LOCUS       NM_001109235            6657 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus pleckstrin homology domain containing A8
            (Plekha8), mRNA.
ACCESSION   NM_001109235 XM_001060789 XM_575484
VERSION     NM_001109235.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Cao,X., Coskun,U., Rossle,M., Buschhorn,S.B., Grzybek,M.,
            Dafforn,T.R., Lenoir,M., Overduin,M. and Simons,K.
  TITLE     Golgi protein FAPP2 tubulates membranes
  JOURNAL   Proc Natl Acad Sci U S A 106 (50), 21121-21125 (2009)
   PUBMED   19940249
REFERENCE   2  (bases 1 to 6657)
  AUTHORS   D'Angelo,G., Polishchuk,E., Di Tullio,G., Santoro,M., Di Campli,A.,
            Godi,A., West,G., Bielawski,J., Chuang,C.C., van der Spoel,A.C.,
            Platt,F.M., Hannun,Y.A., Polishchuk,R., Mattjus,P. and De
            Matteis,M.A.
  TITLE     Glycosphingolipid synthesis requires FAPP2 transfer of
            glucosylceramide
  JOURNAL   Nature 449 (7158), 62-67 (2007)
   PUBMED   17687330
REFERENCE   3  (bases 1 to 6657)
  AUTHORS   Godi,A., Di Campli,A., Konstantakopoulos,A., Di Tullio,G.,
            Alessi,D.R., Kular,G.S., Daniele,T., Marra,P., Lucocq,J.M. and De
            Matteis,M.A.
  TITLE     FAPPs control Golgi-to-cell-surface membrane traffic by binding to
            ARF and PtdIns(4)P
  JOURNAL   Nat Cell Biol 6 (5), 393-404 (2004)
   PUBMED   15107860
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000004.1.
            
            On Oct 5, 2010 this sequence version replaced NM_001109235.1.
            
            Sequence Note: This RefSeq record was created from genomic sequence
            data to make the sequence consistent with the reference genome
            assembly. The genomic coordinates used for the transcript record
            are predicted based on partial rat transcript alignments and on
            full-length homologous transcript and protein alignments with mouse
            NP_001157833.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643287.11054.1,
                                           SRR26643429.16517.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-207               JAXUCZ010000004.1  85053854-85054060
            208-324             JAXUCZ010000004.1  85074481-85074597
            325-480             JAXUCZ010000004.1  85076511-85076666
            481-605             JAXUCZ010000004.1  85077973-85078097
            606-764             JAXUCZ010000004.1  85080370-85080528
            765-805             JAXUCZ010000004.1  85081316-85081356
            806-966             JAXUCZ010000004.1  85083156-85083316
            967-1123            JAXUCZ010000004.1  85085156-85085312
            1124-1209           JAXUCZ010000004.1  85085630-85085715
            1210-1268           JAXUCZ010000004.1  85089612-85089670
            1269-1399           JAXUCZ010000004.1  85090645-85090775
            1400-1470           JAXUCZ010000004.1  85091441-85091511
            1471-1532           JAXUCZ010000004.1  85093775-85093836
            1533-6657           JAXUCZ010000004.1  85099252-85104376
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q24"
     gene            1..6657
                     /gene="Plekha8"
                     /gene_synonym="FAPP-2; RGD1563940"
                     /note="pleckstrin homology domain containing A8"
                     /db_xref="GeneID:500132"
                     /db_xref="RGD:1563940"
     exon            1..207
                     /gene="Plekha8"
                     /gene_synonym="FAPP-2; RGD1563940"
                     /inference="alignment:Splign:2.1.0"
     CDS             168..1730
                     /gene="Plekha8"
                     /gene_synonym="FAPP-2; RGD1563940"
                     /note="PH domain-containing family A member 8;
                     phosphoinositol 4-phosphate adapter protein 2;
                     phosphatidylinositol-four-phosphate adapter protein 2;
                     pleckstrin homology domain containing, family A
                     (phosphoinositide binding specific) member 8"
                     /codon_start=1
                     /product="pleckstrin homology domain-containing family A
                     member 8"
                     /protein_id="NP_001102705.1"
                     /db_xref="GeneID:500132"
                     /db_xref="RGD:1563940"
                     /translation="MEGVLYKWTNYLSGWQPRWFLLCGGILSYYDSPEDAWKGCKGSI
                     QMAVCEIQVHSVDNTRMDLIIPGEQYFYLKARSVAERQRWLVALGSAKACLTDSRTQK
                     EKEFAENTENLKTKMSELRLYCDLLVQQVDKTKEVATAGVADSEEGIDVGTLLRSTCN
                     TFLKTLEECMQIANAAFTSELLYHTPPGSPQLAVLKSSKMKHPIIPIHNSLERPIELN
                     SCENGSLSVEVNGDEELLMKTKKSSLYLKSTKADCNISSEENTDGNTTVQGERMKEDG
                     EENLESHDRDLAQPGSDSVCSPESPWEDSEEVIPTFFSTMNTSFSDIELLEDSGIPTE
                     AFLASCYAVVPVLDKLGPTVFAPVKMDLVGNIKKVNQKYITNKEEFTTLQKIVLHEVE
                     ADVARVRNSATEALLWLKRGLKFLKGFLTEVKNGEKDIQTALNNAYGKTLRQHHGWVV
                     RGVFALALRAAPSYEDFVAALTIKEGDHQKEAFSAGMQRDLSLYLPAMEKQLAILDTL
                     YEIHGLESDEVV"
     exon            208..324
                     /gene="Plekha8"
                     /gene_synonym="FAPP-2; RGD1563940"
                     /inference="alignment:Splign:2.1.0"
     exon            325..480
                     /gene="Plekha8"
                     /gene_synonym="FAPP-2; RGD1563940"
                     /inference="alignment:Splign:2.1.0"
     exon            481..605
                     /gene="Plekha8"
                     /gene_synonym="FAPP-2; RGD1563940"
                     /inference="alignment:Splign:2.1.0"
     exon            606..764
                     /gene="Plekha8"
                     /gene_synonym="FAPP-2; RGD1563940"
                     /inference="alignment:Splign:2.1.0"
     exon            765..805
                     /gene="Plekha8"
                     /gene_synonym="FAPP-2; RGD1563940"
                     /inference="alignment:Splign:2.1.0"
     exon            806..966
                     /gene="Plekha8"
                     /gene_synonym="FAPP-2; RGD1563940"
                     /inference="alignment:Splign:2.1.0"
     exon            967..1123
                     /gene="Plekha8"
                     /gene_synonym="FAPP-2; RGD1563940"
                     /inference="alignment:Splign:2.1.0"
     exon            1124..1209
                     /gene="Plekha8"
                     /gene_synonym="FAPP-2; RGD1563940"
                     /inference="alignment:Splign:2.1.0"
     exon            1210..1268
                     /gene="Plekha8"
                     /gene_synonym="FAPP-2; RGD1563940"
                     /inference="alignment:Splign:2.1.0"
     exon            1269..1399
                     /gene="Plekha8"
                     /gene_synonym="FAPP-2; RGD1563940"
                     /inference="alignment:Splign:2.1.0"
     exon            1400..1470
                     /gene="Plekha8"
                     /gene_synonym="FAPP-2; RGD1563940"
                     /inference="alignment:Splign:2.1.0"
     exon            1471..1532
                     /gene="Plekha8"
                     /gene_synonym="FAPP-2; RGD1563940"
                     /inference="alignment:Splign:2.1.0"
     exon            1533..6657
                     /gene="Plekha8"
                     /gene_synonym="FAPP-2; RGD1563940"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cggccgctct gccgcgtcca caccgaccgc gccaagcgca ggcgcagccc cgggacccta
       61 agctggtgct cctaggctgt cgcggtgacc ccgcccccgg gcccaggatg tgaggcaggc
      121 ccggtgtccc cgcggcgggc cggggcggtg aggggcccgc aggcgccatg gagggcgtgc
      181 tgtacaagtg gaccaactac ctgagcggtt ggcagcctcg atggttcctt ctctgtgggg
      241 gaatactgtc ctattacgat tctcctgaag atgcctggaa gggttgcaaa gggagcatcc
      301 agatggcagt ctgtgagatt caagttcatt ccgtggataa cactcgtatg gacctcatca
      361 tccctgggga acagtatttc tacttaaaag ccagaagtgt ggcagagaga cagcgatggc
      421 tggtggctct gggatctgcc aaggcctgcc tgactgatag taggactcaa aaggaaaaag
      481 aatttgcaga aaacactgaa aacctgaaaa ccaaaatgtc ggaactaagg ctatactgtg
      541 acctccttgt tcaacaagta gataaaacaa aagaagtggc cacagccggt gttgctgact
      601 ctgaggaggg aatcgatgtg ggaactctgc tgagatcaac ctgcaacact ttcctgaaga
      661 ccttggagga gtgcatgcag attgcaaatg cagcctttac ctctgaactg ctctaccata
      721 cacccccagg gtcccctcaa ctagcggtgc tcaagtccag caagatgaaa catcctatca
      781 taccaattca taattcattg gaaaggccga tagagttaaa cagttgtgaa aatggatcgc
      841 taagtgtaga agtaaatggt gatgaagaac tcctaatgaa aaccaagaag agttccttat
      901 atttgaaatc cacaaaggca gattgcaaca tctccagtga agaaaacaca gatggaaata
      961 cgacagtcca gggtgagaga atgaaggaag atggagagga aaacctggag agtcatgaca
     1021 gggatctggc acagccggga tcagactcag tttgctcgcc agaatctccc tgggaagaca
     1081 gtgaagaagt tattccaact ttcttcagta ctatgaatac cagctttagt gacattgagc
     1141 ttctggaaga cagtggcatt cccacagaag cattcttggc atcttgttat gcagtggttc
     1201 cagtactaga taaacttggt cccaccgtgt ttgctcctgt taagatggat cttgttggaa
     1261 atattaagaa ggtaaatcag aagtacataa ccaacaagga agagtttacc accctccaga
     1321 agatagtgct gcacgaggtg gaagccgatg tggctcgggt taggaactcg gcaactgaag
     1381 ccctcttgtg gctgaagaga ggtctcaaat ttctaaaggg atttttgaca gaagtgaaga
     1441 atggggaaaa ggacatccag acagcactga ataatgcata tggcaaaaca ttacggcaac
     1501 accatggctg ggtagttcga ggggtttttg cgttagcact gagggcagca ccatcctatg
     1561 aggactttgt ggctgcacta accataaaag aaggcgacca tcagaaagaa gctttcagtg
     1621 ctgggatgca gagggacctc agcctttacc tccctgctat ggaaaagcag ctggccatac
     1681 tggacacgtt atatgagatc cacgggctgg aatctgacga ggtggtgtga cagccacagg
     1741 acagcacctc ctaacatcag ggaataaagt ctgtaaagtg ttgtgttgcc ctagttgatt
     1801 ttcagcaaca gctttgatac cctccagcac ctttctgtag ggaggtggac atgaggaaga
     1861 cctaatgctt ttatttagaa ttgttttaat gcatatgtgt tccatgtgtt taaaaatcaa
     1921 ggtgctaaat atttcaacag gccaactgga aaccagagca tcgctgttcc agacctgagt
     1981 ggtaggcagt aagagcaaac tgacagatga atttgttggg atcacacctg tattgttctg
     2041 tttcagttac tagttttaat gggcacctaa acccactcag tatgcagggc tataatacaa
     2101 agtcagtgat gagaatagct tagaggctga ctgggcatca gagggagtgt aagccccttc
     2161 attgaaggac ttgctgtgta agagtttgtt attcctagac tttcttgagt ggatcccttc
     2221 tccgttgctc tgcagatggg aagggctgtg tagtgcagac atgtgggcag ttgtgggtaa
     2281 acactgacaa ggactgccat gcctattgtc aggtggaacg gtggctcaag aggatgatct
     2341 cagcccatgc tggagaacag aatagaaagg tgcagtaagt catttgggtg acacatgcca
     2401 ctctttgcca atacaataca tttcccgtgt gttatctgag ttggacatca tagcaaaaca
     2461 gcaactgtaa aagagtattc atcctgcttc ccaagtaggg cttacagtag ggcctgctca
     2521 cagtcatgga ggatcttgtt tctgaggact ttatttttat ttatttattt atttattttg
     2581 cctgctttat taactctctt cagcaaaaga gccaggtagc ttccaaatgt gatctagatc
     2641 acctctgtaa taggtgatca catgacgtgc tatttcaaag aagagagtcc agagtggaat
     2701 gtccctgggg ataacctttc taggtgacac actcttgtaa acaaggctga agagccaaat
     2761 tcccatttga actctagttt acagaattcc caggcttctg cagaaaccaa ataaaacacc
     2821 ctccttccag aggaaggggc catcattcaa gtaacataat taaatggaga gtgaatagac
     2881 cagcagctgg tgtaacagct tgtggcattc ttctataaag agtgacagga tgccccatct
     2941 cccagaaatg gaccaagctg cctagagtgt gcacagggaa gaagccatga atgccttccc
     3001 cagtagaggc tacttccccc tagcaacaag aagagtcact ctagcataag cagtcccctg
     3061 aagaaagtga gctctttgcc aagcacaggt aaagggagtg gctgtgcacg ctgcttttgt
     3121 tgctgtatag actagggcac tgcagagaac ttttcaggga aaggtactaa tttgtctttt
     3181 gtctgtcttt aaacaagtgg gtattcactc gaattaatgc agcagtagag tgggccacag
     3241 aggcccctgc agtgccagct actcagaagt atgagggcga agtattattt gagcccagga
     3301 tttggaggtt aaccaaaaca acatagcaag accttgcctc ttaaaaatac ttttaataaa
     3361 attttaagcc aagcaagcat tttgctgaca tgagaagttc tagaaattaa acctaggaaa
     3421 gggaggactg ggctggactt aacagtgtgc tactttgctc ttgcaggagt ttcatatgga
     3481 tcctcttctc tggctaatgg aaatggggga cagggccagg gcatagctta gacctcctgt
     3541 cctccacaca gcctaggaca ggaaaggaaa agtagccaac ctgcttgtct ctgctaccag
     3601 gaagtcggca ttaagaatgg ctgtgagcag agccgtaggt tcctctggat ctctagtctg
     3661 ggactggcac tctgagagca ttttagtcca ggtcctcact aaggccctga gtgcacgtgt
     3721 aaggagagct gacttgcagg gcagtctggg tttgtaagtt catcccacct gaattagcag
     3781 ttcttggttt ctcattttag ttaaagctaa tttatttcat ctgattcagt tactttctaa
     3841 gaaatgaaaa gaagaacctg aaagtgctcc ctggagctgc ctcccccaca agagccccag
     3901 tatccctgag ccaccctcac cctgacagat gaaggactgg ttggtttgtt gacagaatgt
     3961 ttaccttatc ttcattgcta aaagtttttg gatgttttcc tagcataatc ttgagactag
     4021 ggaaatgtct tcctagagag cagcagaatt gaatgagcgg cactggctga gggcttacgg
     4081 agctcagccc caacaccatc ccatggtttg cccttcaccc tcaccccatg acgcttggct
     4141 ccacgtgggc tgacatcagt atgtgttgaa ctgaacaaag gctttttaat tgtgataagc
     4201 cttagtcttt taaaaataga tctgattata actaaaacct actgacagat gctgccttgg
     4261 aggaatatct ttaagggtag aaaccatctt ataattgagg cagagttgac agctcttgac
     4321 ccaggctgga agggtaccgt aagcacataa tggcttgttg ctatgtccaa gtcattataa
     4381 gcatgaacgc acccgtagag tcacttacca cagtgggtaa catctgagct cctcttctca
     4441 gccttgcagc ttttctctgc tactgagcat tgctagcagt ggaccccctg taccaccccc
     4501 catcagcttg ccacagtggt ttgctctccc agctcttgtt tccccttctt cagcggcagc
     4561 agcagcccac ctggtccagg atctgttata cacctagcca aaatgccagc gcttagggca
     4621 gacgccatgt tcacatttag ctgtaaggta agggaaagtc agcatgggag agctgagtgc
     4681 cacaggaaaa cgcaccctcg gctttgattg ctccatggac agttagtgca gtccgcttct
     4741 gtctgtggag tatttccagg tgcaggaagc gtctgatgcg gtccacaaac acgggacact
     4801 tggttaaact tgaagtgaga agagctgaag aactcttaac tagtccgact gtaacagtca
     4861 ttttacaggg agagatttca gacaaagcaa cagaagtcag agggttgtga gcaatacaag
     4921 atataaggca ttttggggac atttaaaaga attttttaaa tgtgtttcct actagaccac
     4981 tatgtccttc aggcatggag ctatgtcatg ttgtgtttct gaatcatcaa ttcaaaccac
     5041 tgcttctgat tttagtcaca ggtttacaac ggttcaaatc cctaccctca tgtttcattt
     5101 cactaccaat catttttaaa ttctaaggtg tctggataga agagttctga gtaactggaa
     5161 tacgtgttgg gccattactc tgacatctaa tgcatttggg cagagaaagc cagacatggg
     5221 atgctttgtg acactgtgca gactacatgg tcatgtgcat cagacccatc ctgtgatgtg
     5281 cacatgaagt gatatgacat atgtgttcta cttccatgtg tccctttgta gcctgcagta
     5341 gcttactcag gagttacaca atgacataaa tatccgccag ctgtcgagtg gcatttgaag
     5401 actttatcca tgacaaggtt atagtggcat tacattagtt gagggtcctt cactgcacac
     5461 tggtccttcc ctgtgaattc tcccagtcca accctgtcta gactctgctg cagaccattt
     5521 gcatgatttc ttgctgtaca gctgcatttg ggaactctag ggcatgacca cccagtcact
     5581 ggtaatgcct ctggtacaga ggtcgcatgc attgatcctc ttctccattg tctatatatg
     5641 gcctttaatg gaaaggaaaa aatgccacta ccctgcattg cagttttcag aattcttgta
     5701 aattgtgaac ccttgagtca cttcttcagg gtacaaagtg tttctagtca agctgtgtgg
     5761 ggacatcctg cttgttttaa atgtcttata tatttacagt atggtcagta tcttcccttt
     5821 acacatagcc ttgggcaagg tggtcccctc accctgaggt gggtggttcc atcagcctta
     5881 ggacgggtgg cttcattaca caactggtct cattcccaga gagccccaag tgtccattca
     5941 gatgtgtagg tggagttggt gaagagaagg gtgatctaga tggctgatga gccagcctca
     6001 tggcatgggg taccggatga ttcctgtgtg catgagaact tcactagaat gtcattaaac
     6061 agcccaacta cctcaagtga aattggctgt ggacaatctg tgtttgatga gaaatgtgtt
     6121 tggagaaact tattctggtt aataagttga atagtgcagt gtctctatga agaagaaaca
     6181 tgttatacca gatgccaaag gctaaactat acataaattc tctcagatcc atttctatgt
     6241 taatgtttaa ttcctcagtc tgtatcctca tgaacatttg tagctttgta gagccatgtg
     6301 ctttgggtat ctgtcacaga atcacctctt gtccctcagt attttgaatg aacatcataa
     6361 tgctgatagg aactaagtta aactacgaaa gtgtcaaaca acttgtggct tcttaattta
     6421 atggaccttt acttagaaat gtattggagc cttttaggac caattgttga gtgaaaatct
     6481 tgtgtgcaaa cgtgtctgct tttctgtcca agttgttatt cctgtatttc atgaacagtg
     6541 gggcaggggt aagggtttta ctttctttgg agaatgtttg aaaacatctg tcagattttt
     6601 atattcatta gttacaataa acatattttt agagtatttt attaaatgtt cttcaaa
//
