LOCUS       NM_001108050            3822 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus protein phosphatase 4, regulatory subunit 3A
            (Ppp4r3a), transcript variant 2, mRNA.
ACCESSION   NM_001108050 XM_001065644 XM_001065761 XM_001065812 XM_001065868
            XM_001065924 XM_001065977 XM_001066034 XM_001066094 XM_234484
VERSION     NM_001108050.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Yoon,Y.S., Lee,M.W., Ryu,D., Kim,J.H., Ma,H., Seo,W.Y., Kim,Y.N.,
            Kim,S.S., Lee,C.H., Hunter,T., Choi,C.S., Montminy,M.R. and
            Koo,S.H.
  TITLE     Suppressor of MEK null (SMEK)/protein phosphatase 4 catalytic
            subunit (PP4C) is a key regulator of hepatic gluconeogenesis
  JOURNAL   Proc Natl Acad Sci U S A 107 (41), 17704-17709 (2010)
   PUBMED   20876121
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000006.1 and FQ211523.1.
            
            On Jan 18, 2018 this sequence version replaced NM_001108050.2.
            
            Transcript Variant: This variant (2) has an alternate splice site
            in the coding region, compared to variant 1. The resulting isoform
            (2) lacks an internal segment, compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from non-reference
            strain transcript and genomic sequence data to make the sequence
            consistent with the reference genome assembly. The genomic
            coordinates used for the transcript record were based on transcript
            alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360186.1704805.1,
                                           SRR26643298.36829.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-360               JAXUCZ010000006.1  126073791-126074150 c
            361-416             JAXUCZ010000006.1  126058597-126058652 c
            417-515             JAXUCZ010000006.1  126054647-126054745 c
            516-584             JAXUCZ010000006.1  126051575-126051643 c
            585-735             FQ211523.1         915-1065
            736-1133            JAXUCZ010000006.1  126051026-126051423 c
            1134-1211           JAXUCZ010000006.1  126048400-126048477 c
            1212-1328           JAXUCZ010000006.1  126048179-126048295 c
            1329-1445           JAXUCZ010000006.1  126046510-126046626 c
            1446-1577           JAXUCZ010000006.1  126044539-126044670 c
            1578-1680           JAXUCZ010000006.1  126044336-126044438 c
            1681-1839           JAXUCZ010000006.1  126042680-126042838 c
            1840-2009           JAXUCZ010000006.1  126037361-126037530 c
            2010-2152           JAXUCZ010000006.1  126036364-126036506 c
            2153-2343           JAXUCZ010000006.1  126034999-126035189 c
            2344-2570           JAXUCZ010000006.1  126034372-126034598 c
            2571-3822           JAXUCZ010000006.1  126032068-126033319 c
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q32"
     gene            1..3822
                     /gene="Ppp4r3a"
                     /gene_synonym="RGD1309059; Smek1"
                     /note="protein phosphatase 4, regulatory subunit 3A"
                     /db_xref="GeneID:314388"
                     /db_xref="RGD:1309059"
     exon            1..360
                     /gene="Ppp4r3a"
                     /gene_synonym="RGD1309059; Smek1"
                     /inference="alignment:Splign:2.1.0"
     CDS             219..2681
                     /gene="Ppp4r3a"
                     /gene_synonym="RGD1309059; Smek1"
                     /note="isoform 2 is encoded by transcript variant 2;
                     serine/threonine-protein phosphatase 4 regulatory subunit
                     3A; SMEK homolog 1, suppressor of mek1"
                     /codon_start=1
                     /product="serine/threonine-protein phosphatase 4
                     regulatory subunit 3A isoform 2"
                     /protein_id="NP_001101520.2"
                     /db_xref="GeneID:314388"
                     /db_xref="RGD:1309059"
                     /translation="MTDTRRRVKVYTLNEDRQWDDRGTGHVSSGYVERLKGMSLLVRA
                     ESDGSLLLESKINPNTAYQKQQDTLIVWSEAENYDLALSFQEKAGCDEIWEKICQVQG
                     KDPSVDITQDLVDESEEERFDDMSSPGLELPSCELSRLEEVAELVASSLPSPLRREKL
                     ALALENEGYIKKLLELFHVCEDLENIEGLHHLYEIIKGIFLLNRTALFEVMFSEECIM
                     DVIGCLEYDPTLSQPRKHREFLTKTAKFKEVIPISDPELKQKIHQTYRVQYIQDMVLP
                     TPSVFEENMLSTLHSFIFFNKVEIVGMLQEDEKFLTDLFAQLTDEATDEEKRQELVNF
                     LKEFCAFSQTLQPQNRDAFFKTLSNMGILPALEVILGMDDTQVRSAATDIFSYLVEYN
                     PSMVREFVMQEAQQNDDDILLINLIIEHMICDTDPELGGAVQLMGLLRTLVDPENMLA
                     TANKTEKTEFLGFFYKHCMHVLTAPLLANTTEDKPSKDDFQTAQLLALVLELLTFCVE
                     HHTYHIKNYIINKDILRRVLVLMASKHAFLALCALRFKRKIIGLKDEFYNRYIMKSFL
                     FEPVVKAFLNNGSRYNLMNSAIIEMFEFIRVEDIKSLTAHVIENYWKALEDVDYVQTF
                     KGLKLRFEQQRERQDNPKLDSMRSILRNHRYRRDARTLEDEEEMWFNTDEDDMEDGEA
                     VVSPSDKTKNDDDIMDPISKFMERKKLKESEEKEVLLKTNLSGRQSPSFKLSLSSGTK
                     TNLTSQSSATSLPGSPGSPGSPGSPGSPGSVPKSTSQPAAITTKGGLVGLVDYPDDDE
                     DDDEDEDKEDTLPVSKKAKFES"
     exon            361..416
                     /gene="Ppp4r3a"
                     /gene_synonym="RGD1309059; Smek1"
                     /inference="alignment:Splign:2.1.0"
     exon            417..515
                     /gene="Ppp4r3a"
                     /gene_synonym="RGD1309059; Smek1"
                     /inference="alignment:Splign:2.1.0"
     exon            516..1133
                     /gene="Ppp4r3a"
                     /gene_synonym="RGD1309059; Smek1"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..1211
                     /gene="Ppp4r3a"
                     /gene_synonym="RGD1309059; Smek1"
                     /inference="alignment:Splign:2.1.0"
     exon            1212..1328
                     /gene="Ppp4r3a"
                     /gene_synonym="RGD1309059; Smek1"
                     /inference="alignment:Splign:2.1.0"
     exon            1329..1445
                     /gene="Ppp4r3a"
                     /gene_synonym="RGD1309059; Smek1"
                     /inference="alignment:Splign:2.1.0"
     exon            1446..1577
                     /gene="Ppp4r3a"
                     /gene_synonym="RGD1309059; Smek1"
                     /inference="alignment:Splign:2.1.0"
     exon            1578..1680
                     /gene="Ppp4r3a"
                     /gene_synonym="RGD1309059; Smek1"
                     /inference="alignment:Splign:2.1.0"
     exon            1681..1839
                     /gene="Ppp4r3a"
                     /gene_synonym="RGD1309059; Smek1"
                     /inference="alignment:Splign:2.1.0"
     exon            1840..2009
                     /gene="Ppp4r3a"
                     /gene_synonym="RGD1309059; Smek1"
                     /inference="alignment:Splign:2.1.0"
     exon            2010..2152
                     /gene="Ppp4r3a"
                     /gene_synonym="RGD1309059; Smek1"
                     /inference="alignment:Splign:2.1.0"
     exon            2153..2343
                     /gene="Ppp4r3a"
                     /gene_synonym="RGD1309059; Smek1"
                     /inference="alignment:Splign:2.1.0"
     exon            2344..2570
                     /gene="Ppp4r3a"
                     /gene_synonym="RGD1309059; Smek1"
                     /inference="alignment:Splign:2.1.0"
     exon            2571..3822
                     /gene="Ppp4r3a"
                     /gene_synonym="RGD1309059; Smek1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cggccgcgct cactcggcct ggacaatagc gccgaggaga gggagacgag agaaggcggc
       61 ggcccggggc tcggacccgg gagcgggcca tgcgagcggc ggcgcagctc cgcggcggcc
      121 agcggcggca ctgaggtctc ggaggccccc gtttacggcc gcccgctttt cctggacacc
      181 gtccctgggc cactcgggcg gggcccccgg tcggcacgat gaccgacacc cgacggcggg
      241 tgaaggtcta cacgctcaac gaggaccggc agtgggacga ccggggtact ggccatgtgt
      301 cctccggcta cgtggagagg ctgaagggca tgtccctgct cgtcagggca gagagcgacg
      361 gttctcttct tctagagtcc aaaataaacc ctaacactgc ataccagaaa caacaggaca
      421 ctctgattgt gtggtctgaa gcagaaaatt atgacttggc ccttagcttt caagaaaaag
      481 ctggatgtga tgaaatttgg gaaaaaatat gtcaggttca aggaaaggac ccttcagtgg
      541 acatcactca ggacctggta gatgagtctg aggaggagcg attcgatgac atgtcctcgc
      601 caggtctaga gctgccatcg tgtgagctga gccggcttga ggaggttgca gagcttgtgg
      661 cttcgtcctt accttccccc ctccggcggg agaaacttgc actcgcactg gagaacgagg
      721 gttatattaa aaagctttta gagcttttcc atgtgtgtga ggatctggaa aacattgaag
      781 gactgcacca cttatatgag attatcaaag ggatcttcct cttgaatcga actgctcttt
      841 ttgaagttat gttctctgag gaatgtataa tggacgtcat tggatgcttg gaatatgacc
      901 caactttatc acaaccacga aaacacaggg aatttttaac aaaaacagct aagtttaaag
      961 aagtaattcc catatcagat cctgagctga aacaaaaaat tcatcaaaca tacagagttc
     1021 agtatataca agacatggtt ctccctaccc cttcagtctt tgaagaaaac atgttatcaa
     1081 cacttcactc ctttatcttt ttcaacaagg tagaaattgt tggtatgtta caggaagatg
     1141 aaaaatttct gacagatttg tttgcacaac taacagatga agcaacagat gaggaaaaaa
     1201 gacaagaatt ggttaacttt ttaaaagaat tttgtgcatt ttctcaaacg ctacaacccc
     1261 aaaatagaga tgcttttttc aagactttgt caaatatggg catattacca gctttagaag
     1321 tcatccttgg catggatgat acacaggtgc gaagtgctgc tactgatata ttctcatact
     1381 tggttgaata taacccatcc atggtacgag agtttgtcat gcaggaggca caacagaatg
     1441 atgatgatat tttgctcatc aacctcatta tagaacatat gatttgtgat acagatccag
     1501 agcttggagg agcagtccaa cttatgggcc tgcttcgaac tttagttgac ccagagaaca
     1561 tgctagccac tgccaataaa acagaaaaga ctgaatttct gggtttcttc tacaagcact
     1621 gtatgcatgt tctcactgct cccttactag caaatacaac agaagataag cctagtaaag
     1681 atgattttca gactgcgcag ttactagcac ttgtattgga attgctaaca ttttgtgtgg
     1741 agcaccatac ctaccacata aagaactata ttatcaataa ggacattctt cgcagagtgc
     1801 tagttcttat ggcctccaag catgctttct tggcgttatg tgccctccgt tttaagagaa
     1861 aaatcattgg attaaaagat gaattttata accgctacat aatgaaaagt tttttatttg
     1921 aacctgtagt caaagcattt ctcaacaacg gatctcgtta caatctgatg aactccgcca
     1981 taatagaaat gtttgaattt atcagagtgg aagatataaa atcattaact gcccatgtaa
     2041 ttgagaatta ttggaaagca ctagaagatg tagattatgt gcagactttt aaaggattaa
     2101 aattgaggtt tgaacagcaa agagaaaggc aagacaaccc aaaactcgac agcatgcggt
     2161 ccatcttgag gaatcatcga taccgacgag atgccaggac actagaagat gaagaagaga
     2221 tgtggttcaa cacagatgaa gatgacatgg aagacggaga agctgtggtg tctccatctg
     2281 acaaaaccaa gaatgatgac gatattatgg atccaataag taaatttatg gaaaggaaga
     2341 aattgaaaga gagtgaagaa aaggaggtgc ttctgaaaac taatctttct ggacgacaga
     2401 gcccgagttt caaactttcc ttgtccagtg ggacaaagac taacctcacc agccagtcat
     2461 ccgccacaag cctgcctggt tctccaggat cacctgggtc cccaggctct cctggctctc
     2521 ctggatccgt ccctaagagt acatctcagc cggcagctat taccacaaag ggaggacttg
     2581 tgggtctggt agattatcct gatgatgatg aagatgatga tgaggatgaa gacaaggaag
     2641 acacactacc agtgtcaaag aaagcaaaat ttgagtccta ataatagcaa ctgcctgtga
     2701 tcagtacctg ttgacaaaac tggttcttca cccctccccc tacaaagtcc acaccagagt
     2761 gcagtggtct cttgtgaagg actgacagat cagccacact ggacttctgc tcatcaagtg
     2821 ccaattcaaa ggagcaggag gagggggatg ccgtgcagtt aggggaagac atacgcccgt
     2881 gagctctcca gcttggacca cacattgccc ttttctcagg gaaggaaatg gaaataaaaa
     2941 gccaacaggg caggggtttt gtcagtggaa ctctggattg actggtcagt tgctacaatc
     3001 aaaacctgct ttcttggacc acgtttgaga cttagaagaa tgggtctttc tgccatagtt
     3061 cctcactagt aaagaatgcc agcagtttct tggtataaag agacctgcct ttaaaatcgt
     3121 acattctgaa cattttagtc aagctacaac aggtttggaa aacctttgtg ggggaggggc
     3181 aaaataaagt atcctctttt tttaatctgt tccctttgcc cttcaaactg cagatttttt
     3241 ttcaaggtgg ggatttgtcc ctacttgatt aaagattgag tggaattcta gatgtggtca
     3301 tttgtgtcat aatttttctt tttgtttcag tttgtttctg ttttgttttt tctctctccc
     3361 tgagtgtatg attagttgtt gaatatatat atttgggacc attaaaactt ttttgatgta
     3421 atatagccta acgtgctggc acctgcttta catgtgtaat ttgtgttcta catcacagtt
     3481 cttaatttgt ttagagtttt atgaaagatg gtatagtttt tattgacaaa agcaaagtaa
     3541 tcttacaact atgtgcatac aaaagcaata ctattttgtg actaaatatt ttatattaaa
     3601 atttgcatca gcagctgtct tgagatttca gggaaataga gtggaattta aaacttcaac
     3661 agttttgtta aacctagaaa catgaaatta gtattccaaa gagattcgga aatttcatat
     3721 cttggagaaa tgacggtaca ttaaatcaaa attgaggatg gatgatttaa aagtaatact
     3781 tgacttttga ataataaaaa gaaaagtgaa gagtaagaga aa
//
