LOCUS       NM_024353               5297 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus phospholipase C, beta 4 (Plcb4), mRNA.
ACCESSION   NM_024353 XM_346671
VERSION     NM_024353.2
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Sekerkova,G., Watanabe,M., Martina,M. and Mugnaini,E.
  TITLE     Differential distribution of phospholipase C beta isoforms and
            diaglycerol kinase-beta in rodents cerebella corroborates the
            division of unipolar brush cells into two major subtypes
  JOURNAL   Brain Struct Funct 219 (2), 719-749 (2014)
   PUBMED   23503970
  REMARK    GeneRIF: This study demonistrated that Plcb4 distribuate in rat
            cerebellum.
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   Otaegui,D., Querejeta,R., Arrieta,A., Lazkano,A., Bidaurrazaga,A.,
            Arriandiaga,J.R., Aldazabal,P. and Garro,M.A.
  TITLE     Phospholipase Cbeta4 isozyme is expressed in human, rat, and murine
            heart left ventricles and in HL-1 cardiomyocytes
  JOURNAL   Mol Cell Biochem 337 (1-2), 167-173 (2010)
   PUBMED   19856080
  REMARK    GeneRIF: Presence of PLCbeta(4) in the heart and in HL-1
            cardiomyocytes showing a different species-dependent pattern of
            expression of the PLCbeta((1-4)) transcripts.
REFERENCE   3  (bases 1 to 5297)
  AUTHORS   Hozumi,Y., Watanabe,M. and Goto,K.
  TITLE     Signaling cascade of diacylglycerol kinase beta in the pituitary
            intermediate lobe: dopamine D2 receptor/phospholipase
            Cbeta4/diacylglycerol kinase beta/protein kinase Calpha
  JOURNAL   J Histochem Cytochem 58 (2), 119-129 (2010)
   PUBMED   19826069
  REMARK    GeneRIF: functional roles of DGK in the pituitary intermediate lobe
REFERENCE   4  (bases 1 to 5297)
  AUTHORS   Drin,G. and Scarlata,S.
  TITLE     Stimulation of phospholipase Cbeta by membrane interactions,
            interdomain movement, and G protein binding--how many ways can you
            activate an enzyme?
  JOURNAL   Cell Signal 19 (7), 1383-1392 (2007)
   PUBMED   17524618
  REMARK    Review article
REFERENCE   5  (bases 1 to 5297)
  AUTHORS   Toyono,T., Kataoka,S., Seta,Y. and Toyoshima,K.
  TITLE     Expression of phospholipase C-beta4 in rat circumvallate taste buds
  JOURNAL   Chem Senses 30 Suppl 1, i27-i28 (2005)
   PUBMED   15738151
  REMARK    GeneRIF: analysis of phospholipase C-beta4 expression in rat
            circumvallate taste buds
REFERENCE   6  (bases 1 to 5297)
  AUTHORS   Haley,J.E., Abogadie,F.C., Fernandez-Fernandez,J.M., Dayrell,M.,
            Vallis,Y., Buckley,N.J. and Brown,D.A.
  TITLE     Bradykinin, but not muscarinic, inhibition of M-current in rat
            sympathetic ganglion neurons involves phospholipase C-beta 4
  JOURNAL   J Neurosci 20 (21), RC105 (2000)
   PUBMED   11050147
REFERENCE   7  (bases 1 to 5297)
  AUTHORS   Adamski,F.M., Timms,K.M. and Shieh,B.H.
  TITLE     A unique isoform of phospholipase Cbeta4 highly expressed in the
            cerebellum and eye
  JOURNAL   Biochim Biophys Acta 1444 (1), 55-60 (1999)
   PUBMED   9931434
REFERENCE   8  (bases 1 to 5297)
  AUTHORS   Kim,M.J., Min,D.S., Ryu,S.H. and Suh,P.G.
  TITLE     A cytosolic, galphaq- and betagamma-insensitive splice variant of
            phospholipase C-beta4
  JOURNAL   J Biol Chem 273 (6), 3618-3624 (1998)
   PUBMED   9452490
REFERENCE   9  (bases 1 to 5297)
  AUTHORS   Lee,C.W., Park,D.J., Lee,K.H., Kim,C.G. and Rhee,S.G.
  TITLE     Purification, molecular cloning, and sequencing of phospholipase
            C-beta 4
  JOURNAL   J Biol Chem 268 (28), 21318-21327 (1993)
   PUBMED   8407970
REFERENCE   10 (bases 1 to 5297)
  AUTHORS   Kim,M.J., Bahk,Y.Y., Min,D.S., Lee,S.J., Ryu,S.H. and Suh,P.G.
  TITLE     Cloning of cDNA encoding rat phospholipase C-beta 4, a new member
            of the phospholipase C
  JOURNAL   Biochem Biophys Res Commun 194 (2), 706-712 (1993)
   PUBMED   7688223
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            [WARNING] On Jul 16, 2024 this sequence was replaced by
            NM_024353.3.
            
            On Nov 26, 2020 this sequence version replaced NM_024353.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: L15556.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760433, SAMN11044071
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-118               JAXUCZ010000003.1  143405973-143406090
            119-187             JAXUCZ010000003.1  143493425-143493493
            188-245             JAXUCZ010000003.1  143559809-143559866
            246-344             JAXUCZ010000003.1  143631521-143631619
            345-425             JAXUCZ010000003.1  143666503-143666583
            426-485             JAXUCZ010000003.1  143667405-143667464
            486-629             JAXUCZ010000003.1  143669087-143669230
            630-709             JAXUCZ010000003.1  143691090-143691169
            710-763             JAXUCZ010000003.1  143693342-143693395
            764-845             JAXUCZ010000003.1  143698982-143699063
            846-946             JAXUCZ010000003.1  143699917-143700017
            947-1004            JAXUCZ010000003.1  143700656-143700713
            1005-1113           JAXUCZ010000003.1  143707598-143707706
            1114-1324           JAXUCZ010000003.1  143710706-143710916
            1325-1418           JAXUCZ010000003.1  143712099-143712192
            1419-1498           JAXUCZ010000003.1  143714310-143714389
            1499-1583           JAXUCZ010000003.1  143714883-143714967
            1584-1674           JAXUCZ010000003.1  143717634-143717724
            1675-1770           JAXUCZ010000003.1  143718231-143718326
            1771-1874           JAXUCZ010000003.1  143722622-143722725
            1875-2016           JAXUCZ010000003.1  143727881-143728022
            2017-2101           JAXUCZ010000003.1  143728995-143729079
            2102-2226           JAXUCZ010000003.1  143729435-143729559
            2227-2278           JAXUCZ010000003.1  143731347-143731398
            2279-2381           JAXUCZ010000003.1  143732735-143732837
            2382-2546           JAXUCZ010000003.1  143733907-143734071
            2547-2751           JAXUCZ010000003.1  143736389-143736593
            2752-2840           JAXUCZ010000003.1  143741417-143741505
            2841-2991           JAXUCZ010000003.1  143742341-143742491
            2992-3041           JAXUCZ010000003.1  143745148-143745197
            3042-3107           JAXUCZ010000003.1  143745446-143745511
            3108-3223           JAXUCZ010000003.1  143753317-143753432
            3224-3299           JAXUCZ010000003.1  143758037-143758112
            3300-3475           JAXUCZ010000003.1  143760552-143760727
            3476-3577           JAXUCZ010000003.1  143763048-143763149
            3578-3635           JAXUCZ010000003.1  143768089-143768146
            3636-3722           JAXUCZ010000003.1  143768570-143768656
            3723-5297           JAXUCZ010000003.1  143773550-143775124
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q36"
     gene            1..5297
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /note="phospholipase C, beta 4"
                     /db_xref="GeneID:25031"
                     /db_xref="RGD:3345"
     exon            1..118
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            119..187
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            188..245
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            246..344
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     CDS             261..3791
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /EC_number="3.1.4.11"
                     /note="PLC-beta-4; phosphoinositide phospholipase C;
                     phosphoinositide phospholipase C-beta-4"
                     /codon_start=1
                     /product="1-phosphatidylinositol 4,5-bisphosphate
                     phosphodiesterase beta-4"
                     /protein_id="NP_077329.2"
                     /db_xref="GeneID:25031"
                     /db_xref="RGD:3345"
                     /translation="MAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEF
                     GFFLTWKSEGKEGQVLECSLINSIRLAAIPKDPKILAALESVGKSENDLEGRILCVCS
                     GTDLVNIGFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFLTNT
                     SGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICP
                     RTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYE
                     PDEELKKKGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTG
                     RQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAI
                     KETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPS
                     PNDLKRKILIKNKRLKPEVEKKQLEALKSMMEAGESAAPASILEDDNEEEIESAADQE
                     EEAHPEYKFGNELSADDFSHKEAVANSVKKGLVTVEDEQAWMASYKYVGATTNIHPYL
                     STMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIY
                     PKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMR
                     RPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFR
                     TRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYR
                     HISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAM
                     GIETSDIADVPSDTSKNDKKGKANPAKANVTPQSSSELRPTTTAALGSGQEAKKGIEL
                     IPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKE
                     KSTHEKILEKAMKKKGGSNCLEIKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSEMIN
                     THSAEEQEIRDLHLSQQCELLRKLLINAHEQQTQQLKLSHDRESKEMRAHQAKISMEN
                     SKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLE
                     FLEKQNEQAKEMQQMVKLEAEMDRRPATVV"
     exon            345..425
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            426..485
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            486..629
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            630..709
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            710..763
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            764..845
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            846..946
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            947..1004
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1113
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            1114..1324
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            1325..1418
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            1419..1498
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            1499..1583
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            1584..1674
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            1675..1770
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            1771..1874
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            1875..2016
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            2017..2101
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            2102..2226
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            2227..2278
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            2279..2381
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            2382..2546
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            2547..2751
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            2752..2840
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            2841..2991
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            2992..3041
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            3042..3107
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            3108..3223
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            3224..3299
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            3300..3475
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            3476..3577
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            3578..3635
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            3636..3722
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
     exon            3723..5297
                     /gene="Plcb4"
                     /gene_synonym="Beta4aa; RATBETA4AA"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gccgcgccgc aaacaggctc tcacaggcgc gcacacagac acgcgcgcgc gcacacagcg
       61 gggttcccgg aggcggcggt ggccgcatcc ccagcagcga ctcgcctcga agtgacagga
      121 gcctgctggc tagtttctct gtgagtgctg aaaaccattt gagaggattc tcaacaaagc
      181 accctggggt ctgtgcacag gattcgatca gttagacctc ccttgctgct gcacctaacc
      241 atctggtctt gaacaccatc atggccaaac cttacgaatt taactggcag aaggaggtac
      301 cctctttctt gcaagaagga gcagtttttg acagatatga agaggaatct ttcgtgtttg
      361 agcccaactg cctcttcaaa gtggatgaat tcggcttctt cctgacctgg aagagtgaag
      421 gcaaggaagg acaagtgcta gaatgttccc tcatcaacag cattcggctg gcagccatac
      481 caaaggatcc caaaatcctg gctgctctcg agtctgttgg aaaatctgaa aatgatctgg
      541 aagggcggat attgtgtgtc tgcagcggca cggatctggt gaatattggc ttcacttaca
      601 tggtggctga aaacccagaa ataactaagc aatgggtaga aggcctgaga tcaatcattc
      661 acaacttcag ggcaaacaac gtcagtccga tgacatgcct caagaaacac tggatgaaac
      721 tggcctttct gaccaacaca agtggcaaaa tcccagtgag gagtattact agaaccttcg
      781 catcagggaa aacagaaaag gtgatctttc aagcactcaa ggaattaggt ctccccagtg
      841 gaaagaatga tgaaattgaa cctgctgcat ttacctatga aaagttctat gaactgacac
      901 agaagatttg tcctcggaca gatatagaag atctttttaa aaaaatcaat ggagacaaaa
      961 ctgattattt aacggtagac caattagtga gctttctaaa cgaacatcag cgagatcctc
     1021 ggctgaatga aattttattc ccgttttatg atgctaaaag agcaatgcag atcattgaga
     1081 tgtatgaacc tgatgaagag ttgaagaaaa aaggcctcat atccagtgat ggattctgca
     1141 gatatctcat gtcagatgaa aatgcccccg tcttcctaga tcgcttagaa ctttaccagg
     1201 agatggacca ccccctggcc cattacttca tcagttcctc tcacaacacc tatctcaccg
     1261 gccggcagtt tggcggaaag tcttcagtgg aaatgtaccg acaagtcctc ctggctggtt
     1321 gcaggtgtgt ggaacttgac tgttgggatg gaaaaggtga agatcaggaa ccaataataa
     1381 ctcacggaaa agcaatgtgt acagacatcc tttttaagga tgtaatccag gccatcaagg
     1441 aaacagcgtt tgtcacatca gaatatcctg taattctctc ctttgaaaac cactgcagca
     1501 aatatcaaca gtacaagatg tccaagtatt gtgaagatct atttggggat ctcctgttga
     1561 aacaagcact tgagtcacat ccacttgaac cagggaggcc cttgccatct cctaatgacc
     1621 tcaaaagaaa aatactcatc aaaaataaaa ggctgaagcc tgaagttgaa aaaaaacagc
     1681 tggaggcttt gaaaagcatg atggaggctg gagaatcagc tgccccggcc agcatcttgg
     1741 aagatgacaa tgaggaggaa atagaaagcg cagctgatca agaggaagaa gcccaccctg
     1801 aatacaagtt tggaaatgaa ctttctgccg atgacttcag tcacaaggaa gcggttgcaa
     1861 acagcgtcaa gaagggcctg gtcaccgtag aagatgagca ggcatggatg gcatcttata
     1921 aatacgtagg tgctaccacg aatatccatc cgtacttgtc cacgatgatc aactatgcac
     1981 agcctgtgaa gtttcaaggt ttccatgtgg ctgaagagcg caacattcat tataacatgt
     2041 cttcttttaa cgagtcggtt ggccttggct acttgaagac gcacgcgatt gagtttgtaa
     2101 attacaataa gcgacaaatg agccgcattt accccaaggg aggccgagtt gattccagta
     2161 attacatgcc tcagattttc tggaacgctg gttgccagat ggtttcactg aactatcaaa
     2221 ccccagattt agcgatgcaa ttgaatcaag gaaaatttga gtataacgga tcctgcggat
     2281 acctcctcaa gccagacttc atgagacggc ctgatcggac atttgatccc ttctctgaaa
     2341 cccctgtgga cggggttatt gcagccacat gctcagtgca ggttatatca gggcagttcc
     2401 tgtcagataa gaagatcggc acatacgtgg aagtcgatat gtacgggctg cccaccgaca
     2461 ccatacggaa ggagttccga acccgcatgg ttatgaacaa tggactcaac ccagtttaca
     2521 atgaagaatc gttcgtgttt cggaaggtga tcctgcctga cctggctgtc ctgagaatcg
     2581 ctgtgtacga cgacaacaac aagctcattg gccagaggat ccttcctctg gatggcctcc
     2641 aggcaggcta ccgacacatc tccctgagaa atgagggaaa caaaccgtta tcactgccaa
     2701 caattttctg caatatcgtt cttaaaacat atgtgcctga tggatttgga gatattgtgg
     2761 atgctctatc cgatccaaag aaattccttt caatcacaga gaagagagca gaccaaatga
     2821 gagcaatggg cattgaaact agtgacatag cagatgtgcc cagtgacact tccaaaaatg
     2881 acaagaaagg caaggccaac ccagccaaag cgaatgtaac ccctcagagc agctctgagc
     2941 tcagaccaac caccacagct gccctgggct ctggccagga agccaagaaa ggtattgaac
     3001 ttatccctca agtgaggata gaagatttaa agcaaatgaa ggcttacttg aagcatttaa
     3061 agaaacaaca aaaggagtta aactctttaa agaagaaaca cgcaaaggag cacagtacca
     3121 tgcagaagtt acactgcaca caagtggaca aaatcgtggc ccagtatgac aaggagaagt
     3181 cgactcatga gaaaatccta gagaaggcca tgaagaagaa ggggggaagt aattgccttg
     3241 aaataaaaaa agaaacagaa attaagattc agaccctgac atcggatcac aaatctaagg
     3301 tcaaggagat tgtggcgcag cacacaaagg agtggtcaga aatgatcaac acgcacagcg
     3361 ccgaggagca ggaaatccgg gacctgcacc tgagccagca gtgtgagctg ctgaggaagc
     3421 tgctcatcaa cgctcatgag cagcagaccc agcagctgaa gctctcccat gacagggaaa
     3481 gcaaagagat gcgagcccat caggctaaga tttctatgga aaatagcaaa gccatcagtc
     3541 aagataaatc tatcaagaat aaggcagaac gggagaggcg agtcagggag ttgaacagca
     3601 gcaacactaa gaagtttcta gaagaaagaa agagactggc aatgaagcag tcgaaagaaa
     3661 tggatcagtt gaaaaaggtc cagctggagc acctagaatt cctagagaaa cagaatgagc
     3721 aggcgaagga gatgcagcag atggtgaaat tggaagccga gatggaccgc agaccagcaa
     3781 cagtagtatg aaactccaga atgcaaactg aagctggaag accatagaac accagaagat
     3841 cacgcccagc ttcagaggac agactccatg gacaaaagct gttttctttt gtgtttcttt
     3901 tatgtgtaga cacggtgtaa cctgaaacca catggactgg ggatgaccaa atgttcttct
     3961 acttaacaat ttgactattg aatttctttg gccaaccaaa agtagctatg tacacacaca
     4021 cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca gaaatcccct
     4081 attcctgaca ggcagagttg aaccataatc cacaacttaa acatgttggc taggggacag
     4141 catcacaagc cagtgggctt ggtgataaca actctgcttt gtggtgcatt aggacatgtt
     4201 tgagctgctg ctgcaaaaac aaattagtgc attagtaact taatagcaag cgcatgcact
     4261 agaagaaaca actccgtcta cagttcatta gcagaagtgg tggtctgcta tacaaatcac
     4321 tttgcagaac tttctacacc taagttacct catgagtaag tgccatgtct ttaccatgcc
     4381 ataagatgct aacttcctgt aaatgtggca gaatttgtta ggacaataga gacacacaaa
     4441 acggtgtacc tgtgccaagc tcatcagtgc gccaaggggg actcagcagg ctcgtttcgg
     4501 acagtttaca gctgactctg ctttgtagga agtgcttctg cagatgcctc atgctgcaat
     4561 tcactacaca ctatcaggag ggtggagtgg ctattggcaa atgtttgtaa atgtgaccat
     4621 gtacaaagta tttatactcc ttaattctca tggttatagc gcaaagtcat ctaaacatta
     4681 ccacacgaca agactagtaa acaggaatca tagatgtttt gcatgatgca caagcaccaa
     4741 ttatgactaa cccattgaga cacagttaac ctaacaccaa ataaataccg gttaagtcaa
     4801 tgtatggcac agaatattat ttgactatca agacttttag cataatgaaa aaagtctata
     4861 tatatgtgta tatgaattat gtgggcattc ttgatacttc tagtttgaaa aaatacatta
     4921 atttaatttt acacaaaaat atttatgcag attttcagac tttcatatca ggaaataacc
     4981 tttttatgtc tgttaaatat ccaaacgatt tgctaccgtg ttcatctgca tggtcttctc
     5041 acctgctgta gttgccttgc aggtggtgca taaagtggac gggtgggatg agtcctgttg
     5101 ccccaaccca gaggagcgtg cggagacccg gtagcctagg agtcagattc ctgattttag
     5161 gatgcaaaac gccaacttaa ttgttgtata tggttgtaca aatagattct gtgtgttcct
     5221 ctgaggaggg cagagaagag tatgatacca tgaaggctga aattcaacgt acaacttctc
     5281 taataaaaaa aaaaaaa
//
