LOCUS       NM_001002278            5124 bp    mRNA    linear   ROD 18-FEB-2024
DEFINITION  Rattus norvegicus protein O-fucosyltransferase 1 (Pofut1), mRNA.
ACCESSION   NM_001002278 XM_230746
VERSION     NM_001002278.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   McMillan,B.J., Zimmerman,B., Egan,E.D., Lofgren,M., Xu,X.,
            Hesser,A. and Blacklow,S.C.
  TITLE     Structure of human POFUT1, its requirement in ligand-independent
            oncogenic Notch signaling, and functional effects of Dowling-Degos
            mutations
  JOURNAL   Glycobiology 27 (8), 777-786 (2017)
   PUBMED   28334865
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   3  (bases 1 to 5124)
  AUTHORS   Schuster-Gossler,K., Harris,B., Johnson,K.R., Serth,J. and
            Gossler,A.
  TITLE     Notch signalling in the paraxial mesoderm is most sensitive to
            reduced Pofut1 levels during early mouse development
  JOURNAL   BMC Dev Biol 9, 6 (2009)
   PUBMED   19161597
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5124)
  AUTHORS   Fiuza,U.M. and Arias,A.M.
  TITLE     Cell and molecular biology of Notch
  JOURNAL   J Endocrinol 194 (3), 459-474 (2007)
   PUBMED   17761886
  REMARK    Review article
REFERENCE   5  (bases 1 to 5124)
  AUTHORS   Luo,Y. and Haltiwanger,R.S.
  TITLE     O-fucosylation of notch occurs in the endoplasmic reticulum
  JOURNAL   J Biol Chem 280 (12), 11289-11294 (2005)
   PUBMED   15653671
REFERENCE   6  (bases 1 to 5124)
  AUTHORS   Martinez-Duncker,I., Mollicone,R., Candelier,J.J., Breton,C. and
            Oriol,R.
  TITLE     A new superfamily of protein-O-fucosyltransferases,
            alpha2-fucosyltransferases, and alpha6-fucosyltransferases:
            phylogeny and identification of conserved peptide motifs
  JOURNAL   Glycobiology 13 (12), 1C-5C (2003)
   PUBMED   12966037
REFERENCE   7  (bases 1 to 5124)
  AUTHORS   Haines,N. and Irvine,K.D.
  TITLE     Glycosylation regulates Notch signalling
  JOURNAL   Nat Rev Mol Cell Biol 4 (10), 786-797 (2003)
   PUBMED   14570055
  REMARK    Review article
REFERENCE   8  (bases 1 to 5124)
  AUTHORS   Shi,S. and Stanley,P.
  TITLE     Protein O-fucosyltransferase 1 is an essential component of Notch
            signaling pathways
  JOURNAL   Proc Natl Acad Sci U S A 100 (9), 5234-5239 (2003)
   PUBMED   12697902
REFERENCE   9  (bases 1 to 5124)
  AUTHORS   Wang,Y., Shao,L., Shi,S., Harris,R.J., Spellman,M.W., Stanley,P.
            and Haltiwanger,R.S.
  TITLE     Modification of epidermal growth factor-like repeats with O-fucose.
            Molecular cloning and expression of a novel GDP-fucose protein
            O-fucosyltransferase
  JOURNAL   J Biol Chem 276 (43), 40338-40345 (2001)
   PUBMED   11524432
REFERENCE   10 (bases 1 to 5124)
  AUTHORS   Wang,Y., Lee,G.F., Kelley,R.F. and Spellman,M.W.
  TITLE     Identification of a GDP-L-fucose:polypeptide fucosyltransferase and
            enzymatic addition of O-linked fucose to EGF domains
  JOURNAL   Glycobiology 6 (8), 837-842 (1996)
   PUBMED   9023546
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000119.1.
            
            [WARNING] On Apr 3, 2024 this sequence was replaced by
            NM_001002278.3.
            
            On Nov 3, 2022 this sequence version replaced NM_001002278.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ781499.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-209               JACYVU010000119.1  15363768-15363976
            210-331             JACYVU010000119.1  15365841-15365962
            332-514             JACYVU010000119.1  15369654-15369836
            515-627             JACYVU010000119.1  15370848-15370960
            628-820             JACYVU010000119.1  15380117-15380309
            821-1063            JACYVU010000119.1  15381631-15381873
            1064-5124           JACYVU010000119.1  15385843-15389903
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q41"
     gene            1..5124
                     /gene="Pofut1"
                     /gene_synonym="Fut12; O-FucT-1"
                     /note="protein O-fucosyltransferase 1"
                     /db_xref="GeneID:311551"
                     /db_xref="RGD:1303001"
     exon            1..209
                     /gene="Pofut1"
                     /gene_synonym="Fut12; O-FucT-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..1252
                     /gene="Pofut1"
                     /gene_synonym="Fut12; O-FucT-1"
                     /EC_number="2.4.1.221"
                     /function="adds fucose to Ser or Thr"
                     /note="GDP-fucose protein O-fucosyltransferase 1;
                     peptide-O-fucosyltransferase 1"
                     /codon_start=1
                     /product="GDP-fucose protein O-fucosyltransferase 1
                     precursor"
                     /protein_id="NP_001002278.1"
                     /db_xref="GeneID:311551"
                     /db_xref="RGD:1303001"
                     /translation="MGAAAWAPSHLLLRASFLLLLLLLPLPLRGRAGGSWDLAGYLLY
                     CPCMGRFGNQADHFLGSLAFAKLLNRTLAVPPWIEYQHHKPPFTNLHVSYQKYFKLEP
                     LQAYHRVISLEDFMEKLAPFHWPPEKRVAYCFEVAAQRSPDKKTCPMKEGNPFGPFWD
                     QFHVSFNKSELFTGISFSASYKEQWIQRFPPKEHPVLALPGAPAQFPVLEEHRELQKY
                     MVWSDEMVRTGEAQISTHLVRPYVGIHLRIGSDWKNACAMLKDGTAGSHFMASPQCVG
                     YSRSTATPLTTTMCLPDLKEIQRAVKLWVRALDARSVYIATDSESYVSEIQQLFKEKV
                     KVVSLKPEVAQIDLYILGQADHFIGNCVSSFTAFVKRERDLHGRPSSFFGMDRPSQLR
                     DEF"
     exon            210..331
                     /gene="Pofut1"
                     /gene_synonym="Fut12; O-FucT-1"
                     /inference="alignment:Splign:2.1.0"
     exon            332..514
                     /gene="Pofut1"
                     /gene_synonym="Fut12; O-FucT-1"
                     /inference="alignment:Splign:2.1.0"
     exon            515..627
                     /gene="Pofut1"
                     /gene_synonym="Fut12; O-FucT-1"
                     /inference="alignment:Splign:2.1.0"
     exon            628..820
                     /gene="Pofut1"
                     /gene_synonym="Fut12; O-FucT-1"
                     /inference="alignment:Splign:2.1.0"
     exon            821..1063
                     /gene="Pofut1"
                     /gene_synonym="Fut12; O-FucT-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1064..5124
                     /gene="Pofut1"
                     /gene_synonym="Fut12; O-FucT-1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 actcgcgcct cttctttccc ctccccgcct cctggccgcg gcgctctggg gctcggccgt
       61 cgacatgggc gccgccgcgt gggcaccgtc acacctgctg ctgcgggcgt cttttctgct
      121 tctgctgctg ttgctgccgc tgccgctccg cgggcgggcg gggggctcct gggatctggc
      181 cggttacctg ctctactgtc cctgcatggg gcgctttggg aaccaggctg atcacttctt
      241 gggctctctg gcatttgcaa agctgctgaa ccgcaccttg gctgtacctc catggattga
      301 gtaccaacat cacaagcctc ccttcaccaa cctccatgtg tcttaccaaa agtacttcaa
      361 actggagcct ctccaagcct accatcgggt tatcagcctg gaggacttca tggaaaagct
      421 ggcacccttt cactggcccc ctgagaagcg ggtggcatac tgctttgagg tggcagccca
      481 gcgaagtcct gataagaaga catgtcccat gaaggaagga aatccttttg ggccattctg
      541 ggaccagttt catgtgagtt tcaataagtc agagttgttc acaggcattt ccttcagcgc
      601 ctcctacaaa gaacaatgga tccagagatt tcctccaaag gagcatccag tgctcgcact
      661 accgggggcc ccagcccaat tccccgtcct ggaggagcac agggaactcc agaagtacat
      721 ggtatggtca gatgagatgg tgaggacagg agaggcccag atcagtaccc acctcgtccg
      781 gccctatgtg ggcattcatc tgcgcattgg ctccgactgg aagaatgcct gtgccatgtt
      841 gaaggatggg actgcagggt cacacttcat ggcttcccct cagtgtgtgg gctacagccg
      901 cagcacagcg actcccctca ccactaccat gtgcctcccc gacctgaagg aaatccaacg
      961 ggctgtgaag ctttgggtga gagcactgga tgccaggtcg gtctacatcg ccacagactc
     1021 tgagagctat gtgtcagaga tccagcagct cttcaaagag aaggtgaagg ttgtgagcct
     1081 gaaacccgag gtggcccaga ttgacctgta catcctcggt caggctgacc acttcattgg
     1141 aaactgtgtc tcctcattca ctgccttcgt gaagcgggag cgggacctcc atgggaggcc
     1201 gtcatccttc tttggcatgg acagaccctc ccagcttcgg gatgaatttt gatcccatct
     1261 ggagcccccc ccccccccaa ccagacttgg ctgctaaggg tgctcctggg actgcaagct
     1321 cctttcctct cctgccagag atggagaaca gtaccaaggg ctccctagaa gagaaggcat
     1381 tccatcccag gccttggttc ccatgctcca tagccagagc tgccttgggc tgcatgtgct
     1441 tctagagaac atgcacacaa agcagcccac ctctagcctg gggctttccc tgctccgagt
     1501 ggcctggagt gctcaactca ctcttgagca gttactttat aaaaaggaag atttttttaa
     1561 tagggtttct tggatgggag gttgggggag gaacaaaggg gtaaggttaa gatgatctct
     1621 tatagcccag gctggccttg aactcccagt cttcctgcct ctacctccta agtgctgaga
     1681 ttactgatgt ctaccacgac actcagctga tctctgtagt tttgatgcaa agccatgact
     1741 acctgaaaat ttccctttgt ttttaaaaaa tcagtggagt ccattggcac agatacctaa
     1801 agtgacctta taaatgcaga cagagccagg gagcaggtgg gcccattggc tacttttctg
     1861 ggatccccta aagggccatc agacttcccc actgccaggc ctgggctcct ttgttgtgaa
     1921 cacaacaggc agcagttagc agccctgttt tccagtgctc cagggacagg taacacagca
     1981 ggtggctgtg ttcaggccta gcagcatcac tgtccggtga ggctggcagg gtgctctgaa
     2041 ggctgctgtg tccaagaccc tcccagcctc tgctctgtgg tcgcagccct cacagcagcc
     2101 attccccagt actgctcact aggcctctga tgcatgcaca gaggccatgt gaagccccag
     2161 cctgaagcag tctttgccct ccagcgagtg caccacctgc tcgtgtggtg aggtccttct
     2221 gatgctcctc tcattcgtaa gaagtgcact ctaagggact gatcaggtgg ctctcggttc
     2281 tgccatgcgc agggacattg agaggctgga gccccatcct aacaggcata gtatttctga
     2341 agagtaggtg ctgggactct tgtctgtgcc aagcctgcac tctttgggcc agtctctccc
     2401 ctgtaagtgc tgtcgggtgg gttataagcc ccaagtccgc tagaaaccgt tctaaacctg
     2461 cactataaca aggcccctct gcagccatct ggggcttaag tgtgcttatt ccttgtgctc
     2521 ccagatcagg cactgtgctg ggccttatgg gcatcctctt ccttaccttc ccctggtatt
     2581 atccttttct ttctgacaaa agtgaacgtt cagagaagtg ttctccctgg cagggctgca
     2641 cagctgtgga gggtaagcca gacccactgg gatctgtgct gcctgcctcc caggacacat
     2701 tgtgcacgga aggcactcgg ggagtgagga gccaccctga ctgctgttgg catctgctgc
     2761 cagcgctagt aggaagcgac ataaagagag gaagccttgg gtagccatag ttacagtttt
     2821 atagatttta ttcaggaaat gtttactgat cacttacagt gtgctaggaa ggagggttgc
     2881 aacagagaaa gcctcaaagg atcaggtttg caatcagagg aaaacaaagt gtctatgctg
     2941 tggaagggaa gtgctctgaa gaaaagccaa gggtactggc acagggaggc tattttagag
     3001 gaatcacctt aaggcgtaga gagaacgcac tctgctaagg aggccgcaca tttccagcag
     3061 caagcatggc acgtgccaag gccctgaggc aggagtgggg gcagcaaggg aacatcagta
     3121 gatgagcaga aaggtgacat cccatggatt cactttgttt gggaccaaag agagtgtggc
     3181 ctgtttatga gaaccctgtc tcgtgcttag agaagtagga gaaggggagt agagcagagg
     3241 ctggtgctgc cggtgggcaa gaggcttggc cattgtgctg gaggtgggag aagagatcac
     3301 atttgagtat atcaagttat tttttttttt tttttttttt ttggttcttt ttttcggagc
     3361 tggggatcga acccagggcc ttgcgcttcc taggtaagcg ctctaccact gagctaaatc
     3421 cccagcccca tcaagttatt tttgtaattc agtctctttt taaagatacc aatctgcata
     3481 accgtgtaac atagaccatt taatgttgtg ggttttttac ataaccacat accatatttt
     3541 ccctgtatga aagtagctga atggatgctt ttaatcatgt aaacttccac tgagggtttc
     3601 acagtttgtg acccaccttc tatgaggtca ttgcccagct gttcctgttg ggattttttc
     3661 caagacaggg tttctctgtg cagcctgtgc tgtcttagaa tattctgtag accaggctgg
     3721 cctctagttc acagaggtcg cctgcctctg tcccctgagt gctggcatta catgagcgcc
     3781 accgttgccc agttgctgtt gtgatttgaa aactactgcc tacaaagact tttttaaatt
     3841 taagatacaa attatgataa attcacagaa gtgaatttgt tcaatacaag aatgtatatg
     3901 ataagaaaat tcaactatgc tcaaaaattg tggatttaaa aaactgagct cacagtgtta
     3961 acaattcatt ttaccagcta gtttcattta ttccctactc ctagtctgtc tagagacttg
     4021 tgttgtttgt ttcattttgt actttgtggt agggtctcct ctcggccagg ctggcctgga
     4081 acttttgacg tagtccaggc ttatcatgaa atcttagtcc tcctgtctca gccttacaag
     4141 gctgtgatta cagttgtgac catcggttcc tcctgagtct ttgaatgaat tgcatgggtg
     4201 cccaggaaag aggcaacaga agctgctgta gtaaggaagg aaggagggag cctcaggagg
     4261 aggttgagct caggcaggcc tgttttctcc tccaaattct aggtgctgag gcagtggata
     4321 tcttctctgt ggtatcctgc agcacaaggc acggaaagcc aatcacagct gagcaatcca
     4381 agtggctgga gccaaaagcc cccaggaacg tgcatttttg caagctcctg taaaaggccc
     4441 aaggcctgcg gtgtggtcac cgaggggaac caccctaaat gcaaagtccc agttgagcct
     4501 tttagaccta gcctgtaatc tcagctactt tggaggttaa gacaggagac tcacattcta
     4561 agcctacctg gcaacttaag tgagactgtc tcaaaataat aaggcttgaa aaaggagaag
     4621 tatgattcag ttagcggaat gtttgctcac tacaacagcc ctcaggagta caggggaaag
     4681 aatccatcca gttacacagg ataccgtatc ttggccacac tggcctggca acaaagcagg
     4741 gccttgtgca ctagcgtttg cattctgttt tggctggttt ttgtcaactt gacacaaacg
     4801 actcatctga gaagagagag cctcagtgga gaaaatgccc ctatcagatt gtcctgtagg
     4861 caagtctgtg ggtattttct tgattggtaa ttagtaaagc tgggtggggt gtcaccttta
     4921 atcccagaac tgggcaggca gaggcaggca gaggctggac gatctctgag ttcaaggcca
     4981 gccaggtctc taaatggtct ggagcacacc agtaagcagt gttcctccac agtccgcttc
     5041 agttcctgat ctgagtacct ggcttggcct ctcttcataa tggactacac aaactgtaaa
     5101 gtgaaataaa gcctttctcc ccca
//
