LOCUS       NM_001107315            4759 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Rattus norvegicus PEAK1 related kinase activating pseudokinase 1
            (Prag1), mRNA.
ACCESSION   NM_001107315 XM_001066723 XM_224908
VERSION     NM_001107315.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Lecointre,C., Simon,V., Kerneur,C., Allemand,F., Fournet,A.,
            Montarras,I., Pons,J.L., Gelin,M., Brignatz,C., Urbach,S.,
            Labesse,G. and Roche,S.
  TITLE     Dimerization of the Pragmin Pseudo-Kinase Regulates Protein
            Tyrosine Phosphorylation
  JOURNAL   Structure 26 (4), 545-554 (2018)
   PUBMED   29503074
  REMARK    Erratum:[Structure. 2018 Nov 6;26(11):1563. doi:
            10.1016/j.str.2018.10.020.. PMID: 30403993]
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Patel,O., Griffin,M.D.W., Panjikar,S., Dai,W., Ma,X., Chan,H.,
            Zheng,C., Kropp,A., Murphy,J.M., Daly,R.J. and Lucet,I.S.
  TITLE     Structure of SgK223 pseudokinase reveals novel mechanisms of
            homotypic and heterotypic association
  JOURNAL   Nat Commun 8 (1), 1157 (2017)
   PUBMED   29079850
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4759)
  AUTHORS   Senda,Y., Murata-Kamiya,N. and Hatakeyama,M.
  TITLE     C-terminal Src kinase-mediated EPIYA phosphorylation of Pragmin
            creates a feed-forward C-terminal Src kinase activation loop that
            promotes cell motility
  JOURNAL   Cancer Sci 107 (7), 972-980 (2016)
   PUBMED   27116701
REFERENCE   4  (bases 1 to 4759)
  AUTHORS   Weaver,K.L., Alves-Guerra,M.C., Jin,K., Wang,Z., Han,X.,
            Ranganathan,P., Zhu,X., DaSilva,T., Liu,W., Ratti,F.,
            Demarest,R.M., Tzimas,C., Rice,M., Vasquez-Del Carpio,R.,
            Dahmane,N., Robbins,D.J. and Capobianco,A.J.
  TITLE     NACK is an integral component of the Notch transcriptional
            activation complex and is critical for development and
            tumorigenesis
  JOURNAL   Cancer Res 74 (17), 4741-4751 (2014)
   PUBMED   25038227
REFERENCE   5  (bases 1 to 4759)
  AUTHORS   Safari,F., Murata-Kamiya,N., Saito,Y. and Hatakeyama,M.
  TITLE     Mammalian Pragmin regulates Src family kinases via the
            Glu-Pro-Ile-Tyr-Ala (EPIYA) motif that is exploited by bacterial
            effectors
  JOURNAL   Proc Natl Acad Sci U S A 108 (36), 14938-14943 (2011)
   PUBMED   21873224
REFERENCE   6  (bases 1 to 4759)
  AUTHORS   Tanaka,H., Katoh,H. and Negishi,M.
  TITLE     Pragmin, a novel effector of Rnd2 GTPase, stimulates RhoA activity
  JOURNAL   J Biol Chem 281 (15), 10355-10364 (2006)
   PUBMED   16481321
  REMARK    GeneRIF: Rnd2 regulates neurite outgrowth by functioning as the
            RhoA activator through pragmin, in contrast to Rnd1 and Rnd3
            inhibiting RhoA signaling
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CH473970.2.
            
            On or before Oct 4, 2007 this sequence version replaced
            XM_001066723.1, XM_224908.4.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN11044071,
                              SAMN12840113 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="16"
                     /map="16q12.2"
     gene            1..4759
                     /gene="Prag1"
                     /gene_synonym="Pragmin; RGD1311793"
                     /note="PEAK1 related kinase activating pseudokinase 1"
                     /db_xref="GeneID:306506"
                     /db_xref="RGD:1311793"
     exon            1..131
                     /gene="Prag1"
                     /gene_synonym="Pragmin; RGD1311793"
                     /inference="alignment:Splign:2.1.0"
     exon            132..553
                     /gene="Prag1"
                     /gene_synonym="Pragmin; RGD1311793"
                     /inference="alignment:Splign:2.1.0"
     CDS             263..4369
                     /gene="Prag1"
                     /gene_synonym="Pragmin; RGD1311793"
                     /EC_number="2.7.10.2"
                     /note="tyrosine-protein kinase SgK223; pragma of Rnd2"
                     /codon_start=1
                     /product="inactive tyrosine-protein kinase PRAG1"
                     /protein_id="NP_001100785.1"
                     /db_xref="GeneID:306506"
                     /db_xref="RGD:1311793"
                     /translation="MSACSDFVEHIWKPGSCKNCFCLRSDHQLTAGHPKARASSLPAG
                     ARLPARPEICRLEDEGVNGLAYSKPTIAVKPTMMTSETADLWTEASLSAEVPKVNWRR
                     TPGKLLLQKQEDGPIVYLGSFRGMQKAAGPLACTDSNSRCPPAYTMVGLHNLEARVDR
                     NTALQPVNFQEEKAGREELPSAQESFRQKLAAFTGMTSSCLKGPRPCTSPQPLRESLP
                     SEDDSDQRCSPSGDSEGGEYCSILDCRPESRDAVHNTEGSGRRRGDCSPICWEQGTCT
                     RPTEEEKQALNFPRECCGQGSTANPPHLGPKKPSLNSEAASSSDGLSCGSSRSGANSP
                     FAPHLENDYCSLVKEPTSVKQQDSGCHLVNSGKYVGQAVDLQPPALPREAVQPEPIYA
                     ESAKRKKAAPVPQRPEPKKEQVSSGQVWTGDTWSQKTPSGWSQEREGPNAAPQVATTI
                     TVIAAHPEEDHRTIYLSSPDSAVGVQWPRGSLNQDLHGSGEEPLVVQGLSSRESHPHN
                     MTENSSKEKPAIPPKLSKSSPGGSPVSPAAPPLTDHSDGNTSGSSVGSQPSSRVPTNL
                     TSSCQTNGVAAGDPAKCPPQANSSVLDQRRPRYQTGAWSRQCRIEEEEEVGQELLSQS
                     WGRELENGIADHSNSSTWHRLHPIDGASGQNGKTNSGMSKSASFAFEFPKDRGRLESF
                     SPPPPPPKSRHLLKMNKSSSDLEKVSQSSAESLSPSFRGAHVSFTTGSTDSLASDSRT
                     CSDGGPSCEATHSPTISGKKLFAPVPFPSGSTEDVSPSGPAQPPPLPQKKIVSRAASS
                     PDGFFWTQGSPKPRTASPKLNLSHSETNVCAHDEPPLSYSLNSGNHPHHVFSSSEPLE
                     KAFKGSVPWAPALGPANSKGGCGSPNLQGRAATSTSSSQLSVSSQASTGSSQLQLHSL
                     LSSISSKEGTYAKLGGLYTQSLARLVTKCEDLFMGGLKTELRFDENSWSLFKLICNKP
                     CCDSGDAIYYGATCSKDPDSIYAVKICKTPEPKSASYCSPSVPVHFNIQQDCGHFVAS
                     VPSSMLAFPDTSSKDPAPAAPSHTPAQEQDCVVVITREVPHQTASDFVRDSVASHRAE
                     PEVYERRVCFLLLQLCNGLEHLKEHGIIHRDLCLENLLLVHCNPQSSPGPSANPSVPT
                     TTSRCPSAAPAATTACQGGPGEKHLPRLIISNFLKAKQKPGGTTNLQQKKSQARLAPE
                     IVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLRERDYRREDLPPLPTLSLYSPGL
                     QQLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVEQPCPSEEVLCNTLHNWIDMK
                     RALMMMKFAEKAVERRRGVELEDWLCCQYLASAEPGALLQSLKLLQLL"
     exon            554..2337
                     /gene="Prag1"
                     /gene_synonym="Pragmin; RGD1311793"
                     /inference="alignment:Splign:2.1.0"
     exon            2338..2495
                     /gene="Prag1"
                     /gene_synonym="Pragmin; RGD1311793"
                     /inference="alignment:Splign:2.1.0"
     exon            2496..3253
                     /gene="Prag1"
                     /gene_synonym="Pragmin; RGD1311793"
                     /inference="alignment:Splign:2.1.0"
     exon            3254..4759
                     /gene="Prag1"
                     /gene_synonym="Pragmin; RGD1311793"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcagaagtca gccgcccgcg gctgggcaag cactgcgcgc agacacgcta cagacaatgc
       61 tccgggacgc ccggcggggc agcggaccgc tgggccaccg acggcgaagc tgcggaaccg
      121 cagctcccca gggagttgat ctggaactca tctctgcacc ccctgtaaga ccgaaacaga
      181 gagccgggcc caagatgaac tcgcacctta tcagcggctc acagtgctcc agaccgtttg
      241 actgaacccc gagagcccga aaatgtctgc gtgcagtgac tttgtggagc acatctggaa
      301 acccggatcc tgcaagaact gcttctgctt gaggagtgac catcagctca cagctggcca
      361 ccccaaagct agagccagca gcctacccgc tggagctcgc ctgcctgcca ggcctgagat
      421 ttgccgctta gaggatgaag gtgtgaatgg cttggcctac tcaaagccca ccattgcggt
      481 aaagcccacc atgatgactt ctgagactgc cgacttgtgg actgaggcca gcctgagcgc
      541 tgaagttcca aaggtcaact ggagacgaac tccaggcaag ctcctgctcc agaaacagga
      601 agatgggcca atagtttacc tcggcagctt ccgaggaatg cagaaggctg caggccctct
      661 cgcctgcaca gatagcaact ctcgttgtcc cccagcctac accatggtcg gactgcataa
      721 cctggaggcc cgagtggata ggaacaccgc cctccaacca gtgaatttcc aggaagagaa
      781 ggctgggagg gaagagctgc cctcagctca ggagagcttt cgtcagaagc tagctgcctt
      841 tacagggatg acgtccagct gcctcaaggg ccccagaccc tgcacttctc cacagccctt
      901 gcgggagtcc ctgccctcag aggatgacag tgaccaaagg tgctcaccct caggagacag
      961 cgaaggtggg gagtactgct ccattttgga ctgtcgtcca gaatccagag atgctgtgca
     1021 taacaccgag ggctctggcc gacggcgagg ggactgctca cccatatgct gggagcaggg
     1081 aacatgtaca agaccaacag aagaggaaaa gcaggctctg aacttcccca gagagtgttg
     1141 tggtcaggga tccacagcaa acccacccca cctgggcccc aagaagccgt cccttaactc
     1201 agaggctgcc agctcttcag atggtctgtc ctgtggcagc agccgcagtg gtgccaacag
     1261 cccctttgct ccacacctgg agaatgacta ctgctctctt gtgaaggagc caacctcggt
     1321 gaagcagcag gactctggct gccacttagt caactctggc aaatatgtgg gtcaagccgt
     1381 ggacctccag cccccagccc ttcctaggga ggctgtgcag cctgaaccca tctacgccga
     1441 aagtgccaag aggaagaagg cagctccggt gcctcagagg cccgagccca agaaagagca
     1501 ggtctcgtct ggccaagtat ggacaggcga tacctggtct cagaagacac cctctggctg
     1561 gagccaggaa agggaaggcc caaacgctgc tcctcaggtg gcaaccacta ttactgttat
     1621 tgctgctcac ccagaagagg accataggac tatttatctg agcagcccag actctgccgt
     1681 gggggtgcag tggccacgtg ggtctttgaa ccaggactta catggtagtg gagaagagcc
     1741 tttggtcgtg caggggctaa gctcaaggga gagccatcca cataatatga cagaaaactc
     1801 gtccaaagaa aagcctgcca taccccccaa gctgtccaaa agcagcccgg gagggtcccc
     1861 ggtgtcacct gcagcccccc ctctgactga ccacagtgat gggaacacca gtggcagcag
     1921 tgtcggatct cagccttcgt ctagagtccc tactaaccta acttcttcct gccagaccaa
     1981 tggtgttgcc gccggtgacc ctgccaagtg tcccccacaa gccaactctt cagtcttgga
     2041 ccagaggcgg ccaagatacc agactggtgc atggagccgc cagtgccgga tagaggagga
     2101 ggaagaggtg gggcaggagt tgttgagtca aagttggggg agagagttgg aaaatggtat
     2161 tgcagaccat tcaaactcct ctacctggca ccggctccat cccattgatg gcgcctctgg
     2221 gcagaacggc aaaactaact ctgggatgag caaatcagct tcctttgcgt ttgagttccc
     2281 aaaggacaga ggcagattgg agtcattctc accccctccc ccacctccaa agtcaaggca
     2341 cctgttaaaa atgaacaaga gcagctctga cttggagaaa gtgagccaga gctctgcaga
     2401 aagcctcagc ccgtccttcc gaggggccca cgtcagcttc accacgggct ccacagatag
     2461 cctagcctcg gactccagga cttgcagcga tggaggtcca tcgtgtgaag ccacccactc
     2521 acccaccatc agcgggaaaa agctctttgc tcccgtccct ttcccgtcag gctccacgga
     2581 ggatgtgtcc cccagcggtc ctgcacagcc tcctcctctg ccccagaaaa agatagtgag
     2641 ccgtgctgcc tcctcccctg atggcttctt ctggacccaa ggttccccta agccacgcac
     2701 agcgagtccc aagctgaacc taagccactc ggaaaccaat gtttgtgccc atgacgagcc
     2761 tcctttgagc tattccttga actctggaaa ccatccccac catgtcttct cctcttctga
     2821 gcctttggag aaagctttca aaggcagtgt cccctgggcc ccagctctgg gtccagcaaa
     2881 cagcaaaggc ggttgtggga gccccaatct ccagggcaga gcggccacct ccacctcgtc
     2941 atcccagctc agcgtgtcca gccaggcctc caccggcagc agccagcttc agctgcacag
     3001 cctgctgagc agtatcagca gcaaggaggg cacctatgcc aagctcgggg gcctctacac
     3061 acagtccctg gcccgcctag tgaccaagtg cgaagacctc ttcatgggtg gtctgaaaac
     3121 ggaactgcgc ttcgatgaga acagctggtc gctcttcaaa ctgatatgca acaagccttg
     3181 ctgtgactcg ggagatgcaa tttactacgg tgccacttgc tccaaggacc ccgacagcat
     3241 ctacgcagtg aaaatctgca aaaccccaga acccaagtca gcttcgtact gtagcccgtc
     3301 cgtgccagtg cacttcaaca tccagcaaga ctgcggccac tttgtggcct ctgtgccctc
     3361 cagcatgctt gccttccccg acacatccag caaggaccct gcgcctgctg cgccctcaca
     3421 cacccctgcc caggagcagg actgtgtggt ggtcatcacc cgggaggtgc cgcaccagac
     3481 tgcctcggac tttgtgcggg actctgtggc cagccaccgc gcagagcctg aggtttacga
     3541 gcgccgggtg tgcttcttgc ttctgcagct ctgcaacggg ctggagcatc tgaaggaaca
     3601 tgggatcatc caccgggacc tatgtctgga gaacctgttg ctagtgcatt gcaaccctca
     3661 gagctctccc gggccctctg ctaatcccag cgtgcccacc accacgtccc gttgtccttc
     3721 tgccgcaccc gcagccacca cagcctgcca gggaggacct ggtgagaagc acctgcccag
     3781 gctgatcatc agcaacttcc tgaaggccaa gcagaagccg ggaggcacca ccaacctgca
     3841 acagaagaag agccaggccc ggctggcccc tgagatcgta tctgcctctc agtaccgtaa
     3901 gtttgatgag ttccagactg gcatcctcat ctacgagctg ctccaccagc ccaacccatt
     3961 cgaggtgcga gcccagctac gggagcgtga ctaccggcgg gaggacctac ccccgctgcc
     4021 cacactgtcg ctctactcgc ccggcctgca gcagctcgcc cacctgctcc ttgaggccga
     4081 tcccatcaag cgcatccgca taggtgaggc caagcgggtg ctacagtgct tgctgtgggg
     4141 gccacggcga gagctggtag agcagccgtg cccctcggag gaggtactgt gcaacactct
     4201 gcacaactgg atcgacatga agcgagccct gatgatgatg aagtttgccg agaaggcggt
     4261 agaacgaagg cggggtgtgg agctggagga ttggctctgt tgccagtact tggcctcagc
     4321 agagccaggg gccctcttgc agtccctgaa gctcctgcaa cttctctgag cccctagtca
     4381 gaccattccc tcgccacctt ccatgcccgc cccatgccct tgccttgcct gcatccgggt
     4441 taccctggga aatggtacaa atgactatcg tccttggatg taatatttta tatatacata
     4501 tataatataa atatgaaaga ctgggagtgt cacttcacac acgccagcct cctctcctca
     4561 cagagtcccc cttcctccac caccgccacc ctgtaattat gtacagttct agcttcacgc
     4621 gaggtcctga ggacagtccc accgatagaa ccaagtttgg actcctgcct cactacccca
     4681 ttatggagtc tgctcgccct tttgtcaaac tgttatttaa aaaacaacct ccacaataaa
     4741 aaaacaaagt cttatatta
//
