LOCUS       NM_053890               3301 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus protein phosphatase 1, regulatory subunit 12A
            (Ppp1r12a), mRNA.
ACCESSION   NM_053890
VERSION     NM_053890.2
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Chiu,W.C., Chiang,J.Y., Juang,J.M., Wu,C.K., Tsai,C.T., Tseng,Y.Z.,
            Su,M.J. and Chiang,F.T.
  TITLE     Reduction of blood pressure elevation by losartan in spontaneously
            hypertensive rats through suppression of LARG expression in
            vascular smooth muscle cells
  JOURNAL   J Formos Med Assoc 119 (1 Pt 1), 164-172 (2020)
   PUBMED   30962047
  REMARK    GeneRIF: reduction of blood pressure elevation by losartan in
            spontaneously hypertensive rats occurs through the suppression of
            LARG expression and MYPT-1 phosphorylation in vascular smooth
            muscle cells
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Sutherland,C., MacDonald,J.A. and Walsh,M.P.
  TITLE     Analysis of phosphorylation of the myosin-targeting subunit of
            myosin light chain phosphatase by Phos-tag SDS-PAGE
  JOURNAL   Am J Physiol Cell Physiol 310 (8), C681-C691 (2016)
   PUBMED   26864694
  REMARK    GeneRIF: Phos-tag SDS-PAGE thus provides a suitable and convenient
            method for analysis of the complex, multisite MYPT1 phosphorylation
            events involved in the regulation of myosin light chain phosphatase
            activity and smooth muscle contraction.
REFERENCE   3  (bases 1 to 3301)
  AUTHORS   Alp Yildirim,F.I., Kaleli Durman,D., Aypar,E., Ark,M., Ozdemir,O.
            and Uydes Dogan,B.S.
  TITLE     Atorvastatin acutely reduces the reactivity to spasmogens in rat
            aorta: implication of the inhibition of geranylgeranylation and
            MYPT-1 phosphorylation
  JOURNAL   Fundam Clin Pharmacol 30 (2), 96-106 (2016)
   PUBMED   26610064
  REMARK    GeneRIF: Both atorvastatin (10(-4) m) and Y-27632 (10(-6) m)
            pretreatment inhibited the phosphorylation of myosin phosphatase
            target subunit-1 (MYPT-1).
REFERENCE   4  (bases 1 to 3301)
  AUTHORS   Sasahara,T., Okamoto,H., Ohkura,N., Kobe,A. and Yayama,K.
  TITLE     Epidermal growth factor induces Ca(2+) sensitization through
            Rho-kinase-dependent phosphorylation of myosin phosphatase target
            subunit 1 in vascular smooth muscle
  JOURNAL   Eur J Pharmacol 762, 89-95 (2015)
   PUBMED   26004531
  REMARK    GeneRIF: EGF induces Ca(2+) sensitization in vascular smooth muscle
            by Rho-kinase-dependent inactivation of MLCP mediated by the EGF
            receptor/MEK/Erk1/2 pathway
REFERENCE   5  (bases 1 to 3301)
  AUTHORS   Shioda,N., Sawai,M., Ishizuka,Y., Shirao,T. and Fukunaga,K.
  TITLE     Nuclear Translocation of Calcium/Calmodulin-dependent Protein
            Kinase IIdelta3 Promoted by Protein Phosphatase-1 Enhances
            Brain-derived Neurotrophic Factor Expression in Dopaminergic
            Neurons
  JOURNAL   J Biol Chem 290 (35), 21663-21675 (2015)
   PUBMED   26163515
  REMARK    GeneRIF: nuclear translocation of CaMKIIdelta3 following
            dephosphorylation at Ser(332) by PP1 likely accounts for BDNF
            expression and subsequent neurite extension and survival of
            dopaminergic neurons.
REFERENCE   6  (bases 1 to 3301)
  AUTHORS   Arimura,T., Suematsu,N., Zhou,Y.B., Nishimura,J., Satoh,S.,
            Takeshita,A., Kanaide,H. and Kimura,A.
  TITLE     Identification, characterization, and functional analysis of
            heart-specific myosin light chain phosphatase small subunit
  JOURNAL   J Biol Chem 276 (9), 6073-6082 (2001)
   PUBMED   11067852
REFERENCE   7  (bases 1 to 3301)
  AUTHORS   Kawano,Y., Fukata,Y., Oshiro,N., Amano,M., Nakamura,T., Ito,M.,
            Matsumura,F., Inagaki,M. and Kaibuchi,K.
  TITLE     Phosphorylation of myosin-binding subunit (MBS) of myosin
            phosphatase by Rho-kinase in vivo
  JOURNAL   J Cell Biol 147 (5), 1023-1038 (1999)
   PUBMED   10579722
REFERENCE   8  (bases 1 to 3301)
  AUTHORS   Kimura,K., Ito,M., Amano,M., Chihara,K., Fukata,Y., Nakafuku,M.,
            Yamamori,B., Feng,J., Nakano,T., Okawa,K., Iwamatsu,A. and
            Kaibuchi,K.
  TITLE     Regulation of myosin phosphatase by Rho and Rho-associated kinase
            (Rho-kinase)
  JOURNAL   Science 273 (5272), 245-248 (1996)
   PUBMED   8662509
REFERENCE   9  (bases 1 to 3301)
  AUTHORS   Haystead,C.M., Gailly,P., Somlyo,A.P., Somlyo,A.V. and
            Haystead,T.A.
  TITLE     Molecular cloning and functional expression of a recombinant 72.5
            kDa fragment of the 110 kDa regulatory subunit of smooth muscle
            protein phosphatase 1M
  JOURNAL   FEBS Lett 377 (2), 123-127 (1995)
   PUBMED   8543033
REFERENCE   10 (bases 1 to 3301)
  AUTHORS   Chen,Y.H., Chen,M.X., Alessi,D.R., Campbell,D.G., Shanahan,C.,
            Cohen,P. and Cohen,P.T.
  TITLE     Molecular cloning of cDNA encoding the 110 kDa and 21 kDa
            regulatory subunits of smooth muscle protein phosphatase 1M
  JOURNAL   FEBS Lett 356 (1), 51-55 (1994)
   PUBMED   7988720
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000007.1.
            
            On Nov 27, 2020 this sequence version replaced NM_053890.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA5760383,
                              SAMEA5760386 [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-327               JAXUCZ010000007.1  45369444-45369770
            328-458             JAXUCZ010000007.1  45405369-45405499
            459-577             JAXUCZ010000007.1  45420100-45420218
            578-737             JAXUCZ010000007.1  45431859-45432018
            738-882             JAXUCZ010000007.1  45435066-45435210
            883-957             JAXUCZ010000007.1  45440843-45440917
            958-1046            JAXUCZ010000007.1  45441514-45441602
            1047-1204           JAXUCZ010000007.1  45441739-45441896
            1205-1329           JAXUCZ010000007.1  45443542-45443666
            1330-1545           JAXUCZ010000007.1  45449515-45449730
            1546-1640           JAXUCZ010000007.1  45451332-45451426
            1641-1745           JAXUCZ010000007.1  45452377-45452481
            1746-1925           JAXUCZ010000007.1  45453819-45453998
            1926-2016           JAXUCZ010000007.1  45459845-45459935
            2017-2217           JAXUCZ010000007.1  45460970-45461170
            2218-2389           JAXUCZ010000007.1  45461358-45461529
            2390-2511           JAXUCZ010000007.1  45462551-45462672
            2512-2577           JAXUCZ010000007.1  45464491-45464556
            2578-2594           JAXUCZ010000007.1  45467713-45467729
            2595-2730           JAXUCZ010000007.1  45468993-45469128
            2731-2835           JAXUCZ010000007.1  45469638-45469742
            2836-2883           JAXUCZ010000007.1  45472426-45472473
            2884-2934           JAXUCZ010000007.1  45474812-45474862
            2935-3301           JAXUCZ010000007.1  45477737-45478103
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q21"
     gene            1..3301
                     /gene="Ppp1r12a"
                     /gene_synonym="M110; MBSP; Mypt1"
                     /note="protein phosphatase 1, regulatory subunit 12A"
                     /db_xref="GeneID:116670"
                     /db_xref="RGD:620013"
     exon            1..327
                     /gene="Ppp1r12a"
                     /gene_synonym="M110; MBSP; Mypt1"
                     /inference="alignment:Splign:2.1.0"
     CDS             91..3021
                     /gene="Ppp1r12a"
                     /gene_synonym="M110; MBSP; Mypt1"
                     /note="PP-1M; protein phosphatase subunit 1M; myosin
                     phosphatase-targeting subunit 1; protein phosphatase
                     myosin-binding subunit; serine/threonine protein
                     phosphatase PP1 smooth muscle regulatory subunit M110;
                     myosin phosphatase, target subunit 1; smooth muscle myosin
                     phosphatase myosin-binding subunit; protein phosphatase 1,
                     regulatory (inhibitor) subunit 12A"
                     /codon_start=1
                     /product="protein phosphatase 1 regulatory subunit 12A"
                     /protein_id="NP_446342.2"
                     /db_xref="GeneID:116670"
                     /db_xref="RGD:620013"
                     /translation="MKMADAKQKRNEQLKRWIGSETDLEPPVVKRQKTKVKFDDGAVF
                     LAACSSGDTDEVLKLLHRGADINYANVDGLTALHQACIDDNVDMVKFLVENGANINQP
                     DNEGWIPLHAAASCGYLDIAEFLIGQGAHVGAVNSEGDTPLDIAEEEAMEELLQNEVN
                     RQGVDIEAARKEEERIMLRDARQWLNSGHISDVRHAKSGGTALHVAAAKGYTEVLKLL
                     IQAGYDVNIKDYDGWTPLHAAAHWGKEEACRILVDNLCDMETVNKVGQTAFDVADEDI
                     LGYLEELQKKQNLLHSEKRDKKSPLIESTANMENNQPQKTFKNKETLIIEPEKNASRI
                     ESLEQEKADEEEEGKKDESSCSSEEDEEDDSESEAETDKTKPMASVTNAHTASTQAAP
                     AAVTTPTLSSNQGTPTSPVKKFPTSTTKISPKEEERKDESPASWRLGLRKTGSYGALA
                     EITASKEAQKEKDTAGVIRSASSPRLSSSLDNKEKEKDNKGTRLAYVAPTIPRRLGST
                     SDIEEKENRESSNLRTSSSYTRRKWEDDLKKNSSINEGSTYHRSTSNRLWAEDSTEKE
                     KDSAPTAATIPVAPTVVSAAASSTTALTTTTAGTLSSTSEVRERRRSYLTPVRDEESE
                     SQRKARSRQARQSRRSTQGVTLTDLQEAEKTIGRSRSTRTREQENEEKDKEEKEKQDK
                     EKQEEKKESEVSREDEYKQKYSRTYDETYARYRPVSTSSSSTPSSSSLSTLGSSLYAS
                     SQLNRPNSLVGITSAYSRGLTKDNEREGEKKEEEKEGEDKSQPKSIRERRRPREKRRS
                     TGVSFWTQDSDENEQERQSDTEDGSSKRDTQTDSVSRYDSSSTSSSDRYDSLLGRSAS
                     YSYLEERKPYGSRLEKDDSTDFKKLYEQILAENEKLKAQLHDTNMELTDLKLQLEKAT
                     QRQERFADRSLLEMEKRERRALERRISEMEEELKMLPDLKADNQRLKDENGALIRVIS
                     KLSK"
     exon            328..458
                     /gene="Ppp1r12a"
                     /gene_synonym="M110; MBSP; Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            459..577
                     /gene="Ppp1r12a"
                     /gene_synonym="M110; MBSP; Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            578..737
                     /gene="Ppp1r12a"
                     /gene_synonym="M110; MBSP; Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            738..882
                     /gene="Ppp1r12a"
                     /gene_synonym="M110; MBSP; Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            883..957
                     /gene="Ppp1r12a"
                     /gene_synonym="M110; MBSP; Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            958..1046
                     /gene="Ppp1r12a"
                     /gene_synonym="M110; MBSP; Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1047..1204
                     /gene="Ppp1r12a"
                     /gene_synonym="M110; MBSP; Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1205..1329
                     /gene="Ppp1r12a"
                     /gene_synonym="M110; MBSP; Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1330..1545
                     /gene="Ppp1r12a"
                     /gene_synonym="M110; MBSP; Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1546..1640
                     /gene="Ppp1r12a"
                     /gene_synonym="M110; MBSP; Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1641..1745
                     /gene="Ppp1r12a"
                     /gene_synonym="M110; MBSP; Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1746..1925
                     /gene="Ppp1r12a"
                     /gene_synonym="M110; MBSP; Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1926..2016
                     /gene="Ppp1r12a"
                     /gene_synonym="M110; MBSP; Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            2017..2217
                     /gene="Ppp1r12a"
                     /gene_synonym="M110; MBSP; Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            2218..2389
                     /gene="Ppp1r12a"
                     /gene_synonym="M110; MBSP; Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            2390..2511
                     /gene="Ppp1r12a"
                     /gene_synonym="M110; MBSP; Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            2512..2577
                     /gene="Ppp1r12a"
                     /gene_synonym="M110; MBSP; Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            2578..2594
                     /gene="Ppp1r12a"
                     /gene_synonym="M110; MBSP; Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            2595..2730
                     /gene="Ppp1r12a"
                     /gene_synonym="M110; MBSP; Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            2731..2835
                     /gene="Ppp1r12a"
                     /gene_synonym="M110; MBSP; Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            2836..2883
                     /gene="Ppp1r12a"
                     /gene_synonym="M110; MBSP; Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            2884..2934
                     /gene="Ppp1r12a"
                     /gene_synonym="M110; MBSP; Mypt1"
                     /inference="alignment:Splign:2.1.0"
     exon            2935..3301
                     /gene="Ppp1r12a"
                     /gene_synonym="M110; MBSP; Mypt1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cggtcgcaca ccccccggcg tcccctcgcc tccctcgccg ccgccccctt cccccgctcg
       61 cgataagaag agccggcggc aggagagggg atgaagatgg cggacgcgaa gcagaagcgg
      121 aacgagcagc tgaagcgctg gatcggctcc gagacggacc tcgagcctcc cgtggtgaag
      181 cgccagaaga ccaaggtgaa gttcgacgat ggcgccgtct tcctcgccgc ctgctccagc
      241 ggcgacacgg acgaggttct caagctgctg caccgcggcg ccgacatcaa ttacgccaat
      301 gtggacggac tcaccgccct gcaccaggct tgcattgatg acaatgttga tatggtgaag
      361 tttctggtag aaaatggagc aaatatcaat caacctgaca atgaaggctg gattccactc
      421 catgcagccg cttcctgtgg atatctggat attgcagaat ttttgattgg tcaaggagca
      481 catgtaggag ctgtcaacag tgaaggtgac acacctttag atattgcaga ggaggaagca
      541 atggaagagc tacttcaaaa tgaggttaat cggcaaggtg ttgatataga agcagctcga
      601 aaagaagagg aacgcataat gcttagagac gcgaggcagt ggttgaacag tggtcacatc
      661 agtgacgtcc ggcatgcaaa gtccggaggc acagcactcc acgtggcagc ggccaaaggg
      721 tatacagaag ttttaaaact tttaatacag gcaggctatg atgttaatat taaagattat
      781 gatggctgga cacctcttca tgctgcagct cactggggta aagaagaagc atgtcggatt
      841 ttagtggaca atctgtgtga tatggagacg gtcaacaaag tgggccaaac agcctttgat
      901 gtagcagatg aagacatttt gggatatcta gaggagttgc aaaaaaaaca aaatctgctc
      961 catagtgaaa agcgggataa gaaatctcca ctgattgaat caacagcaaa tatggaaaat
     1021 aatcaaccac agaagacttt taaaaacaag gaaacgttga ttattgagcc agagaaaaat
     1081 gcatctcgaa tcgagtctct ggagcaagaa aaggctgatg aggaggagga aggcaagaag
     1141 gatgagtcca gctgctccag tgaggaggat gaggaggatg actccgagtc cgaagcggag
     1201 acagataaga caaaacccat ggcttctgta actaatgctc acactgccag cactcaggca
     1261 gctcctgccg ctgtgacaac acctactctg tcttccaacc aggggacccc tacatcacct
     1321 gttaaaaagt ttcctacatc aactacaaaa atttctccca aagaagaaga aagaaaagat
     1381 gaatctcctg catcctggag gttaggactt agaaagactg gcagttatgg tgccctggct
     1441 gagatcactg catctaaaga agctcagaag gagaaagaca ctgcaggcgt gatacgttca
     1501 gcttcgagtc ccagactctc gtcctctttg gataataaag aaaaggagaa agacaataaa
     1561 ggaacaagac ttgcatatgt cgcccctaca atcccaaggc gactaggcag tacgtctgac
     1621 attgaagaga aggaaaacag agagtcttca aatttgcgaa caagtagttc ttacacaaga
     1681 agaaaatggg aagatgatct taaaaaaaat agttcaatca atgaaggatc tacttaccat
     1741 agaagtacct caaatcgttt gtgggctgag gatagtactg agaaagagaa ggacagtgct
     1801 cctaccgcag cgaccattcc tgttgctcca actgttgtaa gtgctgcagc ttcttctacc
     1861 acagccctga ccacaactac tgctggcact ctttcctcca catcagaggt cagggagaga
     1921 cgcaggtcat acctcactcc tgttagggat gaagagtctg aatcccaaag gaaagcaaga
     1981 tctagacaag caagacagtc tagacggtca acacaggggg tgacactgac tgacctccag
     2041 gaagccgaaa agacaatagg aagaagccgt tctacgagaa ccagagaaca agaaaacgaa
     2101 gaaaaagaca aagaagaaaa ggaaaagcag gataaagaga aacaagaaga aaagaaggag
     2161 tcagaagtat ctagagaaga tgaatataag caaaagtatt ccagaacata cgatgagact
     2221 tatgcacgtt acagaccagt gtcaacttca agttcaagca ctccgtcgtc ctcctcactt
     2281 tctactctag gcagttcact ctatgcctca agtcagctca acaggccaaa cagccttgta
     2341 ggtataacct ctgcctactc ccggggatta accaaagaca atgaaagaga gggagagaaa
     2401 aaagaagagg aaaaagaagg ggaagataag tcacaaccta aatcaatcag agaacgacgg
     2461 cgaccaagag aaaaacggag gtctactgga gtctccttct ggacacaaga tagtgatgaa
     2521 aatgagcaag agcggcagtc agacaccgag gatggctcca gcaagaggga cactcagacg
     2581 gattctgttt ccaggtatga cagcagttcc acgtcatcaa gcgatcggta tgactccttg
     2641 ctgggtcgtt ctgcctcata cagttactta gaagaaagga aaccatatgg tagccgacta
     2701 gaaaaggatg actcaactga cttcaaaaag ctttatgaac aaatcttagc tgaaaatgaa
     2761 aaactaaagg cacagctaca tgacacaaat atggaactaa cggatctaaa gttgcagttg
     2821 gaaaaagcta cccagagaca agaacgattt gctgacaggt cactattgga gatggaaaaa
     2881 agggaacgaa gagctctaga aagaagaata tctgagatgg aagaggagct caaaatgtta
     2941 ccagacttaa aagcagacaa ccagaggcta aaggatgaaa atggggcctt gatcagagtt
     3001 ataagcaaac tttccaagta ggacagaaaa cacacaagcg aagcagcggg acttgcacac
     3061 actccccagt ggaccacatt ggcagtcact ggacgccaga aagaacccct ggagactgtc
     3121 attttccgat atcctgccaa acgccctctt atctaggagt tttgtttcgt ttaatcttct
     3181 gccccacccc cttggttatc aagaccattg tttcatgtta aagccgctgc tgagaagatt
     3241 tttttttcaa tgactgagaa aacttgttta cagctccagc aaataaagaa agtgttcaag
     3301 g
//
