LOCUS       NM_022395               1569 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Rattus norvegicus peptidase, mitochondrial processing subunit beta
            (Pmpcb), mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_022395
VERSION     NM_022395.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Mossmann,D., Meisinger,C. and Vogtle,F.N.
  TITLE     Processing of mitochondrial presequences
  JOURNAL   Biochim Biophys Acta 1819 (9-10), 1098-1106 (2012)
   PUBMED   22172993
  REMARK    Review article
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Greene,A.W., Grenier,K., Aguileta,M.A., Muise,S., Farazifard,R.,
            Haque,M.E., McBride,H.M., Park,D.S. and Fon,E.A.
  TITLE     Mitochondrial processing peptidase regulates PINK1 processing,
            import and Parkin recruitment
  JOURNAL   EMBO Rep 13 (4), 378-385 (2012)
   PUBMED   22354088
REFERENCE   3  (bases 1 to 1569)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   4  (bases 1 to 1569)
  AUTHORS   Da Cruz,S., Xenarios,I., Langridge,J., Vilbois,F., Parone,P.A. and
            Martinou,J.C.
  TITLE     Proteomic analysis of the mouse liver mitochondrial inner membrane
  JOURNAL   J Biol Chem 278 (42), 41566-41571 (2003)
   PUBMED   12865426
REFERENCE   5  (bases 1 to 1569)
  AUTHORS   Kitada,S., Yamasaki,E., Kojima,K. and Ito,A.
  TITLE     Determination of the cleavage site of the presequence by
            mitochondrial processing peptidase on the substrate binding
            scaffold and the multiple subsites inside a molecular cavity
  JOURNAL   J Biol Chem 278 (3), 1879-1885 (2003)
   PUBMED   12433926
REFERENCE   6  (bases 1 to 1569)
  AUTHORS   Kitada,S., Kojima,K. and Ito,A.
  TITLE     Glu(191) and Asp(195) in rat mitochondrial processing peptidase
            beta subunit are involved in effective cleavage of precursor
            protein through interaction with the proximal arginine
  JOURNAL   Biochem Biophys Res Commun 287 (3), 594-599 (2001)
   PUBMED   11563836
REFERENCE   7  (bases 1 to 1569)
  AUTHORS   Nagao,Y., Kitada,S., Kojima,K., Toh,H., Kuhara,S., Ogishima,T. and
            Ito,A.
  TITLE     Glycine-rich region of mitochondrial processing peptidase
            alpha-subunit is essential for binding and cleavage of the
            precursor proteins
  JOURNAL   J Biol Chem 275 (44), 34552-34556 (2000)
   PUBMED   10942759
REFERENCE   8  (bases 1 to 1569)
  AUTHORS   Kitada,S., Shimokata,K., Niidome,T., Ogishima,T. and Ito,A.
  TITLE     A putative metal-binding site in the beta subunit of rat
            mitochondrial processing peptidase is essential for its catalytic
            activity
  JOURNAL   J Biochem 117 (6), 1148-1150 (1995)
   PUBMED   7490252
REFERENCE   9  (bases 1 to 1569)
  AUTHORS   Paces,V., Rosenberg,L.E., Fenton,W.A. and Kalousek,F.
  TITLE     The beta subunit of the mitochondrial processing peptidase from rat
            liver: cloning and sequencing of a cDNA and comparison with a
            proposed family of metallopeptidases
  JOURNAL   Proc Natl Acad Sci U S A 90 (11), 5355-5358 (1993)
   PUBMED   8506385
REFERENCE   10 (bases 1 to 1569)
  AUTHORS   Kitada,S., Niidome,T., Nagano,T., Ogishima,T. and Ito,A.
  TITLE     Molecular cloning of the smaller subunit(P52) of rat liver
            mitochondrial processing protease
  JOURNAL   Biochem Biophys Res Commun 190 (1), 289-293 (1993)
   PUBMED   8422255
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from L12965.1.
            
            On Aug 31, 2012 this sequence version replaced NM_022395.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: L12965.1, BC078826.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1569              L12965.1           2-1570
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q11"
     gene            1..1569
                     /gene="Pmpcb"
                     /gene_synonym="Mppb"
                     /note="peptidase, mitochondrial processing subunit beta"
                     /db_xref="GeneID:64198"
                     /db_xref="RGD:621297"
     exon            1..115
                     /gene="Pmpcb"
                     /gene_synonym="Mppb"
                     /inference="alignment:Splign:2.1.0"
     CDS             17..1486
                     /gene="Pmpcb"
                     /gene_synonym="Mppb"
                     /EC_number="3.4.24.64"
                     /function="proteolytic removal of mitochondrial leader
                     peptides"
                     /note="mitochondrial-processing peptidase subunit beta;
                     P-52; beta-MPP; peptidase, mitochondrial processing beta
                     subunit; mitochondrial processing peptidase beta subunit;
                     peptidase (mitochondrial processing) beta"
                     /codon_start=1
                     /product="mitochondrial-processing peptidase subunit beta
                     precursor"
                     /protein_id="NP_071790.1"
                     /db_xref="GeneID:64198"
                     /db_xref="RGD:621297"
                     /translation="MAAAAVSRTLLPVAGRRLWGFTRRLPLRAAAAQPLYFGGDRLRS
                     TQAAPQVVLNVPETQVTCLENGLRVASENSGISTCTVGLWIDAGSRYENEKNNGTAHF
                     LEHMAFKGTKKRSQLDLELEIENMGAHLNAYTSREQTVYYAKAFSKDLPRAVEILADI
                     IQNSTLGEAEIERERGVILREMQEVETNLQEVVFDYLHATAYQNTALGRTILGPTENI
                     KSISRKDLVDYITTHYKGPRIVLAAAGGVCHNELLELAKFHFGDSLCAHKGDVPALPP
                     CKFTGSEIRVRDDKMPLAHLAVAIEAVGWTHPDTIRLMVANTLIGNWDRSFGGGMNLS
                     SKLAQLTCHGNLCHSFQSFNTSYTDTGLWGLYMVCEQATVADMLHAVQKEWMRLCTAV
                     SESEVARAKNLLKTNMLLQLDGSTPICEDIGRQMLCYNRRIPIPELEARIDAVDAEMV
                     REVCTKYIYGKSPAIAALGPIERLPDFNQICSNMRWTRD"
     exon            116..256
                     /gene="Pmpcb"
                     /gene_synonym="Mppb"
                     /inference="alignment:Splign:2.1.0"
     exon            257..343
                     /gene="Pmpcb"
                     /gene_synonym="Mppb"
                     /inference="alignment:Splign:2.1.0"
     exon            344..473
                     /gene="Pmpcb"
                     /gene_synonym="Mppb"
                     /inference="alignment:Splign:2.1.0"
     exon            474..672
                     /gene="Pmpcb"
                     /gene_synonym="Mppb"
                     /inference="alignment:Splign:2.1.0"
     exon            673..752
                     /gene="Pmpcb"
                     /gene_synonym="Mppb"
                     /inference="alignment:Splign:2.1.0"
     exon            753..865
                     /gene="Pmpcb"
                     /gene_synonym="Mppb"
                     /inference="alignment:Splign:2.1.0"
     exon            866..1009
                     /gene="Pmpcb"
                     /gene_synonym="Mppb"
                     /inference="alignment:Splign:2.1.0"
     exon            1010..1170
                     /gene="Pmpcb"
                     /gene_synonym="Mppb"
                     /inference="alignment:Splign:2.1.0"
     exon            1171..1256
                     /gene="Pmpcb"
                     /gene_synonym="Mppb"
                     /inference="alignment:Splign:2.1.0"
     exon            1257..1345
                     /gene="Pmpcb"
                     /gene_synonym="Mppb"
                     /inference="alignment:Splign:2.1.0"
     exon            1346..1421
                     /gene="Pmpcb"
                     /gene_synonym="Mppb"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..1569
                     /gene="Pmpcb"
                     /gene_synonym="Mppb"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cctatccaca gcagagatgg cggcggcggc tgtatcccgg acgttgttgc cggtggccgg
       61 acggcgcctg tggggattta cgcgaaggct tccgctccgc gccgccgctg ctcagccatt
      121 gtactttgga ggggaccgac tcagaagtac acaggctgct ccacaagttg ttctgaatgt
      181 tcctgagaca caagtcactt gtttggaaaa tggactcagg gtagcttctg aaaactctgg
      241 gatctcaaca tgcaccgttg ggctgtggat cgatgctggc agtcggtatg agaatgaaaa
      301 gaacaatggc accgctcact ttctggagca catggctttc aagggcacta aaaagaggtc
      361 ccagttagac cttgaacttg agattgagaa catgggtgcc catctcaatg cctatacctc
      421 cagagagcag actgtctact acgccaaagc cttctccaaa gatttgccaa gagctgtaga
      481 aattcttgct gacataattc agaacagtac attgggagag gcagagattg aacgtgagcg
      541 tggagtcatc ctcagagaaa tgcaggaagt tgagaccaac ttgcaagaag ttgtctttga
      601 ttatctgcac gccacagcct atcaaaacac cgcgctcgga cggacaattc tgggaccaac
      661 cgagaacatc aaatctataa gccgtaagga cttagtggac tacataacca cacattataa
      721 gggaccaaga atcgtgctgg ctgctgctgg aggtgtttgc cataacgaac tgctggagtt
      781 agcgaagttt cattttggtg actctttgtg cgcacacaaa ggagatgtac cagctctgcc
      841 tccctgtaaa ttcactggaa gcgagattcg ggtgagggat gacaagatgc cactggcgca
      901 cctcgcagtg gctattgaag cagttggttg gacgcaccca gacacgatcc gtctcatggt
      961 cgcaaacaca ctgataggca actgggaccg ctctttcgga ggaggaatga atttatcgag
     1021 caagctggcc cagctcacgt gccatggcaa tctctgccac agcttccagt ccttcaacac
     1081 ctcctacaca gacacaggat tgtgggggct ctatatggtt tgtgaacaag ccacagttgc
     1141 tgacatgctg cacgctgtac aaaaggaatg gatgcgtctg tgcacagctg ttagtgagag
     1201 tgaggtcgca cgcgccaaaa accttctcaa gacaaacatg ctgctgcagc ttgatgggtc
     1261 aactccgatc tgtgaagaca tcggtaggca gatgctatgc tacaacagga gaatccccat
     1321 ccctgagctt gaggccagga tcgatgctgt ggacgcggag atggttcgag aagtgtgcac
     1381 caagtacatt tatggcaaaa gcccggccat tgctgctctc ggtcctattg agcgcttacc
     1441 agattttaac cagatttgta gtaacatgcg ctggactcgt gactgatttt tgtgatgaat
     1501 tatcctacac atacatattt ataaaactga gacctagaag ttttgaaaga ctgctcatct
     1561 tcaagtgaa
//
