LOCUS       NM_001173355            1874 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus porcupine O-acyltransferase (Porcn), mRNA.
ACCESSION   NM_001173355 XM_001063037 XM_228781
VERSION     NM_001173355.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Erlenhardt,N., Yu,H., Abiraman,K., Yamasaki,T., Wadiche,J.I.,
            Tomita,S. and Bredt,D.S.
  TITLE     Porcupine Controls Hippocampal AMPAR Levels, Composition, and
            Synaptic Transmission
  JOURNAL   Cell Rep 14 (4), 782-794 (2016)
   PUBMED   26776514
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Rios-Esteves,J., Haugen,B. and Resh,M.D.
  TITLE     Identification of key residues and regions important for
            porcupine-mediated Wnt acylation
  JOURNAL   J Biol Chem 289 (24), 17009-17019 (2014)
   PUBMED   24798332
REFERENCE   3  (bases 1 to 1874)
  AUTHORS   Kurimoto,A., Kitazume,S., Kizuka,Y., Nakajima,K., Oka,R.,
            Fujinawa,R., Korekane,H., Yamaguchi,Y., Wada,Y. and Taniguchi,N.
  TITLE     The absence of core fucose up-regulates GnT-III and Wnt target
            genes: a possible mechanism for an adaptive response in terms of
            glycan function
  JOURNAL   J Biol Chem 289 (17), 11704-11714 (2014)
   PUBMED   24619415
REFERENCE   4  (bases 1 to 1874)
  AUTHORS   Das,S., Yu,S., Sakamori,R., Stypulkowski,E. and Gao,N.
  TITLE     Wntless in Wnt secretion: molecular, cellular and genetic aspects
  JOURNAL   Front Biol (Beijing) 7 (6), 587-593 (2012)
   PUBMED   23439944
REFERENCE   5  (bases 1 to 1874)
  AUTHORS   Schwenk,J., Harmel,N., Brechet,A., Zolles,G., Berkefeld,H.,
            Muller,C.S., Bildl,W., Baehrens,D., Huber,B., Kulik,A., Klocker,N.,
            Schulte,U. and Fakler,B.
  TITLE     High-resolution proteomics unravel architecture and molecular
            diversity of native AMPA receptor complexes
  JOURNAL   Neuron 74 (4), 621-633 (2012)
   PUBMED   22632720
REFERENCE   6  (bases 1 to 1874)
  AUTHORS   Takada,R., Satomi,Y., Kurata,T., Ueno,N., Norioka,S., Kondoh,H.,
            Takao,T. and Takada,S.
  TITLE     Monounsaturated fatty acid modification of Wnt protein: its role in
            Wnt secretion
  JOURNAL   Dev Cell 11 (6), 791-801 (2006)
   PUBMED   17141155
REFERENCE   7  (bases 1 to 1874)
  AUTHORS   Tanaka,K., Okabayashi,K., Asashima,M., Perrimon,N. and Kadowaki,T.
  TITLE     The evolutionarily conserved porcupine gene family is involved in
            the processing of the Wnt family
  JOURNAL   Eur J Biochem 267 (13), 4300-4311 (2000)
   PUBMED   10866835
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000021.1.
            
            On or before May 13, 2010 this sequence version replaced
            XM_228781.5, XM_001063037.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA5760386,
                              SAMEA5760393 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-127               JAXUCZ010000021.1  16957834-16957960
            128-296             JAXUCZ010000021.1  16958600-16958768
            297-489             JAXUCZ010000021.1  16959926-16960118
            490-533             JAXUCZ010000021.1  16960494-16960537
            534-715             JAXUCZ010000021.1  16960792-16960973
            716-846             JAXUCZ010000021.1  16961053-16961183
            847-864             JAXUCZ010000021.1  16961298-16961315
            865-879             JAXUCZ010000021.1  16962726-16962740
            880-1005            JAXUCZ010000021.1  16962836-16962961
            1006-1106           JAXUCZ010000021.1  16963138-16963238
            1107-1183           JAXUCZ010000021.1  16964841-16964917
            1184-1247           JAXUCZ010000021.1  16965003-16965066
            1248-1333           JAXUCZ010000021.1  16965165-16965250
            1334-1444           JAXUCZ010000021.1  16966222-16966332
            1445-1874           JAXUCZ010000021.1  16970011-16970440
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="X"
                     /map="Xq12"
     gene            1..1874
                     /gene="Porcn"
                     /gene_synonym="RGD1564947"
                     /note="porcupine O-acyltransferase"
                     /db_xref="GeneID:317368"
                     /db_xref="RGD:1564947"
     exon            1..127
                     /gene="Porcn"
                     /gene_synonym="RGD1564947"
                     /inference="alignment:Splign:2.1.0"
     exon            128..296
                     /gene="Porcn"
                     /gene_synonym="RGD1564947"
                     /inference="alignment:Splign:2.1.0"
     CDS             161..1546
                     /gene="Porcn"
                     /gene_synonym="RGD1564947"
                     /EC_number="2.3.1.250"
                     /note="protein-cysteine N-palmitoyltransferase porcupine;
                     porcupine homolog"
                     /codon_start=1
                     /product="protein-serine O-palmitoleoyltransferase
                     porcupine"
                     /protein_id="NP_001166826.1"
                     /db_xref="GeneID:317368"
                     /db_xref="RGD:1564947"
                     /translation="MATFSRQEFFQQLLQGCLLPTVQQGLDQIWLLLTICFACRLLWR
                     LGLPSYLKHASTVAGGFFSLYHFFQLHMVWVVLLSLLCYLVLFLCRHSSHRGVFLSVT
                     ILIYLLMGEMHMVDTVTWHKMRGAQMIVAMKAVSLGFDLDRGEVGAVPSPVEFMGYLY
                     FVGTIVFGPWISFHSYLQAVQGRPLSRRWLKKVARSLALALLCLVLSTCVGPYLFPYF
                     IPLDGDRLLRNKKRKARGTMVRWLRAYESAVSFHFSNYFVGFLSEATATLAGAGFTEE
                     KDHLEWDLTVSRPLNVELPRSMVEVVTSWNLPMSYWLNNYVFKNALRLGTFSAVLVTY
                     AASALLHGFSFHLAAVLLSLAFITYVEHVLRKRLAQILSACILSKRCLPDCSHRHRLG
                     LGVRALNLLFGALAVFHLAYLGSLFDVDVDDTTEEQGYGMAYTVHKWSELSWASHWVT
                     FGCWIFYRLIG"
     exon            297..489
                     /gene="Porcn"
                     /gene_synonym="RGD1564947"
                     /inference="alignment:Splign:2.1.0"
     exon            490..533
                     /gene="Porcn"
                     /gene_synonym="RGD1564947"
                     /inference="alignment:Splign:2.1.0"
     exon            534..715
                     /gene="Porcn"
                     /gene_synonym="RGD1564947"
                     /inference="alignment:Splign:2.1.0"
     exon            716..846
                     /gene="Porcn"
                     /gene_synonym="RGD1564947"
                     /inference="alignment:Splign:2.1.0"
     exon            847..864
                     /gene="Porcn"
                     /gene_synonym="RGD1564947"
                     /inference="alignment:Splign:2.1.0"
     exon            865..879
                     /gene="Porcn"
                     /gene_synonym="RGD1564947"
                     /inference="alignment:Splign:2.1.0"
     exon            880..1005
                     /gene="Porcn"
                     /gene_synonym="RGD1564947"
                     /inference="alignment:Splign:2.1.0"
     exon            1006..1106
                     /gene="Porcn"
                     /gene_synonym="RGD1564947"
                     /inference="alignment:Splign:2.1.0"
     exon            1107..1183
                     /gene="Porcn"
                     /gene_synonym="RGD1564947"
                     /inference="alignment:Splign:2.1.0"
     exon            1184..1247
                     /gene="Porcn"
                     /gene_synonym="RGD1564947"
                     /inference="alignment:Splign:2.1.0"
     exon            1248..1333
                     /gene="Porcn"
                     /gene_synonym="RGD1564947"
                     /inference="alignment:Splign:2.1.0"
     exon            1334..1444
                     /gene="Porcn"
                     /gene_synonym="RGD1564947"
                     /inference="alignment:Splign:2.1.0"
     exon            1445..1874
                     /gene="Porcn"
                     /gene_synonym="RGD1564947"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcgccgccct gggagccact gctggtcccg gccttgcggc ctgcggggga ggctgcccgg
       61 aggaggtggc gaaggtggca gcagtgcgcg tccccggccc ccaggaccac ggcttccttt
      121 ctgccagatc catctgtcct tccatctgtg tgggtccaca atggctacct tcagccgcca
      181 ggaatttttc cagcagctac tgcagggctg cctcctgcct actgtccaac agggccttga
      241 ccagatctgg ctgcttctta ccatctgctt cgcctgccgc ctcctttgga ggctggggct
      301 accgtcttac ctgaagcatg caagcaccgt ggcaggcggc ttcttcagcc tctaccactt
      361 cttccagctg cacatggttt gggtcgtgct gctgagtctc ctgtgctacc tcgtgctgtt
      421 cctctgccga cactcctccc accgaggcgt cttcctctcc gtcaccatcc tcatctacct
      481 gctcatgggt gagatgcaca tggtggacac cgtgacatgg cacaagatgc gaggggccca
      541 gatgatcgtg gccatgaagg cggtgtctct gggcttcgac ctggaccggg gcgaggtggg
      601 tgcagtgccc tcacctgtgg agttcatggg ctacctctac tttgtgggca ccatcgtctt
      661 tgggccctgg atatccttcc acagctacct acaggctgtc caaggccgcc cactgagccg
      721 ccgatggctg aagaaggtgg cccggagcct ggcgctggcc ctgctgtgcc ttgtactgtc
      781 cacttgtgtg ggcccctacc tcttccccta cttcatcccc ctcgacggtg accgactcct
      841 tcgcaacaag aaacgcaaag ccaggggcac catggtaagg tggctacgag cctacgagag
      901 tgctgtctcc ttccacttca gcaactattt tgtgggcttt ctgtctgagg ccacagccac
      961 attggccggg gctggcttca cggaggagaa ggaccacctc gaatgggacc tgacagtctc
     1021 tagaccattg aatgtggagc taccccggtc tatggtggaa gttgtcacaa gctggaacct
     1081 gcccatgtct tattggttaa ataattatgt tttcaaaaat gccctccgcc tggggacctt
     1141 ctcagctgtg ctggtcacct atgcagccag tgccctccta catggcttca gtttccacct
     1201 ggctgctgtg ctgctgtccc tggcatttat cacatatgtg gaacatgtcc tcaggaagcg
     1261 cctggctcag atcctcagtg cctgcatctt gtcgaagcgt tgtcttccag actgttcaca
     1321 tcggcatcgc ttgggcctgg gggtacgagc cttaaacttg ctctttggtg ccctggctgt
     1381 cttccacctg gcctacctgg gctccctgtt tgatgtcgat gtggacgaca ccacagagga
     1441 gcagggctac ggcatggcat acactgtcca caagtggtca gagctcagct gggccagtca
     1501 ctgggtcact tttggatgct ggatcttcta ccgtctcata ggctgaggca tatgatcttc
     1561 ataatcccct ccaatacccc tccttagggt gccaactctg atgggggatg agggaaggcc
     1621 ctttctctac tccttgaccc ctctccattc ttgagcccca acacccctct acacaacata
     1681 caaacacata caacctttcc atgcctgtca atcctcaccc catcacgagg caggaagggg
     1741 gtggtccttg ttgggaccta agaacggtgg atacttgggg aagcagagag gagtagatct
     1801 agggcctccg agcctgcctt ctcccttttt tatatgcagt ttgttattgt caaataaaag
     1861 tagaaattac acaa
//
