LOCUS       NM_001106002            3829 bp    mRNA    linear   ROD 26-FEB-2024
DEFINITION  Rattus norvegicus RNA polymerase II subunit B (Polr2b), mRNA.
ACCESSION   NM_001106002 XM_001075841 XM_214021
VERSION     NM_001106002.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Padmanabhan J, Brown KR, Padilla A and Shelanski ML.
  TITLE     Functional role of RNA polymerase II and P70 S6 kinase in KCl
            withdrawal-induced cerebellar granule neuron apoptosis
  JOURNAL   J Biol Chem 290 (9), 5267-5279 (2015)
   PUBMED   25568312
  REMARK    GeneRIF: Data indicate that activation of RNA polymerase II and p70
            S6 kinase (P70S6K) are increased in apoptotic neurons, and their
            inhibition prevents apoptosis.
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Knutson BA and Hahn S.
  TITLE     TFIIB-related factors in RNA polymerase I transcription
  JOURNAL   Biochim Biophys Acta 1829 (3-4), 265-273 (2013)
   PUBMED   22960599
  REMARK    Review article
REFERENCE   3  (bases 1 to 3829)
  AUTHORS   Grolimund L, Aeby E, Hamelin R, Armand F, Chiappe D, Moniatte M and
            Lingner J.
  TITLE     A quantitative telomeric chromatin isolation protocol identifies
            different telomeric states
  JOURNAL   Nat Commun 4, 2848 (2013)
   PUBMED   24270157
REFERENCE   4  (bases 1 to 3829)
  AUTHORS   Baltz AG, Munschauer M, Schwanhausser B, Vasile A, Murakawa Y,
            Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E,
            Bonneau R, Selbach M, Dieterich C and Landthaler M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   5  (bases 1 to 3829)
  AUTHORS   Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein
            C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J and
            Hentze MW.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   6  (bases 1 to 3829)
  AUTHORS   Canella D, Bernasconi D, Gilardi F, LeMartelot G, Migliavacca E,
            Praz V, Cousin P, Delorenzi M and Hernandez N.
  CONSRTM   CycliX Consortium
  TITLE     A multiplicity of factors contributes to selective RNA polymerase
            III occupancy of a subset of RNA polymerase III genes in mouse
            liver
  JOURNAL   Genome Res 22 (4), 666-680 (2012)
   PUBMED   22287103
REFERENCE   7  (bases 1 to 3829)
  AUTHORS   Ghosh D, Lippert D, Krokhin O, Cortens JP and Wilkins JA.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   8  (bases 1 to 3829)
  AUTHORS   Kershnar E, Wu SY and Chiang CM.
  TITLE     Immunoaffinity purification and functional characterization of
            human transcription factor IIH and RNA polymerase II from clonal
            cell lines that conditionally express epitope-tagged subunits of
            the multiprotein complexes
  JOURNAL   J Biol Chem 273 (51), 34444-34453 (1998)
   PUBMED   9852112
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH473981.1.
            
            On or before Oct 4, 2007 this sequence version replaced
            XM_001075841.1, XM_214021.4.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMD00132261, SAMD00132262
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="14"
                     /map="14p11"
     gene            1..3829
                     /gene="Polr2b"
                     /gene_synonym="RpB2"
                     /note="RNA polymerase II subunit B"
                     /db_xref="GeneID:289561"
                     /db_xref="RGD:1309477"
     exon            1..150
                     /gene="Polr2b"
                     /gene_synonym="RpB2"
                     /inference="alignment:Splign:2.1.0"
     CDS             132..3656
                     /gene="Polr2b"
                     /gene_synonym="RpB2"
                     /EC_number="2.7.7.6"
                     /note="polymerase (RNA) II (DNA directed) polypeptide B,
                     140kDa; DNA directed RNA polymerase II polypeptide B;
                     polymerase (RNA) II subunit B"
                     /codon_start=1
                     /product="DNA-directed RNA polymerase II subunit RPB2"
                     /protein_id="NP_001099472.1"
                     /db_xref="GeneID:289561"
                     /db_xref="RGD:1309477"
                     /translation="MYDADEDMQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQL
                     DSFDEFIQMSVQRIVEDAPPIDLQAEAQHASGEVEEPPRYLLKFEQIYLSKPTHWERD
                     GAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTY
                     CLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYT
                     GECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATLPYIKQEVPIIIVFRAL
                     GFVSDRDILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEK
                     RIKYAKEVLQKEMLPHVGVSDFCETKKAYFLGYMVHRLLLAALGRRELDDRDHYGNKR
                     LDLAGPLLAFLFRGMFKNLLKEVRIYAQKFIDRGKDFNLELAIKTRIISDGLKYSLAT
                     GNWGDQKKAHQARAGVSQVLNRLTFASTLSHLRRLNSPIGRDGKLAKPRQLHNTLWGM
                     VCPAETPEGHAVGLVKNLALMAYISVGSQPSPILEFLEEWSMENLEEISPAAIADATK
                     IFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRP
                     LLIVEKQKLLLKKRHIDQLKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDL
                     QEKEVAYCSTYTHCEIHPSMILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGVYITNF
                     HVRMDTLAHVLYYPQKPLVTTRSMEYLRFRELPAGINSIVAIASYTGYNQEDSVIMNR
                     SAVDRGFFRSVFYRSYKEQESKKGFDQEEVFEKPTRETCQGMRHAIYDKLDDDGLIAP
                     GVRVSGDDVIIGKTVTLPENEDELESTNRRYTKRDCSTFLRTSETGIVDQVMVTLNQE
                     GYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAI
                     PSRMTIGHLIECLQGKVSANKGEIGDATPFNDAVNVQKISNLLSDYGYHLRGNEVLYN
                     GFTGRKITSQIFIGPTYYQRLKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGE
                     MERDCQIAHGAAQFLRERLFEASDPYQVHVCNLCGIMAIANTRTHTYECRGCRNKTQI
                     SLVRMPYACKLLFQELMSMSIAPRMMSV"
     exon            151..223
                     /gene="Polr2b"
                     /gene_synonym="RpB2"
                     /inference="alignment:Splign:2.1.0"
     exon            224..374
                     /gene="Polr2b"
                     /gene_synonym="RpB2"
                     /inference="alignment:Splign:2.1.0"
     exon            375..487
                     /gene="Polr2b"
                     /gene_synonym="RpB2"
                     /inference="alignment:Splign:2.1.0"
     exon            488..707
                     /gene="Polr2b"
                     /gene_synonym="RpB2"
                     /inference="alignment:Splign:2.1.0"
     exon            708..866
                     /gene="Polr2b"
                     /gene_synonym="RpB2"
                     /inference="alignment:Splign:2.1.0"
     exon            867..1031
                     /gene="Polr2b"
                     /gene_synonym="RpB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1032..1228
                     /gene="Polr2b"
                     /gene_synonym="RpB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1229..1348
                     /gene="Polr2b"
                     /gene_synonym="RpB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..1535
                     /gene="Polr2b"
                     /gene_synonym="RpB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1536..1679
                     /gene="Polr2b"
                     /gene_synonym="RpB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1680..1819
                     /gene="Polr2b"
                     /gene_synonym="RpB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1820..1931
                     /gene="Polr2b"
                     /gene_synonym="RpB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1932..2086
                     /gene="Polr2b"
                     /gene_synonym="RpB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2087..2285
                     /gene="Polr2b"
                     /gene_synonym="RpB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2286..2454
                     /gene="Polr2b"
                     /gene_synonym="RpB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2455..2551
                     /gene="Polr2b"
                     /gene_synonym="RpB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2552..2646
                     /gene="Polr2b"
                     /gene_synonym="RpB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2647..2897
                     /gene="Polr2b"
                     /gene_synonym="RpB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2898..2987
                     /gene="Polr2b"
                     /gene_synonym="RpB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2988..3095
                     /gene="Polr2b"
                     /gene_synonym="RpB2"
                     /inference="alignment:Splign:2.1.0"
     exon            3096..3209
                     /gene="Polr2b"
                     /gene_synonym="RpB2"
                     /inference="alignment:Splign:2.1.0"
     exon            3210..3370
                     /gene="Polr2b"
                     /gene_synonym="RpB2"
                     /inference="alignment:Splign:2.1.0"
     exon            3371..3566
                     /gene="Polr2b"
                     /gene_synonym="RpB2"
                     /inference="alignment:Splign:2.1.0"
     exon            3567..3829
                     /gene="Polr2b"
                     /gene_synonym="RpB2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gctttgacgc cgaggattct gggaacgccg gagacggaaa ttacttcgtc tggcgctcag
       61 agtttgctgc agcgcttttc aactctagtt ttgtgatttc gagttaagag ctccagaaac
      121 ctttggccaa gatgtatgac gccgacgagg atatgcaata tgatgaagat gatgatgaaa
      181 tcactccaga tttgtggcag gaagcatgct ggatcgtgat cagttcttat tttgatgaaa
      241 aaggcttggt cagacagcag ctggattcct ttgacgagtt tattcagatg tctgttcaaa
      301 gaattgtgga agatgcacca ccgattgacc tgcaggctga agcccagcac gctagtgggg
      361 aggttgaaga gccgccaaga tatttactga agtttgaaca aatttacctc tccaaaccta
      421 ctcattggga aagagatggt gccccttcac caatgatgcc aaatgaagcc aggttaagaa
      481 atctcacgta ctccgccccg ctttacgtgg acataacgaa gacggtcatt aaagagggtg
      541 aagagcagct gcagactcag caccagaaaa ccttcatcgg gaaaatcccc attatgctgc
      601 gctccacgta ttgcttgttg aatggcttaa cagaccgtga cttgtgtgag ctgaatgagt
      661 gtcctttgga tcctggtggc tattttatta ttaatggatc agaaaaggtt ctgatagctc
      721 aagagaaaat ggcaacaaac acagtctatg tgtttgccaa aaaggattcc aagtatgcct
      781 atacaggaga atgccgctca tgcctagaga attcttcccg acccaccagt actatatggg
      841 ttagcatgct ggccagagga ggacagggtg caaagaagag tgcaattggc cagcgcatcg
      901 tggcgacatt accatatatc aagcaagaag ttcccatcat aattgtgttc agagcattag
      961 gctttgtgtc tgatagagat attttagagc acatcattta tgacttcgaa gacccagaga
     1021 tgatggaaat ggttaaacca tctctcgatg aagcatttgt catccaagag cagaatgttg
     1081 ccttaaactt cattggttca agaggggcga agcctggtgt tactaaggag aaaagaatta
     1141 aatatgcaaa agaagtctta cagaaagaga tgcttccgca tgtcggtgtc agtgatttct
     1201 gtgagaccaa aaaggcctat ttcttggggt acatggttca taggttactt ctagcagcct
     1261 tgggtagaag agaactagat gacagagatc actatggcaa caagagattg gatcttgctg
     1321 ggcctctgct tgcgttctta tttagaggca tgtttaagaa tttgctgaaa gaagtacgga
     1381 tctatgcaca gaagtttatt gaccgcggaa aggactttaa cctagagttg gcaattaaga
     1441 caaggatcat atccgatggg ctaaaatact ctttagccac tgggaactgg ggtgaccaga
     1501 agaaagctca ccaagccaga gctggagtgt cgcaggtact gaaccgcctg acttttgcgt
     1561 ctactctttc tcacctgcgt cgtttaaact ctcctattgg cagagatggc aagctagcaa
     1621 agccgagaca gttgcataat acattgtggg ggatggtgtg tcctgctgaa actccagagg
     1681 gccatgctgt aggacttgtg aagaatttag ccctgatggc ttatatttca gttggatctc
     1741 aaccttctcc aattttggaa tttttggaag aatggagcat ggaaaactta gaggaaattt
     1801 cccctgcagc tattgccgat gcaaccaaga tttttgttaa tggctgctgg gttggaatcc
     1861 ataaagaccc ggaacaactc atgaacacac tcaggaaatt acggcgtcag atggacatca
     1921 ttgtatccga agtttctatg atcagagata tccgcgagcg tgagattcgg atctatacag
     1981 acgcaggccg tatttgtaga ccacttctaa ttgtggaaaa gcaaaagctt cttttgaaga
     2041 agagacacat tgaccagttg aaagagagag aatataacaa ctacagttgg caggatctgg
     2101 tagccagtgg ggtagtagag tatattgata ccctggaaga agaaaccgtg atgcttgcaa
     2161 tgactccaga tgatttgcag gagaaagaag tagcttattg ctccacctat acccactgtg
     2221 agattcaccc atcaatgatt cttggcgtct gtgcatctat tattcctttt cctgatcata
     2281 accagtcccc tagaaacacg taccagtctg ctatgggcaa gcaggctatg ggagtttata
     2341 tcaccaactt ccacgttcgg atggacacct tggcgcacgt tctctactat cctcagaagc
     2401 cccttgtgac tacacggtct atggagtatc tccggtttag agaactgcca gcaggaatca
     2461 actcaattgt ggccattgca tcatacacgg gctataatca agaagactct gttatcatga
     2521 atcgttcagc tgtggatcga ggctttttca ggtctgtttt ctaccgatca tacaaagaac
     2581 aagagtctaa aaaaggattt gatcaagaag aagtgttcga gaagcctaca cgtgaaacat
     2641 gtcagggcat gaggcatgcc atttatgaca agttggacga tgatggcttg atagcgccag
     2701 gggtccgagt gtcgggagac gatgttatta taggcaaaac ggtcaccctc cctgagaacg
     2761 aggatgagct ggagagcacc aacaggcgct acaccaagag ggactgcagc accttcctca
     2821 gaaccagtga gacaggcatt gtggaccagg tcatggtcac tctcaaccag gaaggatata
     2881 aattttgtaa aataagggta cgctctgtta gaattccgca gatcggagac aaatttgcta
     2941 gccgacatgg tcagaagggt acttgtggta ttcagtatag acaagaggac atgccgttca
     3001 cttgtgaagg cataactcct gacatcatca taaaccccca cgccatcccc tctcggatga
     3061 cgattggtca cttgattgaa tgtctccaag ggaaggtctc tgcgaacaag ggtgaaattg
     3121 gtgatgctac tccgtttaat gatgctgtca atgtgcagaa gatttctaat cttttatctg
     3181 actacggcta ccatctcaga ggaaatgagg tgctctacaa tggattcact ggtcgcaaaa
     3241 tcacatcgca gatttttatt ggccccactt actaccagcg tttgaagcat atggtggatg
     3301 acaaaattca ttctcgtgct cgggggccca ttcagattct taacagacag cccatggagg
     3361 gcagatcgcg tgatggtggc ctgcgctttg gagaaatgga gcgagactgt cagatcgcac
     3421 atggtgcagc acagttctta agagaaaggc tgtttgaagc atcagatccg tatcaggttc
     3481 acgtttgtaa tctttgtgga ataatggcaa ttgctaacac caggactcac acatacgaat
     3541 gcaggggatg ccgcaataag acccagatct ccttggtgcg aatgccgtat gcgtgcaagc
     3601 tgttgttcca ggagctcatg tccatgagca tcgcaccacg gatgatgagt gtttagtggt
     3661 cagaagttca tggtccgata ctgtgagcag cagtatcttg tttcggtgac tgtcgttttt
     3721 aaaaaaaaaa atgacaaaat ttcctgttgt aataagaatt ttatttgttt tgtttaaagc
     3781 tctgcatgct ttttctgtaa ataaacaata aacttttgta gatagtctt
//
