcatatga aaatacaagt
     9721 cacgtataga cagaccatat gatgtctgct gcagacgtca gtgctaccta ggccagaggt
     9781 gaacttgtgg gctgtcctat attcactgag gctcctctgc tatatgtatg agccagcttg
     9841 gtttctgatt tgtttttgtt tgttatcact gtgacaaatc atctgagata attaagttac
     9901 aaagagaaaa ggcttatttt ggctcaaatt gatggaggtt ctagtccaca aagcctgtgg
     9961 ttggacctgt catgaggtag caactcccga caggagaaga cagaagaaca taactagtgt
    10021 gctcattagc caaatcacaa aggtgattaa gagactttag ccccacagtg tttcaaaggc
    10081 agccttcaac agacatagga actatccatt ggcctccatc ttccagatgt ttcaccacct
    10141 ctcaatagca ctacagaggg gatcaagctc ttaatacagg acctttggga gacatgccgg
    10201 aatccaaact aaatatctag tcaatctcaa atctgctttt ctggtttacc attaagaatt
    10261 ataatggact taattgctga atttgtaaat gatagttgct agaaatactg gtaataccag
    10321 aaatcacaaa aattgggtaa ttaattttag gcacagataa tggtttgttt cattcatatt
    10381 caacaaacat tttgaaattc acttaataag gcccaaccag aatttagtgt attggatata
    10441 tttcattaaa aaaaaatcat catagggtca cttatatctt ttactttaaa attgtattat
    10501 tcataaggta aatatattct aataccattt gtgtattgta tactatatgg tcaagaatac
    10561 taagttgttg acatatttag aaagcataca cttgaattta aatatctaat gcctcaagca
    10621 aatctactag tttcagaaga atatatattt ttgtaactgc aagaatatga gtagatcagc
    10681 ttttgcttaa aatatttttg taagcactca tttacataat actcattttt aatagtaaaa
    10741 ttgattctgt agaagtaact gttttgacta tgtctatatt taatatgata taatatccaa
    10801 aattcaatga taaatttcat ctaaatgtat tgttagccct ttttcctttg taaaatagtc
    10861 atgtaacata gtttcagtca ttccatattt gtgtatggtt aaatttgtta aagttcatta
    10921 tgtattttgt atcattcgtt ttatcccaca taaaagtttg gaaaagtaat gttatctgat
    10981 aaatgtcaaa aataataaag tgaattatag agtgagctta a
//
