LOCUS       NM_001107060            1872 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus DNA polymerase gamma 2, accessory subunit
            (Polg2), mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001107060 XM_001081578 XM_221047
VERSION     NM_001107060.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Siibak,T., Clemente,P., Bratic,A., Bruhn,H., Kauppila,T.E.S.,
            Macao,B., Schober,F.A., Lesko,N., Wibom,R., Naess,K., Nennesmo,I.,
            Wedell,A., Peter,B., Freyer,C., Falkenberg,M. and Wredenberg,A.
  TITLE     A multi-systemic mitochondrial disorder due to a dominant p.Y955H
            disease variant in DNA polymerase gamma
  JOURNAL   Hum Mol Genet 26 (13), 2515-2525 (2017)
   PUBMED   28430993
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Ciesielski,G.L., Bermek,O., Rosado-Ruiz,F.A., Hovde,S.L.,
            Neitzke,O.J., Griffith,J.D. and Kaguni,L.S.
  TITLE     Mitochondrial Single-stranded DNA-binding Proteins Stimulate the
            Activity of DNA Polymerase gamma by Organization of the Template
            DNA
  JOURNAL   J Biol Chem 290 (48), 28697-28707 (2015)
   PUBMED   26446790
REFERENCE   3  (bases 1 to 1872)
  AUTHORS   Bratic,A., Kauppila,T.E., Macao,B., Gronke,S., Siibak,T.,
            Stewart,J.B., Baggio,F., Dols,J., Partridge,L., Falkenberg,M.,
            Wredenberg,A. and Larsson,N.G.
  TITLE     Complementation between polymerase- and exonuclease-deficient
            mitochondrial DNA polymerase mutants in genomically engineered
            flies
  JOURNAL   Nat Commun 6, 8808 (2015)
   PUBMED   26554610
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1872)
  AUTHORS   Young,M.J., Humble,M.M., DeBalsi,K.L., Sun,K.Y. and Copeland,W.C.
  TITLE     POLG2 disease variants: analyses reveal a dominant negative
            heterodimer, altered mitochondrial localization and impaired
            respiratory capacity
  JOURNAL   Hum Mol Genet 24 (18), 5184-5197 (2015)
   PUBMED   26123486
REFERENCE   5  (bases 1 to 1872)
  AUTHORS   Humble,M.M., Young,M.J., Foley,J.F., Pandiri,A.R., Travlos,G.S. and
            Copeland,W.C.
  TITLE     Polg2 is essential for mammalian embryogenesis and is required for
            mtDNA maintenance
  JOURNAL   Hum Mol Genet 22 (5), 1017-1025 (2013)
   PUBMED   23197651
REFERENCE   6  (bases 1 to 1872)
  AUTHORS   Korhonen,J.A., Pham,X.H., Pellegrini,M. and Falkenberg,M.
  TITLE     Reconstitution of a minimal mtDNA replisome in vitro
  JOURNAL   EMBO J 23 (12), 2423-2429 (2004)
   PUBMED   15167897
REFERENCE   7  (bases 1 to 1872)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   8  (bases 1 to 1872)
  AUTHORS   Carrodeguas,J.A., Theis,K., Bogenhagen,D.F. and Kisker,C.
  TITLE     Crystal structure and deletion analysis show that the accessory
            subunit of mammalian DNA polymerase gamma, Pol gamma B, functions
            as a homodimer
  JOURNAL   Mol Cell 7 (1), 43-54 (2001)
   PUBMED   11172710
REFERENCE   9  (bases 1 to 1872)
  AUTHORS   Carrodeguas,J.A. and Bogenhagen,D.F.
  TITLE     Protein sequences conserved in prokaryotic aminoacyl-tRNA
            synthetases are important for the activity of the processivity
            factor of human mitochondrial DNA polymerase
  JOURNAL   Nucleic Acids Res 28 (5), 1237-1244 (2000)
   PUBMED   10666468
REFERENCE   10 (bases 1 to 1872)
  AUTHORS   Lim,S.E., Longley,M.J. and Copeland,W.C.
  TITLE     The mitochondrial p55 accessory subunit of human DNA polymerase
            gamma enhances DNA binding, promotes processive DNA synthesis, and
            confers N-ethylmaleimide resistance
  JOURNAL   J Biol Chem 274 (53), 38197-38203 (1999)
   PUBMED   10608893
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH473948.1.
            
            On or before Oct 4, 2007 this sequence version replaced
            XM_221047.4, XM_001081578.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMD00132261, SAMD00132262
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q32.1"
     gene            1..1872
                     /gene="Polg2"
                     /note="DNA polymerase gamma 2, accessory subunit"
                     /db_xref="GeneID:303612"
                     /db_xref="RGD:1305128"
     exon            1..873
                     /gene="Polg2"
                     /inference="alignment:Splign:2.1.0"
     CDS             390..1769
                     /gene="Polg2"
                     /EC_number="2.7.7.7"
                     /note="polymerase (DNA directed), gamma 2, accessory
                     subunit; polymerase (DNA) gamma 2, accessory subunit"
                     /codon_start=1
                     /product="DNA polymerase subunit gamma-2, mitochondrial"
                     /protein_id="NP_001100530.1"
                     /db_xref="GeneID:303612"
                     /db_xref="RGD:1305128"
                     /translation="MHSGGVARACRRACRCWLSGYAGPTDGTQQPTDPEHALAREALV
                     DLCRRRHFLSGTPQQLSTAALLSGCHSGFGPLGVELRKNLASQWWSSMVVFREQVFAV
                     DSLHHEPGSSQPRDSAFRLVSPECIREILQDKELSKEQLVAFLENLLKTSGKLRETLL
                     HGALEHYVHCLDLVNRKLPYGLAQIGVCFHPVLNPKEIPNSVTRVGEKTEASLVWFTP
                     TRTSSQWLDFWLRHRLLWWRKFAVSPSNFSSVDCQDGSGRKGCRLYYSFPWGKEPLET
                     LWDLGEQELLNVYPGDVSAIQGRDGRKNVVPSVLSVNGDLDLGTLAYLYDSFQFTENS
                     FSRKKSLQRKVLKLHPCLAPIKVALDVGKGPTVELRQVCQGLLNELLENGIAVWPGYL
                     ETAQSSLEQLYSKYDEMSILFTVLITETTLESGLIQLRSRDTTMKEMMHISRLRDFLV
                     KYLASAGKA"
     exon            874..1000
                     /gene="Polg2"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..1106
                     /gene="Polg2"
                     /inference="alignment:Splign:2.1.0"
     exon            1107..1280
                     /gene="Polg2"
                     /inference="alignment:Splign:2.1.0"
     exon            1281..1421
                     /gene="Polg2"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..1502
                     /gene="Polg2"
                     /inference="alignment:Splign:2.1.0"
     exon            1503..1603
                     /gene="Polg2"
                     /inference="alignment:Splign:2.1.0"
     exon            1604..1872
                     /gene="Polg2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ttcccttgga gactgaacac acgctttagc ctcacctcat cgccatccag gacaattttg
       61 acaaggcgtg tttttgtttt cgcatctcca aaaaacatgc taattccata gtaacaggat
      121 ggtggtaaaa gctaacaagt gaatgtgagg caggtggagt taccaaacgg aaaatcccca
      181 aggtagtaaa caaccacaaa cgctggcgcc agccgcggag gccgggatac gcccaaggcg
      241 gctgcggccc agcagcgggt gatgctcttc cgggggcgtg gtctgcggcg cgttcatttc
      301 cgccgcgcct gcgccctagg cttttggtgc tggtttagga tagagacggc ttcccacgtg
      361 aggcgccggt gttcccgagt ccggaagcga tgcactcggg tggcgtggcc agggcctgcc
      421 gcagggcctg caggtgctgg ctgtccggat atgcgggtcc cacagatggg acgcagcagc
      481 ctacggaccc cgagcatgca ttggcccgcg aggcgctggt agacctctgc cggaggaggc
      541 actttctcag cggaacgcca cagcagctca gcacggctgc tctcctgagc ggatgccact
      601 caggctttgg acctttgggt gtagagttac gaaagaacct agcctcacaa tggtggtcct
      661 ctatggtggt gttcagggag caggtcttcg cggtggactc cctccaccac gagccgggct
      721 cttctcagcc ccgggacagt gccttcaggt tagtttctcc agaatgtata cgcgaaatct
      781 tgcaagacaa agagctgagt aaggaacagc tagtggcatt tcttgagaac ttattaaaaa
      841 cttctgggaa actacgggag actcttcttc acggtgcctt agagcactat gttcactgcc
      901 tggatctggt aaacagaaag ctaccttacg gccttgctca gattggagtg tgtttccatc
      961 ctgttttgaa ccctaaggag atacccaaca gtgttacaag agttggcgaa aagacagaag
     1021 cttcgttggt gtggtttact cccacaagga cttcgagcca gtggcttgat ttctggttgc
     1081 gccatcggct tctgtggtgg agaaagtttg ctgtgagtcc ctctaacttc agcagcgtcg
     1141 actgtcagga tggatcgggt cgtaagggat gcaggctcta ctacagcttt ccctggggga
     1201 aggagccact ggagaccctg tgggatctag gagagcaaga actcctaaac gtgtatcctg
     1261 gagatgtgtc tgcgatacag ggtcgagatg ggcggaaaaa cgtggttcct tccgttctgt
     1321 ctgtaaatgg ggacctagac ctgggcacgc tggcttacct ctacgattcc ttccagttca
     1381 cagagaactc cttttcgagg aagaagagcc tccagagaaa ggtgcttaaa cttcaccctt
     1441 gtttagcccc tattaaggtg gctctagatg tggggaaagg cccaaccgtg gaactccgac
     1501 aggtttgcca agggctactc aatgagttac tagaaaatgg gattgctgtg tggcctggtt
     1561 atttggaaac tgcacagtcc tcattagagc aactctattc aaagtacgat gagatgagta
     1621 tcctcttcac ggttctaatc actgaaacca ccttggaaag tgggctgatc cagcttcgaa
     1681 gccgagacac cacgatgaag gagatgatgc acatatccag gctgagagac ttcctggtca
     1741 agtacctggc gtcagctggg aaggcatagg cctccgcact ctaatggaca gtctctgtta
     1801 gttcattacc ttatgttaat acggttttgg ccttttgtat ttatacagta gacacttttg
     1861 gttctttctt cg
//
