LOCUS       NM_031773               3779 bp    mRNA    linear   ROD 29-MAR-2024
DEFINITION  Rattus norvegicus RNA polymerase I subunit B (Polr1b), mRNA.
ACCESSION   NM_031773
VERSION     NM_031773.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Vannini,A. and Cramer,P.
  TITLE     Conservation between the RNA polymerase I, II, and III
            transcription initiation machineries
  JOURNAL   Mol Cell 45 (4), 439-446 (2012)
   PUBMED   22365827
  REMARK    Review article
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Schneider,D.A.
  TITLE     RNA polymerase I activity is regulated at multiple steps in the
            transcription cycle: recent insights into factors that influence
            transcription elongation
  JOURNAL   Gene 493 (2), 176-184 (2012)
   PUBMED   21893173
  REMARK    Review article
REFERENCE   3  (bases 1 to 3779)
  AUTHORS   Chen,H., Li,Z., Haruna,K., Li,Z., Li,Z., Semba,K., Araki,M.,
            Yamamura,K. and Araki,K.
  TITLE     Early pre-implantation lethality in mice carrying truncated
            mutation in the RNA polymerase 1-2 gene
  JOURNAL   Biochem Biophys Res Commun 365 (4), 636-642 (2008)
   PUBMED   18023416
REFERENCE   4  (bases 1 to 3779)
  AUTHORS   Seither,P., Iben,S., Thiry,M. and Grummt,I.
  TITLE     PAF67, a novel protein that is associated with the
            initiation-competent form of RNA polymerase I
  JOURNAL   Biol Chem 382 (8), 1163-1170 (2001)
   PUBMED   11592397
REFERENCE   5  (bases 1 to 3779)
  AUTHORS   Hannan,R.D., Hempel,W.M., Cavanaugh,A., Arino,T., Dimitrov,S.I.,
            Moss,T. and Rothblum,L.
  TITLE     Affinity purification of mammalian RNA polymerase I. Identification
            of an associated kinase
  JOURNAL   J Biol Chem 273 (2), 1257-1267 (1998)
   PUBMED   9422795
REFERENCE   6  (bases 1 to 3779)
  AUTHORS   Seither,P., Coy,J.F., Pouska,A. and Grummt,I.
  TITLE     Molecular cloning and characterization of the cDNA encoding the
            largest subunit of mouse RNA polymerase I
  JOURNAL   Mol Gen Genet 255 (2), 180-186 (1997)
   PUBMED   9236775
REFERENCE   7  (bases 1 to 3779)
  AUTHORS   Seither,P. and Grummt,I.
  TITLE     Molecular cloning of RPA2, the gene encoding the second largest
            subunit of mouse RNA polymerase I
  JOURNAL   Genomics 37 (1), 135-139 (1996)
   PUBMED   8921381
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            On Nov 25, 2020 this sequence version replaced NM_031773.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF025424.1, SRR26643287.9112.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-237               JAXUCZ010000003.1  136787130-136787366
            238-405             JAXUCZ010000003.1  136789047-136789214
            406-552             JAXUCZ010000003.1  136789671-136789817
            553-685             JAXUCZ010000003.1  136791299-136791431
            686-822             JAXUCZ010000003.1  136794644-136794780
            823-1046            JAXUCZ010000003.1  136795755-136795978
            1047-1218           JAXUCZ010000003.1  136796565-136796736
            1219-1390           JAXUCZ010000003.1  136798748-136798919
            1391-1672           JAXUCZ010000003.1  136799635-136799916
            1673-1806           JAXUCZ010000003.1  136801771-136801904
            1807-1977           JAXUCZ010000003.1  136804015-136804185
            1978-2134           JAXUCZ010000003.1  136804917-136805073
            2135-2331           JAXUCZ010000003.1  136808303-136808499
            2332-2585           JAXUCZ010000003.1  136808931-136809184
            2586-3779           JAXUCZ010000003.1  136810415-136811608
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q36"
     gene            1..3779
                     /gene="Polr1b"
                     /gene_synonym="RPA2; Rpo1-2"
                     /note="RNA polymerase I subunit B"
                     /db_xref="GeneID:83582"
                     /db_xref="RGD:620822"
     exon            1..237
                     /gene="Polr1b"
                     /gene_synonym="RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             61..3468
                     /gene="Polr1b"
                     /gene_synonym="RPA2; Rpo1-2"
                     /EC_number="2.7.7.6"
                     /note="A127; RPA135; polymerase (RNA) I subunit B; RNA
                     polymerase I subunit 2; RNA polymerase I polypeptide B;
                     RNA polymerase I (127 kDa subunit); RNA polymerase 1-2;
                     polymerase (RNA) I polypeptide B"
                     /codon_start=1
                     /product="DNA-directed RNA polymerase I subunit RPA2"
                     /protein_id="NP_113961.2"
                     /db_xref="GeneID:83582"
                     /db_xref="RGD:620822"
                     /translation="MDVDSRWRNLPSGPSLKHLTDPSYAVPPEQQKAALQDLTRAHVD
                     SFNYAVLEGLSHAVQAIPPFEFAFKDERISLTIVDAAISPPAVPKGTICKELNIYPAE
                     CRGRRSTYRGKLTADISWAVNGVPKGIIKQFLGYVPIMVKSKLCNLYNLPPQVLIEHH
                     EEAEEMGGYFIINGIEKVIRMLIMPRRNFPIAMIRPKWKSRGLGYTQFGVSIHCVREE
                     HSAVNMNLHYVENGTVMLNFIYRKELFFLPLGFALKALVSFSDYQIFQELIKGKEEDS
                     FFKNSVSQMLRIVMEEGCHTQKQVLDYLGERFRVKLSLPDWYPNAEAAEFLFNQCICI
                     HLKSNTDKFYLLCLMTRKLFALARGECMEDNPDSLVNQEVLTPGQLFLMFLKEKMENW
                     LLSIKIALDKRAQKTNVSINNENLMKIFSMGTELTRPFEYLLATGNLRSKTGLGFMQD
                     SGLCVVADKLNFIRYLSHFRCVHRGADFAKMRTTTVRKLLPESWGFLCPVHTPDGAPC
                     GLLNHLTAVCEVVTKFVYTASIPALLCGLGVTPVDAAPCRPYSDCYPVLLDGVMVGWV
                     DKELAPEVADTLRRFKVLRERRVPPWMEVALIPMTGKPSLYPGLFLFTTPCRLVRPVQ
                     NLELGKEELVGTMEQLFMNIAIFEDEVFGGVSTHQELFPHSLLSVIANFIPFSDHNQS
                     PRNMYQCQMGKQTMGFPLLTYQDRSDNKLYRLQTPQSPLVRPCMYDHYDMDNYPIGTN
                     AIVAVISYTGYDMEDAMIVNKASWERGFAHGSVYKSEFIDLSEKFKQGDDSLVFGVKP
                     GDPRVMQKLDNDGLPFIGAKLEFGDPYYGYLNLNTGEGFVVYYKSKENCVVDNIKVCS
                     NDTGSGKFKCVCVTVRVPRNPTIGDKFASRHGQKGILSRLWPAEDMPFTESGMMPDIL
                     FNPHGFPSRMTIGMLIESMAGKSAALHGLCHDATPFIFSEENSALEYFGEMLKAAGYN
                     FYGTERLYSGISGMELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDKVTNQPIGGRN
                     VQGGIRFGEMERDALLAHGTSFLLHDRLFNCSDRSVAHVCVKCGSLLSPLLEKPPPSW
                     SAMRNRKYNCTVCGRSDSIDTVSVPYVFRYFVAELAAMNIKVKLDVI"
     exon            238..405
                     /gene="Polr1b"
                     /gene_synonym="RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     exon            406..552
                     /gene="Polr1b"
                     /gene_synonym="RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     exon            553..685
                     /gene="Polr1b"
                     /gene_synonym="RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     exon            686..822
                     /gene="Polr1b"
                     /gene_synonym="RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     exon            823..1046
                     /gene="Polr1b"
                     /gene_synonym="RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1047..1218
                     /gene="Polr1b"
                     /gene_synonym="RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1219..1390
                     /gene="Polr1b"
                     /gene_synonym="RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1391..1672
                     /gene="Polr1b"
                     /gene_synonym="RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1673..1806
                     /gene="Polr1b"
                     /gene_synonym="RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1807..1977
                     /gene="Polr1b"
                     /gene_synonym="RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1978..2134
                     /gene="Polr1b"
                     /gene_synonym="RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2135..2331
                     /gene="Polr1b"
                     /gene_synonym="RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2332..2585
                     /gene="Polr1b"
                     /gene_synonym="RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2586..3779
                     /gene="Polr1b"
                     /gene_synonym="RPA2; Rpo1-2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 attcctgcgg tccacgagtg gagtcgtagc tccggtttcc cgtccggggc tcgcagaagc
       61 atggatgtcg acagccggtg gcggaacctg cccagcgggc ccagcctaaa gcatttaacc
      121 gacccctcgt acgcggttcc tccggagcag caaaaggcgg cgttgcagga cctgacgcgg
      181 gcgcacgtgg actccttcaa ctacgcagtg ctggaggggc tgagccacgc ggtgcaggcc
      241 atacctccct tcgaatttgc tttcaaagat gagcgcatat ctcttactat tgtggacgct
      301 gccatcagtc cacccgcagt gcccaaaggg accatctgca aagagctcaa catttatcca
      361 gctgagtgcc gtggccggag gagcacgtac cgagggaagc tgacggctga tatcagctgg
      421 gccgtgaatg gagtccccaa aggcatcatt aaacaatttc ttggctatgt tcccatcatg
      481 gtgaagtcca agctttgcaa cttatacaac cttcctcctc aagtcctcat tgagcaccac
      541 gaggaggcag aggaaatggg cggttatttt ataatcaacg gcattgagaa agtcatccgc
      601 atgttgatta tgcctcggag aaattttccc atcgcaatga taagaccgaa atggaaaagc
      661 agagggctcg gctacactca gttcggggtt tccattcact gtgtgagaga ggagcactct
      721 gctgtcaata tgaaccttca ctatgtggag aacggcacgg tcatgttaaa ctttatttac
      781 cgcaaagagc tgtttttcct tcctttggga tttgcactta aggcacttgt gagcttttct
      841 gactatcaga ttttccagga gctcatcaaa ggcaaagagg aggactcttt ctttaagaat
      901 tctgtttctc agatgctgag gattgtaatg gaggagggtt gtcacacaca gaagcaggtc
      961 ctcgactatc tgggcgaacg cttcagagta aagctcagtc ttcccgattg gtaccctaat
     1021 gcggaagctg ccgagttcct gtttaaccag tgcatctgca tccacttgaa atccaacact
     1081 gacaagtttt acctgctctg tctcatgacc cggaagctct ttgctttagc cagaggagag
     1141 tgcatggagg acaatcctga cagtttagtg aatcaagaag tccttacccc tgggcagctc
     1201 ttcctgatgt ttctgaagga aaagatggag aattggctac tgtctattaa aatagcttta
     1261 gataaaaggg ctcagaagac caatgtttcc ataaacaatg aaaatttgat gaagattttt
     1321 agtatgggaa cagagctaac aagaccattt gaatatcttc ttgctactgg aaatctgcgt
     1381 tctaaaacag gtcttggctt catgcaggat tctggcctgt gtgttgtggc tgacaagctg
     1441 aacttcattc gctatctctc ccatttccgc tgtgtgcaca gaggggctga ctttgccaag
     1501 atgaggacca ccaccgtgcg caagctgctg ccagaatcct ggggcttcct ctgccctgtg
     1561 cacaccccag acggggcacc gtgtgggctg ctgaaccacc tgactgctgt gtgtgaggtt
     1621 gttaccaagt ttgtgtacac agcatctatt ccagccttgc tctgtggctt aggagtcact
     1681 cctgttgatg cagcaccatg tcgaccgtat agtgactgct accctgtcct gctggatggc
     1741 gtcatggtgg gctgggtgga taaggagctg gctcctgaag tggcagacac tctccgtcga
     1801 tttaaggtgt tgagagaaag gagagttcct ccctggatgg aggtggccct gattcccatg
     1861 acaggaaagc caagcctgta cccagggctg ttcctcttca ccactccctg caggctggtg
     1921 aggcctgtgc agaacctgga gctgggcaaa gaagagctcg ttggaactat ggagcagctc
     1981 ttcatgaaca ttgccatctt cgaggacgag gtttttggtg gagtttccac acaccaggag
     2041 ctcttccctc acagcctgct gagcgtgata gccaacttca tccccttctc tgatcacaac
     2101 cagagtcctc ggaacatgta ccagtgccag atgggtaagc agaccatggg cttcccgctg
     2161 ctcacctacc aagaccgatc agataataaa ctctatcgtc tccagacacc ccagagccct
     2221 ctagtgagac cgtgcatgta tgatcattat gacatggaca actatcccat cgggacaaac
     2281 gccattgtgg ctgtgatctc ctacactggc tatgatatgg aggacgccat gattgtaaac
     2341 aaggcctcct gggaacgagg ctttgctcat ggaagtgtct acaagtctga gttcatagac
     2401 ctctctgaga aatttaagca aggggatgat agtctggtat ttggggtcaa acctggtgac
     2461 ccacgggtta tgcagaagct ggacaatgat ggcttgccat tcataggagc aaagctggag
     2521 tttggtgatc cttactacgg ctacctaaac cttaacaccg gagaaggctt cgtggtttac
     2581 tataagagta aagaaaactg tgttgtggac aacatcaaag tgtgcagtaa tgacacagga
     2641 agtgggaagt tcaagtgcgt ctgcgtcacc gtccgagtcc cccggaaccc aactattgga
     2701 gataagtttg ccagccgcca cggacagaag ggcattttga gcagattgtg gccagctgag
     2761 gacatgcctt tcacagagag tgggatgatg ccggacattc tgtttaatcc tcatgggttt
     2821 ccctcccgta tgaccatagg tatgttaatc gagagtatgg ctgggaagtc agcagctttg
     2881 catggtctct gccatgatgc aacacccttc atcttctccg aggagaactc tgccctagag
     2941 tactttggtg agatgttaaa ggctgccggc tacaacttct atggcacgga gagattgtac
     3001 agcggcatca gcgggatgga gctggaggct gacattttca ttggtgtggt ttattaccag
     3061 cgcctacgac acatggtgtc agacaaattt caagtcagaa caactggagc cagggacaaa
     3121 gtcaccaacc agcccatcgg aggcaggaac gtccagggtg ggatccgatt tggggagatg
     3181 gagcgggatg ctctgttggc gcacggcaca tctttccttc tgcatgaccg cctcttcaac
     3241 tgctccgacc gctctgtggc ccacgtatgc gtgaagtgtg gcagtttgct ttctccgctg
     3301 ctcgagaagc ctcccccatc ttggtctgcg atgcgtaaca gaaaatacaa ctgcaccgtc
     3361 tgtggccgca gtgactccat cgacactgtc tctgtgccgt atgttttccg gtactttgta
     3421 gctgagctgg ctgccatgaa catcaaagtg aaactggacg tcatttaact tgatcacggc
     3481 catctgcgct aggagaagag aacaaaaggt gcctttaatc cagtgaggat actatgggtc
     3541 tgctctgggt ctatataaga atttcagtac ggaaatgtct cagtaaccta ctgaagttgg
     3601 ttttggtaca ttcattttta aaaaaaaaat tatgtgcctt ctttaaaaaa tgacttaatt
     3661 gataataggt catgcagggc ccttctgggc ccaggttcac tcgctgttcc ctgctttgag
     3721 tagtagagtg tgtccgccgt ctagagcagg gcagtacaat aaacagaaaa tggaagcca
//
