LOCUS       NP_113961               1135 aa            linear   ROD 29-MAR-2024
DEFINITION  DNA-directed RNA polymerase I subunit RPA2 [Rattus norvegicus].
ACCESSION   NP_113961
VERSION     NP_113961.2
DBSOURCE    REFSEQ: accession NM_031773.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 1135)
  AUTHORS   Vannini,A. and Cramer,P.
  TITLE     Conservation between the RNA polymerase I, II, and III
            transcription initiation machineries
  JOURNAL   Mol Cell 45 (4), 439-446 (2012)
   PUBMED   22365827
  REMARK    Review article
REFERENCE   2  (residues 1 to 1135)
  AUTHORS   Schneider,D.A.
  TITLE     RNA polymerase I activity is regulated at multiple steps in the
            transcription cycle: recent insights into factors that influence
            transcription elongation
  JOURNAL   Gene 493 (2), 176-184 (2012)
   PUBMED   21893173
  REMARK    Review article
REFERENCE   3  (residues 1 to 1135)
  AUTHORS   Chen,H., Li,Z., Haruna,K., Li,Z., Li,Z., Semba,K., Araki,M.,
            Yamamura,K. and Araki,K.
  TITLE     Early pre-implantation lethality in mice carrying truncated
            mutation in the RNA polymerase 1-2 gene
  JOURNAL   Biochem Biophys Res Commun 365 (4), 636-642 (2008)
   PUBMED   18023416
REFERENCE   4  (residues 1 to 1135)
  AUTHORS   Seither,P., Iben,S., Thiry,M. and Grummt,I.
  TITLE     PAF67, a novel protein that is associated with the
            initiation-competent form of RNA polymerase I
  JOURNAL   Biol Chem 382 (8), 1163-1170 (2001)
   PUBMED   11592397
REFERENCE   5  (residues 1 to 1135)
  AUTHORS   Hannan,R.D., Hempel,W.M., Cavanaugh,A., Arino,T., Dimitrov,S.I.,
            Moss,T. and Rothblum,L.
  TITLE     Affinity purification of mammalian RNA polymerase I. Identification
            of an associated kinase
  JOURNAL   J Biol Chem 273 (2), 1257-1267 (1998)
   PUBMED   9422795
REFERENCE   6  (residues 1 to 1135)
  AUTHORS   Seither,P., Coy,J.F., Pouska,A. and Grummt,I.
  TITLE     Molecular cloning and characterization of the cDNA encoding the
            largest subunit of mouse RNA polymerase I
  JOURNAL   Mol Gen Genet 255 (2), 180-186 (1997)
   PUBMED   9236775
REFERENCE   7  (residues 1 to 1135)
  AUTHORS   Seither,P. and Grummt,I.
  TITLE     Molecular cloning of RPA2, the gene encoding the second largest
            subunit of mouse RNA polymerase I
  JOURNAL   Genomics 37 (1), 135-139 (1996)
   PUBMED   8921381
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            On Nov 25, 2020 this sequence version replaced NP_113961.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF025424.1, SRR26643287.9112.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Rattus norvegicus"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q36"
     Protein         1..1135
                     /product="DNA-directed RNA polymerase I subunit RPA2"
                     /EC_number="2.7.7.6"
                     /note="A127; RPA135; polymerase (RNA) I subunit B; RNA
                     polymerase I subunit 2; RNA polymerase I polypeptide B;
                     RNA polymerase I (127 kDa subunit); RNA polymerase 1-2;
                     polymerase (RNA) I polypeptide B"
                     /calculated_mol_wt=127987
     Region          38..1133
                     /region_name="RNA_pol_B_RPB2"
                     /note="RNA polymerase beta subunit. RNA polymerases
                     catalyse the DNA dependent polymerization of RNA.
                     Prokaryotes contain a single RNA polymerase compared to
                     three in eukaryotes (not including mitochondrial. and
                     chloroplast polymerases). Each RNA polymerase...; cd00653"
                     /db_xref="CDD:238353"
     Site            order(106,770,808,810,814..816,818)
                     /site_type="other"
                     /note="RPB12 interaction site [polypeptide binding]"
                     /db_xref="CDD:238353"
     Region          186..375
                     /region_name="RNA_pol_Rpb2_2"
                     /note="RNA polymerase Rpb2, domain 2; pfam04561"
                     /db_xref="CDD:398318"
     Site            order(485,487..489,491,494,499,660,678..680,682,745..748,
                     751,753..755,890,892,894,916,918,921,924,929,933,950,981,
                     989..991,993,1006,1009..1011,1013..1014,1035..1037,1041,
                     1043..1044,1046,1048..1053,1055..1060,1064,1113,1117,1119,
                     1121..1122,1124,1126..1129,1131,1133)
                     /site_type="other"
                     /note="RPB1 interaction site [polypeptide binding]"
                     /db_xref="CDD:238353"
     Region          563..621
                     /region_name="RNA_pol_Rpa2_4"
                     /note="RNA polymerase I, Rpa2 specific domain; pfam06883"
                     /db_xref="CDD:462028"
     Site            order(718..719,740,742,763,766,899,912,939..940,942,977,
                     994)
                     /site_type="other"
                     /note="RPB10 interaction site [polypeptide binding]"
                     /db_xref="CDD:238353"
     Site            order(751,765,898,983)
                     /site_type="other"
                     /note="RPB11 interaction site [polypeptide binding]"
                     /db_xref="CDD:238353"
     Site            order(765..766,770,872,874,899..901,904,906..907,982..989,
                     991)
                     /site_type="other"
                     /note="RPB3 interaction site [polypeptide binding]"
                     /db_xref="CDD:238353"
     CDS             1..1135
                     /gene="Polr1b"
                     /gene_synonym="RPA2; Rpo1-2"
                     /coded_by="NM_031773.2:61..3468"
                     /db_xref="GeneID:83582"
                     /db_xref="RGD:620822"
ORIGIN      
        1 mdvdsrwrnl psgpslkhlt dpsyavppeq qkaalqdltr ahvdsfnyav leglshavqa
       61 ippfefafkd erisltivda aisppavpkg tickelniyp aecrgrrsty rgkltadisw
      121 avngvpkgii kqflgyvpim vksklcnlyn lppqvliehh eeaeemggyf iingiekvir
      181 mlimprrnfp iamirpkwks rglgytqfgv sihcvreehs avnmnlhyve ngtvmlnfiy
      241 rkelfflplg falkalvsfs dyqifqelik gkeedsffkn svsqmlrivm eegchtqkqv
      301 ldylgerfrv klslpdwypn aeaaeflfnq cicihlksnt dkfyllclmt rklfalarge
      361 cmednpdslv nqevltpgql flmflkekme nwllsikial dkraqktnvs innenlmkif
      421 smgteltrpf eyllatgnlr sktglgfmqd sglcvvadkl nfirylshfr cvhrgadfak
      481 mrtttvrkll peswgflcpv htpdgapcgl lnhltavcev vtkfvytasi pallcglgvt
      541 pvdaapcrpy sdcypvlldg vmvgwvdkel apevadtlrr fkvlrerrvp pwmevalipm
      601 tgkpslypgl flfttpcrlv rpvqnlelgk eelvgtmeql fmniaifede vfggvsthqe
      661 lfphsllsvi anfipfsdhn qsprnmyqcq mgkqtmgfpl ltyqdrsdnk lyrlqtpqsp
      721 lvrpcmydhy dmdnypigtn aivavisytg ydmedamivn kaswergfah gsvyksefid
      781 lsekfkqgdd slvfgvkpgd prvmqkldnd glpfigakle fgdpyygyln lntgegfvvy
      841 ykskencvvd nikvcsndtg sgkfkcvcvt vrvprnptig dkfasrhgqk gilsrlwpae
      901 dmpftesgmm pdilfnphgf psrmtigmli esmagksaal hglchdatpf ifseensale
      961 yfgemlkaag ynfygterly sgisgmelea difigvvyyq rlrhmvsdkf qvrttgardk
     1021 vtnqpiggrn vqggirfgem erdallahgt sfllhdrlfn csdrsvahvc vkcgsllspl
     1081 lekpppswsa mrnrkynctv cgrsdsidtv svpyvfryfv aelaamnikv kldvi
//
