LOCUS       NM_001077641            5485 bp    mRNA    linear   ROD 09-APR-2024
DEFINITION  Rattus norvegicus phospholipase C beta 1 (Plcb1), mRNA.
ACCESSION   NM_001077641 XM_017591466 XM_342524
VERSION     NM_001077641.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Park,J., Kim,S.H., Kim,Y.J., Kim,H., Oh,Y., Choi,K.Y., Kim,B.C.,
            Lee,K.H. and Song,W.K.
  TITLE     Elevation of phospholipase C-beta1 expression by amyloid-beta
            facilitates calcium overload in neuronal cells
  JOURNAL   Brain Res 1788, 147924 (2022)
   PUBMED   35469845
  REMARK    GeneRIF: Elevation of phospholipase C-beta1 expression by
            amyloid-beta facilitates calcium overload in neuronal cells.
REFERENCE   2  (bases 1 to 5485)
  AUTHORS   Lee,J. and Kwag,J.
  TITLE     Activation of PLCbeta1 enhances endocannabinoid mobilization to
            restore hippocampal spike-timing-dependent potentiation and
            contextual fear memory impaired by Alzheimer's amyloidosis
  JOURNAL   Alzheimers Res Ther 13 (1), 165 (2021)
   PUBMED   34625112
  REMARK    GeneRIF: Activation of PLCbeta1 enhances endocannabinoid
            mobilization to restore hippocampal spike-timing-dependent
            potentiation and contextual fear memory impaired by Alzheimer's
            amyloidosis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5485)
  AUTHORS   Garwain,O., Yerramilli,V.S., Romero,K. and Scarlata,S.
  TITLE     The Galphaq/phospholipase Cbeta signaling system represses tau
            aggregation
  JOURNAL   Cell Signal 71, 109620 (2020)
   PUBMED   32247043
  REMARK    GeneRIF: The Galphaq/phospholipase Cbeta signaling system represses
            tau aggregation.
REFERENCE   4  (bases 1 to 5485)
  AUTHORS   Garwain,O., Valla,K. and Scarlata,S.
  TITLE     Phospholipase Cbeta1 regulates proliferation of neuronal cells
  JOURNAL   FASEB J 32 (5), 2891-2898 (2018)
   PUBMED   29401590
  REMARK    GeneRIF: results present a model in which the transient high
            expression of PLCbeta1 that occurs at the onset of differentiation
            arrests cells in the G1 phase through its association with CDK16
            and allows CDK16 to transition to its postmitotic function of
            neurite outgrowth and trafficking of synaptic vesicles.
REFERENCE   5  (bases 1 to 5485)
  AUTHORS   Garwain,O. and Scarlata,S.
  TITLE     Phospholipase Cbeta-TRAX Association Is Required for PC12 Cell
            Differentiation
  JOURNAL   J Biol Chem 291 (44), 22970-22976 (2016)
   PUBMED   27624933
  REMARK    GeneRIF: PLCbeta through its ability to bind TRAX plays a key role
            in switching PC12 cells to their differentiated state.
REFERENCE   6  (bases 1 to 5485)
  AUTHORS   Bahk,Y.Y., Lee,Y.H., Lee,T.G., Seo,J., Ryu,S.H. and Suh,P.G.
  TITLE     Two forms of phospholipase C-beta 1 generated by alternative
            splicing
  JOURNAL   J Biol Chem 269 (11), 8240-8245 (1994)
   PUBMED   7510682
REFERENCE   7  (bases 1 to 5485)
  AUTHORS   Smrcka,A.V. and Sternweis,P.C.
  TITLE     Regulation of purified subtypes of phosphatidylinositol-specific
            phospholipase C beta by G protein alpha and beta gamma subunits
  JOURNAL   J Biol Chem 268 (13), 9667-9674 (1993)
   PUBMED   8387502
REFERENCE   8  (bases 1 to 5485)
  AUTHORS   Mazzoni,M., Bertagnolo,V., Neri,L.M., Carini,C., Marchisio,M.,
            Milani,D., Manzoli,F.A. and Capitani,S.
  TITLE     Discrete subcellular localization of phosphoinositidase C beta,
            gamma and delta in PC12 rat pheochromocytoma cells
  JOURNAL   Biochem Biophys Res Commun 187 (1), 114-120 (1992)
   PUBMED   1325785
REFERENCE   9  (bases 1 to 5485)
  AUTHORS   Berstein,G., Blank,J.L., Jhon,D.Y., Exton,J.H., Rhee,S.G. and
            Ross,E.M.
  TITLE     Phospholipase C-beta 1 is a GTPase-activating protein for Gq/11,
            its physiologic regulator
  JOURNAL   Cell 70 (3), 411-418 (1992)
   PUBMED   1322796
REFERENCE   10 (bases 1 to 5485)
  AUTHORS   Suh,P.G., Ryu,S.H., Moon,K.H., Suh,H.W. and Rhee,S.G.
  TITLE     Cloning and sequence of multiple forms of phospholipase C
  JOURNAL   Cell 54 (2), 161-169 (1988)
   PUBMED   3390863
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            On or before Jun 4, 2021 this sequence version replaced
            XM_017591466.2, NM_001077641.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643298.18853.1,
                                           SRR26643297.13105.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760393
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-566               JAXUCZ010000003.1  142512765-142513330
            567-644             JAXUCZ010000003.1  142539777-142539854
            645-713             JAXUCZ010000003.1  142716859-142716927
            714-851             JAXUCZ010000003.1  142944346-142944483
            852-931             JAXUCZ010000003.1  142979645-142979724
            932-985             JAXUCZ010000003.1  142981388-142981441
            986-1061            JAXUCZ010000003.1  142982436-142982511
            1062-1162           JAXUCZ010000003.1  142993305-142993405
            1163-1329           JAXUCZ010000003.1  142994871-142995037
            1330-1476           JAXUCZ010000003.1  143031343-143031489
            1477-1634           JAXUCZ010000003.1  143046481-143046638
            1635-1717           JAXUCZ010000003.1  143061197-143061279
            1718-1802           JAXUCZ010000003.1  143069596-143069680
            1803-1980           JAXUCZ010000003.1  143072830-143073007
            1981-2048           JAXUCZ010000003.1  143077691-143077758
            2049-2145           JAXUCZ010000003.1  143080480-143080576
            2146-2230           JAXUCZ010000003.1  143082509-143082593
            2231-2355           JAXUCZ010000003.1  143084613-143084737
            2356-2510           JAXUCZ010000003.1  143087198-143087352
            2511-2675           JAXUCZ010000003.1  143090391-143090555
            2676-2775           JAXUCZ010000003.1  143091242-143091341
            2776-2880           JAXUCZ010000003.1  143092518-143092622
            2881-2990           JAXUCZ010000003.1  143093610-143093719
            2991-3123           JAXUCZ010000003.1  143103781-143103913
            3124-3177           JAXUCZ010000003.1  143106354-143106407
            3178-3397           JAXUCZ010000003.1  143108711-143108930
            3398-3578           JAXUCZ010000003.1  143114662-143114842
            3579-3655           JAXUCZ010000003.1  143137010-143137086
            3656-3745           JAXUCZ010000003.1  143137188-143137277
            3746-3803           JAXUCZ010000003.1  143138138-143138195
            3804-3890           JAXUCZ010000003.1  143138764-143138850
            3891-5485           JAXUCZ010000003.1  143222448-143224042
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q36"
     gene            1..5485
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /note="phospholipase C beta 1"
                     /db_xref="GeneID:24654"
                     /db_xref="RGD:3344"
     exon            1..566
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     CDS             468..4118
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /EC_number="3.1.4.11"
                     /note="PLC-I; PLC-154; PLC-beta-1; phosphoinositide
                     phospholipase C; phospholipase C-I; phosphoinositide
                     phospholipase C-beta-1; Phospholipase C-beta1;
                     phospholipase C, beta 1 (phosphoinositide-specific)"
                     /codon_start=1
                     /product="1-phosphatidylinositol 4,5-bisphosphate
                     phosphodiesterase beta-1"
                     /protein_id="NP_001071109.1"
                     /db_xref="GeneID:24654"
                     /db_xref="RGD:3344"
                     /translation="MAGAQPGVHALQLKPVCVSDSLKKGTKFVKWDDDSTIVTPIILR
                     TDPQGFFFYWTDQNKETELLDLSLVKDARCGKHAKAPKDPKLRELLDVGNIGHLEQRM
                     ITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATNLLAQNMSRDAFLEKAYTKLK
                     LQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPDVYRVFLNN
                     LCPRPEIDNIFSEFGAKSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIE
                     KYEPNSSLAKKGQMSVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTY
                     LTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVI
                     EAIAECAFKTSPFPILLSFENHVDSPKQQAKMAEYCRLIFGDALLMEPLEKYPLESGV
                     PLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTYSDSSSVFEPSSPGAGEAD
                     TESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFETSKKRNKSFE
                     MSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVAL
                     NFQTVDLAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKI
                     ISGQFLSDKKVGTYVEVDMFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLP
                     SLACLRIAAYEEGGKFIGHRILPVQAIRPGYHYICLRNERNQPLMLPAVFVYIEVKDY
                     VPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLEDEEEVKKEADPGETSSEAPSETR
                     TTPAENGVNHTATLAPKPPSQAPHSQPAPGSVKAPAKTEDLIQSVLTEVEAQTIEELK
                     QQKSFVKLQKKHYKEMKDLVKRHHKKTTELIKEHTTKYNEIQNDYLRRRAALEKSAKK
                     DSKKKSEPSSPDHGSSAIEQDLAALDAEMTQKLIDLKDKQQQQLLNLRQEQYYSEKYQ
                     KREHIKLLIQKLTDVAEECQNNQLKKLKEICEKEKKELKKKMDKKRQEKITEAKSKDK
                     SQMEEEKTEMIRSYIQEVVQYIKRLEEAQSKRQEKLVEKHKEIRQQILDEKPKLQMEL
                     EQEYQDKFKRLPLEILEFVQEAMKGKVSEDSNHGSAPPSLASDPAKVNLKSPSSEEVQ
                     GENAGREFDTPL"
     exon            567..644
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            645..713
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            714..851
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            852..931
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            932..985
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1061
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            1062..1162
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            1163..1329
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            1330..1476
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            1477..1634
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            1635..1717
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            1718..1802
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            1803..1980
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            1981..2048
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            2049..2145
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            2146..2230
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            2231..2355
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            2356..2510
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            2511..2675
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            2676..2775
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            2776..2880
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            2881..2990
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            2991..3123
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            3124..3177
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            3178..3397
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            3398..3578
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            3579..3655
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            3656..3745
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            3746..3803
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            3804..3890
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
     exon            3891..5485
                     /gene="Plcb1"
                     /gene_synonym="Phosphb; PLC'1; PLCbeta1; RATPHOSPHB"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgcaaagttg ggcagggagc tgggggagga ggaccgcgag cgagggtggc ggagcagggg
       61 cgggaggagc ggagggagga ggggaccgga gcgtgtcact cgcgcgctcc ctctgtgcac
      121 agaggatgtg ctgaatggtg cgcttccagg cggcggccga gcaggatcag gcggcgggcg
      181 gctcgcactg ccgggctctg ctccttcttg tctcccgagg ctctacaatc accgcgggct
      241 ccagaccctg cgtcccgccc ggggcatggc aggctgctgt gccttcgcgt gtagtcccgc
      301 ttactaagcg gcgcgggcca gaggtgcgga ggccaagagg ccgggaggtc ggcgggcagc
      361 ggaggcaaga gcctgctgaa ccgagagcca agcccgcttg cgcccggagc tccgtgtccc
      421 gtctccactg cgctcgcccg ggccgcccgg agccccgatg agcccagatg gctggggctc
      481 agcccggagt gcacgccttg caactcaagc ccgtgtgcgt gtccgacagc ctcaagaagg
      541 gcaccaaatt cgtcaagtgg gatgatgact ccactatagt tactccaatt attttgagga
      601 ccgatcctca gggatttttc ttttactgga cagatcagaa taaggagacg gagctgttag
      661 atctcagcct cgtcaaggat gccaggtgtg ggaagcacgc caaagctccc aaggacccca
      721 agttacgtga acttctggat gttgggaaca tcggacactt ggaacagcgc atgataactg
      781 tggtgtatgg gccagacctg gtgaatatct cccacctgaa tcttgtggct tttcaagagg
      841 aagtggccaa ggaatggaca aatgaggttt tcagtttggc aacaaacctg ctggctcaga
      901 acatgtccag ggacgcattt ctggagaaag catatactaa gctcaagctt caggtgaccc
      961 cagaagggcg cattcctctt aaaaacatct atcgactgtt ttcggcagac cggaagcgag
     1021 tggaaactgc gctagaggcc tgtagtcttc catcgtcaag gaacgattcc atccctcaag
     1081 aggactttac tccagatgta tacagagttt tcctgaacaa tctttgtccc cgacctgaaa
     1141 ttgataacat cttctctgaa tttggtgcca aaagcaaacc gtaccttact gttgatcaga
     1201 tgatggattt tatcaacctt aagcagagag atccccggct gaatgaaata ctttacccac
     1261 ctctgaagca agagcaggtc caagtgttga ttgagaagta cgagcccaac agcagcctcg
     1321 ccaagaaagg gcagatgtca gtggatggat tcatgcgcta cctgagcgga gaagaaaatg
     1381 gagtcgtttc acctgagaaa ctggatttga acgaagacat gtctcagccc ctgtctcact
     1441 atttcatcaa ttcctcacac aacacctacc tcacagctgg ccagttggct gggaactcgt
     1501 ctgtagagat gtatcgccag gtgcttctgt ctggatgtcg ctgtgtggag ctggactgct
     1561 ggaagggcag gaccgctgag gaagagcctg tcatcaccca tggattcacc atgacaacag
     1621 aaatatcctt caaggaagtc atagaagcca tcgcagagtg tgcgttcaag acttctcctt
     1681 ttcccatcct cctttccttt gagaaccatg tggattcccc gaagcaacaa gccaagatgg
     1741 ccgagtattg ccgattaatc tttggtgatg ccctccttat ggagccactg gaaaaatacc
     1801 cactggaatc tggggtacct cttccaagcc ctatggattt aatgtataaa atcttggtga
     1861 aaaacaagaa gaagtcacac aagtcgtcag agggaagtgg taaaaagaag ctctctgagc
     1921 aagcttccaa cacgtacagc gactcttcca gcgtgttcga gccttcgtct ccgggagctg
     1981 gggaagcaga tacggagagt gatgacgatg acgacgatga tgactgtaaa aagtcttcca
     2041 tggatgaggg gactgctggc agcgaggcca tggccacaga agagatgtct aacctggtga
     2101 actatattca gcctgtcaag tttgagtcct ttgaaacttc aaaaaaaaga aataaaagct
     2161 ttgaaatgtc ttccttcgtg gaaaccaaag gactcgaaca actcacgaag tctccagttg
     2221 aatttgtcga atacaacaag atgcagctta gcaggatata tcccaaagga acacgcgtgg
     2281 actcatccaa ctacatgcct cagctcttct ggaatgctgg ctgtcagatg gtggcgctca
     2341 acttccagac agtggatcta gctatgcaga taaacatggg catgtacgaa tacaatggga
     2401 agagtggcta caggctgaag ccagagttca tgaggaggcc agacaagcat tttgatccat
     2461 ttactgaagg aatcgtagat gggatagtgg ccaacacttt atctgttaag attatttcag
     2521 gtcagtttct ctctgataag aaagttggga cttatgtgga agtggatatg tttggtttgc
     2581 ctgtggacac aagaaggaag gcatttaaaa ccaagacatc ccaaggaaat gctgtaaatc
     2641 ctgtctggga agaagagcca attgtattca aaaaggtagt tctgccttct ctggcctgtt
     2701 taaggatagc agcatatgag gaaggaggca aatttattgg ccaccggatc ttgcctgtgc
     2761 aagcaattcg gccaggctat cactacatct gcctgcggaa tgagaggaac cagcccctga
     2821 tgctgccagc tgtctttgtc tacatagaag tcaaagacta tgtcccagac acgtatgcag
     2881 atgtaattga agcattatca aacccaatcc gatatgtcaa tctgatggaa cagagagcta
     2941 agcagttggc tgcattgaca ctggaggatg aagaggaagt caagaaggag gctgaccccg
     3001 gggaaacgtc atccgaggct ccaagtgaaa ccaggacaac tccagcagag aatggggtga
     3061 atcacaccgc aacccttgca cccaagccac cttcccaggc tccacacagc cagcctgctc
     3121 cagggtctgt gaaggcaccc gccaaaacag aggatctgat tcagagcgtg ttaacagaag
     3181 tagaggcgca gaccattgaa gagctcaagc aacagaaatc gttcgtgaaa cttcaaaaga
     3241 agcactacaa agaaatgaaa gacctggtca agagacacca caagaaaacc accgagctca
     3301 tcaaggagca taccaccaag tacaatgaga ttcagaatga ctacttgaga aggagggcag
     3361 ccttggaaaa gtccgccaaa aaggatagca agaagaaatc tgaacccagc agcccagatc
     3421 atggctcatc cgccattgag caagacctcg cggccctgga tgcagaaatg actcagaagt
     3481 tgatagactt gaaagacaag caacaacagc agctgcttaa tcttcggcaa gagcagtatt
     3541 acagtgagaa gtaccagaag cgggagcaca ttaaattgct cattcagaag ttgacagatg
     3601 ttgctgaaga gtgtcagaac aatcagttga agaagctgaa ggaaatctgc gagaaagaga
     3661 agaaggaatt aaagaagaaa atggataaga agaggcagga gaagataaca gaagccaagt
     3721 ccaaagacaa aagccagatg gaagaggaga agacagagat gatccgatca tacatccagg
     3781 aggtggtgca gtacatcaag aggttagagg aagcacaaag taaaagacaa gaaaaacttg
     3841 tggaaaaaca caaggagatc cgccagcaga tcctggatga gaagcccaag ctgcagatgg
     3901 agctggagca agaataccaa gacaagttca aaagactgcc cctggagatt ctggagtttg
     3961 tacaggaagc catgaaaggg aaggttagtg aggacagcaa tcacggctct gcccctccct
     4021 cgctggcctc agaccctgct aaggtgaacc tcaagtctcc ctccagtgag gaggtacaag
     4081 gagagaacgc gggaagagag tttgatactc ctctgtgatg tccctgccgg gcctaccaga
     4141 catgcacggc tgcttgaact ccatcggact ctaaagacaa agatcactgc ccgggccatc
     4201 ttcctgagaa acatccctta gcctgaaatc cacaccaaag ggagagttcc agaaggatcc
     4261 gtgtgaaggt cccataccct ggtcccttgt gtcatgtgga aactattgcg gtcttagaga
     4321 gaagggtgca tgtatgcagg atttttcttt tcgttccaat agtaaattaa aagcaggcag
     4381 ctccaggctc catggactat gtaatgaagg acaatgtctt ctttgaagaa aactagagct
     4441 cgtgtcttcg tttgaagccc tggtgtacag tatttccaag taaaagagag agtttgagaa
     4501 gtgcgcggca ccatttaaca ctctggaaca ttccactctg agcattgttt ccctgactgc
     4561 cccgcaaacc catgttttca agttaacgtg tatagtgcat tgtttcaccc tttgctttgc
     4621 aggcactggt ggcttgccat ttgctaattt atttatccca gaggcatctg tatttgctat
     4681 tgacatggct ttattagata ccatagtgac tcatataagc tggtttcttt tataaaaaaa
     4741 aatcacatga ctgtatcatt tcccagtgaa gccattttac gattagcaat atgggttgta
     4801 tatttggctg cacgtctttg gatatctatc ggttgttgac ctgaggctat caaacagctg
     4861 caatgggttt gttctgttga gtcagggttt ccgccagact gaacaggatc cagtgggcta
     4921 ttttattgtc agatattttt tcgtggttca tttattttta ctgtagaaag gaagatagaa
     4981 tatttatcta aatgcacatg tataaatgat atacattatc tccatgtata tagtacatgt
     5041 atgcacccag ccatataagt gcacacacat acatacgcat gagtgtacat gtgtgtttat
     5101 taattggcag tgacccaaat ctcttccata agatttaaaa gcaagttcag ggatacatgg
     5161 acagagaaga aatgggtcga atatatgtta tgtggataat taaattatac ggaaatgcta
     5221 agaatcagtt tatggagtca atgatgcaac ttgaggtaat cttagaattt agaaaatgag
     5281 tttactaaaa aaattggagg atcaaaaatt ggattttaag tatcagattt tgagcttgtt
     5341 ttaatggtca gtgtatgcaa aaatgcatag gaaatagagt gcaaatgtat tactactatc
     5401 ttcaataaat tttaactgaa taatttcaat tttatactga tagaaataaa gctctggttg
     5461 tattttagtt tgtatattta cttca
//
