LOCUS       NM_001107621            2259 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus protein O-fucosyltransferase 2 (Pofut2), mRNA.
ACCESSION   NM_001107621 XM_001079564 XM_228073
VERSION     NM_001107621.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Vasudevan,D., Takeuchi,H., Johar,S.S., Majerus,E. and
            Haltiwanger,R.S.
  TITLE     Peters plus syndrome mutations disrupt a noncanonical ER
            quality-control mechanism
  JOURNAL   Curr Biol 25 (3), 286-295 (2015)
   PUBMED   25544610
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Chen,C.I., Keusch,J.J., Klein,D., Hess,D., Hofsteenge,J. and Gut,H.
  TITLE     Structure of human POFUT2: insights into thrombospondin type 1
            repeat fold and O-fucosylation
  JOURNAL   EMBO J 31 (14), 3183-3197 (2012)
   PUBMED   22588082
REFERENCE   3  (bases 1 to 2259)
  AUTHORS   Du,J., Takeuchi,H., Leonhard-Melief,C., Shroyer,K.R., Dlugosz,M.,
            Haltiwanger,R.S. and Holdener,B.C.
  TITLE     O-fucosylation of thrombospondin type 1 repeats restricts
            epithelial to mesenchymal transition (EMT) and maintains epiblast
            pluripotency during mouse gastrulation
  JOURNAL   Dev Biol 346 (1), 25-38 (2010)
   PUBMED   20637190
REFERENCE   4  (bases 1 to 2259)
  AUTHORS   Ricketts,L.M., Dlugosz,M., Luther,K.B., Haltiwanger,R.S. and
            Majerus,E.M.
  TITLE     O-fucosylation is required for ADAMTS13 secretion
  JOURNAL   J Biol Chem 282 (23), 17014-17023 (2007)
   PUBMED   17395589
REFERENCE   5  (bases 1 to 2259)
  AUTHORS   Menzel,O., Vellai,T., Takacs-Vellai,K., Reymond,A., Mueller,F.,
            Antonarakis,S.E. and Guipponi,M.
  TITLE     The Caenorhabditis elegans ortholog of C21orf80, a potential new
            protein O-fucosyltransferase, is required for normal development
  JOURNAL   Genomics 84 (2), 320-330 (2004)
   PUBMED   15233996
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AA965207.1, CV103570.1, DY565624.1, CV117486.1 and BQ190934.1.
            
            On Jan 9, 2008 this sequence version replaced NM_001107621.1.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA5760389
                              [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-430               AA965207.1         13-442              c
            431-902             CV103570.1         163-634
            903-1230            DY565624.1         169-496
            1231-1955           CV117486.1         39-763
            1956-2259           BQ190934.1         14-317              c
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="20"
                     /map="20p12"
     gene            1..2259
                     /gene="Pofut2"
                     /note="protein O-fucosyltransferase 2"
                     /db_xref="GeneID:309686"
                     /db_xref="RGD:1307334"
     exon            1..199
                     /gene="Pofut2"
                     /inference="alignment:Splign:2.1.0"
     CDS             69..1358
                     /gene="Pofut2"
                     /EC_number="2.4.1.221"
                     /note="GDP-fucose protein O-fucosyltransferase 2"
                     /codon_start=1
                     /product="GDP-fucose protein O-fucosyltransferase 2
                     precursor"
                     /protein_id="NP_001101091.2"
                     /db_xref="GeneID:309686"
                     /db_xref="RGD:1307334"
                     /translation="MAALSVVCLLLAAASWRPVSASGEEFWPGQSAADILSGAASRRR
                     YLLYDVNPPEGFNLRRDVYIRVASLLKTLLKTEEWVLVLPPWGRLYHWQSPDIHQVRI
                     PWSEFFDLPSLNKNIPVIEYEQFIAESGGPFIDQVYVLQGYAEGWKEGTWEEKVDERP
                     CIDPLLYSQDKHEYFRGWFWGYEETRGLNVSCLSVQGSASVIAPVLLKNTSARSVMLD
                     RAENLLHDHYGGREYWDTRRSMVFAKHLRAVGDEFRSQHLNSTDAADKMVLEEDWTRM
                     KVKLGSALGGPYLGVHLRRKDFIWGHREDVPSLEGAVKKIRSLMKTHQLDKVFVATDA
                     IRKEQEELRKLLPEMVRFEPTWEELELYKDGGVAIIDQWICAHARFFIGTSVSTFSFR
                     IHEEREILGLDPKTTYNRFCGDQEKACEQPTHWKIAY"
     exon            200..450
                     /gene="Pofut2"
                     /inference="alignment:Splign:2.1.0"
     exon            451..595
                     /gene="Pofut2"
                     /inference="alignment:Splign:2.1.0"
     exon            596..706
                     /gene="Pofut2"
                     /inference="alignment:Splign:2.1.0"
     exon            707..773
                     /gene="Pofut2"
                     /inference="alignment:Splign:2.1.0"
     exon            774..899
                     /gene="Pofut2"
                     /inference="alignment:Splign:2.1.0"
     exon            900..1080
                     /gene="Pofut2"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..1204
                     /gene="Pofut2"
                     /inference="alignment:Splign:2.1.0"
     exon            1205..2259
                     /gene="Pofut2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tccgccgggc agacaggcgc gcttccggag cggggcgcgg cagccggaag tgcgtggccg
       61 ccggggccat ggcggcgctc agcgtcgtct gtctgctgct ggcagcagca tcctggcggc
      121 cggtctcggc ttcaggagaa gaattctggc ctggccagtc ggcggcggac atcctgtcgg
      181 gggcggcatc ccgcagacgg taccttctgt atgatgtcaa ccccccggaa ggctttaatc
      241 tccgcaggga tgtctatatc cgggtggcat ccttgctaaa gacgctgctg aagacagagg
      301 agtgggtgct tgtcctgccc ccatggggcc gcctctacca ctggcaaagc cctgatatcc
      361 atcaggtccg gattccctgg tctgagtttt ttgacctccc gagtctcaat aaaaacatcc
      421 ccgttataga gtacgagcag ttcattgcag agtccggcgg gccctttatt gaccaggtgt
      481 acgtcctgca aggctacgcc gagggctgga aggagggcac ctgggaggag aaggtggacg
      541 agcggccctg catagaccct ctgctgtact cacaggacaa gcatgagtat ttcagaggct
      601 ggttttgggg ctacgaagaa acacggggtc tgaatgtctc ctgcctgtcc gtccaggggt
      661 cggcttctgt catcgcgcct gtactcttaa aaaacacatc tgctcggtct gtgatgctgg
      721 accgagctga aaacctgctt catgaccact atggaggcag agagtactgg gacacccggc
      781 gcagcatggt gtttgcaaag cacttgcggg ccgtgggaga cgaattcagg agccagcacc
      841 tcaactccac ggatgccgcc gataagatgg tcctggagga agactggacc aggatgaagg
      901 tcaaactggg ctcagcgcta ggcggcccct accttggcgt ccacctgaga aggaaggact
      961 tcatctgggg ccacagggag gatgtgccca gcctagaagg tgccgtgaag aagatccgca
     1021 gcctcatgaa gactcaccag ctggacaagg tgttcgtggc cacagatgcc atcaggaagg
     1081 aacaggaaga actgaggaag ctgctgccgg aaatggtgag gtttgagccc acgtgggagg
     1141 agctggaact gtacaaggac ggaggcgtcg ccatcatcga ccagtggatc tgcgctcatg
     1201 ccaggttttt tattggtacc tccgtttcca cgttttcctt tcggattcat gaagagagag
     1261 agatcctggg gttggacccc aagacgacat acaaccggtt ctgtggagac caggagaaag
     1321 cgtgcgagca gcccacgcac tggaagattg cgtactgagg gccacctatc caccaccagt
     1381 cactcggtca cagggtcccc gaggaggctc ccacttgggt aacaacacat ctgaagcccg
     1441 tgttcccttg tgtccatggg ctgacatcta cctgtgtcta ggctctgcca ccttcaggat
     1501 tcacaaatgt gtcttctctc agtacctgag aacccatgtc cccatcacag atgtcacatg
     1561 cgtgcaagtc cagaaatggg agaacatttg tcagctccgg cacaagcctt tcaaccgcct
     1621 tcctgagcaa gttgagaatg gccagtgtgt ggcaacccgt acaggcgtgg atgcgggctc
     1681 ctgaggtgtt ggggtgactg acttctgaga tcacttcaca catggccagt gtgagcagac
     1741 accaggtttt gcctcagcct ccacctgtat ggaggttcct tctgagtgaa cgactcaggc
     1801 actgctgtca tctgagtgct gggaacctta ggacagccct tgagccttgc catagactct
     1861 ctcacctcac gtggattccc cagactcgtt gcacactgct gagaaataga atggagaggg
     1921 aacagaagtg gccttcaaaa ggccacatct gagacatgcc cctgctttat gaaacccttt
     1981 cctaggcctc acgctgcacc atgggaggca gagtcagcac agccttggcc agggcatccc
     2041 caggtccttg acctggtgct taccagtaca cccctccccc cagtgctgtg cactgagcac
     2101 ccctgccccc tcagctactg cccaggtctc catcccaagc accaaggtag tccctgagca
     2161 cagactcagc caaccaagta gaatcacgta tttattttcc atttgtacat ccaagagtat
     2221 gaataaaata ctttttcaag aaagctgcaa tcctagaaa
//
