LOCUS       NM_053758               8355 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus phospholipase C, epsilon 1 (Plce1), mRNA.
ACCESSION   NM_053758
VERSION     NM_053758.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 8355)
  AUTHORS   Xin-Guo, Yong-Yang, Ma,J.Q., Xi-Zou, Li,L.S., Li,Y.H., Hu,Y.Z. and
            Rui-Liu.
  TITLE     PLCepsilon1 mediates one-lung ventilation injury by regulating the
            p38/RhoA/NFkappaB activation loop
  JOURNAL   Mol Immunol 133, 135-145 (2021)
   PUBMED   33662817
  REMARK    GeneRIF: PLCepsilon1 mediates one-lung ventilation injury by
            regulating the p38/RhoA/NFkappaB activation loop.
REFERENCE   2  (bases 1 to 8355)
  AUTHORS   Rugema,N.Y., Garland-Kuntz,E.E., Sieng,M., Muralidharan,K., Van
            Camp,M.M., O'Neill,H., Mbongo,W., Selvia,A.F., Marti,A.T.,
            Everly,A., McKenzie,E. and Lyon,A.M.
  TITLE     Structure of phospholipase Cepsilon reveals an integrated RA1
            domain and previously unidentified regulatory elements
  JOURNAL   Commun Biol 3 (1), 445 (2020)
   PUBMED   32796910
  REMARK    GeneRIF: Structure of phospholipase Cepsilon reveals an integrated
            RA1 domain and previously unidentified regulatory elements.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8355)
  AUTHORS   Nash,C.A., Wei,W., Irannejad,R. and Smrcka,A.V.
  TITLE     Golgi localized beta1-adrenergic receptors stimulate Golgi PI4P
            hydrolysis by PLCepsilon to regulate cardiac hypertrophy
  JOURNAL   Elife 8, e48167 (2019)
   PUBMED   31433293
  REMARK    GeneRIF: The authors demonstrate that endogenous beta1-adrenergic
            receptors at the Golgi apparatus in cardiac myocytes are required
            to stimulate hypertrophic Epac/PLCe-dependent phosphatidylinositol
            4-phosphate hydrolysis at the Golgi, and that these intracellular
            beta1-adrenergic receptors can be accessed by physiological
            neurotransmitters, and synthetic b-blockers and agonists.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8355)
  AUTHORS   Li,W., Li,Y., Chu,Y., Wu,W., Yu,Q., Zhu,X. and Wang,Q.
  TITLE     PLCE1 promotes myocardial ischemia-reperfusion injury in H/R H9c2
            cells and I/R rats by promoting inflammation
  JOURNAL   Biosci Rep 39 (7) (2019)
   PUBMED   31217261
  REMARK    GeneRIF: PLCE1 promotes myocardial ischemia-reperfusion injury in
            H/R H9c2 cells and I/R rats by promoting inflammation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8355)
  AUTHORS   Nash,C.A., Brown,L.M., Malik,S., Cheng,X. and Smrcka,A.V.
  TITLE     Compartmentalized cyclic nucleotides have opposing effects on
            regulation of hypertrophic phospholipase Cepsilon signaling in
            cardiac myocytes
  JOURNAL   J Mol Cell Cardiol 121, 51-59 (2018)
   PUBMED   29885334
  REMARK    GeneRIF: These results reveal distinct pools of cyclic nucleotides
            that either inhibit PLCepsilon at the Golgi through PKA/PKG, or
            activate PLCepsilon at the Golgi through Epac. These data together
            reveal a new mechanism by which ANF and selective PDE inhibitors
            can protect against cardiac hypertrophy.
REFERENCE   6  (bases 1 to 8355)
  AUTHORS   Wing,M.R., Houston,D., Kelley,G.G., Der,C.J., Siderovski,D.P. and
            Harden,T.K.
  TITLE     Activation of phospholipase C-epsilon by heterotrimeric G protein
            betagamma-subunits
  JOURNAL   J Biol Chem 276 (51), 48257-48261 (2001)
   PUBMED   11641393
REFERENCE   7  (bases 1 to 8355)
  AUTHORS   Kelley,G.G., Reks,S.E., Ondrako,J.M. and Smrcka,A.V.
  TITLE     Phospholipase C(epsilon): a novel Ras effector
  JOURNAL   EMBO J 20 (4), 743-754 (2001)
   PUBMED   11179219
REFERENCE   8  (bases 1 to 8355)
  AUTHORS   Song,C., Hu,C.D., Masago,M., Kariyai,K., Yamawaki-Kataoka,Y.,
            Shibatohge,M., Wu,D., Satoh,T. and Kataoka,T.
  TITLE     Regulation of a novel human phospholipase C, PLCepsilon, through
            membrane targeting by Ras
  JOURNAL   J Biol Chem 276 (4), 2752-2757 (2001)
   PUBMED   11022048
REFERENCE   9  (bases 1 to 8355)
  AUTHORS   Lopez,I., Mak,E.C., Ding,J., Hamm,H.E. and Lomasney,J.W.
  TITLE     A novel bifunctional phospholipase c that is regulated by Galpha 12
            and stimulates the Ras/mitogen-activated protein kinase pathway
  JOURNAL   J Biol Chem 276 (4), 2758-2765 (2001)
   PUBMED   11022047
REFERENCE   10 (bases 1 to 8355)
  AUTHORS   Thomas,G.M., Geny,B. and Cockcroft,S.
  TITLE     Identification of a novel cytosolic poly-phosphoinositide-specific
            phospholipase C (PLC-86) as the major G-protein-regulated enzyme
  JOURNAL   EMBO J 10 (9), 2507-2512 (1991)
   PUBMED   1651229
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AF323615.1.
            
            [WARNING] On Oct 7, 2024 this sequence was replaced by NM_053758.3.
            
            On Aug 30, 2012 this sequence version replaced NM_053758.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF323615.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760384, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8355              AF323615.1         4-8358
FEATURES             Location/Qualifiers
     source          1..8355
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q53"
     gene            1..8355
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /note="phospholipase C, epsilon 1"
                     /db_xref="GeneID:114633"
                     /db_xref="RGD:69424"
     exon            1..225
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            226..1760
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     CDS             564..7409
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /EC_number="3.1.4.11"
                     /note="PLC-epsilon-1; phosphoinositide-specific
                     phospholipase C epsilon-1; phosphoinositide phospholipase
                     C-epsilon-1"
                     /codon_start=1
                     /product="1-phosphatidylinositol 4,5-bisphosphate
                     phosphodiesterase epsilon-1"
                     /protein_id="NP_446210.1"
                     /db_xref="GeneID:114633"
                     /db_xref="RGD:69424"
                     /translation="MTSEEMAASFLIPVPQRKVASAQSVAEERGEKVSEAGIPKTRAG
                     RQGGLTPRTISQRNEPEEESPRTDFSQVFSIARGELDSDENHNERCWEENVPGSTKNH
                     AVNCNSLLQSHQHALPPSQLCEVCDSVTEEHLCLQPGIPSPLERKVFPGIELEMEDSP
                     MDVSPLGNQPGIMESSGPHSDRNMAVFHFHYAGDRTMPGAFHTLSEKFILDDCANCVT
                     LPGGQQNKNYMAYTCKLVELTRTCGSKNGQLKCDHCTSLRDEYLCFESSCRKAEALSS
                     GGGFCEDGFTHGPSAKTFLNPLEEFSDNCEDVDDIFKGKKERSTLLVRRFCKNDREVK
                     KSVYTGTRAIVRTLPSGHIGLAAWSYVDQKKAGLMWPCGNGMRPLSTVDVRQSGRQRL
                     SEAQWCLIYSAVRREETEDTVGSLLHCSTQLPTPDTAHGRIGDGPCLKQCVRDSECEY
                     RATLQRTSIAQYITGSLLEATTSLGARSSLLSSFGGSTGRIMLKERQPGTSMANSSPV
                     PSSSAGISKELIDLQPLIQFPEEVASILTEQEQNIYRRVLPMDYLCFLTRDLSSPECQ
                     RSLPRLKACISESILMSQSGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSY
                     LVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSV
                     EYKKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLLKLCPRYSSQEEALEFVADY
                     SGQDNFLQRVGQNGLKNPEKELTVNSIFQIIRSCSRSLETEDEESASEGSGSRKNSLK
                     DKTRWQFIIGDLLDSDNDIFEKSKECDPHGSEESQKAFDHGTELIPWYVLSIQADVHQ
                     FLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWMKPPTASPAGARLKLGVLSNVAEP
                     GKFPSLGNAGVSGLVEGILDLFSVKAVYMGHPGIDIHTVCVQNKLSSMLLSETGVTLL
                     YGLQTTDNRLLHFVAPKHTAKMLFSGLLELTTAVRKIRKFPDQRQQWLRKQYVSFYQE
                     DGRYEGPTLAHAVELFGGRRWSTRNPSPGMSAKNAEKPNMQRNNTLGISTTKKKKKML
                     MRGESGEVTDDEMATRKAKMYRECRSRSGSDPQEANEQEDSEANVITNPPNPLHSRRA
                     YSLTTAGSPNLATGMSSPISAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFIEFVELFK
                     SFSIRSRKDLKDIFDIYSVPCNRSASESTPLYTNLTIEENTNDLQPDLDLLTRNVSDL
                     GLFMKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGE
                     HCTYDEILSIIQKFEPNISMCHQGLLSFEGFARFLMDKDNFASKNDESRENKKDLQLP
                     LSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSIELDCWDGDDGMPIIYHGHT
                     LTTKIPFKEVVEAIDRSAFITSDLPIIISIENHCSLPQQRKMAEIFKSVFGEKLVAKF
                     LFETDFSDDPMLPSPDQLRRKVLLKNKKLKAHQTPVDILKQKAHQLASMQTQAFTGGN
                     ANPPPASNEEEEDEEDEYDYDYESLSDDNILEDRPENKSCADKLQFEYNEEVPKRIKK
                     ADNSSGNKGKVYDMELGEEFYLPQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNSSG
                     SGRGKERKSRKSIFGNNPGRMSPGETASFNRTSGKSSCEGIRQIWEEPPLSPNTSLSA
                     IIRTPKCYHISSLNENAAKRLCRRYSQKLIQHTACQLLRTYPAATRIDSSNPNPLMFW
                     LHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKSCPMYQKFSPLERDL
                     DAMDPATYSLTIISGQNVCPSNSTGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWN
                     EQFLFRVHFEDLVFLRFAVVENNSSAITAQRIIPLKALKRGYRHLQLRNLHNEILEIS
                     SLFINSRRMEDNPSGSTRPASLMFNTEERKCSQTHKVTVHGVPGPEPFAVFTINEGTK
                     AKQLLQQILAVDQDTKLTAADYFLMEEKHFISKEKNECRKQPFQRAVGPEEDIVQILN
                     SWFPEEGYVGRIVLKPQQETLEEKNIVHDDREVILSSEEESFFVQVHDVSPEQPRTVI
                     KAPRVSTAQDVIQQTLCKAKYSYSILNNPNPCDYVLLEEVMKDAPNKKSSTPKSSQRI
                     LLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKRRGISFASELKKLTKSTKQTRGL
                     TSPPQLVASESVQSKEEKPMGALASGDTAGYQS"
     exon            1761..2046
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            2047..2363
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            2364..2509
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            2510..2768
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            2769..2974
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            2975..3650
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            3651..3833
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            3834..3951
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            3952..4060
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            4061..4183
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            4184..4320
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            4321..4559
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            4560..4621
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            4622..4787
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            4788..4895
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            4896..5012
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            5013..5171
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            5172..5301
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            5302..5423
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            5424..5541
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            5542..5673
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            5674..5958
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            5959..6122
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            6123..6384
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            6385..6503
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            6504..6632
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            6633..6842
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            6843..7001
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            7002..7220
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            7221..7443
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
     exon            7444..8338
                     /gene="Plce1"
                     /gene_synonym="Plce"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gagcgcggag cgcgggctgg cggcgggggc ggcgggctag agcagtggac ggcggctgcg
       61 cgccccgctg tgctctgccc ggcttctgtt cgctcctggg aatcggctgt gcggctgaca
      121 ctctgtaact gggactctcc acggctctgg ggacgcacgc gcgtggacca ggtccccagg
      181 gacgcggcac tctggtcccg ccgcacgtct ggctggagat ctaagggaac agaaaacact
      241 caaactggat ttttaaatca ttgcacatta acgatacaac cccaagttgc agaatgtgac
      301 cttgaacaaa atccttctaa aaaattagct tcaggcaaca gagaaaagaa aagtgaactg
      361 ttgttttgaa atactggtgt aaaacaaact gtccttgtag gaaattacaa ctggaaaatt
      421 ttgtttggct cttaatatat ctgaatgaaa ggaactgtca ccttacttct cacgactgga
      481 attatctgag attatctgtt gtctggacat gatcgccagg gaagggaaca cagaagagta
      541 gcaactcaag gacgctggtc aagatgacgt ccgaagaaat ggccgcttcc ttcctcatcc
      601 cggtgcctca aaggaaagtg gcttctgccc agtcagtggc agaggagcgg ggtgaaaagg
      661 tctcagaagc cggcattcct aagacacgag ctggcagaca gggtgggctc acccctcgta
      721 ccatctcaca gcggaacgaa cctgaggaag agtctcctag aactgatttt tcccaggtct
      781 tctcaatagc gaggggggaa ctagacagcg acgagaatca caatgaaagg tgctgggagg
      841 aaaatgtgcc aggttccacg aaaaaccacg cggttaactg caacagctta ttgcaaagcc
      901 accagcatgc gcttcccccg agccagctct gtgaagtctg tgactctgtc acagaagaac
      961 acctgtgttt gcagcctggg attccttctc cactggaaag gaaggtgttt cctgggatcg
     1021 aactggagat ggaggactct cccatggacg tgagcccttt gggaaaccag cctgggatca
     1081 tggagtccag cggacctcac tctgaccgca acatggcggt atttcatttc cattatgcag
     1141 gtgacaggac aatgccaggt gctttccata ccctgtcaga aaaattcatt ttggatgact
     1201 gtgcaaactg tgttactctt cctggggggc agcaaaacaa aaattacatg gcgtatactt
     1261 gcaaactggt ggagttgaca agaacctgtg gtagtaagaa tgggcagctg aagtgtgacc
     1321 actgcacctc gctgcgggat gaatacctgt gctttgaaag ctcctgccgg aaggctgagg
     1381 ctctctcctc aggtggtggc ttttgcgagg acggatttac tcatggtccc tctgccaaga
     1441 cttttctgaa ccctttggag gaattctctg ataattgtga agacgtggac gatattttta
     1501 aaggcaaaaa ggagcggtcc actttgttag tccgaaggtt ttgtaaaaat gacagggaag
     1561 ttaagaagtc tgtgtatact gggacaaggg ccattgtgag aaccctgcct tctggccaca
     1621 ttgggctggc tgcttggagt tacgtcgatc agaagaaagc tggtctcatg tggccttgtg
     1681 ggaatggaat gagacctctg tccacagtgg acgtaaggca aagtgggaga cagcgtctgt
     1741 ctgaagccca gtggtgtctg atctacagtg ccgtgagaag agaagagaca gaagatacgg
     1801 ttggctctct tctgcattgc tccacacagc tcccaacccc tgacacagcc catggaagga
     1861 tcggagatgg tccctgccta aagcaatgtg tgcgagacag cgagtgtgag taccgtgcca
     1921 ccctccaaag gacgtccata gctcagtaca tcaccggttc tctcctagag gcaaccacat
     1981 ctttaggagc aagaagcagc cttctcagtt cctttggagg atccactggg cgaataatgc
     2041 tgaaagaacg ccaaccgggc acttctatgg ccaattccag tcctgttcct tcaagttcag
     2101 ctggaatcag caaggagctg attgatctcc agcccctcat tcagttcccg gaggaagtag
     2161 ccagcatctt aacagaacag gaacagaaca tttaccgcag ggtcctgccc atggactacc
     2221 tctgtttctt aacccgggac ttgagttccc ccgagtgtca gagatcactg ccccgtctca
     2281 aagcatgcat ctcagaatca atcctcatgt ctcaaagtgg agagcacaat gcccttgaag
     2341 acctcgtgat gagatttaac gaggtgagct cctgggtaac atggctgatc ctcacggcag
     2401 gctccatgga ggagaagcgg gaagtctttt cttatttggt gcatgtggcc aaatgctgct
     2461 ggaacatggg caactacaat gctgtcatgg agttcctggc tggcctcagg tcaaggaaag
     2521 ttctgaagat gtggcagttc atggaccagt ctgacatcga gaccatgagg agcctgaaag
     2581 acgccatggc tcagcatgag tcttccgtgg agtacaagaa ggtagtgacc cgagccttgc
     2641 acatccccgg ctgcaaagtg gttcccttct gtggggtgtt cctgaaggag ctctgtgagg
     2701 tgcttgatgg tgcctctggc ctcctgaagc tctgcccacg atacagctcc caagaagaag
     2761 ccctggagtt tgtagcggat tacagtggac aagataactt cttacagcga gtggggcaga
     2821 atggcttgaa gaatccagag aaggagctca cggtcaacag catctttcag atcatccgga
     2881 gctgcagccg aagcttggag acggaggacg aggagagcgc cagcgaaggg agcggctcta
     2941 ggaaaaactc cctcaaggac aaaacccggt ggcagtttat aattggagat ttgctggatt
     3001 cagacaatga catctttgag aagtccaaag agtgcgaccc tcatggatca gaggagtcac
     3061 agaaagcctt tgaccatggg acagagctca tcccatggta tgtgctgtcc atccaagcgg
     3121 atgtgcacca gttcctcctg cagggggcca cagtcatcca ctatgaccag gacacacatc
     3181 tctccgcccg ctgctttctc cagttgcagc ctgacaatag taccttgacc tggatgaaac
     3241 ctcccactgc ctccccagct ggtgccagac tcaagctcgg tgtgcttagt aacgtggccg
     3301 aacctggcaa gttcccatca ctgggcaacg ctggggtgag cggcctggta gagggaatcc
     3361 tggatctgtt ttcagtgaag gctgtgtaca tgggacaccc tggcattgat atacacactg
     3421 tgtgtgtcca gaacaaactt agtagcatgc ttctgtcaga gactggggtg acgctgctct
     3481 atgggcttca gaccaccgac aatagattac tgcacttcgt ggcaccaaag cacacagcta
     3541 aaatgctttt cagtggattg ctggaactca ctacagctgt gagaaagata cggaagttcc
     3601 ctgaccaaag acaacagtgg cttcggaaac agtatgtcag cttctatcag gaggatggtc
     3661 ggtatgaagg cccaaccttg gcacatgctg tggagttgtt tggtggtagg agatggagca
     3721 ctagaaaccc cagccctggg atgtcagcaa agaatgccga gaagcccaac atgcagagaa
     3781 acaacaccct aggcataagc accaccaaga aaaagaagaa aatgctcatg aggggggaga
     3841 gtggagaggt gacggacgat gagatggcca cccgcaaggc caaaatgtac agagagtgtc
     3901 gcagccggag cggctctgat cctcaagagg ctaatgaaca ggaagactca gaggccaatg
     3961 tcatcactaa ccctcccaac cccctccatt ccagaagagc ctactcattg accacggctg
     4021 ggtcccccaa cttggctacc gggatgtcat ctcctatcag tgcctggagt agcagcagct
     4081 ggcatgggcg gatcaaagga ggaatgaaag gcttccagag cttcatggtc tcagacagta
     4141 acatgagttt cattgaattc gtcgagctgt tcaaatcatt cagcataagg agccgcaagg
     4201 acttgaagga catctttgac atctactctg taccctgtaa tcggtctgct tctgagtcaa
     4261 ccccactgta caccaacctg acaatcgaag aaaataccaa tgaccttcag cctgacctag
     4321 atttgctgac tagaaacgtc tccgacttgg ggctgttcat gaagagtaag cagcagcttt
     4381 ctgacaacca gaggcagata tctgacgcca ttgctgctgc aagcattgtg actaatggca
     4441 ctgggattga aagcacgtcc ctgggcatat ttggggtcgg catactccag ctcaacgact
     4501 tcctggtgaa ttgccaagga gaacactgca catatgatga aatcctcagc atcatccaga
     4561 agttcgagcc caacatcagc atgtgtcatc aaggtctatt gtccttcgaa gggtttgcaa
     4621 ggtttctgat ggataaagac aatttcgcct caaaaaatga cgagtcgcgg gagaacaaga
     4681 aagacctgca gctacccctc tcctactatt acatcgaatc ttcgcacaac acctacctca
     4741 ccggccatca gctcaagggg gagtcctctg tggagctcta cagccaggtc ctcttgcaag
     4801 gatgcagaag catagagctg gactgctggg atggagatga tgggatgccc attatttatc
     4861 atggacatac gctgacaacc aagatcccct tcaaggaagt ggtggaagcc atcgatcgca
     4921 gtgccttcat cacctccgac ctgccaatca tcatatccat tgagaaccat tgctcattgc
     4981 ctcagcaacg gaaaatggca gaaattttca agagtgtgtt tggagaaaag ctggtggcta
     5041 agttcttgtt cgagaccgat ttctcagatg acccaatgct tccctcacct gaccaactta
     5101 gaaggaaagt gcttcttaaa aacaagaagc taaaggccca tcagacacct gtcgacatcc
     5161 tcaaacaaaa ggctcatcag ttagcatcca tgcaaacaca ggctttcact ggtgggaatg
     5221 ccaacccgcc acctgccagt aatgaggaag aggaagacga ggaggacgaa tatgattatg
     5281 attacgagtc cctgtctgat gacaacattc tggaagacag acctgagaac aaatcatgtg
     5341 ccgacaagct tcagtttgag tacaatgaag aagtccccaa gaggataaag aaagcagaca
     5401 actcctccgg caataaggga aaggtctatg acatggaact gggagaggag ttttaccttc
     5461 ctcagaacaa aaaagaaagc aggcagattg caccggagct ctctgacctc gtcatctatt
     5521 gccaggcggt aaaatttcca ggcctgtcaa ctctaaattc atctggctct ggcagaggga
     5581 aagaaaggaa aagcaggaag tccatttttg gcaacaatcc cggcagaatg agcccagggg
     5641 agacagcgtc atttaacaga acatctggaa aaagttcttg tgaaggaatt cgacaaatct
     5701 gggaggaacc tccactcagc ccaaacacat ccctcagcgc catcattaga actcctaaat
     5761 gctatcatat ctcgtcactg aatgaaaatg ccgccaaacg tctgtgtcgc aggtattctc
     5821 agaaactgat ccagcacact gcttgccagc tgctgaggac ctacccggca gcgaccagaa
     5881 tcgactcatc caatcccaac cccctcatgt tctggctcca tgggatacag cttgtagctc
     5941 taaactacca gacggatgac cttcctttac atttaaacgc tgccatgttt gaggcgaatg
     6001 gtgggtgtgg ctatgttttg aagccccctg tcctgtggga taaaagctgt cccatgtatc
     6061 agaagttttc accactggaa agagacctgg acgccatgga tcctgctacc tattctttaa
     6121 ccatcatctc tggccagaat gtgtgtccta gtaacagcac cggaagtccg tgcattgaag
     6181 ttgacgtctt gggcatgccc ctggacagct gccatttccg cacaaaaccc atccaccgaa
     6241 acactctgaa ccccatgtgg aatgagcagt ttctcttccg ggttcacttt gaagatcttg
     6301 tatttcttcg ttttgcagtt gtggagaaca acagttcggc gataacggct cagagaatca
     6361 ttccactcaa agctttaaag agaggatatc ggcatcttca gctgcggaac ctccacaacg
     6421 agatcttgga aatctctagc ttattcataa acagcaggag gatggaagat aatccctctg
     6481 gcagtacccg gccagcctct ttgatgttca atactgaaga gaggaaatgt tctcagactc
     6541 acaaagtcac agtgcatggt gtcccaggtc cagagccctt tgctgttttc acaataaacg
     6601 aaggcaccaa ggcaaagcag cttctccaac agattttggc agttgaccag gacaccaaac
     6661 ttacagccgc agactatttt ctgatggaag agaagcattt tatatctaag gaaaagaatg
     6721 aatgcaggaa acaaccattc cagagagctg tgggcccaga ggaagatatt gtgcagattt
     6781 taaacagctg gttcccagaa gaaggctacg taggcaggat tgtcttaaag ccccaacaag
     6841 aaaccctaga agagaaaaac attgtccacg atgacagaga ggtgatcttg agctcagaag
     6901 aggagagttt ctttgtccaa gtgcacgatg tctctccaga gcagcctcga actgtcatca
     6961 aagccccacg tgtcagcact gcacaggatg tgattcagca gaccttgtgc aaagccaaat
     7021 attcctacag catcctgaac aaccccaatc cgtgtgacta tgtgcttttg gaagaggtaa
     7081 tgaaagatgc acccaataag aagtcttcta ccccgaagtc ctcacagagg atcctcttgg
     7141 accaagaatg tgtgtttcaa gcccaaagca agtggaaggg tgcagggaaa ttcatcctta
     7201 agctaaagga gcaggtgcag gcatccagag aagataaaag gaggggcatc tcctttgcaa
     7261 gtgaactcaa gaaactcacg aagtccacta agcaaacccg aggactcacc tcgcctcctc
     7321 agcttgtggc ctcagaaagt gtgcaaagca aggaggagaa acccatgggt gccttggcct
     7381 ctggtgacac agcgggctac cagtcgtgag ccagtgcagc acatttgatc cagggatatg
     7441 aaggggaagg cccttgatga gaagctatca agaatggaca tggtggaaag aagtatactt
     7501 tacctccatc acattcaaac tggaaattga tgatttccga actccagaac ctcctcacac
     7561 ccgatgagag gttcatgcag aggacaagca aaacggcaca gaactgccaa ccaacttcct
     7621 gaggaatgaa gcagggacac ccagggcatt gcccggggca ttatgggtgg aaaagccacc
     7681 cctcaagctg aaggcaacaa ggaacacctg taaagggcca ggcggtaaac attttagggt
     7741 cggggccacg tggcttctgt cgaattcagt tagctcagct actggatggc aaatgaagct
     7801 gtcgacaaca ggtacaagaa tgaacatctg actgaacaat aaaactttat ttatggacac
     7861 ttaaatcgga acctcatata attttcacat gtcacacatg tttaagtgtt tcaaccactt
     7921 aaaattgtaa aatctttcct agtttaaggg tcagacaaaa acaacatgag gctggctcga
     7981 ggaccagctt gctgacccag cttgggagaa ctgggagcca aaggcacaga ggttagaaaa
     8041 cctcgaaaat tggccgtggg tttaatgaga agtggaagtc tggggacatt ggaaatgtaa
     8101 atatgtcctg ttagatttgt tagcaattat tcaaattagt tcctatattg tgtacccagg
     8161 tgcactcttt gggctgcctt tcctcaaata gaaatgtttt tccgaaagct acgacccatg
     8221 cgtccacaga ctgtcccatt ttcagttctc acaaagtgaa tttagaatga tgtacaattt
     8281 agtgtctatc gagttgtgat gtcctcataa gaaaacctaa aagcaaaaag tagactgaaa
     8341 aaaaaaaaaa aaaaa
//
