LOCUS       NM_080688               2696 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Rattus norvegicus phospholipase C, delta 4 (Plcd4), mRNA.
ACCESSION   NM_080688
VERSION     NM_080688.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Maziarz,M., Broselid,S., DiGiacomo,V., Park,J.C., Luebbers,A.,
            Garcia-Navarrete,L., Blanco-Canosa,J.B., Baillie,G.S. and
            Garcia-Marcos,M.
  TITLE     A biochemical and genetic discovery pipeline identifies PLCdelta4b
            as a nonreceptor activator of heterotrimeric G-proteins
  JOURNAL   J Biol Chem 293 (44), 16964-16983 (2018)
   PUBMED   30194280
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Fukami,K., Nakao,K., Inoue,T., Kataoka,Y., Kurokawa,M.,
            Fissore,R.A., Nakamura,K., Katsuki,M., Mikoshiba,K., Yoshida,N. and
            Takenawa,T.
  TITLE     Requirement of phospholipase Cdelta4 for the zona pellucida-induced
            acrosome reaction
  JOURNAL   Science 292 (5518), 920-923 (2001)
   PUBMED   11340203
REFERENCE   3  (bases 1 to 2696)
  AUTHORS   Nagano,K., Fukami,K., Minagawa,T., Watanabe,Y., Ozaki,C. and
            Takenawa,T.
  TITLE     A novel phospholipase C delta4 (PLCdelta4) splice variant as a
            negative regulator of PLC
  JOURNAL   J Biol Chem 274 (5), 2872-2879 (1999)
   PUBMED   9915823
REFERENCE   4  (bases 1 to 2696)
  AUTHORS   Liu,N., Fukami,K., Yu,H. and Takenawa,T.
  TITLE     A new phospholipase C delta 4 is induced at S-phase of the cell
            cycle and appears in the nucleus
  JOURNAL   J Biol Chem 271 (1), 355-360 (1996)
   PUBMED   8550586
REFERENCE   5  (bases 1 to 2696)
  AUTHORS   Lee,S.B. and Rhee,S.G.
  TITLE     Molecular cloning, splice variants, expression, and purification of
            phospholipase C-delta 4
  JOURNAL   J Biol Chem 271 (1), 25-31 (1996)
   PUBMED   8550568
REFERENCE   6  (bases 1 to 2696)
  AUTHORS   Hempel,W.M. and DeFranco,A.L.
  TITLE     Expression of phospholipase C isozymes by murine B lymphocytes
  JOURNAL   J Immunol 146 (11), 3713-3720 (1991)
   PUBMED   2033248
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from U16655.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U16655.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132280, SAMD00132291
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="9"
                     /map="9q33"
     gene            1..2696
                     /gene="Plcd4"
                     /note="phospholipase C, delta 4"
                     /db_xref="GeneID:140693"
                     /db_xref="RGD:621025"
     exon            1..108
                     /gene="Plcd4"
                     /inference="alignment:Splign:2.1.0"
     exon            109..164
                     /gene="Plcd4"
                     /inference="alignment:Splign:2.1.0"
     CDS             143..2461
                     /gene="Plcd4"
                     /EC_number="3.1.4.11"
                     /note="PLC-delta-4; phosphoinositide phospholipase
                     C-delta-4"
                     /codon_start=1
                     /product="1-phosphatidylinositol 4,5-bisphosphate
                     phosphodiesterase delta-4"
                     /protein_id="NP_542419.1"
                     /db_xref="GeneID:140693"
                     /db_xref="RGD:621025"
                     /translation="MASQIQKLLTTNQDLLLMQKGTMMRKVRTKSWKKLRYFRLQDDG
                     MTVWHGRHLESISKPTFSISDVERIRKGQDSELLRYLVEEFPLEQGFTIVFNGRRPNL
                     DLVANSVEEAQTWMRGLQLLVDLVARMNYQEQLDQMLREWFQQADRNQDSRMSFREAQ
                     RLLLLMNVEMDEEYAFSLFQEADVSQSNTLDSEEFVQFYKALTKRTEIEELFENFSSD
                     KQKLTLLEFVDFLREEQKESDHSSDLALKLIDRYEPSENGRLLRVLSKDGFLSYLCSA
                     DGNIFNPDCLPIYQDMTQPLSHYYINSSHNTYLLGDQFCGQSSVEGYIRALKRGCRCV
                     EVDTWDGPDGEPVVYHGRTLTSRILFKDVLATLAQYAFQSSDYPLILSLDNHCTWEQQ
                     KTMAHHLIAILGEQLLSTTLEEQLIDIMPSPEQLRGKILVKGKKLRTIEVVESDKEEE
                     ELEKDEGSDLDPASAELDMQSQPESQEQASGNKSKNKKKFLLQSSTTILCPDLSALVV
                     YLRTAPFCSFTHSKENYHIYDISSFSESKAKNLIREAGNEFVQHNARQLCRVYPSGLR
                     TDSSNYNPQEHWNVGCQMVAMNMQTAGSAMDICDGLFRQNGGSGYVLKPEFLRDTQSS
                     FNPMKPVSLYKAQILVVQVISGQRLPKVDKTKETTVVDPLVRVELYGVPEDTKQQETS
                     YVENNGINPYWGETFYFQIQVPELAMLRFVVKDYSRTSRNNFIGQYTLPWTCMKHGYR
                     HVSLLSKDGTSLHPASIFVYTCMQEDLDMDEP"
     exon            165..323
                     /gene="Plcd4"
                     /inference="alignment:Splign:2.1.0"
     exon            324..552
                     /gene="Plcd4"
                     /inference="alignment:Splign:2.1.0"
     exon            553..682
                     /gene="Plcd4"
                     /inference="alignment:Splign:2.1.0"
     exon            683..914
                     /gene="Plcd4"
                     /inference="alignment:Splign:2.1.0"
     exon            915..1116
                     /gene="Plcd4"
                     /inference="alignment:Splign:2.1.0"
     exon            1117..1261
                     /gene="Plcd4"
                     /inference="alignment:Splign:2.1.0"
     exon            1262..1414
                     /gene="Plcd4"
                     /inference="alignment:Splign:2.1.0"
     exon            1415..1609
                     /gene="Plcd4"
                     /inference="alignment:Splign:2.1.0"
     exon            1610..1778
                     /gene="Plcd4"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..1895
                     /gene="Plcd4"
                     /inference="alignment:Splign:2.1.0"
     exon            1896..2068
                     /gene="Plcd4"
                     /inference="alignment:Splign:2.1.0"
     exon            2069..2204
                     /gene="Plcd4"
                     /inference="alignment:Splign:2.1.0"
     exon            2205..2354
                     /gene="Plcd4"
                     /inference="alignment:Splign:2.1.0"
     exon            2355..2462
                     /gene="Plcd4"
                     /inference="alignment:Splign:2.1.0"
     exon            2463..2695
                     /gene="Plcd4"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2696
                     /gene="Plcd4"
                     /note="17 A nucleotides"
ORIGIN      
        1 gaagcagtca agtgcaaagg agaaaaggag tcttctcatt ctcagtggag agcattgtgg
       61 agctcgggag acgacgctca cagacacagg attactatcc tacccacggt gactctggca
      121 ccagctggtg gagcagtggg ggatggcatc tcagattcaa aagctactga ccactaatca
      181 ggacctgctg ctgatgcaaa aaggaacgat gatgcgcaag gtgaggacca aaagctggaa
      241 gaagctaaga tacttcagac ttcaggatga cggcatgacg gtctggcatg ggcggcacct
      301 tgaaagcata tccaagccca ctttttcaat ctctgatgtg gagaggatcc gtaagggcca
      361 ggattctgag ctgttgcgct atctggtgga agagttcccc ctggagcaag gcttcaccat
      421 tgtctttaat gggcgccgcc ccaacctgga cctagtggcc aacagtgttg aagaggccca
      481 gacttggatg cgaggactcc agctgttggt ggatctcgtc gccagaatga actaccagga
      541 gcagctggat caaatgttga gagaatggtt ccaacaagca gacagaaacc aggacagccg
      601 gatgagtttc agagaagctc aacggctgct gcttctgatg aatgtggaaa tggatgaaga
      661 atatgccttc agtctttttc aggaagcaga tgtttcccaa tctaatactc tggattcaga
      721 agaatttgta cagttctata aggccctgac taagcgcact gagattgaag aactatttga
      781 gaacttttca tctgacaaac agaagctgac cttgctggaa tttgtagatt tccttagaga
      841 ggaacagaaa gaaagcgacc attcctctga ccttgctctg aaactcattg accgctatga
      901 gccttctgag aatggcagat tgctacgtgt gctgagcaag gatggcttcc tcagctacct
      961 ctgctcagcg gatggaaaca tcttcaaccc cgactgcctc cctatctatc aggatatgac
     1021 tcagcctctg agtcactact acatcaactc ctctcacaac acctacctac tgggtgacca
     1081 gttttgtggc cagagcagcg ttgaaggata catacgggcc ttgaaacggg ggtgccgctg
     1141 tgtggaggtg gatacatggg acggacctga cggagagccc gttgtttatc atgggcgtac
     1201 actgacctct cgtatcctgt tcaaagatgt tttggcaaca ttagcacaat atgccttcca
     1261 gtcgtcagac tacccactca tcttatctct ggataaccac tgcacttggg aacagcagaa
     1321 gaccatggca caccatctga ttgcgatcct gggagagcag ctgctgagta ccaccctaga
     1381 ggaacaactg attgatataa tgccctcgcc agagcagctt cgggggaaga tcttggtaaa
     1441 aggaaagaag ttgagaacaa ttgaggttgt tgagtctgac aaggaggaag aagagctgga
     1501 gaaagatgag gggtcagatc tggacccagc ctccgctgag cttgacatgc agtctcagcc
     1561 cgagtcccag gagcaggcct ctgggaacaa gagcaagaac aagaagaagt ttcttctcca
     1621 gagttccaca accatcctat gcccagacct atctgccctg gttgtctact tgaggactgc
     1681 tcccttctgc agcttcactc actccaaaga aaactaccac atctacgaca tttcatcctt
     1741 ttctgagtcc aaggccaaga acctcatcag ggaggcaggc aatgagtttg tacagcacaa
     1801 tgccagacag ctatgccgtg tataccccag tggtctgagg acagattcat cgaactataa
     1861 cccccaggaa cactggaatg ttggctgcca aatggtggcc atgaacatgc agactgcagg
     1921 aagtgcaatg gacatctgtg atgggctctt ccgccagaat gggggctctg gctatgtgct
     1981 gaagccagaa ttcctgagag atacccagag ttccttcaat cccatgaagc ccgttagtct
     2041 ttataaagcc cagatccttg tagtccaggt gatcagtggt caaagactcc ccaaagtgga
     2101 caaaaccaaa gagacaaccg ttgtggatcc actggtgaga gtagagctct atggtgttcc
     2161 tgaagacaca aagcagcaag agaccagcta tgtggagaac aatggtatta atccatactg
     2221 gggggagaca ttctatttcc agatccaggt gcctgaactt gctatgctgc gttttgtggt
     2281 aaaggattat agcaggacat cccgaaataa ctttataggc caatacaccc tgccttggac
     2341 ctgcatgaaa catggctacc gacatgtatc cttgctctcc aaagacggca ctagcctcca
     2401 tccagcctcc atctttgtgt acacctgcat gcaagaagac ctggatatgg atgagccctg
     2461 agaaacacag ggagggtctt gaagcaggac aatctacaga tgcactcttt tccaacctac
     2521 aacttcctca gcatccagag ccagccaaag gatcagtggc ggaccaaggt tcccaggtga
     2581 aggttgagga agaatcctga cccagaacta tgactctaga gaacagtgca cagccgagtc
     2641 cccactggac tgctgctcct tagctggtct aaatagagct tctctgtcca acttga
//
