LOCUS       NM_031576               2472 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Rattus norvegicus cytochrome p450 oxidoreductase (Por), mRNA.
ACCESSION   NM_031576
VERSION     NM_031576.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ducharme,J., Sevrioukova,I.F., Thibodeaux,C.J. and Auclair,K.
  TITLE     Structural Dynamics of Cytochrome P450 3A4 in the Presence of
            Substrates and Cytochrome P450 Reductase
  JOURNAL   Biochemistry 60 (28), 2259-2271 (2021)
   PUBMED   34196520
  REMARK    GeneRIF: Structural Dynamics of Cytochrome P450 3A4 in the Presence
            of Substrates and Cytochrome P450 Reductase.
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Xia,C., Rwere,F., Im,S., Shen,A.L., Waskell,L. and Kim,J.P.
  TITLE     Structural and Kinetic Studies of Asp632 Mutants and Fully Reduced
            NADPH-Cytochrome P450 Oxidoreductase Define the Role of Asp632 Loop
            Dynamics in the Control of NADPH Binding and Hydride Transfer
  JOURNAL   Biochemistry 57 (6), 945-962 (2018)
   PUBMED   29308883
  REMARK    GeneRIF: The structure of four-electron-reduced, NADP(+)-bound wild
            type CYPOR shows the plane of the nicotinamide ring positioned
            perpendicular to the FAD isoalloxazine with its carboxamide group
            forming H-bonds with N1 of the flavin ring and the Thr535 hydroxyl
            group.
REFERENCE   3  (bases 1 to 2472)
  AUTHORS   Gonzalez,E. and Guengerich,F.P.
  TITLE     Kinetic processivity of the two-step oxidations of progesterone and
            pregnenolone to androgens by human cytochrome P450 17A1
  JOURNAL   J Biol Chem 292 (32), 13168-13185 (2017)
   PUBMED   28684414
  REMARK    GeneRIF: Data suggest CYP17A1, with electron donor NADPH-P450
            reductase, is inherently distributive enzyme but that some
            processivity is present; some 17alpha-hydroxy pregnenolone formed
            does not dissociate from CYP17A1 before conversion to
            dehydroepiandrosterone; CYB5A does not enhance reaction by
            decreasing k(off) of ligands of CYP17A1. (CYP17A1 = cytochrome
            P450, family 17, subfamily A, polypeptide 1; CYB5A = cytochrome b5)
REFERENCE   4  (bases 1 to 2472)
  AUTHORS   Hunter,S.R., Vonk,A., Mullen Grey,A.K. and Riddick,D.S.
  TITLE     Role of Glucocorticoid Receptor and Pregnane X Receptor in
            Dexamethasone Induction of Rat Hepatic Aryl Hydrocarbon Receptor
            Nuclear Translocator and NADPH-Cytochrome P450 Oxidoreductase
  JOURNAL   Drug Metab Dispos 45 (2), 118-129 (2017)
   PUBMED   27856527
REFERENCE   5  (bases 1 to 2472)
  AUTHORS   Rwere,F., Xia,C., Im,S., Haque,M.M., Stuehr,D.J., Waskell,L. and
            Kim,J.J.
  TITLE     Mutants of Cytochrome P450 Reductase Lacking Either Gly-141 or
            Gly-143 Destabilize Its FMN Semiquinone
  JOURNAL   J Biol Chem 291 (28), 14639-14661 (2016)
   PUBMED   27189945
  REMARK    GeneRIF: The DeltaGly-143 protein had moderately decreased activity
            with cytochrome P450 and cytochrome c. It formed a flexible loop,
            which transiently interacts with the flavin N5, resulting in the
            generation of both an unstable neutral blue semiquinone and
            hydroquinone.
REFERENCE   6  (bases 1 to 2472)
  AUTHORS   Wang,M., Roberts,D.L., Paschke,R., Shea,T.M., Masters,B.S. and
            Kim,J.J.
  TITLE     Three-dimensional structure of NADPH-cytochrome P450 reductase:
            prototype for FMN- and FAD-containing enzymes
  JOURNAL   Proc Natl Acad Sci U S A 94 (16), 8411-8416 (1997)
   PUBMED   9237990
REFERENCE   7  (bases 1 to 2472)
  AUTHORS   Porter,T.D., Beck,T.W. and Kasper,C.B.
  TITLE     NADPH-cytochrome P-450 oxidoreductase gene organization correlates
            with structural domains of the protein
  JOURNAL   Biochemistry 29 (42), 9814-9818 (1990)
   PUBMED   2125483
REFERENCE   8  (bases 1 to 2472)
  AUTHORS   Waxman,D.J., Morrissey,J.J. and Leblanc,G.A.
  TITLE     Hypophysectomy differentially alters P-450 protein levels and
            enzyme activities in rat liver: pituitary control of hepatic NADPH
            cytochrome P-450 reductase
  JOURNAL   Mol Pharmacol 35 (4), 519-525 (1989)
   PUBMED   2495435
REFERENCE   9  (bases 1 to 2472)
  AUTHORS   Murakami,H., Yabusaki,Y. and Ohkawa,H.
  TITLE     Expression of rat NADPH-cytochrome P-450 reductase cDNA in
            Saccharomyces cerevisiae
  JOURNAL   DNA 5 (1), 1-10 (1986)
   PUBMED   3082610
REFERENCE   10 (bases 1 to 2472)
  AUTHORS   Porter,T.D. and Kasper,C.B.
  TITLE     Coding nucleotide sequence of rat NADPH-cytochrome P-450
            oxidoreductase cDNA and identification of flavin-binding domains
  JOURNAL   Proc Natl Acad Sci U S A 82 (4), 973-977 (1985)
   PUBMED   3919392
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000012.1.
            
            On Nov 20, 2020 this sequence version replaced NM_031576.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360176.1199448.1,
                                           SRR26360190.586329.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760389,
                                           SAMEA5760393 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-61                JAXUCZ010000012.1  26635749-26635809   c
            62-253              JAXUCZ010000012.1  26606133-26606324   c
            254-302             JAXUCZ010000012.1  26595160-26595208   c
            303-431             JAXUCZ010000012.1  26593387-26593515   c
            432-581             JAXUCZ010000012.1  26592526-26592675   c
            582-706             JAXUCZ010000012.1  26591988-26592112   c
            707-796             JAXUCZ010000012.1  26591807-26591896   c
            797-895             JAXUCZ010000012.1  26591315-26591413   c
            896-1012            JAXUCZ010000012.1  26590422-26590538   c
            1013-1131           JAXUCZ010000012.1  26590215-26590333   c
            1132-1313           JAXUCZ010000012.1  26589323-26589504   c
            1314-1463           JAXUCZ010000012.1  26589079-26589228   c
            1464-1734           JAXUCZ010000012.1  26588704-26588974   c
            1735-1880           JAXUCZ010000012.1  26588478-26588623   c
            1881-1966           JAXUCZ010000012.1  26588303-26588388   c
            1967-2472           JAXUCZ010000012.1  26587674-26588179   c
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="12"
                     /map="12q12"
     gene            1..2472
                     /gene="Por"
                     /gene_synonym="CYPOR"
                     /note="cytochrome p450 oxidoreductase"
                     /db_xref="GeneID:29441"
                     /db_xref="RGD:68335"
     exon            1..61
                     /gene="Por"
                     /gene_synonym="CYPOR"
                     /inference="alignment:Splign:2.1.0"
     exon            62..253
                     /gene="Por"
                     /gene_synonym="CYPOR"
                     /inference="alignment:Splign:2.1.0"
     CDS             75..2111
                     /gene="Por"
                     /gene_synonym="CYPOR"
                     /EC_number="1.6.2.4"
                     /note="CPR; P450R; cytochrome P450 reductase; P450
                     (cytochrome) oxidoreductase"
                     /codon_start=1
                     /product="NADPH--cytochrome P450 reductase"
                     /protein_id="NP_113764.1"
                     /db_xref="GeneID:29441"
                     /db_xref="RGD:68335"
                     /translation="MGDSHEDTSATMPEAVAEEVSLFSTTDMVLFSLIVGVLTYWFIF
                     RKKKEEIPEFSKIQTTAPPVKESSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDA
                     HRYGMRGMSADPEEYDLADLSSLPEIDKSLVVFCMATYGEGDPTDNAQDFYDWLQETD
                     VDLTGVKFAVFGLGNKTYEHFNAMGKYVDQRLEQLGAQRIFELGLGDDDGNLEEDFIT
                     WREQFWPAVCEFFGVEATGEESSIRQYELVVHEDMDVAKVYTGEMGRLKSYENQKPPF
                     DAKNPFLAAVTANRKLNQGTERHLMHLELDISDSKIRYESGDHVAVYPANDSALVNQI
                     GEILGADLDVIMSLNNLDEESNKKHPFPCPTTYRTALTYYLDITNPPRTNVLYELAQY
                     ASEPSEQEHLHKMASSSGEGKELYLSWVVEARRHILAILQDYPSLRPPIDHLCELLPR
                     LQARYYSIASSSKVHPNSVHICAVAVEYEAKSGRVNKGVATSWLRAKEPAGENGGRAL
                     VPMFVRKSQFRLPFKSTTPVIMVGPGTGIAPFMGFIQERAWLREQGKEVGETLLYYGC
                     RRSDEDYLYREELARFHKDGALTQLNVAFSREQAHKVYVQHLLKRDREHLWKLIHEGG
                     AHIYVCGDARNMAKDVQNTFYDIVAEFGPMEHTQAVDYVKKLMTKGRYSLDVWS"
     exon            254..302
                     /gene="Por"
                     /gene_synonym="CYPOR"
                     /inference="alignment:Splign:2.1.0"
     exon            303..431
                     /gene="Por"
                     /gene_synonym="CYPOR"
                     /inference="alignment:Splign:2.1.0"
     exon            432..581
                     /gene="Por"
                     /gene_synonym="CYPOR"
                     /inference="alignment:Splign:2.1.0"
     exon            582..706
                     /gene="Por"
                     /gene_synonym="CYPOR"
                     /inference="alignment:Splign:2.1.0"
     exon            707..796
                     /gene="Por"
                     /gene_synonym="CYPOR"
                     /inference="alignment:Splign:2.1.0"
     exon            797..895
                     /gene="Por"
                     /gene_synonym="CYPOR"
                     /inference="alignment:Splign:2.1.0"
     exon            896..1012
                     /gene="Por"
                     /gene_synonym="CYPOR"
                     /inference="alignment:Splign:2.1.0"
     exon            1013..1131
                     /gene="Por"
                     /gene_synonym="CYPOR"
                     /inference="alignment:Splign:2.1.0"
     exon            1132..1313
                     /gene="Por"
                     /gene_synonym="CYPOR"
                     /inference="alignment:Splign:2.1.0"
     exon            1314..1463
                     /gene="Por"
                     /gene_synonym="CYPOR"
                     /inference="alignment:Splign:2.1.0"
     exon            1464..1734
                     /gene="Por"
                     /gene_synonym="CYPOR"
                     /inference="alignment:Splign:2.1.0"
     exon            1735..1880
                     /gene="Por"
                     /gene_synonym="CYPOR"
                     /inference="alignment:Splign:2.1.0"
     exon            1881..1966
                     /gene="Por"
                     /gene_synonym="CYPOR"
                     /inference="alignment:Splign:2.1.0"
     exon            1967..2472
                     /gene="Por"
                     /gene_synonym="CYPOR"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcctcagaga cctacaggac cgcgggccgt gggtgatctg gtcggtaccg gagagcgcag
       61 gttgtatcac caacatgggg gactctcacg aagacaccag tgccaccatg cctgaggccg
      121 tggctgaaga agtgtctcta ttcagcacga cggacatggt tctgttttct ctcatcgtgg
      181 gggtcctgac ctactggttc atctttagaa agaagaaaga agagataccg gagttcagca
      241 agatccaaac aacggcccca cccgtcaaag agagcagctt cgtggaaaag atgaagaaaa
      301 cgggaaggaa cattatcgta ttctatggct cccagacggg aaccgctgag gagtttgcca
      361 accggctgtc caaggatgcc caccgctacg ggatgcgggg catgtccgca gaccctgaag
      421 agtatgactt ggccgacctg agcagcctgc ctgagatcga caagtccctg gtagtcttct
      481 gcatggccac atacggagag ggcgacccca cggacaatgc gcaggacttc tatgactggc
      541 tgcaggagac tgacgtggac ctcactgggg tcaagtttgc tgtatttggt cttgggaaca
      601 agacctatga gcacttcaat gccatgggca agtatgtgga ccagaggctg gagcagcttg
      661 gcgcccagcg catctttgag ttgggccttg gtgatgatga cgggaacttg gaagaggatt
      721 tcatcacgtg gagggagcag ttctggccag ctgtgtgcga gttctttggg gtagaagcca
      781 ctggggagga gtcgagcatt cgccagtatg agctcgtggt ccacgaagac atggacgtag
      841 ccaaggtgta cacgggtgag atgggccgtc tgaagagcta cgagaaccag aaacccccct
      901 tcgatgctaa gaatccattc ctggctgctg tcaccgccaa ccggaagctg aaccaaggca
      961 ctgagcggca tctaatgcac ctggagttgg acatctcaga ctccaagatc aggtatgaat
     1021 ctggagatca cgtggctgtg tacccagcca atgactcagc cctggtcaac cagattgggg
     1081 agatcctggg agctgacctg gatgtcatca tgtctctaaa caatctcgat gaggagtcaa
     1141 acaagaagca tccgttcccc tgccccacca cctaccgcac ggccctcacc tactacctgg
     1201 acatcactaa cccgccacgc accaatgtgc tctacgaact ggcacagtac gcctcagagc
     1261 cctcggagca ggagcacctg cacaagatgg cgtcatcctc aggcgagggc aaggagctgt
     1321 acctgagctg ggtggtggaa gcccggaggc acatcctagc catcctccaa gactacccat
     1381 cactgcggcc acccatcgac cacctgtgtg agctgctgcc acgcctgcag gcccgatact
     1441 actccattgc ctcatcctcc aaggtccacc ccaactccgt gcacatctgt gccgtggccg
     1501 tggagtacga agcgaagtct ggccgagtga acaagggggt ggccactagc tggcttcggg
     1561 ccaaggaacc agcaggcgag aatggcggcc gcgccctggt acccatgttc gtgcgcaaat
     1621 ctcagttccg cttgcctttc aagtccacca cacctgtcat catggtgggc cccggcactg
     1681 ggattgcccc tttcatgggc ttcatccagg aacgagcttg gcttcgagag caaggcaagg
     1741 aggtgggaga gacgctgcta tactatggct gccggcgctc ggatgaggac tatctgtacc
     1801 gtgaagagct agcccgcttc cacaaggacg gtgccctcac gcagcttaat gtggcctttt
     1861 cccgggagca ggcccacaag gtctatgtcc agcaccttct gaagagagac agggaacacc
     1921 tgtggaagct gatccacgag ggcggtgccc acatctatgt gtgcggggat gctcgaaata
     1981 tggccaaaga tgtgcaaaac acattctatg acattgtggc tgagttcggg cccatggagc
     2041 acacccaggc tgtggactat gttaagaagc tgatgaccaa gggccgctac tcactagatg
     2101 tgtggagcta ggagctacca ccctcccacc cctcgctccc tgtaatcacc taacttctgc
     2161 cgacctccac ctctggtggt tcctgcctgg cctggacaca gggaggccca gggactgact
     2221 cctcctggcc tgagtggtgc cctcctgggc ccctaggcag agcccggtcc attgtatcag
     2281 gcagcccagc cccagggcac atggcaagag ggactggacc cacctttggg tgatgggtgc
     2341 cttaggtcct ctgcagctgt acagaagggg ctcttctctc cacagagctg gggtgcagcc
     2401 cccacacgtg attttgaatg agtgtaaata attttaaata acctggccct tggaataaag
     2461 ttgttttcag ta
//
