LOCUS       NM_001025750            1671 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Rattus norvegicus pleckstrin (Plek), mRNA.
ACCESSION   NM_001025750 XM_344267
VERSION     NM_001025750.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Frobel,J., Cadeddu,R.P., Hartwig,S., Bruns,I., Wilk,C.M.,
            Kundgen,A., Fischer,J.C., Schroeder,T., Steidl,U.G., Germing,U.,
            Lehr,S., Haas,R. and Czibere,A.
  TITLE     Platelet proteome analysis reveals integrin-dependent aggregation
            defects in patients with myelodysplastic syndromes
  JOURNAL   Mol Cell Proteomics 12 (5), 1272-1280 (2013)
   PUBMED   23382103
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   3  (bases 1 to 1671)
  AUTHORS   Lian,L., Wang,Y., Flick,M., Choi,J., Scott,E.W., Degen,J.,
            Lemmon,M.A. and Abrams,C.S.
  TITLE     Loss of pleckstrin defines a novel pathway for PKC-mediated
            exocytosis
  JOURNAL   Blood 113 (15), 3577-3584 (2009)
   PUBMED   19190246
REFERENCE   4  (bases 1 to 1671)
  AUTHORS   Edlich,C., Stier,G., Simon,B., Sattler,M. and Muhle-Goll,C.
  TITLE     Structure and phosphatidylinositol-(3,4)-bisphosphate binding of
            the C-terminal PH domain of human pleckstrin
  JOURNAL   Structure 13 (2), 277-286 (2005)
   PUBMED   15698571
REFERENCE   5  (bases 1 to 1671)
  AUTHORS   Roll,R.L., Bauman,E.M., Bennett,J.S. and Abrams,C.S.
  TITLE     Phosphorylated pleckstrin induces cell spreading via an
            integrin-dependent pathway
  JOURNAL   J Cell Biol 150 (6), 1461-1466 (2000)
   PUBMED   10995449
REFERENCE   6  (bases 1 to 1671)
  AUTHORS   McDermott,A.M. and Haslam,R.J.
  TITLE     Chemical cross-linking of pleckstrin in human platelets: evidence
            for oligomerization of the protein and its dissociation by protein
            kinase C
  JOURNAL   Biochem J 317 (Pt 1) (Pt 1), 119-124 (1996)
   PUBMED   8694752
REFERENCE   7  (bases 1 to 1671)
  AUTHORS   Craig,K.L. and Harley,C.B.
  TITLE     Phosphorylation of human pleckstrin on Ser-113 and Ser-117 by
            protein kinase C
  JOURNAL   Biochem J 314 (Pt 3) (Pt 3), 937-942 (1996)
   PUBMED   8615792
REFERENCE   8  (bases 1 to 1671)
  AUTHORS   Abrams,C.S., Zhao,W., Belmonte,E. and Brass,L.F.
  TITLE     Protein kinase C regulates pleckstrin by phosphorylation of sites
            adjacent to the N-terminal pleckstrin homology domain
  JOURNAL   J Biol Chem 270 (40), 23317-23321 (1995)
   PUBMED   7559487
REFERENCE   9  (bases 1 to 1671)
  AUTHORS   Abrams,C.S., Wu,H., Zhao,W., Belmonte,E., White,D. and Brass,L.F.
  TITLE     Pleckstrin inhibits phosphoinositide hydrolysis initiated by
            G-protein-coupled and growth factor receptors. A role for
            pleckstrin's PH domains
  JOURNAL   J Biol Chem 270 (24), 14485-14492 (1995)
   PUBMED   7782310
REFERENCE   10 (bases 1 to 1671)
  AUTHORS   Tyers,M., Haslam,R.J., Rachubinski,R.A. and Harley,C.B.
  TITLE     Molecular analysis of pleckstrin: the major protein kinase C
            substrate of platelets
  JOURNAL   J Cell Biochem 40 (2), 133-145 (1989)
   PUBMED   2768345
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC098846.1.
            
            On Jul 22, 2005 this sequence version replaced XM_344267.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC098846.1, FQ225547.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN06680436, SAMN09767679
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="14"
                     /map="14q22"
     gene            1..1671
                     /gene="Plek"
                     /note="pleckstrin"
                     /db_xref="GeneID:364206"
                     /db_xref="RGD:1308269"
     exon            1..251
                     /gene="Plek"
                     /inference="alignment:Splign:2.1.0"
     CDS             210..1262
                     /gene="Plek"
                     /codon_start=1
                     /product="pleckstrin"
                     /protein_id="NP_001020921.1"
                     /db_xref="GeneID:364206"
                     /db_xref="RGD:1308269"
                     /translation="MEPKRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNNP
                     KGMIPLKGSTLTSPCQDFGKRMFVLKITTTKQQDHFFQAAYLEERDAWVRDIKKAIKC
                     IEGGQKFARKSTRRSIRLPETIDLGALYLSMKDPEKGIKELNLEKDKKVFNHCFTGSG
                     VIDWLVSNKLVRNRQEGLMISASLLSEGYLQPASDLSKNAADGIAENPFLDNPDAFYY
                     FPDSGFFCEENSSDDDIILKEEFRGIIIKQGCLLKQGHRRKNWKVRKFILREDPAYLH
                     YYDPAGGEDPLGAIHLRGCVVTSVESNPDGKKSDEENLFEIITADEVHYYMQAATAKE
                     RTEWIKAIQVASRTGK"
     exon            252..407
                     /gene="Plek"
                     /inference="alignment:Splign:2.1.0"
     exon            408..589
                     /gene="Plek"
                     /inference="alignment:Splign:2.1.0"
     exon            590..681
                     /gene="Plek"
                     /inference="alignment:Splign:2.1.0"
     exon            682..866
                     /gene="Plek"
                     /inference="alignment:Splign:2.1.0"
     exon            867..971
                     /gene="Plek"
                     /inference="alignment:Splign:2.1.0"
     exon            972..1055
                     /gene="Plek"
                     /inference="alignment:Splign:2.1.0"
     exon            1056..1125
                     /gene="Plek"
                     /inference="alignment:Splign:2.1.0"
     exon            1126..1258
                     /gene="Plek"
                     /inference="alignment:Splign:2.1.0"
     exon            1259..1648
                     /gene="Plek"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggctattcag ctatctagtt cctttttctg acttgtcacc ccagtgaggg gaaaaggatg
       61 tcatcatgca gtcaccgccg gcagaagaga aattgcgaaa gagacctcac tgtataaagg
      121 gcagcccaca agcgcctagg tctccagagg agcctgagct cctcagagat cgtgtctgaa
      181 gaggggacct gtgcgactgt ctaccaggca tggaaccaaa gcgcatcagg gagggctacc
      241 ttgtgaagaa gggaagtgtg tttaacacct ggaaacccat gtgggttgta ctgctagaag
      301 acggaattga gttttataag aagaaaagtg acaacaaccc caaggggatg atcccgctga
      361 aaggaagtac tctaaccagc ccctgtcaag actttggcaa acggatgttc gtgttaaaaa
      421 tcactacaac caaacagcag gaccacttct tccaggcagc ctacctggag gagagagatg
      481 cttgggttcg ggacatcaag aaagccatta aatgcattga aggaggccag aagtttgcaa
      541 gaaaatccac aaggaggtcc attcgtctgc cagagacaat tgacttgggg gctttatatt
      601 tgtctatgaa agaccctgag aaaggaatca aggaactgaa tctagagaag gacaagaaag
      661 tttttaatca ctgcttcaca ggtagtggtg tcattgactg gctggtttct aacaagctag
      721 ttcggaaccg ccaggaaggc ctcatgatct ccgcctccct gctcagtgaa gggtacctgc
      781 agcctgctag cgatctgtcc aagaatgcag cagatggaat agctgaaaac cctttcctgg
      841 acaaccctga tgccttctac tacttcccag acagtggatt cttctgtgag gagaactcca
      901 gtgacgatga catcattctg aaagaagaat tcagaggcat tatcattaag cagggatgtt
      961 tattgaagca ggggcaccgg aggaaaaact ggaaagtgag gaagttcatt ctgagagaag
     1021 acccggccta cttgcactac tatgaccctg ctgggggtga agatcccctg ggagcaatcc
     1081 acttgagagg ctgtgtggtg acttccgtag agagcaaccc cgatggtaag aagagtgatg
     1141 aggagaacct ctttgagatc atcacagcag acgaagttca ttattacatg caggcagcca
     1201 ctgccaagga gcgtactgag tggataaaag ccatccaggt ggcctcacgg actgggaaat
     1261 gaggatacca agaagcattg acgcgaatcc gactggccaa gagtctacaa ctaggaaaat
     1321 agagaggaat tcaagaccag actttgaact ttcagttggc gttctgtctt cgccttcctc
     1381 agatgggtcc cagctgtagc attattgttt atttaatatt cactgaagca agaattctca
     1441 ctttgtatgt ttgttcaccg catcccggca ctcaaagaaa acaatcttgt gcctgtataa
     1501 cttgccaggg gaactctgga gacctcacgg atattccaga aagtctcaag gctggaggag
     1561 gacgagatgt tcgttctttt gtattctgag ttctcccacc tgttttcttc tcacctgaat
     1621 aaaacaattt ctgtgagact ctacacaaaa aaaaaaaaaa aaaaaaaaaa a
//
