LOCUS       NM_001012234            2236 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus phospholipase C, zeta 1 (Plcz1), mRNA.
ACCESSION   NM_001012234 XM_006237597 XM_575711
VERSION     NM_001012234.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Nomikos,M., Stamatiadis,P., Sanders,J.R., Beck,K., Calver,B.L.,
            Buntwal,L., Lofty,M., Sideratou,Z., Swann,K. and Lai,F.A.
  TITLE     Male infertility-linked point mutation reveals a vital binding role
            for the C2 domain of sperm PLCzeta
  JOURNAL   Biochem J 474 (6), 1003-1016 (2017)
   PUBMED   28270562
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Escoffier,J., Lee,H.C., Yassine,S., Zouari,R., Martinez,G.,
            Karaouzene,T., Coutton,C., Kherraf,Z.E., Halouani,L., Triki,C.,
            Nef,S., Thierry-Mieg,N., Savinov,S.N., Fissore,R., Ray,P.F. and
            Arnoult,C.
  TITLE     Homozygous mutation of PLCZ1 leads to defective human oocyte
            activation and infertility that is not rescued by the WW-binding
            protein PAWP
  JOURNAL   Hum Mol Genet 25 (5), 878-891 (2016)
   PUBMED   26721930
REFERENCE   3  (bases 1 to 2236)
  AUTHORS   Nomikos,M., Sanders,J.R., Kashir,J., Sanusi,R., Buntwal,L.,
            Love,D., Ashley,P., Sanders,D., Knaggs,P., Bunkheila,A., Swann,K.
            and Lai,F.A.
  TITLE     Functional disparity between human PAWP and PLCzeta in the
            generation of Ca2+ oscillations for oocyte activation
  JOURNAL   Mol Hum Reprod 21 (9), 702-710 (2015)
   PUBMED   26116451
REFERENCE   4  (bases 1 to 2236)
  AUTHORS   Ito,M., Shikano,T., Oda,S., Horiguchi,T., Tanimoto,S., Awaji,T.,
            Mitani,H. and Miyazaki,S.
  TITLE     Difference in Ca2+ oscillation-inducing activity and nuclear
            translocation ability of PLCZ1, an egg-activating sperm factor
            candidate, between mouse, rat, human, and medaka fish
  JOURNAL   Biol Reprod 78 (6), 1081-1090 (2008)
   PUBMED   18322275
  REMARK    GeneRIF: All PLCZ1 proteins including fish could induce Ca(2+)
            oscillations in mouse eggs, but the activity was variable in the
            order of human >> mouse > medaka >> rat, estimated from minimal RNA
            concentration to induce Ca(2+) spikes.
REFERENCE   5  (bases 1 to 2236)
  AUTHORS   Kouchi,Z., Fukami,K., Shikano,T., Oda,S., Nakamura,Y., Takenawa,T.
            and Miyazaki,S.
  TITLE     Recombinant phospholipase Czeta has high Ca2+ sensitivity and
            induces Ca2+ oscillations in mouse eggs
  JOURNAL   J Biol Chem 279 (11), 10408-10412 (2004)
   PUBMED   14701816
REFERENCE   6  (bases 1 to 2236)
  AUTHORS   Saunders,C.M., Larman,M.G., Parrington,J., Cox,L.J., Royse,J.,
            Blayney,L.M., Swann,K. and Lai,F.A.
  TITLE     PLC zeta: a sperm-specific trigger of Ca(2+) oscillations in eggs
            and embryo development
  JOURNAL   Development 129 (15), 3533-3544 (2002)
   PUBMED   12117804
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000004.1.
            
            On or before Oct 7, 2022 this sequence version replaced
            XM_006237597.4, NM_001012234.1.
            
            ##Evidence-Data-START##
            CDS exon combination :: AY885259.1 [ECO:0000331]
            RNAseq introns       :: partial sample support SAMEA5760383,
                                    SAMEA5760389 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-69                JAXUCZ010000004.1  174660222-174660290 c
            70-224              JAXUCZ010000004.1  174659500-174659654 c
            225-348             JAXUCZ010000004.1  174658572-174658695 c
            349-580             JAXUCZ010000004.1  174644951-174645182 c
            581-782             JAXUCZ010000004.1  174639277-174639478 c
            783-927             JAXUCZ010000004.1  174631708-174631852 c
            928-1077            JAXUCZ010000004.1  174629077-174629226 c
            1078-1317           JAXUCZ010000004.1  174625698-174625937 c
            1318-1474           JAXUCZ010000004.1  174622618-174622774 c
            1475-1591           JAXUCZ010000004.1  174618646-174618762 c
            1592-1764           JAXUCZ010000004.1  174616854-174617026 c
            1765-1891           JAXUCZ010000004.1  174607533-174607659 c
            1892-2041           JAXUCZ010000004.1  174605259-174605408 c
            2042-2236           JAXUCZ010000004.1  174604281-174604475 c
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q44"
     gene            1..2236
                     /gene="Plcz1"
                     /note="phospholipase C, zeta 1"
                     /db_xref="GeneID:497197"
                     /db_xref="RGD:1359567"
     exon            1..69
                     /gene="Plcz1"
                     /inference="alignment:Splign:2.1.0"
     exon            70..224
                     /gene="Plcz1"
                     /inference="alignment:Splign:2.1.0"
     CDS             193..2130
                     /gene="Plcz1"
                     /EC_number="3.1.4.11"
                     /note="phosphoinositide phospholipase C-zeta-1;
                     PLC-zeta-1"
                     /codon_start=1
                     /product="1-phosphatidylinositol 4,5-bisphosphate
                     phosphodiesterase zeta-1"
                     /protein_id="NP_001012234.1"
                     /db_xref="GeneID:497197"
                     /db_xref="RGD:1359567"
                     /translation="MESHYELAEARWFMSKIQDYFRGGKISAGITHKLLEKLDFPCHF
                     AHVKRIFKENDRHNQGRITTEDFRTIYRCIVHREEIVEIFNTYTENRKILPEDSLIEF
                     LTQEQYEMEMDESSSVEIIQKYEPIAEVKNERQMSIEGFARYMFSSECLLFKETCNTV
                     YQDMNKPLNDYYISSSHNTYLISDQILGPSDIWGYISALVKGCRCLEIDCWDGAQNEP
                     IVYHGYTLTSKLLFKTVIQAINKYAFVTSDYPVVLSLENHCSPGQQEVMTDILQSTFG
                     DFLLSDILDEFPDSLPSPEALKFKILVKNKKVGTLSETRERLGTDKRGIALDLEEEIY
                     ENEDEDSGKEPETWDDFLSRVKEEQEADPSTLSGIADAKKKIRKLRVALALSDLVIYT
                     KAEKFRNFQYSRVYQQFNETTSMGESRARKLSKLRAHEFIFHTAAFITRVYPKFTRAD
                     SSNFNPQEFWNVGCQMVALNFQTPGLPMDLQNGKFLDNGGSGYVLKPDFLRDTTLGFN
                     PNEPEGDGHPVTLTIRLISGIQLPVNVPSNTSDIIVIIEVYGVPNDHMKQQSRAVKNN
                     AFSPRWNETFTFLIQVPELALIRFVVETQGFLSGNELLGQYTLPVLCMNKGYRRVPLF
                     SKSGANLEPSSLFIYVWYYRE"
     exon            225..348
                     /gene="Plcz1"
                     /inference="alignment:Splign:2.1.0"
     exon            349..580
                     /gene="Plcz1"
                     /inference="alignment:Splign:2.1.0"
     exon            581..782
                     /gene="Plcz1"
                     /inference="alignment:Splign:2.1.0"
     exon            783..927
                     /gene="Plcz1"
                     /inference="alignment:Splign:2.1.0"
     exon            928..1077
                     /gene="Plcz1"
                     /inference="alignment:Splign:2.1.0"
     exon            1078..1317
                     /gene="Plcz1"
                     /inference="alignment:Splign:2.1.0"
     exon            1318..1474
                     /gene="Plcz1"
                     /inference="alignment:Splign:2.1.0"
     exon            1475..1591
                     /gene="Plcz1"
                     /inference="alignment:Splign:2.1.0"
     exon            1592..1764
                     /gene="Plcz1"
                     /inference="alignment:Splign:2.1.0"
     exon            1765..1891
                     /gene="Plcz1"
                     /inference="alignment:Splign:2.1.0"
     exon            1892..2041
                     /gene="Plcz1"
                     /inference="alignment:Splign:2.1.0"
     exon            2042..2236
                     /gene="Plcz1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agccaagaag ctatagcaac tgtgaaggtt ttaagaactt agcttctgga caagtcttct
       61 tcataagcga aaggaggcag cggtgagaac agctgatgac tatcgtaaaa aggcagagtt
      121 acttctaaga ttaagtgaca ccttagacga agagccctct atgggaggac acgcgaccca
      181 gatcatgaaa tcatggagag ccattatgag ctcgcagaag caagatggtt tatgtcaaag
      241 attcaggatt attttagagg tggaaaaatc agcgctggaa ttactcacaa actgctcgag
      301 aaacttgatt tcccatgcca ttttgctcat gtgaaacgta tttttaagga aaatgacaga
      361 cataaccaag gaagaatcac caccgaagat tttagaacca tctatcggtg tattgtacat
      421 agagaagaga tcgttgagat tttcaacacg tatactgaaa acaggaaaat tctccccgag
      481 gacagtctga ttgaatttct aacccaagag cagtatgaaa tggagatgga tgagtccagt
      541 tcagtggaga tcatccagaa gtacgagccc attgcagaag taaagaacga gcggcagatg
      601 tcaattgaag gttttgcaag atacatgttt tcttcagaat gtctactgtt taaagagacg
      661 tgtaacacag tgtaccaaga tatgaataag ccactaaatg attactatat ttcatcgtct
      721 cacaacacat atttgatatc tgatcaaata ttgggaccaa gtgacatttg gggatatata
      781 agtgccctcg tgaaaggctg ccgctgtctg gaaattgact gctgggatgg agcacaaaat
      841 gaacccattg tgtaccatgg ctacactctc accagcaagc ttctcttcaa aaccgttatc
      901 caagcaataa acaagtacgc cttcgtgacg tctgattacc cagtggtgct gtccttagag
      961 aatcactgct cccctggtca acaggaagtg atgaccgaca ttctgcagag tacctttgga
     1021 gactttctgc tctcagacat acttgacgag tttccagaca gtttgccatc tccagaggca
     1081 ctgaaattta aaatattagt gaaaaataag aaagttggaa ccttatctga aacccgcgag
     1141 aggctgggga ctgacaaaag gggcatagcg ctagacttgg aagaagaaat ctatgaaaat
     1201 gaagacgaag actcaggaaa ggagccagaa acgtgggatg atttcctgtc acgggttaag
     1261 gaggagcagg aggcagaccc ctcaacgttg agcggaatag cagatgccaa gaaaaagatc
     1321 aggaagctaa gagtagctct ggccttatct gatcttgtca tttataccaa agctgagaag
     1381 ttccgaaact tccaatattc aagagtctat cagcagttta atgagaccac ttctatggga
     1441 gagtctcgag ctcgaaaact ttcaaaattg agagcccatg agtttatttt ccacactgca
     1501 gcgttcatca ccagagtgta ccccaagttc acgagagcag actcttctaa ttttaatcct
     1561 caagagtttt ggaatgtggg ctgtcagatg gtggccttga attttcaaac ccctggactg
     1621 cctatggatt tgcaaaacgg gaaatttttg gataatggag gctctggata tgttttgaag
     1681 ccagactttc ttagagacac aactttgggc tttaacccaa atgaaccaga aggagatggc
     1741 catccggtta ccctcacgat ccgactcatc agtgggatcc agttgcctgt taacgtgccc
     1801 tcaaatacat ctgacataat agtgatcata gaagtctacg gtgtcccaaa cgaccacatg
     1861 aagcagcaga gtcgtgccgt taagaacaat gcttttagtc caaggtggaa tgaaacattc
     1921 acatttctta ttcaagtgcc agaattggca ctgatacgtt tcgttgttga aactcaaggc
     1981 ttcctatcgg gaaatgaatt acttgggcag tacactttac ccgttctttg catgaacaaa
     2041 ggttatcgtc gcgttcctct gttttccaaa tccggtgcga accttgaacc ttcctctctg
     2101 tttatttacg tttggtacta cagagagtga caacgtgtaa cggaagcgtt agttacacct
     2161 cacagtaaac agtccaaagg aatgttggca cgccctcttt tgttgaccgt aaaataaaga
     2221 ctcattaagt ttgaaa
//
