LOCUS       NM_001399678            1358 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus purine nucleoside phosphorylase (Pnp), mRNA.
ACCESSION   NM_001399678 XM_006251857
VERSION     NM_001399678.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Birder,L.A., Wolf-Johnston,A., Wein,A.J., Cheng,F.,
            Grove-Sullivan,M., Kanai,A.J., Watson,A.M., Stoltz,D.,
            Watkins,S.C., Robertson,A.M., Newman,D., Dmochowski,R.R. and
            Jackson,E.K.
  TITLE     Purine nucleoside phosphorylase inhibition ameliorates
            age-associated lower urinary tract dysfunctions
  JOURNAL   JCI Insight 5 (20), 140109 (2020)
   PUBMED   32910805
  REMARK    GeneRIF: Purine nucleoside phosphorylase inhibition ameliorates
            age-associated lower urinary tract dysfunctions.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1358)
  AUTHORS   Wei,X., Das,J., Fragoza,R., Liang,J., Bastos de Oliveira,F.M.,
            Lee,H.R., Wang,X., Mort,M., Stenson,P.D., Cooper,D.N., Lipkin,S.M.,
            Smolka,M.B. and Yu,H.
  TITLE     A massively parallel pipeline to clone DNA variants and examine
            molecular phenotypes of human disease mutations
  JOURNAL   PLoS Genet 10 (12), e1004819 (2014)
   PUBMED   25502805
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1358)
  AUTHORS   Buschow,S.I., van Balkom,B.W., Aalberts,M., Heck,A.J., Wauben,M.
            and Stoorvogel,W.
  TITLE     MHC class II-associated proteins in B-cell exosomes and potential
            functional implications for exosome biogenesis
  JOURNAL   Immunol Cell Biol 88 (8), 851-856 (2010)
   PUBMED   20458337
REFERENCE   5  (bases 1 to 1358)
  AUTHORS   Ho,M.C., Shi,W., Rinaldo-Matthis,A., Tyler,P.C., Evans,G.B.,
            Clinch,K., Almo,S.C. and Schramm,V.L.
  TITLE     Four generations of transition-state analogues for human purine
            nucleoside phosphorylase
  JOURNAL   Proc Natl Acad Sci U S A 107 (11), 4805-4812 (2010)
   PUBMED   20212140
REFERENCE   6  (bases 1 to 1358)
  AUTHORS   Wu,G. and Marliss,E.B.
  TITLE     Deficiency of purine nucleoside phosphorylase activity in
            thymocytes from the immunodeficient diabetic BB rat
  JOURNAL   Clin Exp Immunol 86 (2), 260-265 (1991)
   PUBMED   1834379
REFERENCE   7  (bases 1 to 1358)
  AUTHORS   Castellano,B., Gonzalez,B., Finsen,B.R. and Zimmer,J.
  TITLE     Histochemical demonstration of purine nucleoside phosphorylase
            (PNPase) in microglial and astroglial cells of adult rat brain
  JOURNAL   J Histochem Cytochem 38 (11), 1535-1539 (1990)
   PUBMED   2120328
REFERENCE   8  (bases 1 to 1358)
  AUTHORS   Ealick,S.E., Rule,S.A., Carter,D.C., Greenhough,T.J., Babu,Y.S.,
            Cook,W.J., Habash,J., Helliwell,J.R., Stoeckler,J.D., Parks,R.E.
            Jr. et al.
  TITLE     Three-dimensional structure of human erythrocytic purine nucleoside
            phosphorylase at 3.2 A resolution
  JOURNAL   J Biol Chem 265 (3), 1812-1820 (1990)
   PUBMED   2104852
REFERENCE   9  (bases 1 to 1358)
  AUTHORS   Williams,S.R., Gekeler,V., McIvor,R.S. and Martin,D.W. Jr.
  TITLE     A human purine nucleoside phosphorylase deficiency caused by a
            single base change
  JOURNAL   J Biol Chem 262 (5), 2332-2338 (1987)
   PUBMED   3029074
REFERENCE   10 (bases 1 to 1358)
  AUTHORS   Wiginton,D.A., Coleman,M.S. and Hutton,J.J.
  TITLE     Characterization of purine nucleoside phosphorylase from human
            granulocytes and its metabolism of deoxyribonucleosides
  JOURNAL   J Biol Chem 255 (14), 6663-6669 (1980)
   PUBMED   6771276
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000015.1.
            
            On Jan 12, 2022 this sequence version replaced XM_006251857.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643299.56489.1,
                                           SRR26643298.56789.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-88                JAXUCZ010000015.1  26644147-26644234
            89-258              JAXUCZ010000015.1  26647731-26647900
            259-362             JAXUCZ010000015.1  26650048-26650151
            363-538             JAXUCZ010000015.1  26650323-26650498
            539-729             JAXUCZ010000015.1  26650608-26650798
            730-1358            JAXUCZ010000015.1  26651180-26651808
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p14"
     gene            1..1358
                     /gene="Pnp"
                     /gene_synonym="Np"
                     /note="purine nucleoside phosphorylase"
                     /db_xref="GeneID:290029"
                     /db_xref="RGD:1597189"
     exon            1..88
                     /gene="Pnp"
                     /gene_synonym="Np"
                     /inference="alignment:Splign:2.1.0"
     CDS             78..947
                     /gene="Pnp"
                     /gene_synonym="Np"
                     /EC_number="2.4.2.1"
                     /note="nucleoside phosphorylase; inosine-guanosine
                     phosphorylase; inosine phosphorylase"
                     /codon_start=1
                     /product="purine nucleoside phosphorylase"
                     /protein_id="NP_001386607.1"
                     /db_xref="GeneID:290029"
                     /db_xref="RGD:1597189"
                     /translation="MENEFTYEDYQRTAEWLRSHTKHRPQVAVICGSGLGGLTAKLTQ
                     PQAFDYNEIPNFPQSTVQGHAGRLVFGFLNGRSCVMMQGRFHMYEGYSLSKVTFPVRV
                     FHLLGVDTLVVTNAAGGLNPKFEVGDIMLIRDHINLPGFCGQNPLRGPNDERFGVRFP
                     AMSDAYDRDMRQKAFNAWKQMGEQRELQEGTYIMSAGPTFETVAESCLLRMLGADAVG
                     MSTVPEVIVARHCGLRVFGFSLITNKVVMDYNNLEKASHQEVLEAGKAAAQKLEQFVS
                     ILMESIPPRERAN"
     exon            89..258
                     /gene="Pnp"
                     /gene_synonym="Np"
                     /inference="alignment:Splign:2.1.0"
     exon            259..362
                     /gene="Pnp"
                     /gene_synonym="Np"
                     /inference="alignment:Splign:2.1.0"
     exon            363..538
                     /gene="Pnp"
                     /gene_synonym="Np"
                     /inference="alignment:Splign:2.1.0"
     exon            539..729
                     /gene="Pnp"
                     /gene_synonym="Np"
                     /inference="alignment:Splign:2.1.0"
     exon            730..1358
                     /gene="Pnp"
                     /gene_synonym="Np"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aacagtcttg accggactct ggctgtgtcg ccgtggagcg gaaagggcag gatttcggcg
       61 cagccgtcgg tgacaccatg gagaacgagt tcacatacga agattatcag cgcaccgccg
      121 agtggcttcg gtctcacacg aaacatcgac ctcaagtggc agtgatctgt ggttccggct
      181 taggagggct gaccgctaaa ttaactcagc ctcaggcctt tgactacaat gagataccca
      241 actttcccca aagcacagtg caaggccatg cgggtcgcct ggtgtttgga ttcctgaatg
      301 gcagatcctg tgtgatgatg cagggccggt tccacatgta tgaagggtac tcgctgtcaa
      361 aggtgacgtt cccagtgaga gttttccatc ttctgggtgt ggacactttg gtggtcacca
      421 atgctgctgg aggactcaac cccaaatttg aagttggaga tatcatgctg atccgtgacc
      481 acatcaacct gcctgggttc tgtggccaga accctctccg aggccccaac gatgaaaggt
      541 ttggagttcg ttttcctgcc atgtctgatg cttatgaccg ggatatgagg cagaaggctt
      601 tcaatgcctg gaaacaaatg ggggagcaac gagaactaca agaaggcacc tacataatgt
      661 cagcaggtcc cacctttgag actgtggcgg agagctgtct gctaaggatg ctgggagcag
      721 atgctgttgg catgagcaca gtcccagaag ttatcgttgc gagacactgt gggctccgtg
      781 tctttggttt ctcactcatt actaacaagg ttgtcatgga ttacaacaac ttggagaagg
      841 ccagtcacca ggaagttctg gaggccggga aagcagctgc acagaaattg gagcagtttg
      901 tctccattct tatggagagc attccacccc gtgagcgtgc caactgactg gccgcggagt
      961 gatctggtgc tcccccaggg gggtccaagt agctgtctac tttggtcact tgctgggtcg
     1021 ctagtccctg ttcttagagt ctagcagaaa ggaaagagga ggtgcctacc cttcaccttc
     1081 ccgatagata tccttctact gctccgatgc ctggtcctct tacttagttg ccttctcaaa
     1141 ttaactttca ttcctctgga agagctggag cccatttccc agcacacttg ggatctatgt
     1201 atgccttcaa tcagtatctt tctacatccc gaggatgaca gacacgttct gggctcctct
     1261 gctttcccta aagccacaaa gaccaaagac caaccccata cctcttggat tttatgtagt
     1321 attataatgt tttgagaata aagagatcgg atactttt
//
