LOCUS       NM_130403                538 bp    mRNA    linear   ROD 27-MAR-2024
DEFINITION  Rattus norvegicus protein phosphatase 1, regulatory (inhibitor)
            subunit 14A (Ppp1r14a), mRNA.
ACCESSION   NM_130403
VERSION     NM_130403.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Dong,Y., Wang,J., Yang,C., Bao,J., Liu,X., Chen,H., Zhang,X.,
            Shi,W., Zhang,L., Qi,Q., Li,Y., Wang,S., Ma,R., Cong,B. and
            Zhang,G.
  TITLE     Phosphorylated CPI-17 and MLC2 as Biomarkers of Coronary Artery
            Spasm-Induced Sudden Cardiac Death
  JOURNAL   Int J Mol Sci 25 (5), 2941 (2024)
   PUBMED   38474189
  REMARK    GeneRIF: Phosphorylated CPI-17 and MLC2 as Biomarkers of Coronary
            Artery Spasm-Induced Sudden Cardiac Death.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  AUTHORS   Kitazawa,T. and Kitazawa,K.
  TITLE     Prolonged bed rest impairs rapid CPI-17 phosphorylation and
            contraction in rat mesenteric resistance arteries to cause
            orthostatic hypotension
  JOURNAL   Pflugers Arch 469 (12), 1651-1662 (2017)
   PUBMED   28717991
  REMARK    GeneRIF: an impairment of the rapid CPI-17 signaling pathway
            mediating alpha1A-adrenoceptor activation is a cause of
            hypo-responsiveness of splanchnic resistance arteries, as an end
            organ of orthostatic blood pressure reflex, in the orthostatic
            hypotension model rats
REFERENCE   3  (bases 1 to 538)
  AUTHORS   Hou,J.Y., Wang,Y.G., Ma,S.J., Yang,B.Y. and Li,Q.P.
  TITLE     Identification of a prognostic 5-Gene expression signature for
            gastric cancer
  JOURNAL   J Cancer Res Clin Oncol 143 (4), 619-629 (2017)
   PUBMED   28035468
REFERENCE   4  (bases 1 to 538)
  AUTHORS   Shibata,K., Sakai,H., Huang,Q., Kamata,H., Chiba,Y., Misawa,M.,
            Ikebe,R. and Ikebe,M.
  TITLE     Rac1 regulates myosin II phosphorylation through regulation of
            myosin light chain phosphatase
  JOURNAL   J Cell Physiol 230 (6), 1352-1364 (2015)
   PUBMED   25502873
  REMARK    GeneRIF: Rac1 coordinates with RhoA to increase myosin light chain
            (MLC) phosphorylation by inactivation of CPI-17/MYPT1 phosphatase,
            which decreases MLC phosphatase activity thus promoting MLC
            phosphorylation and cell contraction
REFERENCE   5  (bases 1 to 538)
  AUTHORS   Lim,K.M., Kwon,J.H., Kim,K., Noh,J.Y., Kang,S., Park,J.M.,
            Lee,M.Y., Bae,O.N. and Chung,J.H.
  TITLE     Emodin inhibits tonic tension through suppressing PKCdelta-mediated
            inhibition of myosin phosphatase in rat isolated thoracic aorta
  JOURNAL   Br J Pharmacol 171 (18), 4300-4310 (2014)
   PUBMED   24909118
  REMARK    GeneRIF: Emodin attenuates tonic tension through the blockade of
            PKCdelta and CPI-17-mediated MLC-phosphatase inhibition.
REFERENCE   6  (bases 1 to 538)
  AUTHORS   Pang,H., Guo,Z., Xie,Z., Su,W. and Gong,M.C.
  TITLE     Divergent kinase signaling mediates agonist-induced phosphorylation
            of phosphatase inhibitory proteins PHI-1 and CPI-17 in vascular
            smooth muscle cells
  JOURNAL   Am J Physiol Cell Physiol 290 (3), C892-C899 (2006)
   PUBMED   16267107
  REMARK    GeneRIF: Agonists induce PHI-1 phosphorylation in VSMCs and
            divergent kinase signaling couples agonist stimulation to PHI-1 and
            CPI-17 phosphorylation.
REFERENCE   7  (bases 1 to 538)
  AUTHORS   Liu,Q.R., Zhang,P.W., Lin,Z., Li,Q.F., Woods,A.S., Troncoso,J. and
            Uhl,G.R.
  TITLE     GBPI, a novel gastrointestinal- and brain-specific PP1-inhibitory
            protein, is activated by PKC and inactivated by PKA
  JOURNAL   Biochem J 377 (Pt 1), 171-181 (2004)
   PUBMED   12974676
REFERENCE   8  (bases 1 to 538)
  AUTHORS   Deng,J.T., Sutherland,C., Brautigan,D.L., Eto,M. and Walsh,M.P.
  TITLE     Phosphorylation of the myosin phosphatase inhibitors, CPI-17 and
            PHI-1, by integrin-linked kinase
  JOURNAL   Biochem J 367 (Pt 2), 517-524 (2002)
   PUBMED   12144526
  REMARK    GeneRIF: ILK may activate smooth-muscle contraction both directly,
            via phosphorylation of myosin, and indirectly, via phosphorylation
            and activation of CPI-17 and PHI-1, leading to inhibition of myosin
            light-chain phosphatase
REFERENCE   9  (bases 1 to 538)
  AUTHORS   Takizawa,N., Koga,Y. and Ikebe,M.
  TITLE     Phosphorylation of CPI17 and myosin binding subunit of type 1
            protein phosphatase by p21-activated kinase
  JOURNAL   Biochem Biophys Res Commun 297 (4), 773-778 (2002)
   PUBMED   12359219
  REMARK    GeneRIF: phosphorylated by p21-activated kinase
REFERENCE   10 (bases 1 to 538)
  AUTHORS   Li,Z., Yu,L., Zhang,Y., Gao,J., Zhang,P., Wan,B., Chen,C. and
            Zhao,S.
  TITLE     Identification of human, mouse and rat PPP1R14A, protein
            phosphatase-1 inhibitor subunit 14A, & mapping human PPP1R14A to
            chromosome 19q13.13-q13.2
  JOURNAL   Mol Biol Rep 28 (2), 91-101 (2001)
   PUBMED   11931393
  REMARK    GeneRIF: distribution of mouse and rat PPP1R14A was more specific
            and different from that of humans
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Dec 1, 2020 this sequence version replaced NM_130403.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BQ211385.1, FQ230520.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-223               JAXUCZ010000001.1  93714102-93714324
            224-304             JAXUCZ010000001.1  93716300-93716380
            305-337             JAXUCZ010000001.1  93716833-93716865
            338-538             JAXUCZ010000001.1  93718018-93718218
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q21"
     gene            1..538
                     /gene="Ppp1r14a"
                     /gene_synonym="CPI-17; Cpi17"
                     /note="protein phosphatase 1, regulatory (inhibitor)
                     subunit 14A"
                     /db_xref="GeneID:114004"
                     /db_xref="RGD:620536"
     exon            1..223
                     /gene="Ppp1r14a"
                     /gene_synonym="CPI-17; Cpi17"
                     /inference="alignment:Splign:2.1.0"
     CDS             23..466
                     /gene="Ppp1r14a"
                     /gene_synonym="CPI-17; Cpi17"
                     /note="17 kDa PKC-potentiated inhibitory protein of PP1;
                     protein kinase C-potentiated inhibitor protein of 17 kDa"
                     /codon_start=1
                     /product="protein phosphatase 1 regulatory subunit 14A"
                     /protein_id="NP_569087.1"
                     /db_xref="GeneID:114004"
                     /db_xref="RGD:620536"
                     /translation="MAAQRLGKRVLSKLQSPSRARGPGGSPSGLQKRHARVTVKYDRR
                     ELQRRLDVEKWIDGRLEELYRGREADMPDEVNIDELLELDSEEERCRKIRGLLEACLN
                     PTEDFVQELLAKLRGLHKQPGFPQPSPSDDPSLSPRQDPAHTAPP"
     exon            224..304
                     /gene="Ppp1r14a"
                     /gene_synonym="CPI-17; Cpi17"
                     /inference="alignment:Splign:2.1.0"
     exon            305..337
                     /gene="Ppp1r14a"
                     /gene_synonym="CPI-17; Cpi17"
                     /inference="alignment:Splign:2.1.0"
     exon            338..538
                     /gene="Ppp1r14a"
                     /gene_synonym="CPI-17; Cpi17"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcagacagcg gacggcggcg tgatggcagc gcagcggctg ggcaagcgcg tgctgagcaa
       61 gctgcagtcc ccttcgcgtg cccgcggccc ggggggcagc cctagtgggc tgcagaagcg
      121 gcacgcgcga gtcaccgtca aatacgaccg gcgagagctg cagcggcgac tggacgtgga
      181 gaagtggatc gacggacgct tggaagagct gtaccgcggc agggaggcag acatgccaga
      241 tgaggtcaac atcgacgagc tgctggaatt ggacagtgag gaggaaagat gccggaaaat
      301 ccggggactc ttggaggctt gcctgaatcc cacagaggac ttcgtccagg agctattggc
      361 caagcttcgg ggcctgcaca agcagccggg cttcccgcag cccagcccct cagatgaccc
      421 cagcctgagc ccccgccagg acccggctca cactgctcca ccgtgactgc gcccgcctct
      481 gctccacccc caaagcccag attgtttcta agttgtattt aatggttccg taactggc
//
