LOCUS       NM_001004259            1690 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus polynucleotide kinase 3'-phosphatase (Pnkp),
            mRNA.
ACCESSION   NM_001004259 XM_218622
VERSION     NM_001004259.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Hoch,N.C., Hanzlikova,H., Rulten,S.L., Tetreault,M., Komulainen,E.,
            Ju,L., Hornyak,P., Zeng,Z., Gittens,W., Rey,S.A., Staras,K.,
            Mancini,G.M., McKinnon,P.J., Wang,Z.Q., Wagner,J.D., Yoon,G. and
            Caldecott,K.W.
  CONSRTM   Care4Rare Canada Consortium
  TITLE     XRCC1 mutation is associated with PARP1 hyperactivation and
            cerebellar ataxia
  JOURNAL   Nature 541 (7635), 87-91 (2017)
   PUBMED   28002403
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Tahbaz,N., Subedi,S. and Weinfeld,M.
  TITLE     Role of polynucleotide kinase/phosphatase in mitochondrial DNA
            repair
  JOURNAL   Nucleic Acids Res 40 (8), 3484-3495 (2012)
   PUBMED   22210862
REFERENCE   3  (bases 1 to 1690)
  AUTHORS   Cerone,M.A., Burgess,D.J., Naceur-Lombardelli,C., Lord,C.J. and
            Ashworth,A.
  TITLE     High-throughput RNAi screening reveals novel regulators of
            telomerase
  JOURNAL   Cancer Res 71 (9), 3328-3340 (2011)
   PUBMED   21531765
REFERENCE   4  (bases 1 to 1690)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   5  (bases 1 to 1690)
  AUTHORS   Lieber,M.R.
  TITLE     The mechanism of double-strand DNA break repair by the
            nonhomologous DNA end-joining pathway
  JOURNAL   Annu Rev Biochem 79, 181-211 (2010)
   PUBMED   20192759
  REMARK    Review article
REFERENCE   6  (bases 1 to 1690)
  AUTHORS   Chappell,C., Hanakahi,L.A., Karimi-Busheri,F., Weinfeld,M. and
            West,S.C.
  TITLE     Involvement of human polynucleotide kinase in double-strand break
            repair by non-homologous end joining
  JOURNAL   EMBO J 21 (11), 2827-2832 (2002)
   PUBMED   12032095
REFERENCE   7  (bases 1 to 1690)
  AUTHORS   Karimi-Busheri,F., Daly,G., Robins,P., Canas,B., Pappin,D.J.,
            Sgouros,J., Miller,G.G., Fakhrai,H., Davis,E.M., Le Beau,M.M. and
            Weinfeld,M.
  TITLE     Molecular characterization of a human DNA kinase
  JOURNAL   J Biol Chem 274 (34), 24187-24194 (1999)
   PUBMED   10446193
REFERENCE   8  (bases 1 to 1690)
  AUTHORS   Jilani,A., Ramotar,D., Slack,C., Ong,C., Yang,X.M., Scherer,S.W.
            and Lasko,D.D.
  TITLE     Molecular cloning of the human gene, PNKP, encoding a
            polynucleotide kinase 3'-phosphatase and evidence for its role in
            repair of DNA strand breaks caused by oxidative damage
  JOURNAL   J Biol Chem 274 (34), 24176-24186 (1999)
   PUBMED   10446192
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC079307.1.
            
            On Sep 10, 2004 this sequence version replaced XM_218622.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC079307.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q22"
     gene            1..1690
                     /gene="Pnkp"
                     /note="polynucleotide kinase 3'-phosphatase"
                     /db_xref="GeneID:308576"
                     /db_xref="RGD:1303331"
     exon            1..216
                     /gene="Pnkp"
                     /inference="alignment:Splign:2.1.0"
     CDS             66..1634
                     /gene="Pnkp"
                     /codon_start=1
                     /product="bifunctional polynucleotide phosphatase/kinase"
                     /protein_id="NP_001004259.1"
                     /db_xref="GeneID:308576"
                     /db_xref="RGD:1303331"
                     /translation="MSELGSRGRLWLQSPTGGPPPIFLPSDGQALVLGRGPLTQVMDR
                     KCSRNQVELIADPETRTVAVKQLGVNPSTVGVQELKPGVSGSLSVGDVLYLVNGLYPL
                     TLRWEEISTPGSPPDTPPGNPVDPEEGKDTEPQKKRMRKSSPGWESFKQLLVFTASGV
                     KPRGKVAAFDLDGTLITTRSGKVFPTSPSDWRILYPEIPKKLQELAAEGYKLVILTNQ
                     MGIGRGKLPAEVFKAKVEAVLEKLGVPFQVLVATHAGLNRKPVSGMWDHLQEKGNEGI
                     PISIGDSVFVGDAAGRPANWAPGKKKKDFSCADRLFALNVGLPFATPEEFFLKWPAAR
                     FELPAFDPRTISSAGPLYLPESSFLLSPNPEVVVAVGFPGAGKSTFIQKHLVSAGYVH
                     VNRDTLGSWQRCVNSCQAALRQGKQVVIDNTNPDIQSRARYIQCAKDAGVPCRCFSFC
                     ATIEQARHNNRFREMTDPSHAPVSDMVMFSYRKQFEPPTLAEGFLEILQVPFRLQEHL
                     DPALQRLYCQFSEG"
     exon            217..263
                     /gene="Pnkp"
                     /inference="alignment:Splign:2.1.0"
     exon            264..560
                     /gene="Pnkp"
                     /inference="alignment:Splign:2.1.0"
     exon            561..640
                     /gene="Pnkp"
                     /inference="alignment:Splign:2.1.0"
     exon            641..698
                     /gene="Pnkp"
                     /inference="alignment:Splign:2.1.0"
     exon            699..806
                     /gene="Pnkp"
                     /inference="alignment:Splign:2.1.0"
     exon            807..878
                     /gene="Pnkp"
                     /inference="alignment:Splign:2.1.0"
     exon            879..927
                     /gene="Pnkp"
                     /inference="alignment:Splign:2.1.0"
     exon            928..998
                     /gene="Pnkp"
                     /inference="alignment:Splign:2.1.0"
     exon            999..1091
                     /gene="Pnkp"
                     /inference="alignment:Splign:2.1.0"
     exon            1092..1188
                     /gene="Pnkp"
                     /inference="alignment:Splign:2.1.0"
     exon            1189..1250
                     /gene="Pnkp"
                     /inference="alignment:Splign:2.1.0"
     exon            1251..1360
                     /gene="Pnkp"
                     /inference="alignment:Splign:2.1.0"
     exon            1361..1448
                     /gene="Pnkp"
                     /inference="alignment:Splign:2.1.0"
     exon            1449..1510
                     /gene="Pnkp"
                     /inference="alignment:Splign:2.1.0"
     exon            1511..1666
                     /gene="Pnkp"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgggtcggtt gctggctgcg gagcgagctc cacagtgagt ccgttcccct tcacagcatc
       61 caaggatgtc agagcttgga tccaggggcc gcctgtggct ccagagcccc accggtgggc
      121 caccccctat attcctgccg tcggatggcc aagccctggt tctcggtcgg ggacccctta
      181 cccaagtgat ggaccggaag tgctcccgaa accaagtgga gctgattgca gaccctgaaa
      241 cccggacagt ggcagtgaaa cagctgggtg ttaacccatc aactgttggg gtccaggagt
      301 tgaagccagg ggtgtctggc tctctaagcg tgggggatgt cctttatctg gttaatggcc
      361 tatatcccct gaccctgcgc tgggaagaga tcagcacacc aggatccccg ccagacactc
      421 caccaggcaa tcctgtggat ccagaggagg gaaaggatac tgagcctcag aaaaagcgaa
      481 tgcggaaatc ctcccctggc tgggagagct ttaagcagtt actggtgttc acagcgtctg
      541 gagtgaagcc ccggggcaag gtggctgcct ttgacctaga tgggaccctc atcaccaccc
      601 gctctggaaa ggtcttcccc actagcccta gtgactggag gattctgtac ccagagattc
      661 caaagaagct ccaggagctg gctgctgagg gctacaagct ggtaattctc accaaccaaa
      721 tgggcatcgg gagaggcaag ctgccagcag aggtgttcaa ggccaaggtg gaagctgtat
      781 tggagaaact aggggtccca tttcaggtgc tggtggcaac gcatgcaggt ctaaaccgaa
      841 agccagtgag tggcatgtgg gaccatctgc aggagaaggg caacgagggc atccccatct
      901 ccatcgggga cagtgtcttc gtgggagatg cagcagggcg cccagccaac tgggccccag
      961 ggaagaagaa aaaggatttc tcctgtgcag accgcctgtt cgccctcaat gttggcctac
     1021 cctttgccac tcctgaagag ttcttcctca aatggccagc agcccgcttt gagcttccag
     1081 cttttgaccc gaggaccatc tccagtgctg gacccctcta cctcccagag tccagtttcc
     1141 tcctgagccc caacccagag gtggtggtcg cagtaggatt ccctggggcc ggcaagtcca
     1201 ctttcatcca gaagcacctg gtatcggctg gatatgtcca tgtgaacaga gacacgctgg
     1261 gctcatggca gcgctgtgtg aactcatgcc aggccgcact gaggcagggg aagcaagtcg
     1321 tgattgataa caccaaccca gacatccaaa gtcgggctag gtacatccag tgtgccaaag
     1381 atgctggtgt gccctgccgc tgcttcagct tctgtgccac aatagagcag gcacgccaca
     1441 acaacaggtt ccgtgagatg accgaccctt cacatgcgcc ggtatcagac atggtcatgt
     1501 ttagctacag gaagcagttt gagccgccca cactggctga aggcttcctg gagatccttc
     1561 aggtcccatt ccggttacag gagcatctgg accctgcgct gcagcgacta tactgccagt
     1621 tttccgaggg ctgagtcctc aataaacact gctgctactc tgctcaaaaa aaaaaaaaaa
     1681 aaaaaaaaaa
//
