LOCUS       NM_001105878            8527 bp    mRNA    linear   ROD 26-FEB-2024
DEFINITION  Rattus norvegicus DNA polymerase theta (Polq), mRNA.
ACCESSION   NM_001105878 XM_001062099 XM_001062161 XM_001062220 XM_001062278
            XM_001062339 XM_221423
VERSION     NM_001105878.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 8527)
  AUTHORS   Newman JA, Cooper CDO, Aitkenhead H and Gileadi O.
  TITLE     Structure of the Helicase Domain of DNA Polymerase Theta Reveals a
            Possible Role in the Microhomology-Mediated End-Joining Pathway
  JOURNAL   Structure 23 (12), 2319-2330 (2015)
   PUBMED   26636256
REFERENCE   2  (bases 1 to 8527)
  AUTHORS   Kent T, Chandramouly G, McDevitt SM, Ozdemir AY and Pomerantz RT.
  TITLE     Mechanism of microhomology-mediated end-joining promoted by human
            DNA polymerase theta;
  JOURNAL   Nat Struct Mol Biol 22 (3), 230-237 (2015)
   PUBMED   25643323
REFERENCE   3  (bases 1 to 8527)
  AUTHORS   Ceccaldi R, Liu JC, Amunugama R, Hajdu I, Primack B, Petalcorin MI,
            O'Connor KW, Konstantinopoulos PA, Elledge SJ, Boulton SJ, Yusufzai
            T and D'Andrea AD.
  TITLE     Homologous-recombination-deficient tumours are dependent on
            Poltheta;-mediated repair
  JOURNAL   Nature 518 (7538), 258-262 (2015)
   PUBMED   25642963
REFERENCE   4  (bases 1 to 8527)
  AUTHORS   Mateos-Gomez PA, Gong F, Nair N, Miller KM, Lazzerini-Denchi E and
            Sfeir A.
  TITLE     Mammalian polymerase theta; promotes alternative NHEJ and
            suppresses recombination
  JOURNAL   Nature 518 (7538), 254-257 (2015)
   PUBMED   25642960
REFERENCE   5  (bases 1 to 8527)
  AUTHORS   Hogg M, Sauer-Eriksson AE and Johansson E.
  TITLE     Promiscuous DNA synthesis by human DNA polymerase theta;
  JOURNAL   Nucleic Acids Res 40 (6), 2611-2622 (2012)
   PUBMED   22135286
REFERENCE   6  (bases 1 to 8527)
  AUTHORS   Zan H, Shima N, Xu Z, Al-Qahtani A, Evinger Iii AJ, Zhong Y,
            Schimenti JC and Casali P.
  TITLE     The translesion DNA polymerase theta plays a dominant role in
            immunoglobulin gene somatic hypermutation
  JOURNAL   EMBO J 24 (21), 3757-3769 (2005)
   PUBMED   16222339
REFERENCE   7  (bases 1 to 8527)
  AUTHORS   Masuda K, Ouchida R, Takeuchi A, Saito T, Koseki H, Kawamura K,
            Tagawa M, Tokuhisa T, Azuma T and O-Wang J.
  TITLE     DNA polymerase theta contributes to the generation of C/G mutations
            during somatic hypermutation of Ig genes
  JOURNAL   Proc Natl Acad Sci U S A 102 (39), 13986-13991 (2005)
   PUBMED   16172387
REFERENCE   8  (bases 1 to 8527)
  AUTHORS   Shima N, Munroe RJ and Schimenti JC.
  TITLE     The mouse genomic instability mutation chaos1 is an allele of Polq
            that exhibits genetic interaction with Atm
  JOURNAL   Mol Cell Biol 24 (23), 10381-10389 (2004)
   PUBMED   15542845
REFERENCE   9  (bases 1 to 8527)
  AUTHORS   Seki M, Marini F and Wood RD.
  TITLE     POLQ (Pol theta), a DNA polymerase and DNA-dependent ATPase in
            human cells
  JOURNAL   Nucleic Acids Res 31 (21), 6117-6126 (2003)
   PUBMED   14576298
REFERENCE   10 (bases 1 to 8527)
  AUTHORS   Shima N, Hartford SA, Duffy T, Wilson LA, Schimenti KJ and
            Schimenti JC.
  TITLE     Phenotype-based identification of mouse chromosome instability
            mutants
  JOURNAL   Genetics 163 (3), 1031-1040 (2003)
   PUBMED   12663541
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH473967.1.
            
            On or before Oct 3, 2007 this sequence version replaced
            XM_221423.4, XM_001062099.1, XM_001062278.1, XM_001062339.1,
            XM_001062161.1, XM_001062220.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMD00132261, SAMD00132262
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..8527
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="11"
                     /map="11q21"
     gene            1..8527
                     /gene="Polq"
                     /note="DNA polymerase theta"
                     /db_xref="GeneID:288079"
                     /db_xref="RGD:1309795"
     exon            1..279
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     CDS             114..7757
                     /gene="Polq"
                     /EC_number="2.7.7.7"
                     /note="polymerase (DNA directed), theta; polymerase (DNA)
                     theta"
                     /codon_start=1
                     /product="DNA polymerase theta"
                     /protein_id="NP_001099348.1"
                     /db_xref="GeneID:288079"
                     /db_xref="RGD:1309795"
                     /translation="MSLPRRSGKRRRSSSGSDSFSFSGDGDSCVSPQLLCRPVLSPPP
                     GLGRGRRLAGTGTCKQRVSDDQIDQLLLANWGLPKAVLEKYHNFGVKKMFEWQAECLL
                     LGQVLEGKNLVYSAPTSAGKTLVAELLILKRVLETRKKALFILPFVSVAKEKKYYLQS
                     LFQEVGIKVDGYMGSTSPTGRFSSLDVAVCTIERANGLINRLIEENKMDLLGTVVVDE
                     LHMLGDSHRGYLLELLLTKVCFVTRKSASCQADSASALACAVQIVGMSATLPNLQLVA
                     SWLNAELYHTDFRPVPLLESIKVGNSIYDSSMKLVREFQPLLQVKGDEDHIVSLCYET
                     VRDNHSVLVFCPSKKWCEKVADIIAREFYNLHHQPEGLVKSSEFPPVILDQKSLLEVI
                     DQLKRSPSGLDSVLKNTVPWGVAFHHAGLTFEERDIIEGAFRQGLIRVLAATSTLSSG
                     VNLPARRVIIRTPVFGGQPLDILTYKQMVGRAGRKGVDTMGESILVCKNSEKSKGIAL
                     LQGSLEPVHSCLQSQGEVTSTMIRAILEIIVSGVASTSQDMQTYAACTFLAADVKEGK
                     QGIQRNRDDVQRGAVDACVTWLLENEFIQAAEPSDGTGGKVYHPTHLGSATLSSSLSP
                     TDTLDIFADLQRAMKGFVLENDLHIVYLVTPVFEDWTSIDWYRFFCLWEKLPTSMKRV
                     AELVGVEEGFLARCVKGKVVARTERQHRQMAIHKRFFTSLVLLDLISEIPLKEINQKY
                     GCNRGQIQSLQQSAAVYAGMITVFSNRLGWHNMELLLSQFQKRLTFGIQRELCDLIRV
                     SSLNAQRARFLYASGFLTVADLARADTVEVEAALKDALPFKSARKAVDEEEEAAEERR
                     SMRTIWVAGKSLSAREAAALIVEEAKVILQQDLIEMGVQWGPHSPLSSSTHSLTSGSE
                     VKEHTFKSQTKSSHKRLASKSRNSMRVSGSNGKQSPEAGQGLDECRERPDSLCKFQGN
                     HEIQTPSVYRARKRTSLGVNKEMLRTSLKEGKPSTKEVLQTLSFEKTRKAALSFSSEQ
                     ANNSFPSGRDRKYRKKSWGSSPMSDSVMHRDDLQGQTMCKSTLCEDPQKSLEEQNTEY
                     RSPGLFAKNVSFCAKEKCNKTSFPLQMQQPCLRRKPESGAAVDHSVAVSQNKNVVEQP
                     PGAPRDRRGLAAHGRAEVNEVLTENGTESQLHDTHPVSQCLENHSEKQTNTCTRQKTL
                     TEGQAGISHVTRGSNDLTPIRCERLKLNSKEHDSNPCPQALGTNAGRTEAPQSSEALG
                     QAGGQCENLLNSPGIQEKTSAHATNKTEHSHVANQAFCDFGDSLYLDTQSEEIIEQMA
                     TKNATQGAEAAGITEEGSATQNEPHSTTGGQHIPGAANTDHVDRKNTESVKENPEKNI
                     DRRTPHSLIFHSPTPQGGNSACFKENEHSVTDSQLNSFLQGLETQDKPIIPLAPQMRT
                     STGVEEESLPETSLNMSDSILFDSFGEDSFGQRQSLDVKAKQPLLSEMTPNHFHNPPY
                     PQEDPVMTPHMSEPQGTLERMACLSGESIIFSEIDSAQVIEALDNMAAFYMQENCNPI
                     TLKTEPRDLAALGNECPQGEVVRGEQHEGSSKPKFMEINQDNSFTWSAASFNLSPELQ
                     RILDKVSTPRENEEPELMHADLSCFEENSTESHERQDMNSDLGTVQRTSFLPSNGVKS
                     RTEGLESKAKHGGASSALPHKAAADDNGLIPPTPLPASASASASKLALPEILGTSVKH
                     QKASCLFDSPSDNQNQDLSQELRDSLKDSDGSVVDTSFFLQSQDGLLLTQASCSSESL
                     AIIDVASDQILFQTFVKEWQCQKRFSISLACEKMTSSTSSKTATIGGRLKQVNSPQEA
                     SVEDDGFPVHGSDCAVVVGLAVCWGGKDAYYLSLQKEQKQSEMSPSLAPPPLDATLTV
                     KERMEYLQSCLQKKSDQERSVVTYDFIQTYKVLLLSCGISLEPSYEDPKVACWLLDPD
                     SKEPTLHSIVTSFLPHELALLEGIETGPGIQSLGLNVNTDHSGRYRASVESVLIFNSM
                     NQLNSMLQKENLHDIFCKVEMPSQYCLALLELNGIGFSTAECETQKHIMQAKLDAIET
                     QAYQLAGHSFSFTSADDIAQVLFLELKLPPNGEMKTQGGRKTLGSTRRGTESDRKLRL
                     GRRFSTSKDILNKLKDLHPLPGLILEWRRISNAITKVVFPLQREKHLNPFLRMERIYP
                     VSQSHTATGRITFTEPNIQNVPRDFEIKMPTLVRESPPSQASGKGQLAMARQNQKVYG
                     LHPGQRTVLEKTSDRGVPFSVSMRHAFVPFPGGLILAADYSQLELRILAHLSRDCRLI
                     QVLNSGADVFRSIAAEWKMIEPDAVGDNLRQQAKQICYGIIYGMGAKSLGEQMGIKEN
                     DAACYIDSFKSRYKGINHFMRDTVKNCRRDGFVETILGRRRYLPGIKDNNPYHKAHAE
                     RQAINTTVQGSAADIVKVATVNIQKQLETFHPTFKSHGHRESMLQSDRAGLLPKRKVK
                     GMFCPMRGGFFILQLHDELLYEVAEEDVVQVAQIVKNEMECAIKLSVKLKVKVKIGAS
                     WGELKDFDV"
     exon            280..459
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            460..590
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            591..747
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            748..856
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            857..1076
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..1224
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            1225..1371
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            1372..1584
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            1585..1721
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            1722..1926
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            1927..2069
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            2070..2263
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            2264..2388
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            2389..2632
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            2633..5619
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            5620..5763
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            5764..5960
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            5961..6201
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            6202..6395
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            6396..6533
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            6534..6708
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            6709..6835
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            6836..6951
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            6952..7136
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            7137..7248
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            7249..7373
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            7374..7527
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            7528..7643
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
     exon            7644..8527
                     /gene="Polq"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gaagcagagg cgggaagatg gtggcggtag ctgtcactag gctggggttc tgtcgcacgg
       61 gaggcctctg ggactgtgtc gtgtcctgaa cagttaagag gaacttgttt gcaatgagtc
      121 ttccgcgccg gagtgggaaa cggcggcgtt catcatccgg ctccgactca ttctcgttct
      181 cgggagacgg tgatagctgt gtcagccctc agctcctgtg taggccggtg ctgagtccac
      241 ctccggggct gggacgcggc cggaggctcg cagggacagg aacatgcaag caaagagttt
      301 ctgatgacca gatagaccag ctgctattgg caaactgggg actccctaaa gcagttctgg
      361 agaaatacca caattttggt gtaaaaaaga tgttcgaatg gcaggcagag tgtcttttgc
      421 tcggacaagt cctggaagga aagaatctgg tttattcagc tcctacaagt gctgggaaga
      481 cgcttgttgc agagttactt attttgaagc gggttttgga aacgcggaag aaagcgttgt
      541 tcattctgcc ctttgtgtct gtggctaaag agaagaaata ctatctccag agtctgtttc
      601 aggaagtagg aataaaggta gatggctata tgggcagcac ctcaccaact ggacgtttct
      661 cctccttgga cgttgctgtt tgcactattg agagagccaa tgggctgatc aatcgcctca
      721 ttgaggaaaa taagatggat ctcttaggaa cggtggtcgt ggatgagcta cacatgctgg
      781 gggactctca ccgagggtat ctgctggaac tcttactgac caaggtttgc tttgtcactc
      841 ggaagtcagc atcgtgccag gcagattcgg ccagtgctct ggcttgtgct gtgcagattg
      901 ttggcatgag tgctactctt cctaacctgc agctcgtggc ttcctggctg aatgctgaac
      961 tttaccatac tgactttcgc cctgttccac ttttggaatc aataaaagta ggaaattcca
     1021 tatatgactc ttcaatgaag cttgtgagag aatttcaacc cctgctccaa gtgaagggag
     1081 atgaagacca cattgttagt ttatgctatg agacggttcg ggataaccat tcagtattag
     1141 tcttttgtcc atcaaaaaaa tggtgtgaga aggtggccga tatcattgcc cgggagtttt
     1201 ataatctgca ccatcaaccc gagggattag taaagtcatc tgaatttcca ccagtgattc
     1261 tagaccaaaa gagccttctt gaagtgatag atcagttaaa acgctcacct tcaggactag
     1321 actcggtgct gaagaatact gtgccatggg gagtagcgtt tcatcatgca ggtcttactt
     1381 ttgaagagag ggatatcatc gaaggagcct tccgtcaagg tcttattcgc gttttggcag
     1441 caacgtctac tctttcttct ggagtgaacc ttcctgcacg tcgggtgatt attcgcactc
     1501 cggttttcgg tggtcagcct ttagatatcc tcacttataa gcagatggtt ggtcgtgctg
     1561 gcaggaaagg agtagacaca atgggtgaaa gtatactagt ttgtaagaac tctgagaaat
     1621 ccaagggcat tgctctgctt caaggttcgc tggagcctgt gcacagctgt ctgcaaagcc
     1681 agggagaagt cacttctacc atgatacgag ctattctgga gataatcgtc agtggggtgg
     1741 caagtacgtc acaagatatg cagacttatg ctgcctgcac attcttggct gctgacgtaa
     1801 aggaagggaa gcaagggatt cagaggaacc gagatgatgt tcagcgtggg gcagtcgatg
     1861 cctgtgtgac gtggctgtta gaaaacgagt tcatccaggc ggcagagccc agtgacggca
     1921 caggaggaaa ggtgtatcac ccaacacacc ttggatcagc tactctttct tcctcacttt
     1981 ctccaactga taccctggat atttttgctg acctccaaag agcaatgaaa ggctttgttt
     2041 tagagaatga ccttcatatc gtctacctgg ttacacctgt gtttgaagat tggactagta
     2101 ttgattggta tcggtttttc tgtttatggg aaaaattacc aacttctatg aaaagggtgg
     2161 cagagctggt tggagtggag gaagggttct tggctcgatg tgtgaaagga aaagtagtag
     2221 ccagaactga gagacagcat cgacaaatgg ccatccataa aaggtttttc acaagccttg
     2281 tgttattgga tttaatcagt gaaattccct taaaggaaat taaccagaaa tatggatgta
     2341 atcgtgggca gattcagtct ttgcaacagt cagctgctgt ctatgcagga atgattacgg
     2401 tgttttccaa ccgtctgggc tggcacaaca tggagctact gctttctcag tttcagaagc
     2461 gtcttacgtt tggcatccag agagagctgt gtgacctgat ccgggtatcc tcgctcaacg
     2521 ctcagagagc caggttcctg tatgcctctg gctttctcac cgtggcggac ctcgccagag
     2581 ctgacactgt agaggtggag gcggctctga aggacgctct gcctttcaaa agtgcccgga
     2641 aggcagtgga cgaagaagag gaagcagctg aagaacgccg gagtatgcgc actatttggg
     2701 tggctggcaa gagcttatct gcaagggaag cagcagcttt aatagtagaa gaagccaaag
     2761 tgattctgca gcaggactta attgaaatgg gagtacagtg gggtccacac tcacccttga
     2821 gttctagcac acactcactg accagtgggt cagaagtaaa ggagcacacg ttcaagtccc
     2881 agactaagag ttctcataaa aggttagcat caaagagcag gaacagcatg agagtgagtg
     2941 gttctaatgg taagcagtca ccagaggcag ggcaaggcct agacgagtgc agagagcgcc
     3001 cagactctct ctgtaagttc caggggaatc acgagatcca gacaccctcc gtttataggg
     3061 caagaaaacg gacttcttta ggtgtaaaca aagaaatgct tcgaacctct ctgaaggaag
     3121 gaaaaccaag cactaaggaa gttcttcaaa ctctctcatt tgagaaaaca agaaaagctg
     3181 ctttgagttt tagttcagag caggcgaaca acagttttcc atctgggaga gacagaaagt
     3241 atcggaaaaa atcttggggt agcagtccca tgagtgactc tgtgatgcat agagatgatt
     3301 tacaaggaca gactatgtgt aaatctactc tatgtgaaga cccacagaag tccttagagg
     3361 agcagaatac agaatacaga agtccagggc tatttgcaaa aaatgtatct ttttgtgcca
     3421 aagaaaaatg caacaaaacc tcattcccct tacaaatgca gcagccttgc ttaaggagaa
     3481 aaccagaaag tggtgctgcc gtggaccatt ccgttgcggt gtctcagaat aaaaatgtgg
     3541 tggagcaacc ccctggtgct cctagggaca gaagaggatt ggctgctcat ggcagagcag
     3601 aggtaaatga ggtgctcaca gagaatggta cagaaagcca actccacgac actcatcctg
     3661 tcagccagtg cctggaaaac cacagtgaga agcagacaaa cacgtgcacc agacagaaga
     3721 cattgacaga ggggcaagct ggtattagtc atgtgactag aggctcaaat gatctcacac
     3781 ccatcagatg tgaacgttta aagcttaaca gcaaagaaca tgactcaaat ccttgtcctc
     3841 aagcattggg aactaacgca ggcagaactg aggcacctca gtcatctgaa gcacttggtc
     3901 aagcaggagg ccagtgtgaa aatcttctaa attctcctgg tatacaagaa aaaacaagtg
     3961 ctcatgcaac aaataaaact gaacatagtc atgttgctaa ccaagccttc tgtgactttg
     4021 gagatagctt atacttagac actcagtcag aggaaataat tgaacagatg gcaactaaaa
     4081 atgccacgca aggagcagaa gcagcaggga taacggagga gggctccgcc actcagaacg
     4141 agcctcacag cactacaggt ggccagcata tcccaggagc agccaacaca gaccatgtgg
     4201 accgtaagaa cacagagtct gtgaaagaaa accccgaaaa gaacatcgac agaagaactc
     4261 cacacagcct cattttccat tctccaacac ctcagggggg aaacagtgct tgctttaaag
     4321 agaatgagca ttctgttact gattcccagt taaacagttt tcttcaaggg ttggaaacac
     4381 aggacaagcc aatcatacct ctggctcctc aaatgagaac ttctactggt gtagaggaag
     4441 aaagtctgcc tgaaaccagt ttgaatatga gtgacagtat actatttgac agttttggag
     4501 aagatagctt tggacaaaga cagtcacttg atgtaaaggc aaaacagccc ctcctttccg
     4561 aaatgacacc aaaccatttc cacaatcctc catacccaca agaagaccca gttatgacac
     4621 cacatatgag cgagcctcag ggtactctgg agcgaatggc ttgtctcagt ggtgaatcta
     4681 ttatattttc agagatagac tctgctcagg tgatcgaagc cttagacaat atggctgcat
     4741 tttacatgca agaaaactgt aacccaataa ctcttaaaac agaacccaga gatttggcag
     4801 cgctgggtaa tgagtgtccc caaggagaag tggtcagagg agaacaacat gaagggtcct
     4861 caaagcccaa attcatggag ataaatcaag ataattcatt cacttggtca gcagcatcat
     4921 ttaacttaag tccagagctg caaaggattt tagataaagt gtctactcct cgagaaaatg
     4981 aagagcccga attgatgcat gcagacttgt cttgctttga agaaaacagc acagagtcac
     5041 atgagagaca ggacatgaat tcagacttgg ggacagttca gagaacttca tttctcccca
     5101 gtaatggagt taaaagcagg actgaagggc tagagagcaa agccaagcat ggtggagcct
     5161 catctgcctt gcctcataaa gctgcagctg atgacaacgg cctcattcct cctacacccc
     5221 ttcctgcctc tgcctctgcc tctgcctcta agctagcact tccagagatt ctcggaacat
     5281 ctgtaaaaca tcagaaagcc agttgtttat ttgactcacc ttcagataat caaaaccagg
     5341 atttaagtca agagcttaga gacagcctca aggactctga tggttctgtt gtagacacaa
     5401 gtttttttct gcagtctcag gatggactgc tgttaactca agcctcatgc agctcagaga
     5461 gcctggccat aattgacgta gcgagtgacc agattctctt tcagacattt gtgaaggagt
     5521 ggcagtgcca aaagcgcttt tccatctctc tagcttgtga aaagatgaca agttcgacgt
     5581 cttccaaaac tgctaccata ggtggaaggt tgaagcaagt taactcacca caggaagcct
     5641 ctgttgaaga tgatgggttc cctgtgcatg gctctgactg tgctgtggta gttggactag
     5701 cagtgtgctg gggtggaaag gatgcctatt atttgtcatt gcagaaggaa caaaagcagt
     5761 ctgaaatgag tcctagtttg gccccaccac ctctggatgc aacactaact gttaaggaga
     5821 ggatggagta ccttcagtcc tgcttgcaaa agaagtctga ccaagaacgg tctgttgtca
     5881 cctatgattt catccagacc tataaagttc tcctcttgtc ctgtggcatc tcactggaac
     5941 ccagctatga agaccccaag gtggcttgct ggcttcttga cccggattct aaggagccaa
     6001 ctcttcatag catagtgacc agttttctcc cccatgagct agcactccta gaaggaatag
     6061 agacaggccc aggaattcag agcctggggc taaatgtgaa cacagaccat tctgggcggt
     6121 acagagcgag tgtggagtct gttctcatct tcaactccat gaatcaactc aactctatgt
     6181 tgcagaagga aaaccttcac gatattttct gcaaagtgga gatgccctct cagtactgcc
     6241 tggccttgct ggaactaaat ggaattggct ttagtactgc tgagtgtgaa actcagaagc
     6301 atatcatgca agccaagctg gacgccattg agactcaagc ctatcaactc gctggccaca
     6361 gcttctcctt tacaagtgca gatgacatcg cacaggtttt atttttggaa ctaaagctac
     6421 caccgaatgg agagatgaaa acacaaggcg gcagaaagac tctgggttcc accagaagag
     6481 ggacggagag cgaccgcaaa ctcaggctgg gaagacggtt cagcactagt aaggatattt
     6541 taaataagtt aaaggacttg catcctttac caggcctgat actggaatgg agaagaatca
     6601 gtaatgctat taccaaagtg gtcttcccct tgcagcggga aaagcacctg aacccctttc
     6661 tcagaatgga gaggatctat cctgtatcac agtcacacac ggctacagga cggataacct
     6721 ttacagaacc caatatccaa aatgtgccaa gagattttga gattaaaatg ccaacactag
     6781 taagagagag cccaccttct caagcttcag gcaagggcca gctcgcgatg gccagacaaa
     6841 atcagaaggt ttatggcctg cacccggggc aaagaacggt gctggagaag acctcagata
     6901 gaggcgtgcc attttcagtg agcatgcgcc atgcctttgt gcctttccca ggtggtttga
     6961 tattggctgc cgactactct cagctcgaac tgaggatcct ggcacactta tctcgtgact
     7021 gtcgtctcat tcaagtttta aactcaggag cggatgtgtt caggagcatt gccgctgagt
     7081 ggaagatgat tgagcccgat gctgttggtg acaatctgag gcaacaggca aagcagattt
     7141 gctatggaat tatatatggg atgggagcta aatcgttagg agagcagatg ggcattaaag
     7201 aaaatgatgc tgcttgctat atcgactctt tcaagtccag gtataaaggg attaatcatt
     7261 tcatgaggga cacagtgaag aactgtagaa gagatggatt tgttgagaca attttgggaa
     7321 gacgtagata tttgccagga atcaaagaca ataatcctta tcataaggca cacgctgagc
     7381 gtcaggccat caacacaaca gttcaaggct cagctgctga catagtcaaa gtagccacag
     7441 ttaacatcca gaagcagcta gagaccttcc atccaacctt caaatcccat ggtcatcggg
     7501 aaagcatgct ccagagtgac cgagcaggat tgttgccaaa gagaaaagtt aaagggatgt
     7561 tttgtcctat gagaggagga ttcttcatcc ttcaacttca cgacgagctt ttatatgaag
     7621 tggcagaaga ggatgttgtt caggtagctc agattgtcaa gaatgaaatg gaatgtgcta
     7681 ttaaactttc tgtgaagctg aaagtgaaag tgaaaatagg tgccagctgg ggggagctga
     7741 aggactttga tgtgtgatgg tgcagtgccc aggtcccagg gcagcctggg acacagtcac
     7801 tcagaaagca gagtctgccc tttcgcctgc tctcaagtac gatgcactta aacgagcagg
     7861 tctctagcgg gctttaatgt ctacctcttc tggtccctgt ggtgttacaa ggctttgagg
     7921 ggagatttat tcgttatcct cactctcaaa gagcatctat gaatttgtct ttcataattt
     7981 attggagcgt taaacagtct cagaatgtat gcttgaaaaa tgcacttagc actttgggtc
     8041 ctcaactgtg tagattgtga aggagtggag ctgctgcctc agggccattt ttattcctgt
     8101 caaggactcc ggcactcatg atgtaatccc tcaagagcct cgggcagtgc tcagtctcag
     8161 tcattagagc tatgcagtat gtgtggcctt ttcctacagt aaagcttctg aagtcgaatt
     8221 ttaaaatctc aaattgaaat tctataagat agttaatatg tgtggaagga atctagaaat
     8281 gagttttcat gaactgttgc tgcttgttct tttcctcacc gttccgacaa ggaaggattg
     8341 gaatgctctt aaaatgaact gtctgtcagc ttagacattg agacacttga atctaacact
     8401 gccaggccag aaaaatgccc ttttgcgagc cccttagtgc ctccatccgt atttgtggta
     8461 tggagagaat tctcaggctc aaaacattga atttctttac tcataactaa tatccttttg
     8521 ctgttca
//
