LOCUS       NM_001108020            4604 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus patatin-like phospholipase domain containing 8
            (Pnpla8), mRNA.
ACCESSION   NM_001108020 XM_001078026 XM_003750150 XM_003754170 XM_234092
VERSION     NM_001108020.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Mancuso,D.J., Han,X., Jenkins,C.M., Lehman,J.J., Sambandam,N.,
            Sims,H.F., Yang,J., Yan,W., Yang,K., Green,K., Abendschein,D.R.,
            Saffitz,J.E. and Gross,R.W.
  TITLE     Dramatic accumulation of triglycerides and precipitation of cardiac
            hemodynamic dysfunction during brief caloric restriction in
            transgenic myocardium expressing human calcium-independent
            phospholipase A2gamma
  JOURNAL   J Biol Chem 282 (12), 9216-9227 (2007)
   PUBMED   17213206
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Yan,W., Jenkins,C.M., Han,X., Mancuso,D.J., Sims,H.F., Yang,K. and
            Gross,R.W.
  TITLE     The highly selective production of 2-arachidonoyl
            lysophosphatidylcholine catalyzed by purified calcium-independent
            phospholipase A2gamma: identification of a novel enzymatic mediator
            for the generation of a key branch point intermediate in eicosanoid
            signaling
  JOURNAL   J Biol Chem 280 (29), 26669-26679 (2005)
   PUBMED   15908428
REFERENCE   3  (bases 1 to 4604)
  AUTHORS   Murakami,M., Masuda,S., Ueda-Semmyo,K., Yoda,E., Kuwata,H.,
            Takanezawa,Y., Aoki,J., Arai,H., Sumimoto,H., Ishikawa,Y.,
            Ishii,T., Nakatani,Y. and Kudo,I.
  TITLE     Group VIB Ca2+-independent phospholipase A2gamma promotes cellular
            membrane hydrolysis and prostaglandin production in a manner
            distinct from other intracellular phospholipases A2
  JOURNAL   J Biol Chem 280 (14), 14028-14041 (2005)
   PUBMED   15695510
REFERENCE   4  (bases 1 to 4604)
  AUTHORS   Tanaka,H., Takeya,R. and Sumimoto,H.
  TITLE     A novel intracellular membrane-bound calcium-independent
            phospholipase A(2)
  JOURNAL   Biochem Biophys Res Commun 272 (2), 320-326 (2000)
   PUBMED   10833412
REFERENCE   5  (bases 1 to 4604)
  AUTHORS   Mancuso,D.J., Jenkins,C.M. and Gross,R.W.
  TITLE     The genomic organization, complete mRNA sequence, cloning, and
            expression of a novel human intracellular membrane-associated
            calcium-independent phospholipase A(2)
  JOURNAL   J Biol Chem 275 (14), 9937-9945 (2000)
   PUBMED   10744668
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000006.1 and FQ211866.1.
            
            On or before Aug 21, 2019 this sequence version replaced
            XM_003750150.4, XM_003754170.4, NM_001108020.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on orthologous alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360190.1953945.1,
                                           SRR26360172.222117.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-145               JAXUCZ010000006.1  67056790-67056934
            146-1271            JAXUCZ010000006.1  67083952-67085077
            1272-1421           JAXUCZ010000006.1  67085165-67085314
            1422-1573           JAXUCZ010000006.1  67088750-67088901
            1574-1668           JAXUCZ010000006.1  67094839-67094933
            1669-1840           JAXUCZ010000006.1  67097074-67097245
            1841-1898           JAXUCZ010000006.1  67100127-67100184
            1899-2093           JAXUCZ010000006.1  67106248-67106442
            2094-2289           JAXUCZ010000006.1  67113352-67113547
            2290-4580           JAXUCZ010000006.1  67116423-67118713
            4581-4604           FQ211866.1         2777-2800
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q21"
     gene            1..4604
                     /gene="Pnpla8"
                     /gene_synonym="RGD1311444"
                     /note="patatin-like phospholipase domain containing 8"
                     /db_xref="GeneID:314075"
                     /db_xref="RGD:1311444"
     exon            1..145
                     /gene="Pnpla8"
                     /gene_synonym="RGD1311444"
                     /inference="alignment:Splign:2.1.0"
     exon            146..1271
                     /gene="Pnpla8"
                     /gene_synonym="RGD1311444"
                     /inference="alignment:Splign:2.1.0"
     CDS             234..2564
                     /gene="Pnpla8"
                     /gene_synonym="RGD1311444"
                     /EC_number="3.1.1.5"
                     /note="iPLA2-gamma; patatin-like phospholipase
                     domain-containing protein 8; intracellular
                     membrane-associated calcium-independent phospholipase A2
                     gamma"
                     /codon_start=1
                     /product="calcium-independent phospholipase A2-gamma"
                     /protein_id="NP_001101490.2"
                     /db_xref="GeneID:314075"
                     /db_xref="RGD:1311444"
                     /translation="MSINLTLDIYIYFLNNARSFCGKQRSKQLNFLCSKQYWRMNHVN
                     VHREFHTSKKSCKWNRSEAHCSKHWHSSSNHGVHIGIVKLSTSAPKGLTKVSIHMSRI
                     KSTLNSVSKAIFGSQNEMVSRLAQFKPSSRIFRKVSDRGWLKHKNVKQAIESLKNYSD
                     KSAEKNSFAEQKSYFADKEEGSDKHSLYHYAYRITTRFGESFYFLANHINSYFKNKEK
                     MSQIKEDRQLQDKPCLEESKSISPSPDILTDRPDSGPPLNVEDKLSSSTQLPEALPVS
                     TKQSIANFLSRPTEGVQALVGGYIGGLVPKLKSDPKSQPEEEEEPSKTDEPICKDKKA
                     EEKKRVLLQREKIIARVSIDNRTRALVQALRRTTDPKLCITRVEELTFHLLEFPEGKG
                     VAVKEKIIPYLLRLRQIKDETLQAAVREILALIGYVDPVKGRGIRILAIDGGGTRGVV
                     ALQTLRKLVELTQKPIHQLFDYICGVSTGAILAFMLGLFHMPLDECEELYRKLGSDVF
                     TQNVIVGTVKMSWSHAFYDSHTWEKILKDKVGSALMIETARDPLCPKVAAVSTIVNRG
                     QTPKAFVFRNYGHFPGTNSHYLGGCQYKMWQAIRASSAAPGYFAEYALGNDLHQDGGL
                     LLNNPSALALHECKCIWPDTPLECIVSLGTGRYESDVRNTTTYTSLKTKLSNVISSAT
                     DTEEVHIMLDGLLPADTYFRFNPVICENIPLDESRNEKLDQLQLEGMKYLERNDEKMK
                     KLAKILSREKTTLQKISDWIKLKSDMYEGLPFFSKL"
     exon            1272..1421
                     /gene="Pnpla8"
                     /gene_synonym="RGD1311444"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..1573
                     /gene="Pnpla8"
                     /gene_synonym="RGD1311444"
                     /inference="alignment:Splign:2.1.0"
     exon            1574..1668
                     /gene="Pnpla8"
                     /gene_synonym="RGD1311444"
                     /inference="alignment:Splign:2.1.0"
     exon            1669..1840
                     /gene="Pnpla8"
                     /gene_synonym="RGD1311444"
                     /inference="alignment:Splign:2.1.0"
     exon            1841..1898
                     /gene="Pnpla8"
                     /gene_synonym="RGD1311444"
                     /inference="alignment:Splign:2.1.0"
     exon            1899..2093
                     /gene="Pnpla8"
                     /gene_synonym="RGD1311444"
                     /inference="alignment:Splign:2.1.0"
     exon            2094..2289
                     /gene="Pnpla8"
                     /gene_synonym="RGD1311444"
                     /inference="alignment:Splign:2.1.0"
     exon            2290..4581
                     /gene="Pnpla8"
                     /gene_synonym="RGD1311444"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggcgtagcgg agggtgaggc tgtagcgcca gtgtttgggg tccgggcgcc gcagctgcta
       61 gaaggccggg caggctgatg cggacatcat cgcaggcgga aggcaagggg cggccgcggc
      121 gcccgtgact gttgcctacg tccaggtacg accatgaatt acggaatttc ttaaatgaag
      181 cattcagaag tggaatgaga gaatgtcaca tagaaaataa agatttttaa gttatgtcta
      241 ttaatttaac tctagatata tatatttact tccttaataa tgcaagaagt ttttgtggga
      301 aacagagaag caaacagttg aatttcttgt gttctaaaca gtattggcgc atgaaccatg
      361 tcaatgtaca cagagagttt cacacaagta agaaaagctg taaatggaac agaagtgaag
      421 cacattgcag caaacactgg cactcttcaa gcaaccatgg tgtacatatt ggaattgtga
      481 aacttagcac ttctgctccc aagggactta caaaagtgag cattcatatg tcccgtatta
      541 aaagtacttt gaattctgtt tcaaaggcta tttttggcag tcaaaatgaa atggtttcac
      601 ggttagctca gtttaagcca agttctcgaa tattcagaaa agtatcagat aggggatggt
      661 taaaacataa aaatgttaag caagccattg aatctctgaa aaactatagt gataaatcag
      721 cagaaaagaa ttcttttgca gaacagaaaa gttactttgc agataaagaa gaaggttcag
      781 ataaacacag cctttaccat tacgcataca gaattaccac cagatttgga gagtcattct
      841 actttttagc aaatcatatt aattcatact tcaaaaacaa ggaaaaaatg tctcaaataa
      901 aggaagatag acagcttcag gacaaaccat gccttgaaga aagtaaatcc atttctccta
      961 gccctgacat cttgacagac aggccagact cgggacctcc cctaaatgtt gaagacaaac
     1021 tttccagttc tacccagctg cctgaggctc tcccagtatc tacaaaacaa agtattgcta
     1081 acttcctttc tcgtcccact gaaggcgtac aagccttagt tggtggttac attggtggac
     1141 ttgtacccaa attaaagtct gatcccaaga gtcaacctga agaagaagaa gagccttcta
     1201 aaactgatga gcctatctgc aaagacaaaa aagcagagga gaaaaagcgt gtgttacttc
     1261 agcgagaaaa gattattgca agagtgagta ttgataacag aactcgagcc ttagttcagg
     1321 ccttaagaag aacaaccgac ccaaagctct gtattactag ggttgaagaa ctgacttttc
     1381 atcttctaga atttcctgaa ggaaaaggag tggctgtcaa ggaaaaaatt atcccatatt
     1441 tattacggct gagacaaatt aaagatgaaa ctcttcaggc tgcagttaga gaaattttgg
     1501 ccttaattgg ttatgtagat ccagtgaaag gcagaggaat ccgaatcctc gcgattgatg
     1561 gtggaggaac aagaggtgtg gttgctcttc agactctgcg aaagttggtt gaacttactc
     1621 agaagccgat tcatcagctc tttgattaca tctgcggtgt aagcacaggg gccatattag
     1681 cattcatgct gggattgttt cacatgccgc tggatgaatg tgaggaactc tatcgaaagt
     1741 tgggctcaga tgtgttcact cagaatgtca ttgtcgggac cgtcaaaatg agttggagcc
     1801 atgcatttta tgatagtcac acgtgggaaa agatccttaa ggataaagtt ggatctgcac
     1861 taatgattga aacagcaaga gaccccttat gtcctaaggt agctgctgta agtaccatag
     1921 taaacagagg acaaacacca aaagcttttg tgttcagaaa ctatggacat tttcctggca
     1981 ccaactctca ctatctagga ggctgtcagt ataaaatgtg gcaggccatc agagcctcat
     2041 cagctgctcc aggctacttt gcagaatatg cgctggggaa tgatcttcat caggatggag
     2101 gtttgcttct gaataaccct tcagccttgg ccctgcacga atgtaaatgt atctggccag
     2161 ataccccact agagtgtata gtatccctgg gcacaggacg atatgagagt gatgtgagga
     2221 ataccacaac atacacgagc ctgaagacca agctctccaa tgtcatcagc agtgctacag
     2281 atacagagga agtccacata atgcttgatg gtctattacc tgctgacacg tattttaggt
     2341 ttaatcctgt gatctgtgaa aacatacccc tagatgaaag ccgcaatgag aagctggacc
     2401 agcttcagct ggaagggatg aagtatctag aaagaaatga tgaaaagatg aaaaaacttg
     2461 caaaaatatt aagtcgagaa aaaacaactc ttcagaagat tagtgattgg ataaaattaa
     2521 aaagtgatat gtatgaaggc cttccatttt tttcaaaatt gtgagtaggg tatacatgtg
     2581 ttcctcaagg ccatcaaaag atccataagt ttatagagga gcagtgtgat tcacatgaat
     2641 tttgtgtagg acacttagtg aaatccggga atccttgaat cagataatgc tttgagtagc
     2701 tcgaacctca cagaagatat gattggtaac aaaagttata tgggaattag gtatttaaga
     2761 tgttcataac ctttagacgt tttattacta tgctatttgg ttttagtatc tattagtctt
     2821 aaattgtttg ctgtttcaaa atgtactaat atatgtgcca aacaagcaat ggggaatgtt
     2881 acactttatg ctttcttttt tgtcatttca atcatgtgga acttgtgtga ctgttgattt
     2941 tctttcatgt caaaaggtga tttctcttta catttacacc gttagcattt ccctacgtgc
     3001 acccacaatc catgtgttac ctttcactgt aataatcatg gaaagagaga taggaaatgg
     3061 gattttctct gtttaaagta catttatgtt tgaaatatga aaaaactgga cacaattttc
     3121 tattcaaaat gattgatgga tttccagtga actttttctt gaaatttcaa gataaggttg
     3181 ttcacttttg tagatcaaat ggtatcatat catttactgt tatagaatta attaattgat
     3241 tttactgaga aaatattctt atcattagca aaaataaatt attgaagtga ccacttgttc
     3301 aatgctaatg ccattttaaa ggtttttttt ttaattgcta ataatgattg cctggtttct
     3361 tgtaacattt atgaggaaat gttagcctag ttagatttac agaagttaag catggcacca
     3421 taggccaaat atattaatct tcactgtaca attgcccttg taaatcttta catttaattg
     3481 aaatttttta tgaaaaatga aaccagggtg tgtatctttt gcttaataca caaaaactaa
     3541 cagtgctgcc tgtgttgtca tagtgtttgc agtgctgggt caccagagca atgagatggg
     3601 tgagagccct ggagtggttg agaatgtggg tattagtggg ctgaatagtc agcctctggg
     3661 catgatcttg aagacagcgg acagcttata aggttcagct ctttcatttg aaaaaggagg
     3721 aaaggtaatt gtcaaatttt gtgacttctc atttattttt taagaacaag ataaataagc
     3781 ttccagttat ttcagaagat aatttaaaca ctccttttta gcctttaaat acaaataaac
     3841 ttttatgcaa ctagactgac ttactagtat aagaaaaaat agaaaggtca gtctttatga
     3901 tctcagagct ttttgcaaaa gcaaattgtc tcggtgttca ggaggcagag gaaacatctc
     3961 tccagttcta ggctattctg gtgtatatag cgagttccag tacagtcaga gaactacata
     4021 gtagaggaac tgtatttcaa acaatcaaaa aaccaaacag tcaacaatta cacttgtagc
     4081 atatactact tctatgtttg tgaaagacaa aagtggttga caataatcca gaagtccatc
     4141 ctgttatctg agaagaaagg acaggcatag tatatcatcc cattgaatgc ttattaggtg
     4201 tggtcctagc tacgtgcgga agaaaggagg gaggggaaaa aaggccatat cttgacaaac
     4261 taaaatattt agaaaaatga aaaagtgatc aaatattcta cgtcaagatc tatagctttg
     4321 actttgatct ataatacctt gctaagcagg ggctttatct ttttctgttg ttctattgaa
     4381 ttattcctga gaaaagtatt ttagggtaac agtcatcttg tgtttgtgtt gatcatttga
     4441 ccactctgaa gaattcttaa tagtcactca agttgaaaaa gtaaaaacta atgaagttta
     4501 cacctccctc tgttttttag atcaggctta acatgcttga ttcttctata atgttaataa
     4561 aattgtgtca tttagaacta aaaaaaaaaa aaaaaaaaaa aaaa
//
